公开(公告)号：WO1996022524A1

公开(公告)日：1996-07-25

申请号：PCT/US1995016940

申请日：1995-12-20

Applicant: BECKMAN INSTRUMENTS, INC.

Inventor： BECKMAN INSTRUMENTS, INC. ,  KEO, Nida ,  SAFARIAN, Zara ,  LIU, Cheng-Ming ,  WANG, HANN-Ping

IPC: G01N27/447

CPC classification number: G01N27/44747

Abstract: A buffer and method useful for the analysis of glycoproteins by capillary zone electrophoresis. The buffer comprises water, a sugar complexing compound, a base compound for adjusting the pH, and a zwitterionic compound. An embodiment of the buffer comprises sodium borate as the complexing compound, sodium hydroxide as the base, and 3-cyclohexylamino-1-propanesulfonic acid as the zwitterionic compound. In the method, a selected glycoprotein is subjected to capillary zone electrophoresis. The proportion or the amount of the glycoprotein is determined by quantitative analysis of the resulting electropherogram.

Abstract translation: 用于通过毛细管区带电泳分析糖蛋白的缓冲液和方法。 缓冲液包括水，糖络合化合物，用于调节pH的碱性化合物和两性离子化合物。 缓冲剂的一个实施方案包括作为络合化合物的硼酸钠，作为碱的氢氧化钠和作为两性离子化合物的3-环己基氨基-1-丙磺酸。 在该方法中，将选择的糖蛋白进行毛细管区带电泳。 通过对所得电泳图的定量分析来确定糖蛋白的比例或量。

公开(公告)号：EP0750745A1

公开(公告)日：1997-01-02

申请号：EP95944411.0

申请日：1995-12-20

Applicant: BECKMAN INSTRUMENTS, INC.

Inventor： KEO, Nida ,  SAFARIAN, Zara ,  LIU, Cheng-Ming ,  WANG, HANN-Ping

IPC: G01N33 ,  G01N27

CPC classification number: G01N27/44747

Abstract: A buffer and method useful for the analysis of glycoproteins by capillary zone electrophoresis. The buffer comprises water, a sugar complexing compound, a base compound for adjusting the pH, and a zwitterionic compound. An embodiment of the buffer comprises sodium borate as the complexing compound, sodium hydroxide as the base, and 3-cyclohexylamino-1-propanesulfonic acid as the zwitterionic compound. In the method, a selected glycoprotein is subjected to capillary zone electrophoresis. The proportion or the amount of the glycoprotein is determined by quantitative analysis of the resulting electropherogram.

Abstract translation: 用于通过毛细管区带电泳分析糖蛋白的缓冲液和方法。 该缓冲液包含水，糖络合化合物，用于调节pH的碱化合物和两性离子化合物。 缓冲剂的一个实施方案包含作为络合化合物的硼酸钠，作为碱的氢氧化钠和作为两性离子化合物的3-环己基氨基-1-丙烷磺酸。 在该方法中，将选择的糖蛋白进行毛细管区带电泳。 通过定量分析所得电泳图确定糖蛋白的比例或量。