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    구제역 Ｏ형 ３Ｂ 부분이 결손된 바이러스
    67.
    发明公开
    구제역 Ｏ형 ３Ｂ 부분이 결손된 바이러스 有权
    FOOT和MOUTH DISEASE VIRUS轴承3部分删除的O系列基因

    公开(公告)号：KR1020130114945A

    公开(公告)日：2013-10-21

    申请号：KR1020120037406

    申请日：2012-04-10

    Applicant: 대한민국(농림축산식품부 농림축산검역본부장)

    Inventor： 박종현 ,  김수미 ,  이광녕 ,  초가기 ,  이여주 ,  김은정 ,  방민우 ,  이서용 ,  고영준 ,  이향심 ,  조인수

    IPC: C12N15/63 ,  C12N15/33 ,  C12N7/01

    Abstract: PURPOSE: Foot and mouth disease virus bearing 3B partially deleted gene of serotype O is provided to weaken pathogenicity in an animal, thereby being safer than pathogenic virus when the virus is used for a disinfectant experiment or an antivirus experiment etc in a laboratory. CONSTITUTION: A plasmid has a base sequence like a sequence number 1. In the plasmid, a foot and mouth disease virus gene capable of producing foot and mouth disease virus bearing 3B partially deleted gene of serotype O is inserted. The plasmid is used for determining the foot and mouth disease virus. The foot and mouth disease virus bearing 3B partially deleted gene of serotype O is produced from the plasmid.

    Abstract translation: 目的：提供具有3B部分缺失的血清型O基因的口蹄疫病毒，以削弱动物的致病性，从而当病毒用于实验室中的消毒剂实验或抗病毒实验等时，比病原性病毒更安全。 构成：质粒具有序列号1的碱基序列。在质粒中插入能产生具有3B部分缺失的血清型O基因的口蹄疫病毒的口蹄疫病毒基因。 质粒用于测定口蹄疫病毒。 由质粒产生携带3B部分缺失的血清型O基因的口蹄疫病毒。

    구제역 Ｏ형, Ａ형, Ａｓｉａ１형 바이러스 진단용 프라이머 및 이를 이용한 멀티플렉스 ＲＴ－ＰＣＲ 방법
    68.
    发明公开
    구제역 Ｏ형, Ａ형, Ａｓｉａ１형 바이러스 진단용 프라이머 및 이를 이용한 멀티플렉스 ＲＴ－ＰＣＲ 방법 有权
    O，A和亚洲1种口蹄疫病毒和多发病毒多重RT-PCR方法的差异

    公开(公告)号：KR1020130014933A

    公开(公告)日：2013-02-12

    申请号：KR1020110076694

    申请日：2011-08-01

    Applicant: 대한민국(농림축산식품부 농림축산검역본부장)

    Inventor： 김수미 ,  르반빤 ,  박종현 ,  이광녕 ,  조인수

    IPC: C12N15/11 ,  C12Q1/68

    CPC classification number: C12Q1/6844 ,  C12Q1/701

    Abstract: PURPOSE: A primer for diagnosing foot-and-mouth disease type O, type A, and type Asia 1 viruses and a multiplex RT-PCR method using the same are provided to simultaneously diagnose three kinds of serotypes through one-step RT-PCR. CONSTITUTION: A primer set for diagnosing foot-and-mouth type O virus has VN-VP1R forward primer of sequence number 1 and VN-OF reverse primer of sequence number 2. A primer set for diagnosing foot-and-mouth disease type Asia 1 virus contains VN-VP1R forward primer of sequence number 1 and VN-As1F reverse primer of sequence number 3. A primer set for diagnosing foot-and-mouth type A virus contains VN-VP1R forward primer of sequence number 1 and AN-AF reverse primer of sequence number 4.

    Abstract translation: 目的：提供一种用于诊断口蹄疫O型，A型和亚型1型病毒的引物和使用其的多重RT-PCR方法，以通过一步RT-PCR同时诊断三种血清型。 构成：用于诊断口蹄型O型病毒的引物组具有序列号1的VN-VP1R正向引物和序列号2的VN-OF反向引物。用于诊断口蹄疫亚型1的引物组 病毒含有序列号1的VN-VP1R正向引物和序列号3的VN-As1F反向引物。用于诊断口蹄疫型A病毒的引物组含有序列号1的VN-VP1R正向引物和AN-AF反向 序列号4的引物。

    구제역 7가지 혈청형 감별을 위한 유전자 진단키트
    69.
    发明公开
    구제역 7가지 혈청형 감별을 위한 유전자 진단키트 有权
    用于分化病毒和病毒病毒七代的RT-PCR

