公开(公告)号：AU4649497A

公开(公告)日：1998-04-17

申请号：AU4649497

申请日：1997-09-19

Applicant: APPLIED SPECTRAL IMAGING LTD

Inventor： CABIB DARIO ,  BUCKWALD ROBERT A ,  BEN-YOSEF NISSIM

IPC: C12Q1/68 ,  G01J3/02 ,  G01J3/12 ,  G01J3/26 ,  G01J3/28 ,  G01J3/44 ,  G01J3/453 ,  G01J3/457 ,  G01N21/64 ,  G01N33/50 ,  G01N33/569 ,  G06K9/00 ,  G06K9/76 ,  G02B21/00

Abstract: A method and hardware for chromosome classification by decorrelation statistical analysis to provide color (spectral) karyotypes and to detect chromosomal aberrations.

公开(公告)号：IL102531A

公开(公告)日：1997-06-10

申请号：IL10253192

申请日：1992-07-17

Applicant: APPLIED SPECTRAL IMAGING LTD

IPC: G01B9/02 ,  G01N21/45 ,  G02B17/08

Abstract: A method according to Claim 2 of Patent Application 97328, wherein the OPD (Optical Path Difference) is varied by rotating a beam splitter about an axis perpendicular to the optical path.

公开(公告)号：IL192057D0

公开(公告)日：2008-12-29

申请号：IL19205708

申请日：2008-06-11

Applicant: APPLIED SPECTRAL IMAGING LTD ,  TSAFRIR KOLATT

IPC: C12Q20100101

公开(公告)号：IL142567A

公开(公告)日：2007-02-11

申请号：IL14256701

申请日：2001-04-12

Applicant: APPLIED SPECTRAL IMAGING LTD

IPC: C12Q1/68 ,  C12Q1/6841 ,  C12Q1/6883 ,  G01J3/02 ,  G01J3/12 ,  G01J3/26 ,  G01J3/28 ,  G01J3/44 ,  G01J3/453 ,  G01N1/30 ,  G01N21/64 ,  G01N21/78 ,  G01N33/50 ,  G01N33/53 ,  G01N33/533 ,  G01N33/535 ,  G01N33/569 ,  G01N33/58 ,  G06K9/00 ,  G06K9/76

Abstract: A method of in situ analysis of a biological sample comprising the steps of (a) staining the biological sample with N stains of which a first stain is selected from the group consisting of a first immunohistochemical stain, a first histological stain and a first DNA ploidy stain, and a second stain is selected from the group consisting of a second immunohistochemical stain, a second histological stain and a second DNA ploidy stain, with provisions that N is an integer greater than three and further that (i) if the first stain is the first immunohistochemical stain then the second stain is either the second histological stain or the second DNA ploidy stain; (ii) if the first stain is the first histological stain then the second stain is either the second immunohistochemical stain or the second DNA ploidy stain; whereas (iii) if the first stain is the first DNA ploidy stain then the second stain is either the second immunohistochemical stain or the second histological stain; and (b) using a spectral data collection device for collecting spectral data from the biological sample, the spectral data collection device and the N stains are selected such that a spectral component associated with each of the N stains is collectable.

公开(公告)号：DE69629374T2

公开(公告)日：2004-06-24

申请号：DE69629374

申请日：1996-12-10

Applicant: APPLIED SPECTRAL IMAGING LTD

Inventor： GARINI YUVAL ,  CABIB DARIO ,  BUCKWALD A ,  SOENKSEN G ,  KATZIR NIR ,  WINE DAVID ,  LAVI MOSHE

IPC: G01B9/02 ,  A61B3/12 ,  A61B5/00 ,  C12Q1/68 ,  G01B11/06 ,  G01J3/02 ,  G01J3/12 ,  G01J3/26 ,  G01J3/28 ,  G01J3/44 ,  G01J3/453 ,  G01N21/27 ,  G01N21/64 ,  G01N33/48 ,  G01N33/50 ,  G01N33/569 ,  G01N33/58 ,  G06K9/00 ,  G06K9/76

Abstract: An apparatus for use in a method of detecting and analysing fluorescent in-situ hybridisations (fig. 5) employing numerous chromosome paints (fig. 9) each labelled with a different fluorophore or combination of fluorophores, the apparatus being highly sensitive both in spatial and spectral resolutions (fig. 6) such that it is capable of simultaneous detection of dozens of fluorophores or combinations of fluorophores (fig. 7) so as to enable the detection of a complete set of fluorescently painted human chromosomes (fig. 10).

