公开(公告)号：WO1983002008A1

公开(公告)日：1983-06-09

申请号：PCT/US1982001714

申请日：1982-12-03

Applicant: BECKMAN INSTRUMENTS, INC.

Inventor： BECKMAN INSTRUMENTS, INC. ,  STEPHENS, Donald, E. ,  SHARPLES, Thomas, D.

IPC: G01N31/08

CPC classification number: G01N30/30 ,  G01N30/6026 ,  G01N30/6052 ,  G01N2030/303 ,  G01N2030/3084

Abstract: Colonne droite allongée (42) destinée à être utilisée en tant que réacteur dans un instrument analytique. La colonne (42) contient un matériau de garniture inerte permettant d'obtenir la longueur de chemin d'écoulement nécessaire pour un courant de fluide pénétrant dans le réacteur. Des ajustages (58) sont incorporés dans les extrémités de la colonne (42) de sorte que leur largeur ne dépasse pas la largeur de la colonne (42). Cette configuration de colonne (42) pour un réacteur autorise une plus grande souplesse dans le choix des mécanismes régulateurs de température utilisés pour chauffer et refroidir le réacteur.

公开(公告)号：WO1998021595A1

公开(公告)日：1998-05-22

申请号：PCT/US1997020640

申请日：1997-11-13

Applicant: BECKMAN INSTRUMENTS, INC.

Inventor： BECKMAN INSTRUMENTS, INC. ,  FARREN, Carl, A.

IPC: G01N35/10

CPC classification number: G01N35/1079 ,  G01N35/10

Abstract: The invention provides an apparatus for piercing the cap of a container. The apparatus includes a blade support arm slidably attached to one or more vertical posts, a blade attached vertically below the blade support arm, and a cap retainer arm slidably attached to the vertical post such that the cap retainer arm is capable of travel along the posts between an upper position wherein the cap contacting surface is located above the elevation of the container cap and a lower position wherein the cap contacting surface is located at the elevation of the container cap. The cap retainer arm has an opening through which the blade can pass. A biasing member biases the cap retainer arm towards its lower position. The cap retainer arm is positioned below the blade but above the blade support arm contact surface. Thus, when the blade support arm slides upwardly, the blade support arm contacts the cap retainer arm and pushes the cap retainer arm upwardly from its lower position to its upper position.

Abstract translation: 本发明提供了一种用于刺穿容器盖的装置。 该装置包括可滑动地附接到一个或多个垂直柱的叶片支撑臂，垂直于叶片支撑臂下方附接的叶片，以及可滑动地附接到竖直柱的盖保持臂，使得盖保持臂能够沿着柱 在盖接触表面位于容器盖的高度上方的上部位置和帽接触表面位于容器盖的高度处的下部位置之间。 盖保持臂具有刀片可以穿过的开口。 偏置构件将盖保持臂朝向其下部位置偏压。 盖保持臂位于刀片下方，但位于刀片支撑臂接触表面之上。 因此，当叶片支撑臂向上滑动时，叶片支撑臂接触盖保持臂，并将盖保持臂从其下部位置向上推动到其上部位置。

公开(公告)号：WO1997046874A1

公开(公告)日：1997-12-11

申请号：PCT/US1997009153

申请日：1997-05-30

Applicant: BECKMAN INSTRUMENTS, INC.

Inventor： BECKMAN INSTRUMENTS, INC. ,  XIE, Youquin, E. ,  YIN, Dean, Y. ,  NGUYEN, Khan, V. ,  SHU, Frank, R.

IPC: G01N27/48

CPC classification number: G01N27/3271

Abstract: A polarographic sensor device to determine the partial pressure of a gas in a sample medium is disclosed. The device comprises a) a substantially hydroscopic electrolyte composition joining a pair of spaced apart electrodes, one of the pair of electrodes being a sensing electrode; b) a membrane permeable to gas but impermeable to the electrolyte, the membrane having a front section for separating the electodes and the electrolyte from the sample medium; and c) a fastener comprising a sleeve having a body portion and a lip, the body portion retaining the fastener and a side section of the membrane, and the lip retaining a portion of the front section of the membrane and holding substantially the entire front section in a spaced apart relationship from the sensing electrode.

