公开(公告)号：WO1997035203A1

公开(公告)日：1997-09-25

申请号：PCT/US1997004378

申请日：1997-03-19

Applicant: UNIVERSITY OF UTAH RESEARCH FOUNDATION ,  HERRON, James, N. ,  CHRISTENSEN, Douglas, A. ,  MILES, Scott, D.

Inventor： UNIVERSITY OF UTAH RESEARCH FOUNDATION

IPC: G01N33/552

CPC classification number: G01N33/54386 ,  G01N33/54373 ,  Y10S435/808 ,  Y10S436/807

Abstract: An apparatus and method for rapidly analyzing samples for analytes of interest by an homogeneous immunofluorescence assay. The apparatus includes a sample test cartridge having a high control sample section, a low control sample section, and at least one test sample section. Each of these sections contain at least one pre-loaded reagent housed in a well within the the cartridge wherein the low control sample section contains a known low amount of an analyte of interest and the high control sample section contains a known high amount of an analyte of interest. The cartridge includes a biosensor comprising a planar waveguide having first and second parallel plane surfaces and an edge extending between them, the edge having a receiving region for receiving a light beam. Each of the high control sample section, the low control sample section, and the test sample control sections have a well which includes a waveguide surface, wherein the contents of each section contacts capture molecules immobilized on the waveguide surface. The capture molecules are configured to specifically bind a chosen analyte and fluoresce when interacting with light passing through the waveguide surface. The concentration of said analyte of interest in said sample fluid is determined by a comparison of intensities of fluorescence of between said capture molecule areas of said sample capture molecule well, said low control capture molecule well, and said high control capture molecule well.

Abstract translation: 用于通过均匀免疫荧光测定快速分析感兴趣的分析物的样品的装置和方法。 该装置包括具有高控制样品部分，低对照样品部分和至少一个测试样品部分的样品测试盒。 这些部分中的每一个包含至少一个预先装载的试剂，其容纳在筒内的孔中，其中低对照样品部分含有已知的低量感兴趣的分析物，并且高对照样品部分含有已知的大量分析物 出于兴趣。 所述盒包括生物传感器，所述生物传感器包括具有第一和第二平行平面的平面波导和在它们之间延伸的边缘，所述边缘具有用于接收光束的接收区域。 高控制采样部分，低控制样品部分和测试样品控制部分中的每一个具有包括波导表面的阱，其中每个部分的内容物接触固定在波导表面上的捕获分子。 捕获分子被配置为特异性地结合所选择的分析物并且当与通过波导表面的光相互作用时发出荧光。 通过比较所述样品捕获分子阱的所述捕获分子区域，所述低对照捕获分子阱和所述高对照捕获分子阱之间的荧光强度来确定所述样品流体中所述目标分析物的浓度。

公开(公告)号：WO1997035181A1

公开(公告)日：1997-09-25

申请号：PCT/US1997004377

申请日：1997-03-19

Applicant: UNIVERSITY OF UTAH RESEARCH FOUNDATION ,  HERRON, James, N. ,  CHRISTENSEN, Douglas, A.

Inventor： UNIVERSITY OF UTAH RESEARCH FOUNDATION

IPC: G01N21/64

CPC classification number: G01N21/648 ,  G01N21/6452 ,  G01N33/54373

Abstract: The present invention relates to a system (80) for determining analyte concentration. The system (80) includes an optical detection system (92) that detects fluorescence from fluorescent binding assays in a biosensor (88). A processing system (96) may be used to determine analyte concentration from the fluorescence detected by the optical detection system (92). The optical detection system (92) may include photodetectors with or without in series lenses. Alternatively, a CCD camera (146) may be used.

Abstract translation: 本发明涉及一种用于确定分析物浓度的系统（80）。 系统（80）包括在生物传感器（88）中检测来自荧光结合测定的荧光的光学检测系统（92）。 处理系统（96）可用于根据由光学检测系统（92）检测的荧光来确定分析物浓度。 光学检测系统（92）可以包括具有或不具有串联透镜的光电探测器。 或者，可以使用CCD照相机（146）。

公开(公告)号：WO1997023632A1

公开(公告)日：1997-07-03

申请号：PCT/US1996019917

申请日：1996-12-20

Applicant: UNIVERSITY OF UTAH RESEARCH FOUNDATION ,  GENZYME GENETICS

Inventor： UNIVERSITY OF UTAH RESEARCH FOUNDATION ,  GENZYME GENETICS ,  KEATING, Mark, F. ,  CURRAN, Mark, E. ,  LANDES, Gregory, M. ,  CONNORS, Timothy, D.

