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公开(公告)号：KR1020150130748A
公开(公告)日：2015-11-24
申请号：KR1020140057724
申请日：2014-05-14
Applicant: 대한민국(농촌진흥청장)
IPC: A61K36/899 , C12N15/63 , A61P35/00
CPC classification number: A61K36/899 , A23L5/00 , A23L33/10 , A61J3/02 , A61J3/07 , A61K36/18 , A61K36/88
Abstract: 본발명은토코트리에놀생합성형질전환벼를포함하는전립선암예방또는치료용조성물에관한것으로서, 보다상세하게는 HGGT(homogentisic acid geranylgeranyl transferase) 유전자가형질전환되어토코트리에놀을생합성할수 있는형질전환벼의추출물을유효성분으로포함하는, 전립선암예방또는치료용약학조성물, 및전립선암예방또는개선용식품조성물에관한것이다. 본발명의 HGGT 유전자가형질전환된흑남벼의추출물은전립선암의예방및 치료효능을가짐으로써, 전립선암의치료를위한의약품및 건강기능식품등에적용되어널리활용될수 있다.
Abstract translation: 本发明涉及用于预防或治疗前列腺癌的组合物，其包含能够合成生育三烯酚的转化水稻，更具体地，涉及用于预防或治疗前列腺癌的药物组合物和用于预防或减轻前列腺癌的食物组合物，其包含提取物 的转化水稻，其能够通过转化作为有效成分的同基酸香叶基香叶基转移酶（HGGT）基因合成生育三烯酚。 HGGT基因转化水稻提取物 本发明的Heugnambyeo在预防和治疗前列腺癌中是有效的，因此被广泛用于治疗前列腺癌的药物，保健功能食品等中。
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Patent Agency Ranking
公开(公告)号：KR1020150057084A
公开(公告)日：2015-05-28
申请号：KR1020130139950
申请日：2013-11-18
Applicant: 대한민국(농촌진흥청장)
CPC classification number: A23L33/185 , A23L33/105 , A23L19/00 , A23L11/32 , A23L33/10
Abstract: 본발명은옥수수수염추출물및 그제조방법에관한것으로, 보다상세하게는옥수수수염에존재하는생체내항산화작용을증가시키고항산화스트레스에의해유발된신경세포사멸억제효과를가지는메이신의추출을극대화하는동시에항산화작용을향상시키는다른유효물질들을부가적으로추출할수 있고, 상기유효물질들의추출과정에서종래의열수추출방법에의해추출된경우에비해추출되는유효물질의파괴, 변형을최소화할수 있는옥수수수염유래메이신을포함하는유효물질의고수율추출방법에관한것이다.
Abstract translation: 本发明涉及一种玉米丝提取物及其制备方法。 更具体地说，提供高产量的含有玉米丝来源的麦芽糖的活性物质的方法，其能够使存在于玉米丝中的马斯辛的提取最大化，增加了活体中的抗氧化活性，并且具有引起的神经元抗细胞凋亡作用 通过抗氧化应激，同时提取提高抗氧化活性的其他附加活性物质，并与现有的水热提取方法相比，最大限度地减少活性物质提取活性物质的破坏和转化。
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公开(公告)号：KR1020140039844A
公开(公告)日：2014-04-02
申请号：KR1020120106595
申请日：2012-09-25
Applicant: 대한민국(농촌진흥청장)
CPC classification number: C12Q1/6888 , C12Q2600/158
Abstract: The present invention relates to a primer set for discriminating soybean cultivars and a method for discriminating soybean cultivars using the same. More specifically, the present invention uses an STS-CAPS marker to discriminate the cultivars, and therefore, can discriminate 110 species of the soybean cultivars by using an agarose gel to be used for a quality control and a quality assurance of the soybean.
