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公开(公告)号：KR1020140057786A
公开(公告)日：2014-05-14
申请号：KR1020120123971
申请日：2012-11-05
Applicant: 대한민국(농촌진흥청장)
IPC: C12N15/85 , C12N15/12 , A01K67/027
Abstract: The present invention relates to a transgenic silkworm that produces red fluorescent silk. More specifically, a recombinant expression vector which includes a gene construct in which a marker gene regulatory promoter, a marker gene, a silkworm-derived fibroin promoter, and an anemone-derived red fluorescent gene are operatively linked and a silkworm is transformed by the recombinant expression vector so that a transgenic silkworm that produces red fluorescent silk can be produced. The red fluorescent silk that the transgenic silkworm produces represents thin natural red color even in natural light so that a separated coloring process is not needed and the silk is environment-friendly and economical. When a light beam of a particular wavelength is irradiated, the silk represents bright red fluorescent color in the darkness so as to be usefully used as material for high value clothes and fashion or wallpaper compared with general silk.
Abstract translation: 本发明涉及产生红色荧光丝的转基因蚕。 更具体而言，包含基因构建体的重组表达载体，其中标记基因调节启动子，标记基因，蚕衍生的丝心蛋白启动子和来自海葵的红色荧光基因可操作地连接，并且通过重组体转化蚕 表达载体，从而可以产生产生红色荧光丝的转基因蚕。 转基因蚕产生的红色荧光丝即使在自然光下也能表现出稀薄的天然红色，因此不需要分离的着色过程，丝绸环保，经济。 当照射特定波长的光束时，丝绸在黑暗中代表明亮的红色荧光颜色，以便与普通丝绸相比有用地用作高价值衣服和时尚或壁纸的材料。
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公开(公告)号：KR1020130141958A
公开(公告)日：2013-12-27
申请号：KR1020120065118
申请日：2012-06-18
Applicant: 대한민국(농촌진흥청장)
Abstract: The present invention relates to a transgenic silkworm having hSCF recombinant protein. A method for preparing the transgenic silkworm having hSCF recombinant protein of the present invention comprises the steps of: introducing a dHsp70 promoter and a 3xP3 promoter to a transfer vector in order to prepare an expression vector for silkworm transformation; and micro-injecting the expression vector for the silkworm transformation into a silkworm egg and preparing a transduced transgenic silkworm. By the present invention, the transgenic silkworm, which is capable of mass producing stem cell factors (hSCF) which are useful to the human body at low costs, is provided. [Reference numerals] (1) Not transformed silkworm larva after 5 days;(2) Transformed silkworm larva after 5 days
Abstract translation: 本发明涉及具有hSCF重组蛋白的转基因蚕。 制备本发明具有hSCF重组蛋白的转基因蚕的方法包括以下步骤：将dHsp70启动子和3xP3启动子引入转移载体，以制备用于蚕转化的表达载体; 并将蚕转化的表达载体微注射到蚕卵中并制备转导的转基因蚕。 通过本发明，提供了能够以低成本大量生产对人体有用的干细胞因子（hSCF）的转基因蚕。 [参考数字]（1）5天后未转化的蚕幼虫;（2）5天后转化的蚕幼虫
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63.发明公开호랑나비 유충에서 분리한 항균펩타이드 파필리오신2의 유전자, 이를 이용한 재조합 항균펩타이드 파필리오신2 및 이의 대량생산방법 无效抗生素PEPTIDE PAPILIOCIN2的基因分离自黑曲霉BARTERFLY的LARVAE，使用其的重组抗生素肽PAPILIOCIN2及其生产的大量方法
公开(公告)号：KR1020130007783A
公开(公告)日：2013-01-21
申请号：KR1020110068317
申请日：2011-07-11
Applicant: 대한민국(농촌진흥청장)
Abstract: PURPOSE: A papiliocin 2 gene isolated from a Papilio xuthus larva, an antipeptide using the same, and a method for producing a large amount of recombinant papiliocin 2 are provided to ensure antibacterial activity against gram-negative bacteria, Candida albicans, and resistant bacteria(MEREC). CONSTITUTION: A gene of antibacterial peptide papiliocin 2 is denoted by sequence number 7 and is isolated from Papilio xuthus larva. A recombinant antibacterial peptide papiliocin 2 is denoted by sequence number 9. A recombinant expression vector, pET32-Papiliocin2 contains the recombinant antibacterial peptide papiliocin 2. A recombinant E.col BL21(DE3)-(pET32-Papiliocin2) is formed by transforming with the recombinant expression vector pET32-Papiliocin2. A method for producing a large amount of the recombinant antibacterial peptide pailiocin 2 comprises: a step of preparing the recombinant antibacterial peptide papiliocin 2(sequence number 9); a step of preparing the recombinant E.coli by the recombinant expression vector containing the recombinant antibacterial peptide papiliocin 2; and a step of culturing the recombinant E.coli and isolating and purifying a water soluble recombinant fusion protein.