IPC: C12N15/63

CPC classification number: C07K14/47 ,  A01K2217/05 ,  A61K48/00 ,  C07K14/705

Abstract: One aspect of the invention relates to the identification of the molecular basis of long QT syndrome. More specifically, the invention has identified that mutated KVLQT1 causes long QT syndrome. The analysis of this gene will provide an early diagnosis of subjects with long QT syndrome. The diagnostic methods comprise analyzing the nucleic acid sequence of the KVLQT1 gene of an individual to be tested and comparing them with the nucleic acid sequence of the native, non-variant gene. Alternatively, the amino acid sequence of KVLQT1 may be analyzed for mutations which cause long QT syndrome. Presymptomatic diagnosis of long QT syndrome will enable practitioners to treat this disorder using existing medical therapy. A second aspect of the invention relates to the realization that KVLQT1 coassembles with minK to form a cardiac potassium channel. This allows one to assay for drugs which interact with this channel to identify new drugs which are useful for treating or preventing long QT.

Abstract translation: 本发明的一个方面涉及长QT综合征的分子基础的鉴定。 更具体地，本发明已经鉴定出突变的KVLQT1引起长QT综合征。 该基因的分析将为长期QT综合征患者提供早期诊断。 诊断方法包括分析待测个体的KVLQT1基因的核酸序列，并将其与天然非变异基因的核酸序列进行比较。 或者，可以分析KVLQT1的氨基酸序列引起长QT综合征的突变。 长期QT综合征的症状诊断将使实践者能够使用现有的药物治疗来治疗这种疾病。 本发明的第二方面涉及KVLQT1与minK组装以形成心脏钾通道的实现。 这可以测定与该通道相互作用的药物，以鉴定可用于治疗或预防长QT的新药物。

公开(公告)号：WO1997020947A1

公开(公告)日：1997-06-12

申请号：PCT/US1996019472

申请日：1996-12-05

Applicant: UNIVERSITY OF UTAH RESEARCH FOUNDATION ,  ANDRADE, Joseph, D. ,  WANG, Chung-Yih ,  HLADY, Vladimir ,  TRIOLO, Philip, M. ,  SCHEER, Robert, J.

Inventor： UNIVERSITY OF UTAH RESEARCH FOUNDATION

IPC: C12Q01/66

CPC classification number: G01N21/763 ,  G01N21/6456 ,  G01N21/76

Abstract: A method and associated apparatus for measuring chemical concentration in a liquid sample based on spatial separation and resolution of light is disclosed. The method is preferably applied to sensitive, quantitative, luminescence-based biosensors which reads the analyte concentration via spatial distribution of the emitted light. The detection of light is used to assess the spatial position, rather than the intensity or wavelength, of emitted light. A bioluminescent or chemiluminescent reaction requiring, for example, ATP, NADPH or NADH as a specific, and sensitive co-factor is used. ATP or NADH concentration is modulated, "tuned" and/or regulated via, for example, an enzyme which consumes (consumase) ATP, NADPH, or NADH, thereby producing a spatial distribution of ATP or NADH and a spatial distribution in the emitted light. By appropriate control of the consumase or "synthase" activity and kinetics, a sensitive, specific, and easy readable luminescent pattern is produced, permitting detection. The method is applicable to a wide range of analytes, biochemicals and substrates by use of additional substrate-specific enzymes dependent on ATP concentration for their activity. The figure graphically depicts a single channel ATP sensor, where a specific ATP sample (22) is contacted with the ATP consumase immobilized in or on a hydrophilic gel matrix (24) causing a lower concentration of ATP to enter the transduction region (26) and react with, for example luciferase and luciferin, photons emitted and detected by operator (28).