Abstract translation: 本发明涉及用于鉴别大豆品种的引物组和用于鉴别大豆品种的方法。 更具体地说，本发明使用STS-CAPS标记来区分品种，因此可以通过使用用于质量控制和大豆质量保证的琼脂糖凝胶来鉴别110种大豆品种。
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公开(公告)号：KR1020140026725A
公开(公告)日：2014-03-06
申请号：KR1020120092147
申请日：2012-08-23
Applicant: 대한민국(농촌진흥청장)
IPC: C07K1/16 , C07K14/415
CPC classification number: A23L33/105 , A23L11/05 , A23L11/07
Abstract: The present invention relates to a method of separating and purifying isoflavone and soyasaponin from soybean embryos, and more specifically, to the method of separating and purifying isoflavone and soyasaponin from soybean embryos in which a processing time is reduced by separating extracts containing embryos separated from soybeans through preparative chromatography, thereby separating the isoflavone, which is a bioactive substance, and saponin at the same time; has excellent workability; is capable of purifying the isoflavone and saponin having high chemical purity; and can recycle soybean byproducts that are treated as waste, thereby reducing environmental pollution. [Reference numerals] (AA) Dry raw beans (40°C, 72 hours); (BB) Separate soybean embryos (sifter with 2.36, 2.0, 1.18 mm); (CC) Remove fat of hexane (room temperature, 48 hours); (DD) Extract methanol (90°C, reflux cooling extraction 6 hours, twice); (EE) Condense and remove protein and sugar (twice); (FF) Separate by a C_18 (125�, 55-105쨉m) column catridge; (GG) Divide isoflavone (ISF-1); (HH,LL) Freeze-dry; (II) Re-separate ISF-1 (gel permeation column); (JJ) Check characteristics of isoflavone (ISF-1-1. ISF-1-2); (KK) Divide four kinds of soyasaponin; (MM) Check a molecular weight of the soyasaponin (Q-TOF) (SP-1,SP-2,SP-3,SP-4)
Abstract translation: 本发明涉及从大豆胚胎中分离和纯化异黄酮和大豆皂角苷的方法，更具体地说，涉及从大豆胚分离和纯化异黄酮和大豆皂角苷的方法，其中通过分离含有从大豆分离的胚胎的提取物来减少处理时间 通过制备色谱法，同时分离作为生物活性物质的异黄酮和皂苷; 具有优异的可加工性; 能够纯化具有高化学纯度的异黄酮和皂苷; 并可回收大量副产品，作为废物处理，从而减少环境污染。 （AA）干粗豆（40℃，72小时）; （BB）分离大豆胚胎（筛分为2.36,2.0,1.18mm）; （CC）除去己烷中的脂肪（室温48小时）; （DD）萃取甲醇（90℃，回流冷却提取6小时，两次）; （EE）冷凝并去除蛋白质和糖（两次）; （FF）由C_18（125 ，55-105쨉m）色谱柱分开; （GG）除异黄酮（ISF-1）; （HH，LL）冷冻干燥; （II）重新分离ISF-1（凝胶渗透柱）; （JJ）检查异黄酮的特征（ISF-1-1。ISF-1-2）; （KK）分四种大豆皂角苷; （MM）检查大豆皂苷（Q-TOF）（SP-1，SP-2，SP-3，SP-4）的分子量
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公开(公告)号：KR1020130118594A
公开(公告)日：2013-10-30
申请号：KR1020120041585
申请日：2012-04-20
Applicant: 대한민국(농촌진흥청장)
CPC classification number: A23L33/105 , A23V2002/00 , A23V2200/02 , A23V2200/308 , A23V2200/3262 , A23V2300/14 , A61K36/48 , A61K2236/35 , A61K2236/37
Abstract: PURPOSE: An optimum extraction method of a tocochromanol component from beans is provided to obtain the high content of tocochromanol from the beans. CONSTITUTION: An extraction method of a tocochromanol component from beans comprises a step of extracting tocochromanol from the beans using an ethanol solvent distillation method. The beans are bean seeds or germinated beans. The bean seeds or germinated beans are crushed under the presence of liquid nitrogen. The bean seeds are extracted by using a mixed solution of 80-90 wt% of hexane and 10-20 wt% of ethyl acetate at 140-180°C through a Soxhlet method. The germinated beans are extracted by using an ethanol solution containing pyrogallol through a solvent extraction method. The germinated beans are germinated at a low temperature for 2-4 days. [Reference numerals] (AA) Add EtOAc10%; (BB) Add EtOAc15%; (CC) Add EtOAc20%
Abstract translation: 目的：从豆类中提取最佳的脱咖啡因成分提取方法，从豆中获得高含量的脱咖啡醇。 构成：来自豆类的脱咖啡醇组分的提取方法包括使用乙醇溶剂蒸馏法从豆中提取去苯乙醇醇的步骤。 豆类是豆类或发芽豆类。 豆子或发芽豆在液氮存在下粉碎。 通过使用80-90重量％的己烷和10-20重量％的乙酸乙酯的混合溶液在140-180℃下通过索格利特（Soxhlet）法提取豆子。 通过溶剂萃取方法使用含有连苯三酚的乙醇溶液提取发芽的豆。 发芽豆在低温下发芽2-4天。 （AA）加入EtOAc10％; （BB）加入EtOAc15％; （CC）加入EtOAc20％
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公开(公告)号：KR101303741B1
公开(公告)日：2013-09-09
申请号：KR1020100134339
申请日：2010-12-24
Applicant: 대한민국(농촌진흥청장)
Abstract: 본 발명은 토코트리에놀 생합성 과정에 관여하는 효소를 암호화하는 폴리뉴클레오티드를 포함하는 재조합 발현 벡터를 이용한 형질전환 콩을 발아시킨 발아콩 및 이의 제조방법에 관한 것이다.
본 발명의 재조합 벡터는 토코트리에놀을 합성하지 않는 콩에서 토코트리에놀 합성에 관련하는 효소를 안정적으로 발현시킴으로써 토코트리에놀 합성을 가능하게 하고 또한 8개 이성체를 모두 가진 비타민E 함량이 매우 높은 콩을 제조할 수 있도록 하며, 특히 상기 형질전환된 콩을 일정 조건에서 발아시킨 경우 항산화 활성을 크게 증가시킬 수 있다.
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