公开(公告)号：IL136190A

公开(公告)日：2004-02-08

申请号：IL13619098

申请日：1998-11-27

Applicant: APPLIED SPECTRAL IMAGING LTD

IPC: G01J3/46 ,  C12Q1/68 ,  G01B11/06 ,  G01J3/02 ,  G01J3/12 ,  G01J3/26 ,  G01J3/28 ,  G01J3/44 ,  G01J3/453 ,  G01J5/10 ,  G01N1/30 ,  G01N21/27 ,  G01N21/64 ,  G01N21/78 ,  G01N33/483 ,  G01N33/50 ,  G01N33/569 ,  G01N33/58 ,  G06K9/00 ,  G06K9/76 ,  G06T1/00

Abstract: A method for cancer cell detection including the steps of (a) staining an analyzed sample with at least first and second dyes, the dyes being selected such that the first dye better adheres to normal cells whereas the second dye better adheres to cancer cells; (b) spectrally imaging the sample through an optical device being optically connected to an imaging spectrometer thereby obtaining a spectrum of each pixel of the sample; (c) based on the spectra, evaluating concentrations of the first and second dyes for each of the pixels; and (d) based on the concentrations detecting the presence of cancer cells in the sample.

公开(公告)号：ES2188065T3

公开(公告)日：2003-06-16

申请号：ES99111903

申请日：1993-07-22

Applicant: APPLIED SPECTRAL IMAGING LTD

Inventor： CABIB DARIO ,  FRIEDMAN ZVI ,  LIPSON STEPHEN G ,  BUCKWALD ROBERT A

IPC: G01J3/26 ,  G01J3/28

公开(公告)号：ES2186097T3

公开(公告)日：2003-05-01

申请号：ES98306743

申请日：1998-08-24

Applicant: APPLIED SPECTRAL IMAGING LTD

Inventor： KATZIR NIR ,  GARINI YUVAL ,  WINE DAVID ,  CABIB DARIO

IPC: G01N15/14 ,  G01N21/78 ,  G01N21/64 ,  G01N33/533 ,  G01N33/566 ,  G01N33/58 ,  G02B21/06 ,  G01J3/28

Abstract: Classification of pixels into groups of pixels according to their association with a single fluorophore or a combination of fluorophores, comprises: (a) providing a number of wide-band emission filters; (b) for each of the pixels, recording emitted light intensity as retrieved after passing through each one of the emission filters, such that each of the pixels is representable by a vector of a number of dimensions equal to the number of wide-band emission filters; and (c) using an algorithm for evaluating the presence of each of the fluorophores associated with each of the pixels, thus classifying each of the pixels into a group of pixels according to its association with the fluorophores. Each fluorophore has a characterising emission spectrum and a specifying emission peak.

公开(公告)号：DE69719966D1

公开(公告)日：2003-04-24

申请号：DE69719966

申请日：1997-04-16

Applicant: APPLIED SPECTRAL IMAGING LTD

Inventor： GARINI YUVAL ,  CABIB DARIO ,  BUCKWALD ROBERT A ,  SOENKSEN DIRK G ,  RIED THOMAS

IPC: C12N15/09 ,  C12Q1/68 ,  G01J3/02 ,  G01J3/12 ,  G01J3/26 ,  G01J3/28 ,  G01J3/44 ,  G01J3/453 ,  G01N21/64 ,  G01N33/50 ,  G01N33/569 ,  G01N33/58 ,  G02B21/16 ,  G06K9/00 ,  G06K9/76 ,  G02B21/00

Abstract: A spectral imaging method for simultaneous detection of multiple fluorophores aimed at detecting and analyzing fluorescent in situ hybridizations employing numerous chromosome paints and/or loci specific probes each labeled with a different fluorophore or a combination of fluorophores for color karyotyping, and at multicolor chromosome banding, wherein each chromosome acquires a specifying banding pattern, which pattern is established using groups of chromosome fragments labeled with various fluorophore or combinations of fluorophores.

公开(公告)号：IL131953A

公开(公告)日：2002-08-14

申请号：IL13195398

申请日：1998-03-16

Applicant: APPLIED SPECTRAL IMAGING LTD

IPC: G01B9/02 ,  C12N5/06 ,  C12Q1/68 ,  G01J3/02 ,  G01J3/12 ,  G01J3/26 ,  G01J3/28 ,  G01J3/44 ,  G01J3/453 ,  G01J5/10 ,  G01N15/14 ,  G01N21/27 ,  G01N21/64 ,  G01N33/48 ,  G01N33/50 ,  G01N33/569 ,  G01N33/58 ,  G06K9/00 ,  G06K9/76

Abstract: According to the present invention there is provided a spectral bio-imaging methods which can be used for automatic and/or semiautomatic spectrally resolved morphometric classification of cells, the method comprising the steps of (a) preparing a sample to be spectrally imaged, the sample including at least a portion of at least one cell; (b) viewing the sample through an optical device, the optical device being optically connected to an imaging spectrometer, the optical device and the imaging spectrometer being for obtaining a spectrum of each pixel of the sample; (c) classifying each of the pixels into classification groups according to the pixels spectra; and (d) analyzing the classification groups and thereby classifying the at least one cell into a cell class.