Abstract translation: 公开了一种基于空间分离和光分辨率来测量液体样品中化学浓度的方法和相关装置。 该方法优选地应用于通过发射光的空间分布读取分析物浓度的灵敏的，定量的，基于发光的生物传感器。 光的检测用于评估发射光的空间位置，而不是强度或波长。 使用需要例如ATP，NADPH或NADH作为特异性和敏感辅因子的生物发光或化学发光反应。 ATP或NADH浓度被调节，通过例如消耗（消耗）ATP，NADPH或NADH的酶调节和/或调节，从而产生ATP或NADH的空间分布和发射光中的空间分布 。 通过适当控制消耗或“合成酶”活性和动力学，产生敏感，特异和容易读取的发光图案，允许检测。 该方法适用于广泛的分析物，生物化学物质和底物，通过使用依赖于其ATP浓度的其他活性的底物特异性酶。 该图图形地描绘了单通道ATP传感器，其中特定ATP样品（22）与固定在亲水凝胶基质（24）中或上的ATP消耗物接触，导致较低浓度的ATP进入转导区（26）和 与例如荧光素酶和荧光素，由操作者发射和检测的光子（28）反应。

公开(公告)号：WO1997014421A1

公开(公告)日：1997-04-24

申请号：PCT/US1996016689

申请日：1996-10-18

Applicant: UNIVERSITY OF UTAH RESEARCH FOUNDATION ,  WYETH-AYERST RESEARCH ,  IRELAND, Chris, M. ,  MAIESE, William, M.

Inventor： UNIVERSITY OF UTAH RESEARCH FOUNDATION ,  WYETH-AYERST RESEARCH

IPC: A61K31/70

CPC classification number: A61K31/704 ,  C07H15/203 ,  C07H21/00 ,  C12P19/44

Abstract: An antitumor antibiotic, namenamicin, purified from the marine ascidian Polysyncraton lithostrotum is disclosed. An antitumor composition for treating cancerous tumors comprises namenamicin and an inert carrier. A method of treating an individual for cancer comprises administering the composition comprising namenamicin and an inert carrier. A method of purifying namenamicin, a method of inhibiting the growth of a microorganism, and a method of cleaving DNA at a sequence-specific site are also described.

Abstract translation: 公开了一种从海洋中提取的抗肿瘤抗生素，纳马霉素。 用于治疗癌性肿瘤的抗肿瘤组合物包括纳马霉素和惰性载体。 治疗个体用于癌症的方法包括施用包含纳马霉素和惰性载体的组合物。 还描述了纯化纳摩霉素的方法，抑制微生物生长的方法以及在序列特异性位点切割DNA的方法。

公开(公告)号：WO1997007474A1

公开(公告)日：1997-02-27

申请号：PCT/US1996013486

申请日：1996-08-21

Applicant: UNIVERSITY OF UTAH RESEARCH FOUNDATION

Inventor： UNIVERSITY OF UTAH RESEARCH FOUNDATION ,  THOMAS, Charles, L. ,  WANG, Zetian

IPC: G06F19/00

CPC classification number: B29C64/141 ,  B29C33/12 ,  B33Y10/00 ,  B33Y50/02

Abstract: A rapid prototype modeling system operates to first electronically decompose a discrete part represented by a stereolithography file into thick layers (36), which are then electronically sliced into cross-sectional slices (39) the thickness of a sheet (38) of construction material. The slices (39) are cut from sheets (38) of the construction material in a pattern which permits construction of the layers (36) by stacking the sheets (38). The layers (36) are then stacked appropriately to create a physical model of the discrete part.