Abstract translation: 公开了一种确定样品介质中的气体的分压的极谱传感器装置。 所述装置包括a）基本上吸湿的电解质组合物，其接合一对间隔开的电极，所述一对电极中的一个是感测电极; b）可渗透气体但对电解质不透气的膜，该膜具有用于将电极和电解质与样品介质分离的前部分; 以及c）紧固件，其包括具有主体部分和唇缘的套筒，所述主体部分保持所述紧固件和所述膜的侧部，并且所述唇部保持所述膜的前部的一部分并且基本上保持所述整个前部 与感测电极间隔开的关系。

公开(公告)号：WO1997046597A1

公开(公告)日：1997-12-11

申请号：PCT/US1997008880

申请日：1997-05-22

Applicant: BECKMAN INSTRUMENTS, INC.

Inventor： BECKMAN INSTRUMENTS, INC. ,  MILTON, Raymond, C.

IPC: C08F08/10

CPC classification number: G01N33/545 ,  B01J2219/00722 ,  B01J2219/00725 ,  C07B2200/11 ,  C07H21/00 ,  C08F8/18 ,  C40B40/06 ,  C40B40/10 ,  G01N33/54353 ,  C08F10/00

Abstract: Reagents for the immobilization of biopolymers, processes for their preparation and their subsequent use in the immobilized of biopolymers for analytical and diagnostic procedures are described. One type of reagent includes a solid support fabricated of a polymeric material having at least one surface with pendant acyl fluoride functionalities. Another reagent includes solid supports fabricated of polymeric materials including ethylene acrylic acid or ethylene methacrylic acid copolymers and activated polypropylene. Processes for preparing reagents include derivatizing polymeric materials to form acyl fluoride functionalities or derivatizing ethylene acrylic acid copolymers and ethylene methacrylic acid copolymers to form active acyl functionalities. Processes for immobilizing biopolymers include attaching natural or presynthesized biopolymers to activated solid support surfaces and directly attaching in a step-wise successive manner biomonomer units to a growing biopolymer chain attached to the solid support reagent.

Abstract translation: 描述了用于固定生物聚合物的试剂，其制备方法及其随后用于固定化生物聚合物的分析和诊断程序。 一种类型的试剂包括由具有至少一个具有侧酰酰氟官能度的表面的聚合物材料制成的固体支持体。 另一种试剂包括由包括乙烯丙烯酸或乙烯甲基丙烯酸共聚物和活化聚丙烯的聚合材料制成的固体载体。 用于制备试剂的方法包括衍生化聚合物材料以形成酰基氟官能团或衍生化乙烯丙烯酸共聚物和乙烯甲基丙烯酸共聚物以形成活性酰基官能团。 用于固定生物聚合物的方法包括将天然或预合成的生物聚合物连接到活化的固体支持物表面，并且以逐步连续的方式将生物单体单元直接连接到附着于固体支持剂的生长生物聚合物链上。

公开(公告)号：WO1997026542A1

公开(公告)日：1997-07-24

申请号：PCT/US1997000443

申请日：1997-01-14

Applicant: BECKMAN INSTRUMENTS, INC.

Inventor： BECKMAN INSTRUMENTS, INC. ,  MC NEAL, Jack, D.

IPC: G01N35/10

CPC classification number: G01N35/1016 ,  G01L7/02 ,  G01L9/0001 ,  G01N2035/1018

Abstract: A device (10) is provided for detecting a flow restriction in pipetting transporting a fluid sample (14) for a diagnostic system. The device (10) comprises a hollow conduit (12) which transports the fluid sample (14), a housing (16), a substantially incompressible medium (18), and a sensor (20). The hollow conduit (12) includes a flexible section (24), i.e., a piece of thin-walled tubing made from an elastomer. The conduit (12) has a substantially constant internal cross-sectional area. The housing (16) has an internal cavity (60) which substantially encloses the flexible section (24) and forms a sealed internal chamber (62) around the flexible section (24). The substantially incompressible medium (18) fills the internal chamber (62). The sensor (20) is in communication with the substantially incompressible medium (18) and detects a change of pressure of the substantially incompressible medium (18). A flow restriction in the conduit (12) causes an increase of pressure in the conduit (12) which causes the flexible section (24) to expand. Expansion of the flexible section (24) results in an increase of pressure in the substantially incompressible medium (18) in the internal chamber (62) which is detected by the sensor (20).

