公开(公告)号：US11724256B2

公开(公告)日：2023-08-15

申请号：US16890417

申请日：2020-06-02

Applicant: Bio-Rad Laboratories, Inc

Inventor： Kalyan Handique

IPC: B01L3/00 ,  G01N15/14 ,  G01N1/40 ,  C12M1/00 ,  C12Q1/6888 ,  C12Q1/6813 ,  G01N15/10

CPC classification number: B01L3/502715 ,  B01L3/502746 ,  B01L3/502761 ,  C12M47/04 ,  C12Q1/6813 ,  C12Q1/6888 ,  G01N1/405 ,  G01N1/4077 ,  G01N15/1484 ,  B01L2200/0652 ,  B01L2200/0668 ,  B01L2300/0816 ,  B01L2300/0829 ,  G01N2015/1006 ,  G01N2015/149

Abstract: A system and method for automated single cell capture and processing is described, where the system includes a deck supporting and positioning a set of sample processing elements (including an integrated imaging subsystem); a gantry for actuating tools for interactions with the set of sample processing elements supported by the deck; and a base supporting various processing subsystems and a control subsystems in communication with the processing subsystems. The system can automatically execute workflows associated with single cell processing, including antibody detection, other protein detection, mRNA detection, and/or other applications associated with spatial transcriptomics.

公开(公告)号：US20230159917A1

公开(公告)日：2023-05-25

申请号：US18094278

申请日：2023-01-06

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Kalyan Handique ,  Austin Payne ,  Sida Wang ,  Patrick Michael Tegels ,  William T. Koederitz ,  Daniel Genord ,  Grey Parker ,  Brian Boniface ,  Katlyn Curtin Mehne ,  Alec William Hitchiner

IPC: C12N15/10 ,  B01L3/00 ,  B01L7/00 ,  G01F23/292 ,  C12N1/06 ,  B01L9/02 ,  G01N35/00 ,  C12Q1/6844

CPC classification number: C12N15/1096 ,  B01L3/502715 ,  B01L3/502738 ,  B01L3/502761 ,  B01L7/52 ,  C12N15/1093 ,  G01F23/292 ,  C12N1/06 ,  B01L3/502 ,  B01L3/52 ,  B01L9/02 ,  G01N35/00 ,  C12Q1/6844 ,  B01L2400/06 ,  B01L2300/123 ,  B01L2200/0647 ,  B01L2300/041 ,  B01L2300/0829 ,  B01L2300/0819 ,  B01L2300/1805 ,  B01L2300/047 ,  B01L2200/16 ,  B01L2300/06 ,  B01L2300/042 ,  B01L2300/0627 ,  B01L2300/0893 ,  B01L2300/1894 ,  B01L2400/043 ,  B01L2400/0633 ,  B01L2300/16

Abstract: A system and method for automated single cell capture and processing is described, where the system includes a deck supporting and positioning a set of sample processing elements; a gantry for actuating tools for interactions with the set of sample processing elements supported by the deck; and a base supporting various processing subsystems and a control subsystems in communication with the processing subsystems. The system can automatically execute workflows associated with single cell processing, including mRNA capture, cDNA synthesis, protein-associated assays, and library preparation, for next generation sequencing.

公开(公告)号：US20230056356A1

公开(公告)日：2023-02-23

申请号：US18048512

申请日：2022-10-21

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Kalyan Handique ,  John Stark ,  Yadwinder Deol ,  Vishal Sharma

IPC: C12Q1/6811 ,  C12Q1/6874 ,  C12N15/10

Abstract: A system and method for target material capture, the method comprising: receiving a set of target cells into an array of wells defined at a surface plane of a substrate; receiving a set of particles into the array of wells, thereby co-capturing the set of target cells and the set of particles; achieving a desired state for the array of wells upon receiving a washing fluid into a cavity in communication with the array of wells; receiving a lysis buffer into the cavity; receiving a partitioning fluid into the cavity, thereby displacing the lysis buffer from the cavity and partitioning each of the array of wells from adjacent wells, at the surface plane; and retaining intercellular material of the set of target cells, individually with the set of particles within the array of wells.

公开(公告)号：US20230016193A1

公开(公告)日：2023-01-19

申请号：US17951461

申请日：2022-09-23

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Kalyan Handique

IPC: G01N15/14 ,  C12M1/00 ,  B01L3/00 ,  B01L3/02 ,  G01N1/28 ,  G01N1/40 ,  G01N1/20

Abstract: A cell capture system including an array, an inlet manifold, and an outlet manifold. The array includes a plurality of parallel pores, each pore including a chamber and a pore channel, an inlet channel fluidly connected to the chambers of the pores; an outlet channel fluidly connected to the pore channels of the pores. The inlet manifold is fluidly connected to the inlet channel, and the outlet channel is fluidly connected to the outlet channel. A cell removal tool is also disclosed, wherein the cell removal tool is configured to remove a captured cell from a pore chamber.