Abstract translation: 快速原型建模系统操作以首先将由立体光刻文件表示的分立部分电子分解成厚层（36），然后将其电切割成横截面（39）构造材料片材（38）的厚度。 切片（39）以允许通过堆叠片材（38）构造层（36）的图案从建筑材料的片材（38）切割。 然后将层（36）适当堆叠以产生离散部分的物理模型。

公开(公告)号：WO1996028537A1

公开(公告)日：1996-09-19

申请号：PCT/US1996003186

申请日：1996-03-08

Applicant: UNIVERSITY OF UTAH RESEARCH FOUNDATION

Inventor： UNIVERSITY OF UTAH RESEARCH FOUNDATION ,  KEATING, Mark, T. ,  SANGUINETTI, Michael, C. ,  CURRAN, Mark, E. ,  WANG, Quing

IPC: C12C03/00

CPC classification number: C12Q1/6883 ,  A61K38/00 ,  C07K14/705 ,  C12Q2600/156 ,  C12Q2600/172

Abstract: The invention relates to the identification of the molecular basis of acquired long QT syndrome. More specifically, the invention has identified that HERG is associated with acquired long QT syndrome. HERG has been identified to encode the major subunit for the IKr channel. It has been found that increasing the extracellular levels of K paradoxically increases the outward current of this channel. Increasing extracellular K levels in patients, especially those being treated with antiarrhythmic medication or other medication which can cause cardiac arrhythmia or those with the hereditary form of long QT syndrome, decreases the likelihood of developing LQT and torsade de pointes.

Abstract translation: 本发明涉及获得性长QT综合征的分子基础的鉴定。 更具体地，本发明已经确定HERG与所获得的长QT综合征相关。 已确定HERG编码IKr通道的主要亚单位。 已经发现，增加K +的细胞外水平矛盾地增加了该通道的向外电流。 增加患者的细胞外K +水平，特别是那些用抗心律失常药物治疗或可引起心律失常的其他药物或具有遗传性长QT综合征的患者，可降低LQT和尖端扭转型室性心动过速的可能性。

公开(公告)号：WO1996005307A2

公开(公告)日：1996-02-22

申请号：PCT/US1995010203

申请日：1995-08-11

Applicant: MYRIAD GENETICS, INC. ,  UNIVERSITY OF UTAH RESEARCH FOUNDATION ,  UNITED STATES OF AMERICA, represented by THE SECRETARY OF THE DEPARTMENT OF HEALTH AND HUMAN SERVICES

Inventor： MYRIAD GENETICS, INC. ,  UNIVERSITY OF UTAH RESEARCH FOUNDATION ,  UNITED STATES OF AMERICA, represented by THE SECRETARY OF THE DEPARTMENT OF HEALTH AND HUMAN SERVICES ,  SKOLNICK, Mark, H. ,  GOLDGAR, David, E. ,  MIKI, Yoshio ,  SWENSON, Jeff ,  KAMB, Alexander ,  HARSHMAN, Keith, D. ,  SHATTUCK-EIDENS, Donna, M. ,  TAVTIGIAN, Sean, V. ,  WISEMAN, Roger, W. ,  FUTREAL, Andrew, P.

IPC: C12N15/12

CPC classification number: C07K14/4703 ,  A01K2217/05 ,  A61K38/00 ,  A61K48/00 ,  C07K14/82 ,  C12Q1/6886 ,  C12Q2600/136 ,  C12Q2600/172

Abstract: The present invention relates generally to the field of human genetics. Specifically, the present invention relates to methods and materials used to isolate and detect a human breast and ovarian cancer predisposing gene (BRCA1), some mutant alleles of which cause susceptibility to cancer, in particular breast and ovarian cancer. More specifically, the invention relates to germline mutataions in the BRCA1 gene and their use in the diagnosis of predisposition to breast and ovarian cancer. The present invention further relates to somatic mutations in the BRCA1 gene in human breast and ovarian cancer and their use in the diagnosis and prognosis of human breast and ovarian cancer. Additionally, the invention relates to somatic mutations in the BRCA1 gene in other human cancers and their use in the diagnosis and prognosis of human cancers. The invention also relates to the therapy of human cancers which have a mutation in the BRCA1 gene, including gene therapy, protein replacement therapy and protein mimetics. The invention further relates to the screening of drugs for cancer therapy. Finally, the invention relates to the screening of the BRCA1 gene for mutations, which are useful for diagnosing the predisposition to breast and ovarian cancer.

