Abstract:
The present invention relates to a bean composition containing conjugated linoleic acid (CLA) and a method for producing the same. More specifically, the bean composition is produced in which linoleic acid is converted into CLA by adding microorganisms in a bean pulverized material so that the utilization of beans is maximized. Accordingly, the domestic production rate of beans is increased. CLA components are contained in biological activity functions of microorganisms which are beneficial for the human body so as to enhance efficiency in weight loss, cholesterol decrease, hyperlipidemia alleviation, and atherosclerosis alleviation. Accordingly, the bean composition can be utilized as functional food and a medical product.
Abstract:
PURPOSE: Molecular markers and a detecting method using the same are provided to identify sequences of three subunits of bean beta-conglycinin by using a molecular marker, and to easily detect existence of three subunits of beta-conglycinin by directly extracting DNA from plant without conducting SDS-PAGE. CONSTITUTION: Primer pairs for selecting bean cultivar which detects a subunit of bean beta-conglycinin is selected from a group consisting of; a primer pair represented by sequence number 1 and 2; a primer pair represented by sequence number 3 and 4; a primer pair represented by sequence number 5 and 6; a primer pair represented by sequence number 7 and 8; a primer pair represented by sequence number 9 and 10; a primer pair represented by sequence number 11 and 12; and a primer pair represented by sequence number 13 and 14. A method of selecting bean cultivar which detects a subunit of bean beta-conglycinin comprises; (i) a step which separates DNA from a bean sample; (ii) a step which amplifies a target sequence by performing an amplification reaction with at least one primer pairs among the primer pairs and the separated DNA as a template; and (iii) a step which detects the amplified products.
Abstract:
본 발명은 베타-콘글리시닌의 3-서브유닛을 판별하기 위한 분자표지 마커 및 이를 이용한 선별방법에 관한 것으로, 더욱 상세하게는 하나의 분자표지 마커를 이용하여 콩 단백질의 베타-콘글리시닌의 α,α',β 세가지 서브유닛에 대한 염기서열을 선별함으로써 콩 종자를 이용하여 단백질 전기영동(SDS-PAGE)을 할 필요 없이 식물체 잎에서 바로 DNA를 추출하여 베타-콘글리시닌의 3-서브유닛의 유무를 쉽게 알 수 있으며 분자표지를 이용하여 세대단축의 효과와 정확한 개체를 선발할 수 있어 많은 노동력과 비용절감의 효과가 있다. 뿐만 아니라 베타-콘글리시닌의 α와 α' 서브유닛이 제거된 유전자원을 이용하여 국내 우수품종과 교배한 개체는 수입콩과의 차별화를 이룰 수 있는 효과가 있는 베타-콘글리시닌의 3-서브유닛을 판별하기 위한 분자표지 마커 및 이를 이용한 선별방법에 관한 것이다.