公开(公告)号：US11504714B2

公开(公告)日：2022-11-22

申请号：US16790583

申请日：2020-02-13

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Kalyan Handique ,  Vishal Sharma ,  Priyadarshini Gogoi ,  William Chow ,  Austin Payne ,  Kyle Gleason ,  Brian Boniface ,  John Connolly ,  Sam Tuck

IPC: G01N33/543 ,  B01L3/00 ,  C12M1/00 ,  G01N33/574 ,  C12Q1/6816 ,  G01N33/569 ,  G01N15/14 ,  C12Q1/6886 ,  G01N15/10

Abstract: A system and method for isolating and analyzing single cells, wherein the system includes: an array of wells defined at a substrate, each well including an open surface and a well cavity configured to capture cells in one of a single-cell format and single-cluster format, and a fluid delivery module including a fluid reservoir superior to the array of wells through which fluid flow is controlled along a fluid path in a direction parallel to the broad face of the substrate; and wherein the method includes: capturing a population of non-cell particles into the array of wells in single-particle format; releasing, from the non-cell particles, a set of probes into the array of wells; capturing a population of cells into the array of wells in single-cell format; releasing biomolecules from each captured cell into the array of wells; and generating a set of genetic complexes comprising the biomolecules associated with a single captured cell and a subset of probes within individual wells of the array of wells.

公开(公告)号：US20220291190A1

公开(公告)日：2022-09-15

申请号：US17830325

申请日：2022-06-01

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Kalyan Handique ,  Kyle Gleason ,  Christopher Siemer ,  Priyadarshini Gogoi ,  Saedeh Sepehri Javdani ,  Gene Parunak ,  Aaron Kehrer ,  Jon Meines

IPC: G01N33/483 ,  G02B21/26 ,  G02B21/06 ,  G01N21/25 ,  G01N21/76 ,  G01N33/487 ,  G01N21/64

Abstract: A system for imaging captured cells comprising: an illumination module configured to illuminate a target object; a platform configured to position the target object in relation to the illumination module; a filter module configured to filter light transmitted to the target object and/or to filter light received from the target object, an optical sensor configured to receive light from the target object and to generate image data; and a focusing and optics module configured to manipulate light transmitted to the optical sensor. The system can further comprise one or more of: a control system configured to control at least one of the illumination module, the platform, the focusing and optics module, the filter module, and the optical sensor; a tag identifying system configured to identify and communicate tag information from system elements; a thermal control module configured to adjust temperature parameters of the system; and an image stabilization module.

公开(公告)号：US20220250075A1

公开(公告)日：2022-08-11

申请号：US17729760

申请日：2022-04-26

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Kalyan Handique ,  Priyadarshini Gogoi ,  Saedeh Javdani Sepehri ,  Kyle Gleason

IPC: B01L3/00 ,  G01N15/10 ,  G01N15/14 ,  C12M1/00 ,  C12Q1/6834 ,  C12Q1/6841 ,  G01N33/574

Abstract: A system and method for isolating and analyzing single cells, comprising: a substrate having a broad surface; a set of wells defined at the broad surface of the substrate, and a set of channels, defined by the wall, that fluidly couple each well to at least one adjacent well in the set of wells; and fluid delivery module defining an inlet and comprising a plate, removably coupled to the substrate, the plate defining a recessed region fluidly connected to the inlet and facing the broad surface of the substrate, the fluid delivery module comprising a cell capture mode.

公开(公告)号：US20220118442A1

公开(公告)日：2022-04-21

申请号：US17499529

申请日：2021-10-12

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Kalyan Handique ,  Ronald Lebofsky

IPC: B01L3/00 ,  C12Q1/686 ,  B01L7/00

Abstract: The invention(s) cover systems and methods for target detection in a multiplexed and rapid manner. Embodiments of the system can include: a base substrate; and an array of sample processing regions defined at a broad surface of the base substrate, wherein each of the array of sample processing regions includes: a set of microwell subarrays arranged in a gradient by volumetric capacity between an upstream end and a downstream end of each respective sample processing region, and a boundary separating each respective sample processing region from adjacent sample processing regions. The system can support methods, with example implementation by an automated platform, for returning preliminary results from a subset of microwells of the samples processing regions, as well as results pertaining to specific and non-specific amplification, for multiple targets of a sample.

公开(公告)号：US11275015B2

公开(公告)日：2022-03-15

申请号：US16732556

申请日：2020-01-02

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Kalyan Handique ,  Austin Payne ,  Vishal Sharma ,  Kyle Gleason ,  Priyadarshini Gogoi ,  Karthik Ganesan ,  Brian Boniface ,  Will Chow

IPC: G01N15/14 ,  G01N1/20 ,  G01N1/40 ,  G01N1/28 ,  B01L3/00 ,  G01N35/10 ,  G01N35/00 ,  G01N15/00 ,  G01N15/10 ,  B01L3/02 ,  G06K9/20 ,  G06K9/00 ,  C12M1/00

Abstract: A system and method for isolating and analyzing single cells, including: a substrate having a broad surface; a set of wells defined at the broad surface of the substrate, and a set of channels, defined by the wall, that fluidly couple each well to at least one adjacent well in the set of wells; and fluid delivery module defining an inlet and comprising a plate, removably coupled to the substrate, the plate defining a recessed region fluidly connected to the inlet and facing the broad surface of the substrate, the fluid delivery module comprising a cell capture mode.

公开(公告)号：US20210318226A1

公开(公告)日：2021-10-14

申请号：US17357287

申请日：2021-06-24

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Kalyan Handique

IPC: G01N15/14 ,  C12M1/00 ,  B01L3/00 ,  B01L3/02 ,  G01N1/28 ,  G01N1/40 ,  G01N1/20

Abstract: A cell capture system including an array, an inlet manifold, and an outlet manifold. The array includes a plurality of parallel pores, each pore including a chamber and a pore channel, an inlet channel fluidly connected to the chambers of the pores; an outlet channel fluidly connected to the pore channels of the pores. The inlet manifold is fluidly connected to the inlet channel, and the outlet channel is fluidly connected to the outlet channel. A cell removal tool is also disclosed, wherein the cell removal tool is configured to remove a captured cell from a pore chamber.