Abstract translation: 本发明一般涉及人类遗传学领域。 具体地，本发明涉及用于分离和检测人乳腺癌和卵巢癌易感基因（BRCA1）的一些突变等位基因，特别是乳腺癌和卵巢癌的易感性的突变等位基因的方法和材料。 更具体地，本发明涉及BRCA1基因中的种系变异及其在诊断乳腺和卵巢癌易感性中的用途。 本发明还涉及人乳腺癌和卵巢癌中BRCA1基因的体细胞突变及其在人乳腺癌和卵巢癌诊断和预后中的应用。 另外本发明涉及其他人类癌症中BRCA1基因的体细胞突变及其在人类癌症诊断和预后中的应用。 本发明还涉及在BRCA1基因中具有突变的人类癌症的治疗，包括基因治疗，蛋白质替代疗法和蛋白质模拟物。 本发明还涉及用于癌症治疗的药物的筛选。 最后，本发明涉及用于突变的BRCA1基因的筛选，其可用于诊断乳腺和卵巢癌的易感性。

公开(公告)号：WO1996002192A1

公开(公告)日：1996-02-01

申请号：PCT/US1995008855

申请日：1995-07-19

Applicant: UNIVERSITY OF UTAH RESEARCH FOUNDATION

Inventor： UNIVERSITY OF UTAH RESEARCH FOUNDATION ,  CHASAN, Paul, E.

IPC: A61B17/00

CPC classification number: A61B90/39 ,  A61B2090/395

Abstract: An apparatus and method are disclosed for marking the proper location of incisions to be made during a surgical procedure and for coloring the skin. The apparatus includes a pinwheel (14) with a plurality of marking points (18) extending therefrom for penetrating the outermost layer of the epidermis. A reservoir supplies a marking agent so that as the penetrating member penetrates the epidermis, marking agent is left within the epidermis, thereby leaving a dotted line which may be followed when making incisions during surgery. The method includes rolling the pinwheel (14) across the epidermis, penetrating the outermost layer of the epidermis with points on the pinwheel and leaving marking agent carried by the point between the outermost layer of the epidermis and an innermost layer of the epidermis.

Abstract translation: 公开了一种设备和方法，用于标记在手术过程中要进行的切口的适当定位并使皮肤着色。 该装置包括具有从其延伸的多个标记点（18）的风车（14），用于穿透表皮的最外层。 储存器供应标记剂，使得当穿透构件穿透表皮时，标记剂留在表皮内，从而留下在手术期间进行切口时可以遵循的虚线。 该方法包括滚动风轮（14）穿过表皮，穿过针轮上的点穿过表皮的最外层，并留下由表皮的最外层与表皮的最内层之间的点携带的标记剂。

公开(公告)号：WO1995003429A1

公开(公告)日：1995-02-02

申请号：PCT/US1994008279

申请日：1994-07-22

Applicant: UNIVERSITY OF UTAH RESEARCH FOUNDATION

Inventor： UNIVERSITY OF UTAH RESEARCH FOUNDATION ,  WEI, Ai-Ping ,  HERRON, James, N.

IPC: C12Q01/68

CPC classification number: G01N33/582 ,  C12Q1/6818 ,  Y10S436/80 ,  Y10S436/805

Abstract: Fluorescent energy transfer dyes capable of moving between a more stacked configuration to exhibit fluorescent quenching and a more spaced configuration to exhibit fluorescence can be conjugated to a peptide epitope or nucleic acid for use in the detection of an unknown antibody in bulk solution. The resulting labelled peptide reagent can be used in an immunoassay procedure by placing it in bulk solution along with the unknown antibody to be detected. When the antibody binds to the peptide epitope, the pair of dyes carried by the peptide epitope will have their configuration altered from a stacked to an unstacked configuration and will exhibit a fluorescent increase in response thereto.

Abstract translation: 能够在更叠层的配置之间移动以展现荧光猝灭和更间隔的配置以显示荧光的荧光能量转移染料可以缀合到肽表位或核酸上，以用于检测本体溶液中的未知抗体。 所得标记的肽试剂可以在免疫测定程序中使用，将其与待检测的未知抗体一起放入本体溶液中。 当抗体与肽表位结合时，由肽表位携带的一对染料将具有从堆叠到未堆叠构型的改变，并且对其反应将呈现荧光增加。