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    RECYCLING OF ENZYMES FROM BIOREACTORS
    65.
    发明公开
    RECYCLING OF ENZYMES FROM BIOREACTORS 审中-公开
    回收酶从生物反应器

    公开(公告)号：EP2585605A1

    公开(公告)日：2013-05-01

    申请号：EP11728258.2

    申请日：2011-06-24

    Applicant: UPFRONT CHROMATOGRAPHY A/S

    Inventor： LIHME, Allan Otto Fog ,  STANLEY, Christopher John

    IPC: C12P7/02 ,  C12P7/06 ,  C12P7/08 ,  C12P7/10 ,  C12P7/16 ,  C12P7/62 ,  C12P7/64

    CPC classification number: C12P7/10 ,  B01D15/168 ,  B01D15/1892 ,  B01D15/3809 ,  C07K1/22 ,  C12P7/04 ,  C12P7/16 ,  C12P7/649 ,  C12P19/02 ,  C12P19/14 ,  Y02E50/16 ,  Y02E50/17

    Abstract: A method of conducting an enzymatic process in which the enzymes are recovered and reused in at least a second iteration of the process, wherein each iteration of the process comprises the steps of: (a) providing a heterogeneous substrate solution, in which the substrate is fully soluble, partially soluble or insoluble; (b) adding an enzyme or mixture of enzymes to the heterogeneous substrate solution; and (c) allowing an enzymatic reaction of the substrate to proceed; wherein, after completion of step (c) for each iteration, the enzyme is recovered from the mixture resulting from step (c) according to the following steps: (d) conducting a non-packed-bed adsorption process comprising contacting the reaction mixture with an adsorbent that adsorbs the enzyme in order to separate the enzyme from the reaction mixture resulting from step (c); (e) optionally washing unbound material from the adsorbent; and (f) desorbing the enzyme from the adsorbent; and further wherein the desorbed enzyme obtained in step (f) is used in step (b) of at least one subsequent iteration of the process.

    Isolation of immunoglobulins
    68.
    发明公开
    Isolation of immunoglobulins 失效
    Isolierung von Immunglobulinen

    公开(公告)号：EP2095873A1

    公开(公告)日：2009-09-02

    申请号：EP08169158.6

    申请日：1997-09-01

    Applicant: UPFRONT CHROMATOGRAPHY A/S

    Inventor： Hansen, Marie Bendix ,  Lihme, Allan Otto Fog

    IPC: B01J20/32 ,  B01D15/00 ,  B01J20/22

    CPC classification number: C07K16/00 ,  B01D15/1807 ,  B01D15/3804 ,  B01J20/3219 ,  B01J20/3242 ,  B01J20/3253 ,  B01J20/3255 ,  C07K1/22

    Abstract: The present invention relates to a method for the isolation of immunoglobulins from a solution containing one or more immunoglobulins, comprising the following operations: a) contacting the solution containing one or more immunoglobulins having a pH in the range of 2.0 to 10.0 and a total salt content corresponding to an ionic strength of at the most 2.0 with a solid phase matrix comprising a functionalised matrix backbone carrying a plurality of functional groups of the following formula: M-SP1-L; wherein M designates the matrix backbone; SP1 designates a spacer; L designates a ligand comprising a mono- or bicyclic optionally substituted aromatic or heteroaromatic moiety; with the proviso that the molecular weight of the ligand -L is at the most 500 Dalton,
    whereby at least a part of the immunoglobulins becomes bound to the solid phase matrix; and
    b) separating the solid phase matrix having immunoglobulins bound thereto from the solution; and
    c) optionally washing the solid phase matrix; and
    d) contacting the solid phase matrix with an eluent in order to liberate one or more of the immunoglobulins from the solid phase matrix."

    Abstract translation: 本发明涉及从含有一种或多种免疫球蛋白的溶液中分离免疫球蛋白的方法，其包括以下操作：a）使含有一种或多种pH在2.0至10.0范围内的一种或多种免疫球蛋白的溶液和总盐 相对于离子强度最高为2.0的含量为固相基质，所述固相基质包含具有多个下式的官能团的骨架主链：M-SP1-L; 其中M表示基质骨架; SP1表示间隔物; L表示包含单环或双环任选取代的芳族或杂芳族部分的配体; 条件是配体-L的分子量为至多500道尔顿，其中至少一部分免疫球蛋白结合到固相基质上; 和b）从所述溶液中分离具有与其结合的免疫球蛋白的固相基质; 和c）任选地洗涤固相基质; 和d）使固相基质与洗脱剂接触，以便从固相基质释放一种或多种免疫球蛋白。

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