Abstract:
A transformed cell line is provided to identify a chemical material controlling expression of an IDH3A gene at a transcription step and also find out an anti-obese material such as genistein. An expression vector pIDH3A comprises: a kanamycin resistant gene(Kana+) and a neomycin resistant gene(Neo+); a luciferase gene(LUC+); and an IDH3A promoter of SEQ ID : NO. 1 and has a structure depicted in Fig. 2. A cell line PRDC IDH3A transformed by the expression vector is deposited as a deposition no. KCTC 10981BP. A method for searching an obesity controlling material comprises the steps of: (a) treating the cell line with an obesity controlling candidate material; and (b) detecting the fluorescence emitting reaction, wherein the obesity controlling candidate material is treated with at least two concentrations.
Abstract:
A cosmetic composition for suppressing sebum comprising catechins and kaempferol is provided to reduce activity of PPAR-gamma(peroxisome proliferator-activated receptor gamma) and suppress production of sebum, so that acnes symptom is alleviated. The cosmetic composition for suppressing sebum comprises catechins and kaempferol derived from green tea leaf, wherein the catechin is selected from (-)EGCG(epigallocatechin gallate), (-)GCG(gallocatechin gallate), (-)ECG(epicatechin gallate), (-)CG(catechin gallate), (-)EGC(epigallocatechin), (-)GC(gallocatechin), (-)EC(epicatechin), (+)EC(epicatechin), (-)CA(catechin) and (+)CA(catechin). The mixing ratio of catechins and kaempferol is 1:0.1-10, and the amounts of catechins and kaempferol are 0.001-30 wt.% respectively based on the total weight of the composition.