    公开(公告)号：KR1020110038380A

    公开(公告)日：2011-04-14

    申请号：KR1020090095646

    申请日：2009-10-08

    Applicant: 대한민국(농림축산식품부 농림축산검역본부장)

    Inventor： 김수미 ,  이광녕 ,  박종현 ,  고영준 ,  이향심 ,  권창희

    IPC: C12Q1/68 ,  C12N15/11

    CPC classification number: H04W8/20 ,  C12Q1/6844 ,  C12N15/11

    Abstract: PURPOSE: A method for diagnosing gene for distinguishing seven kinds of serotype of foot-and-mouth disease is provided to ensure fastness and convenience. CONSTITUTION: A method for diagnosing gene for distinguishing seven kinds of serotype of foot-and-mouth comprises a step of diagnosing seven type of serotype(O, A, Asia 1, C, SAT 1, SAT 2, and SAT 3) by different band size. The diagnosis method comprises: a step of diagnosing type O and Asia 1, type A and C, and type SAT 1, SAT 2, and SAT 3. SAT 1, SAT 2 and SAT 3 has common band size. A primer for amplifying type O has a base sequence of sequence numbers 1, 2, 3, and 5.

    Abstract translation: 目的：提供一种诊断口蹄疫七种血清型鉴定基因的方法，以确保牢固性和便利性。 构成：用于区分七种口蹄血清型的基因的诊断方法包括通过不同的方式诊断七种血清型（O，A，亚洲1，C，SAT 1，SAT 2和SAT 3）的步骤 带尺寸。 诊断方法包括：诊断类型O和亚洲1，类型A和C，以及类型SAT 1，SAT 2和SAT 3的步骤.SAT 1，SAT 2和SAT 3具有共同的带尺寸。 用于扩增型O的引物具有序列号1,2,3和5的碱基序列。

    구제역바이러스 아시아 １형 항체를 검출하기 위한 진단방법
    70.
    发明公开
    구제역바이러스 아시아 １형 항체를 검출하기 위한 진단방법 有权
    用于检测禽流感病毒类型的病毒的诊断方法1

    公开(公告)号：KR1020110031012A

    公开(公告)日：2011-03-24

    申请号：KR1020090088714

    申请日：2009-09-18

    Applicant: 대한민국(농림축산식품부 농림축산검역본부장) ,  주식회사 메디안디노스틱

    Inventor： 고영준 ,  이향심 ,  정혜영 ,  허은정 ,  박종현 ,  이광녕 ,  김수미 ,  권창희 ,  주후돈 ,  장병식

    IPC: G01N33/68 ,  C12N15/09 ,  G01N33/563

    Abstract: PURPOSE: A diagnosis method for detecting food-and-mouth disease virus type asia 1 antibody is provided to quickly measure vaccine antibody titer. CONSTITUTION: An antibody for food-and-mouth disease virus type asia 1 is detected using a gene recombinant protein antigen of food-and-mouth disease virus type asia 1 and food-and-mouth disease virus type asia 1-specific monoclonal antibody. A diagnosis method for detecting food-and-mouth disease virus type asia 1 antibody comprises: a step of reacting serotype common antibody 70-17 of food-and-mouth disease virus type asia 1, type C, O, and A in 96-well microplate; a step of washing monoclonal antibodies which are not conjugated on the plate to remove; a step of reacting with a test serum sample; a step of washing test serum sample which is not conjugated; a step of reacting horseradish peroxidase-conjugated type asia 1-specific monoclonal antibody; a step washing monoclonal antibodies which is not conjugated to the plate; and a step of measuring absorbance.

    Abstract translation: 目的：提供一种用于检测食源性病毒亚型1抗体的诊断方法，以快速测定疫苗抗体效价。 构成：使用食品口蹄疫病毒亚型1和口蹄疫病毒型亚洲1型单克隆抗体的基因重组蛋白抗原检测食品口蹄疫病毒亚型1的抗体。 一种用于检测食源病病毒亚型1抗体的诊断方法包括：将食物口蹄疫病毒亚型1（C，O和A型）的血清型共同抗体70-17与96- 微孔板 洗涤未在板上结合以除去的单克隆抗体的步骤; 与测试血清样品反应的步骤; 洗涤不结合的血清样品的步骤; 辣根过氧化物酶偶联型亚洲1型单克隆抗体的反应步骤; 步骤洗涤未与板结合的单克隆抗体; 和测量吸光度的步骤。

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