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    METHODS AND COMPOSITIONS FOR GENERATING EPICARDIUM CELLS
    51.
    发明专利
    METHODS AND COMPOSITIONS FOR GENERATING EPICARDIUM CELLS 未知

    公开(公告)号：SG11201601922PA

    公开(公告)日：2016-04-28

    申请号：SG11201601922P

    申请日：2014-09-12

    Applicant: UNIV HEALTH NETWORK

    Inventor： KELLER GORDON ,  WITTY ALEC DRAKE ,  KATTMAN STEVEN JAMES

    IPC: C12N5/077 ,  C12Q1/68 ,  G01N33/50

    Abstract: Methods and products for obtaining cardiovascular lineage cells from hPSCs. The method comprises one or more of the following steps: (a) contacting BMP component primed hPSCs with a cardiovascular mesoderm programming cocktail and culturing the contacted hPSCs for a period of time to generate a KDR+ and PDGFRalpha+ cardiovascular mesoderm cell population; (b) contacting the cardiovascular mesoderm cell population with a cardiovascular progenitor specification cocktail and culturing the contacted cardiovascular mesoderm cell population for a period of time to generate a NKX2-5+ or WT1+ cardiovascular progenitor cell population; and (c) contacting the cardiovascular progenitor cell population with a maturation cocktail and culturing the contacted cardiovascular progenitor population for a period of time to produce a cardiovascular population optionally cardiomyocyte lineage cells expressing cardiac troponin T (cTnT) and/or SIRPA and/or epicardial lineage cells expressing WT1.

    METHODS AND COMPOSITIONS FOR GENERATING EPICARDIUM CELLS
    53.
    发明专利
    METHODS AND COMPOSITIONS FOR GENERATING EPICARDIUM CELLS 未知

    公开(公告)号：CA2921081A1

    公开(公告)日：2015-03-19

    申请号：CA2921081

    申请日：2014-09-12

    Applicant: UNIV HEALTH NETWORK

    Inventor： KATTMAN STEVEN JAMES ,  WITTY ALEC DRAKE ,  KELLER GORDON

    IPC: C12N5/077 ,  C12Q1/68 ,  G01N33/50

    Abstract: Provided are methods and products for obtaining cardiovascular lineage cells from hPSCs. The method for obtaining a cardiomyocyte lineage or an epicardial lineage cell population from human pluripotent stem cells (hPSCs) comprises one or more of the following steps: (a) contacting BMP component primed hPSCs with a cardiovascular mesoderm programming cocktail suitable for inducing the hPSCs to differentiate to a cardiovascular mesoderm cell population under conditions suitable for the programming cocktail to penetrate the hPSCs and culturing the contacted hPSCs for a period of time to generate a KDR+ and PDGFRalpha+ cardiovascular mesoderm cell population; (b) contacting the cardiovascular mesoderm cell population with a cardiovascular progenitor specification cocktail suitable to specify a NKX2-5+ or WT1+ cardiovascular progenitor cell population under conditions suitable for the specification cocktail to penetrate the cardiovascular mesoderm cell population and culturing the contacted cardiovascular mesoderm cell population for a period of time to generate a NKX2-5+ or WT1+ cardiovascular progenitor cell population; and (d) contacting the cardiovascular progenitor cell population with a maturation cocktail under conditions suitable for the maturation cocktail to penetrate the cardiovascular progenitor cell population and culturing the contacted cardiovascular progenitor population for a period of time to produce a cardiovascular population optionally cardiomyocyte lineage cells expressing cardiac troponin T (cTnT) and/or SIRPA and/or epicardial lineage cells expressing WT1.

    Methods and compositions for generating chondrocyte lineage cells and/or cartilage like tissue
    56.
    发明专利
    Methods and compositions for generating chondrocyte lineage cells and/or cartilage like tissue 未知

    公开(公告)号：NZ713111A

    公开(公告)日：2021-10-29

    申请号：NZ71311114

    申请日：2014-04-02

    Applicant: UNIV HEALTH NETWORK

    Inventor： KELLER GORDON ,  CRAFT APRIL M

    IPC: C12N5/077 ,  A61K35/32 ,  A61P19/00 ,  C12N5/073 ,  C12Q1/02 ,  G01N33/48 ,  G01N33/50

    Abstract: A method for generating chondrocytes and/or cartilage, optionally articular like non-hypertrophic chondrocyte cells and/or cartilage like tissue and/or hypertrophic chondrocyte like cells and/or cartilage like tissue, the method comprising: a. culturing a primitive streak-like mesoderm population, optionally a CD56+, PDGFRalpha+ KDR- primitive streak-like mesoderm population, with a paraxial mesoderm specifying cocktail comprising: i. a FGF agonist; ii. a BMP inhibitor; optionally Noggin, LDN-193189, Dorsomorphin; and iii. optionally one or more of a TGFbeta inhibitor, optionally SB431524; and a Wnt inhibitor, optionally DKK1, IWP2, or XAV939; to specify a paraxial mesoderm population expressing cell surface CD73, CD105 and/or PDGFR-beta; b. generating a chondrocyte precursor population comprising: i. culturing the paraxial mesoderm population expressing CD73, CD105 and/or PDGFR-beta at a high cell density optionally in serum free or serum containing media; ii. culturing the high cell density CD73+, CD105+ and/or PDGFRbeta+ paraxial mesoderm population with a TGFbeta3 agonist in serum free media to produce a high cell density Sox9+, collagen 2+ chondrocyte precursor population; and c. either i. culturing the high cell density Sox9+, collagen 2+ chondrocyte precursor population with the TGFbeta3 agonist for an extended period of time to produce an articular like non-hypertrophic chondrocyte cells and/or cartilage like tissue; or ii. culturing the high cell density Sox9+ collagen2+ chondrocyte precursor population with a BMP4 agonist for an extended period of time to produce a hypertrophic chondrocyte like cells and/or cartilage like tissue.

    GENERATING ATRIAL AND VENTRICULAR CARDIOMYOCYTE LINEAGES FROM HUMAN PLURIPOTENT STEM CELLS
    58.
    发明专利
    GENERATING ATRIAL AND VENTRICULAR CARDIOMYOCYTE LINEAGES FROM HUMAN PLURIPOTENT STEM CELLS 未知

    公开(公告)号：SG10202105977WA

    公开(公告)日：2021-07-29

    申请号：SG10202105977W

    申请日：2017-12-04

    Applicant: UNIV HEALTH NETWORK

    Inventor： KELLER GORDON ,  PROTZE STEPHANIE ,  LEE JEE HOON

    Abstract: Methods are disclosed for producing populations of cardiomyocytes from pluripotent stem cells. Populations may be enriched for either atrial or ventricular cardiomyocytes and the resulting ventricular population may be essentially free of pacemaker cells. The method includes incubating pluripotent stem cells in a suitable medium with a BMP component, and an activin component, the amounts of activin may be varied to enrich for either atrial or ventricular cardiomyocytes. The enriched populations, as well as methods of using the same to treat patients in need of cardiac repair are disclosed.

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