Abstract translation: 目的：提供从Papilio xuthus幼虫分离的乳糜蛋白酶2基因，使用其的抗肽，以及用于产生大量重组体生物素酶2的方法，以确保对革兰氏阴性细菌，白色念珠菌和抗性细菌的抗菌活性（ MEREC）。 构成：抗菌肽乳木球蛋白2的基因由序列号7表示，并从Papilio xuthus幼虫中分离。 重组抗菌肽乳木球蛋白2以序列号9表示。重组表达载体pET32-青霉素2含有重组抗菌肽乳糖酶2.重组大肠杆菌BL21（DE3） - （pET32-青霉素2）通过用 重组表达载体pET32-Papiliocin2。 一种生产大量重组抗菌肽潘立替康2的方法，包括：制备重组抗菌肽乳链球蛋白2（序列号9）的步骤; 通过重组表达载体制备重组大肠杆菌的步骤，该重组表达载体含有重组抗菌肽乳木球蛋白2; 以及培养重组大肠杆菌并分离和纯化水溶性重组融合蛋白的步骤。
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64.发明公开누에 형질전환용 열충격단백질 70 프로모터, 이의 증폭용 프라이머, 이 프로모터를 포함하는 발현벡터, 이 발현벡터로 형질전환된 누에 형질전환체 및 그 제조방법 有权热冲击蛋白70促进剂用于丝光纤变换，用于放大的引导器，包含促销器的表达向量，用表达式向量变换的丝绸变换器及其生产方法
公开(公告)号：KR1020130006151A
公开(公告)日：2013-01-16
申请号：KR1020110068021
申请日：2011-07-08
Applicant: 대한민국(농촌진흥청장)
Abstract: PURPOSE: A HSP70 promoter for transforming silkworm, a primer for amplifying the same, and an expression vector containing the promoter are provided to express green fluorescent genes in silkworm eggs and larvae, and to early determine transformation. CONSTITUTION: A HSP70 promoter for transforming silkworm is denoted by sequence number 1, and is isolated in a silkworm. A primer for amplifying the promoter has sequences of sequence numbers 2 and 3. An expression vector for transforming silkworm contains the HSP70 promoter. A method for preparing a silkworm transformant comprises: a step of treating a dormant silkworm egg with hydrochloric acid at 25 deg. C for 1 hour; a step of transducing green fluorescent gene at downstream of the HSP70 promoter(sequence number 1) and cloning fused vector to prepare the expression vector; and a step of microinjecting the expression vector to the silkworm egg.
