Abstract:
PURPOSE: A method for producing high purity silk peptides using gel filtration is provided. The silk peptide solution or powder is applied to cosmetics, and food additives and hydrolyzed using protease to prepare silk peptide as pure as oligopeptide of hundreds-thousands units. CONSTITUTION: The method for producing high purity silk peptide comprises the steps of: obtaining silk fibroin from silk proteins under high temperature and pressure to remove sericin from silk proteins; dissolving silk fibroin in 50% CaCl2·H2O·ethanol solution at 95 deg. C for 5 hours; subjecting the silk fibroin solution to gel filtration chromatography packed with 1 to 150 micrometer, preferably 20 to 80 micrometer bead sized media, sephadex G-10 or G-25; and freeze-drying or spray drying the purified silk peptide solution by using a freezing dryer or spray dryer.
Abstract translation:目的:提供使用凝胶过滤生产高纯度丝肽的方法。 将丝肽溶液或粉末用于化妆品和食品添加剂,并使用蛋白酶水解制备丝素肽,纯度为数十万单位的寡肽。 构成:生产高纯度丝肽的方法包括以下步骤:在高温高压下从丝蛋白中获得丝心蛋白,从丝蛋白中去除丝胶蛋白; 将丝素蛋白溶解在95%的50%CaCl2·H2O·乙醇溶液中。 C 5小时; 将丝素蛋白溶液经1〜150微米,优选20〜80微米珠粒度培养基,sephadex G-10或G-25填充凝胶过滤色谱; 并使用冷冻干燥器或喷雾干燥器冷冻干燥或喷雾干燥纯化的丝肽溶液。
Abstract:
PURPOSE: A method for manufacturing soy sauce by using silk amino acid solution is provided to increase sugar content of soy sauce by including high concentration silk amino acid and free amino acid of 97.6% in the soy sauce. CONSTITUTION: A variety of soy sauce by using silk amino acid solution comprises the steps of: (1) gaining silk fibroin protein by removing sericin; (2) hydrolyzing the silk fibroin protein to be 50% of the concentration by adding 25% of hydrochloric acid to the silk fibroin protein at 98deg.C for 48hours, and gaining silk amino acid solution of pH 5.0-5.5 by adding sodium hydroxide of 9M; (3) decoloring and deodorizing the silk amino acid solution; and (4) passing the silk amino acid solution through an electric dialyzer or gel filtration. A mixing soy sauce is mixed 40% of brewed soy sauce and 45% of soybean acid-decomposed soy sauce with 15% of the silk amino acid solution, and aged at 90deg.C for 24hours.
Abstract:
PURPOSE: A method for preparing a kind of ice cream made from an aqueous solution of silk amino acid containing free amino acid and optionally milk fat is provided to serve high functional ice cream products promoting physical health of women and children. CONSTITUTION: The method includes the following steps: (i) removing sericin from silk fibroin protein; (ii) getting an aqueous solution of silk amino acid of pH 7 by adjusting concentration of the protein to 10 by adding 0.5N hydrochloric acid to the silk fibroin protein, hydrolyzing at 121deg.C for 4hr and adding 9M of sodium hydroxide to the protein; (iii) decolorizing and deodorizing the aqueous solution and passing the solution through an electric dialyzing device or a gel filtering device; (iv) mixing 0.5-2wt% of condensed solution of apple, 23-35wt% of raw cream, 9-12wt% of powdered nonfat milk, 5-13wt% of sugar, 4-7wt% of starch syrup, 0.2-0.6wt% of emulsion stabilizer, 0-2wt% of hydrous crystalline dextrose, 0-7wt% of oligosaccharide, 0-0.2wt% of spice, and refined water at 60-65deg.C for 20-40min; (iv) homogenizing and sterilizing the mixture at 85-90deg.C for 20-30sec before cooling; and then (v) adding 2-6wt% of the aqueous solution(18brix) to the mixture and aging at 2-6deg.C for 4-12 hr before freezing and increasing the quantity of the mixture of ice cream to a rate of 50-150%. The ice cream not containing milk fat comprises 5-2wt% of condensed solution of apple, 9-12% of coconut hardened oil, 9-12wt% of powdered nonfat milk, 5-13wt% of sugar, 4-7wt% of starch syrup, 0.2-0.6wt% of emulsion stabilizer, 0-2wt% of hydrous crystalline dextrose, 0-7wt% of oligosaccharide, 0-0.2wt% of spice, and refined water. The emulsion stabilizer includes 22.5% of guar gum, 19.5% of locust bean gum, 4.5% of carrageenan, 3.5% of Tamarind gum and 50% of Glycerin fatty acid ester.
Abstract:
본 발명은 아스퍼질루스 오리자에(Aspergillus oryzae) 및 바실루수(Bacillus)변종으로부터 제조된 3종의 식용 효소(Flavourzyme, Sumizyme, Protamax)를 사용하여 이것에 의한 바이오 실크 세리신 펩타이드 용액 및 분말 제조방법에 관한 것이다. 기존에 많이 쓰이고 있는 세리신의 분리·추출법인 고온 고압법과 비교하였을 때 세리신의 추출효과가 우수하였다. 또한 세리신의 회수율에 있어서도 최고 30%이상의 효과를 나타내었다. 이것은 효소의 산화 방지 및 팽윤 촉진을 위해 N 2 가스를 처리함으로써 효소 활성의 극대화를 적절히 사용한 본 발명의 독창적 방법이라 생각한다. 또한 전 아미노산 조성의 차이에 있어서는 대조인 고온 고압법으로 제조한 세리신과 크 차이를 보이지 않았으나, 평균 분자량의 분포는 효소처리 세리신(MW:8,500-12,000)이 고온 고압 처리 세시신(MW:12,000-15,000)보다 약간 낮은 결과를 나타내었는데, 이것은 고온 고압에 의한 세리신 추출보다도 온화한 조건으로 처리되었음을 나타내며, 분자량이 작음으로 인해 보다 쉽게 용해될 수 있음을 간접적으로 시사한다고 할 수 있다. 본 발명은 효소를 이용하여 최적의 조건으로 실크 세리신 펩타이드를 추출하는 방법으로서 추출한 세리신의 용도 및 응용분야는 광범위하게 확대 될 것이고, 추출하는 방법에 있어서도 고온 고압 추출법과는 비교되는 독창적인 추출방법이다.