Abstract translation: 提供了一种装置（10），用于检测运送用于诊断系统的流体样品（14）的移液中的流量限制。 装置（10）包括输送流体样品（14），壳体（16），基本不可压缩的介质（18）和传感器（20）的中空导管（12）。 中空管道（12）包括柔性部分（24），即由弹性体制成的一片薄壁管。 导管（12）具有基本恒定的内部横截面积。 壳体（16）具有内部空腔（60），其基本上包围柔性部分（24）并围绕柔性部分（24）形成密封的内部腔室（62）。 基本上不可压缩的介质（18）填充内部室（62）。 传感器（20）与基本上不可压缩的介质（18）连通，并检测基本不可压缩介质（18）的压力变化。 导管（12）中的流动限制导致导管（12）中的压力增加，导致柔性部分（24）膨胀。 柔性部分（24）的膨胀导致由传感器（20）检测到的内部腔室（62）中的基本不可压缩的介质（18）中的压力增加。

公开(公告)号：WO1997022006A1

公开(公告)日：1997-06-19

申请号：PCT/US1996016895

申请日：1996-10-22

Applicant: BECKMAN INSTRUMENTS, INC.

Inventor： BECKMAN INSTRUMENTS, INC. ,  BELL, Michael, L.

IPC: G01N35/02

CPC classification number: G01N35/025 ,  Y10T436/11 ,  Y10T436/113332 ,  Y10T436/114998 ,  Y10T436/115831 ,  Y10T436/2575

Abstract: An improved automated diagnostic analyzer and method of the type which includes a reaction carousel having cuvettes to receive sample and reactant for photometric analysis and to index and park for addition of sample, reactant and washing of cuvettes. Alternative reactant and/or sample addition points are provided so the analyzer controller may index cuvettes to provide greater frequency at which washing of cuvettes may take place simultaneously with addition of sample or reactant.

Abstract translation: 一种改进的自动诊断分析仪和方法，其包括反应转盘，其具有用于接收样品的反应杯和用于光度分析的反应物，并用于指示和停放添加样品，反应物和洗涤比色杯。 提供替代的反应物和/或样品添加点，因此分析仪控制器可以对比色皿进行分析以提供更大的频率，在添加样品或反应物的同时可以同时进行洗涤。

公开(公告)号：WO1997012679A1

公开(公告)日：1997-04-10

申请号：PCT/US1996015330

申请日：1996-09-25

Applicant: BECKMAN INSTRUMENTS, INC.

Inventor： BECKMAN INSTRUMENTS, INC. ,  MC NEAL, Jack, D. ,  BELL, Michael, L.

IPC: B01L03/14

CPC classification number: B01L3/5021 ,  G01N33/491 ,  Y10T436/25375

Abstract: A method for rapid separation of serum or plasma from cells in a whole blood sample uses a cylindrical sample tube having longitudinally-extending internal ribs. A separation gel and the sample are introduced into the tube, and the tube is axially centrifuged so as to form concentric shells of cells, gel, and serum or plasma. After centrifugation is stopped, the serum or plasma collects in the tube by gravity flow and the cells and gel remain affixed to the walls and ribs of the tube.

Abstract translation: 在全血样品中快速分离血清或血浆中的细胞的方法使用具有纵向延伸的内部肋的圆柱形样品管。 将分离凝胶和样品引入管中，并将管轴向离心以形成细胞，凝胶和血清或血浆的同心圆壳。 离心停止后，血清或血浆通过重力流收集在管中，细胞和凝胶保持固定在管的壁和肋上。

公开(公告)号：WO1996023221A1

公开(公告)日：1996-08-01

申请号：PCT/US1996001347

申请日：1996-01-23

Applicant: BECKMAN INSTRUMENTS, INC. ,  NORTHEASTERN UNIVERSITY ,  NORTHEASTERN UNIVERSITY ,  KARGER, Barry, L. ,  GOETZINGER, Wolfgang ,  MUTH, Jochen ,  SHIEH, Chia-Hui

Inventor： BECKMAN INSTRUMENTS, INC. ,  NORTHEASTERN UNIVERSITY ,  NORTHEASTERN UNIVERSITY

IPC: G01N27/477

CPC classification number: G01N27/44704 ,  G01N27/44752

Abstract: Coated capillary electrophoresis columns and methods for their use in electrophoretic separations are disclosed. The coated capillary columns include a length of tubing having an interior surface having an interconnected polymeric coating. The interconnected polymeric coating includes a hydrophobic hydrocarbon polymeric functionality covalently bound to the interior surface and polyvinylalcohol interconnected with the hydrophobic polymeric functionality. Exemplary columns are prepared by causing a Si-OH reactive compound, having a hydrophobic polymeric functionality, to react with Si-OH functionalities on the interior surface of capillary columns. Then causing vinyl acetate to polymerize in contact with the hydrophobic polymeric functionality and hydrolyzing the resulting polyvinylacetate forms a coating of interconnecting hydrophilic and hydrophobic polymers. Alternative exemplary coatings include a modified polyvinylalcohol such as a polyvinylether.