Abstract translation: 目的：提供用于转化蚕的HSP70启动子，其扩增引物和含有启动子的表达载体，以在蚕卵和幼虫中表达绿色荧光基因，并早期确定转化。 构成：用于转化蚕的HSP70启动子由序列号1表示，并在蚕中分离。 用于扩增启动子的引物具有序列号2和3的序列。转化蚕的表达载体含有HSP70启动子。 制备蚕转化体的方法包括：用25℃的盐酸处理休眠蚕卵的步骤。 C 1小时; 在HSP70启动子（序列号1）的下游转染绿色荧光基因并克隆融合载体以制备表达载体的步骤; 以及向蚕卵显微注射表达载体的步骤。
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65.发明公开녹색 형광실크를 생산하기 위한 형질전환용 누에알, 그 누에알로부터 제조된 누에 형질전환체 및 이를 통해 생산된 형질전환 누에 有权用于生产绿色荧光丝的转基丝丝蛋白，使用其制造的丝素变压器和使用其生产的转基因丝
公开(公告)号：KR1020120124644A
公开(公告)日：2012-11-14
申请号：KR1020110042423
申请日：2011-05-04
Applicant: 대한민국(농촌진흥청장)
Abstract: PURPOSE: A silkworm egg for transformation for producing green fluorescence silk and a transformant from the same are provided to stably and cheaply produce useful materials. CONSTITUTION: A silkworm egg for transformation is prepared by acid treatment of an egg of dormant state at 25 Deg. C. for one hour. A method for preparing a silkworm transformant comprises: a step of preparing the silkworm egg; a step of cloning a cassette vector to a basic vector in which a red fluorescent gene is introduced at 3XP promoter downstream and preparing a delivery system for silkworm transformation; and a step of microinjection of the delivery system to the silkworm egg. [Reference numerals] (AA) Hatching rate(%); (BB, DD) Untreated; (CC, EE) Micro-injection
Abstract translation: 目的：提供用于生产绿色荧光丝的转化的蚕卵及其转化体，以稳定且廉价地生产有用的材料。 构成：通过酸处理休眠状态的鸡蛋25度来制备用于转化的蚕卵。 C.一个小时。 一种制备蚕转化体的方法，包括：制备蚕卵的步骤; 将盒载体克隆到其中在下游3XP启动子处引入红色荧光基因并制备用于蚕转化的递送系统的碱性载体的步骤; 以及向蚕卵显微注射输送系统的步骤。 （标号）（AA）孵化率（％） （BB，DD）未处理; （CC，EE）微量注射
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Patent Agency Ranking
公开(公告)号：KR101021226B1
公开(公告)日：2011-03-11
申请号：KR1020100037204
申请日：2010-04-22
Applicant: 대한민국(농촌진흥청장) , 대진대학교 산학협력단
CPC classification number: A61K38/08
Abstract: PURPOSE: A composition containing coprisin peptide derivative CopA3(HL) for treating Pseudomembranous colitis is provided to prevent antibiotics effect to helpful microbes such as Bifidobacterium and Lactobacillus. CONSTITUTION: A composition for treating Pseudomembranous colitis contains coprisin peptide derivative CopA3(HL) as an active ingredient. The coprisin peptide derivative CopA3(HL) has an amino acid sequence(L-L-C-I-A-L-R-K-K). The coprisin peptide derivative suppresses Clostrdium difficile in the large intestine. The coprisin peptide derivative CopA3(HL) is D-type.
Abstract translation: 目的：提供含有用于治疗假膜性结肠炎的复合蛋白肽衍生物CopA3（HL）的组合物，以防止抗生素对有益微生物如双歧杆菌和乳杆菌的作用。 构成：用于治疗假膜性结肠炎的组合物含有作为活性成分的共感染肽衍生物CopA3（HL）。 共聚蛋白肽衍生物CopA3（HL）具有氨基酸序列（L-L-C-I-A-L-R-K-K）。 共感染肽衍生物抑制大肠中的艰难梭菌（Clostrdium difficile）。 共聚蛋白肽衍生物CopA3（HL）是D型。
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67.发明公开흰점박이꽃무지유충으로부터 분리한 신규한 항진균펩타이드유전자 및 상기 항진균펩타이드 및 그 유도체 有权从BEETLE，PROTAETIA BREVITARSIS，LARVAE，SAID抗真菌肽及其模拟物分离的抗真菌肽基因
公开(公告)号：KR1020100050141A
公开(公告)日：2010-05-13
申请号：KR1020080109281
申请日：2008-11-05
Applicant: 대한민국(농촌진흥청장)
IPC: C07K14/435 , C07K7/00 , C07K7/02
Abstract: PURPOSE: A novel antifungal peptide gene isolated from Protaetia brevitarsis larva is provided to use as an agent for preventing and treating fungal diseases. CONSTITUTION: A novel antifungal peptide isolated from Protaetia brevitarsis larva comprises a base of sequence number 1. The novel antipeptide isolated from Protaetia brevitarsis larva has an amino acid sequence of sequence number 2. A novel antifungal peptide derivative isolated from Protaetia brevitarsis larva has an amino acid of sequence number 4(9Pbm1; ALWLAIGRG), 5(9Pbm2; ALWLAIGKG), 6(9Pbm3; RLWLAIGKG), 7(9Pbm4; RLLLAIGRG), 8(9Pbm5; RLWLRIGRG), or 9(9Pbm6; RLWLAIGRG).
Abstract translation: 目的：提供从革兰氏淡紫色幼虫中分离出的新型抗真菌肽基因，用作预防和治疗真菌病的药剂。 构成：从裂殖酵母中分离出的新型抗真菌肽包含序列号为1的碱基。从短尾from幼虫中分离出的新型抗肽具有序列号2的氨基酸序列。从裂殖酵母幼虫分离的新型抗真菌肽衍生物具有氨基 序列号4的酸（9Pbm1; ALWLAIGRG），5（9Pbm2; ALWLAIGKG），6（9Pbm3; RLWLAIGKG），7（9Pbm4; RLLLAIGRG），8（9Pbm5; RLWLRIGRG）或9（9Pbm6; RLWLAIGRG）。
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公开(公告)号：KR1020170031843A
公开(公告)日：2017-03-22
申请号：KR1020150129052
申请日：2015-09-11
Applicant: 대한민국(농촌진흥청장)
IPC: C12N15/85 , C12N15/89 , C07K14/435 , A01K67/033
Abstract: 본발명은누에형질전환기술을이용하여누에체액에서멜리틴항균펩타이드를생산하는것으로서, 본실험에서는누에유래의액틴3 프로모터를이용하여멜리틴항균펩타이드를발현시켰다. 누에형질전환체선발을위해서는 3xP3 프로모터와 EGFP 유전자를이용하여선발하였고, 300 개의누에알에미세주입하여 F1 세대에서 11 아구(bloods)의누에형질전환체를선발하였다. 선발된누에형질전환체는초기배단계의눈과신경조직, 유충과번데기그리고성충의눈에서 EGFP 형광단백질이발현되는것을확인할수 있었다. 또한 G2 세대누에형질전환체를 5령 5일유충까지사육후, 체액을채취한후 전처리하였다. 이시료를항균활성검정을하였고, 총 10마리의누에를선발할수 있었다. 이렇게선발된 누에는서로교배를통해서계대사육을하였다. 이러한과정으로선발된 G3세대누에형질전환체를이용하여앞의과정과동일한방법으로항균할성을검정하였다. 그결과대조군으로사용된시그마사의 melittin (0.016mg/ml)과거의동일한항균활성을나타내었다. 그럼으로이상의결과에서 melittin 항균펩타이드를생산하는누에형질전환체가성공적으로제작되었음을확인할수 있었다.
Abstract translation: 本发明通过使用转基因蚕技术生产家蚕体液中的抗微生物肽蜂毒肽，在该实验中，使用蚕衍生抗微生物肽蜂毒肽的3-肌动蛋白启动表达。 在F1代使用3XP3启动子和EGFP基因被选择转基因蚕与起始材料，选择了转基因蚕分区域11（血液）通过显微注射到300个蚕卵。 选择的转基因蚕可能是在早期阶段时间眼睛和神经组织，幼虫和蛹和成虫的眼睛证实EGFP荧光蛋白的表达。 将G2代家蚕转基因植物培养至5龄第5天幼虫，并收集体液并进行预处理。 测试该样品的抗微生物活性，并选择总共10只家蚕。 这些选择的家蚕通过杂交相互交叉。 以与上述相同的方式测试以这种方式选择的G3代家蚕转化体的抗微生物活性。 结果示为Sigma的蜂毒肽（0.016mg / ml）的用作对照过去相同的抗微生物活性。 由此证实，成功生产了产生蜂毒肽抗微生物肽的家蚕转化体。
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