Abstract translation: 公开了涂布毛细管电泳柱及其用于电泳分离的方法。 涂覆的毛细管柱包括具有内表面的一段长度的管，其具有互连的聚合物涂层。 相互连接的聚合物涂层包括与内表面共价结合的疏水性烃聚合物官能团和与疏水性聚合物官能团互连的聚乙烯醇。 通过使具有疏水聚合官能度的Si-OH反应性化合物与毛细管柱内表面上的Si-OH官能团反应来制备示例性柱。 然后使乙酸乙烯酯与疏水聚合官能团接触聚合并水解所得聚乙酸乙烯酯形成互连的亲水和疏水聚合物的涂层。 替代的示例性涂层包括改性的聚乙烯醇，例如聚乙烯醚。

公开(公告)号：WO1996022524A1

公开(公告)日：1996-07-25

申请号：PCT/US1995016940

申请日：1995-12-20

Applicant: BECKMAN INSTRUMENTS, INC.

Inventor： BECKMAN INSTRUMENTS, INC. ,  KEO, Nida ,  SAFARIAN, Zara ,  LIU, Cheng-Ming ,  WANG, HANN-Ping

IPC: G01N27/447

CPC classification number: G01N27/44747

Abstract: A buffer and method useful for the analysis of glycoproteins by capillary zone electrophoresis. The buffer comprises water, a sugar complexing compound, a base compound for adjusting the pH, and a zwitterionic compound. An embodiment of the buffer comprises sodium borate as the complexing compound, sodium hydroxide as the base, and 3-cyclohexylamino-1-propanesulfonic acid as the zwitterionic compound. In the method, a selected glycoprotein is subjected to capillary zone electrophoresis. The proportion or the amount of the glycoprotein is determined by quantitative analysis of the resulting electropherogram.

Abstract translation: 用于通过毛细管区带电泳分析糖蛋白的缓冲液和方法。 缓冲液包括水，糖络合化合物，用于调节pH的碱性化合物和两性离子化合物。 缓冲剂的一个实施方案包括作为络合化合物的硼酸钠，作为碱的氢氧化钠和作为两性离子化合物的3-环己基氨基-1-丙磺酸。 在该方法中，将选择的糖蛋白进行毛细管区带电泳。 通过对所得电泳图的定量分析来确定糖蛋白的比例或量。

公开(公告)号：WO1996006174A1

公开(公告)日：1996-02-29

申请号：PCT/US1994009450

申请日：1994-08-18

Applicant: BECKMAN INSTRUMENTS, INC.

Inventor： BECKMAN INSTRUMENTS, INC. ,  ADAMS, Craig, W. ,  PANG, Patty, P., Y. ,  BELEI, Marina, C.

IPC: C12N15/55

CPC classification number: C12N9/22 ,  A61K39/00 ,  C07K2319/00 ,  C07K2319/02

Abstract: The gene for Streptococcus pyogenes DNase B has been cloned and vectors incorporating the cloned DNA have been used to transform Escherichia coli, allowing efficient and rapid production of the DNase in E. coli without the necessity of growing large quantities of S. pyogenes. The enzyme can be produced with aleader peptide at its amino terminus. An improved method for the purification of naturally occurring S. pyogenes DNase B enzyme is also provided. The DNase B enzyme produced, either by purification of naturally occurring enzyme or by recombinant DNA techniques, can be used to generate antibodies and can also be used in immunochemical assays to detect the presence of anti-DNase B antibodies in serum as a marker of infection by S. pyogenes.

Abstract translation: 已经克隆了化脓性链球菌DNase B的基因，并且已经使用掺入克隆DNA的载体来转化大肠杆菌，从而能够在大肠杆菌中高效快速地生产DNA酶，而不需要生长大量的化脓性链球菌。 该酶可以在其氨基末端用引入肽产生。 还提供了用于纯化天然存在的化脓性链球菌DNA酶B酶的改进方法。 通过纯化天然存在的酶或通过重组DNA技术产生的DNA酶B酶可用于产生抗体，也可用于免疫化学测定以检测血清中抗DNase B抗体作为感染标志物的存在 化脓性链球菌。