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    System and methods for dynamic range extension using variable lenght integration time sampling
    71.
    发明公开
    System and methods for dynamic range extension using variable lenght integration time sampling 审中-公开
    系统和Verfahren zur dynamischen Bereichserweiterungüber整合采样von variablerLänge

    公开(公告)号：EP1903331A1

    公开(公告)日：2008-03-26

    申请号：EP07024650.9

    申请日：2003-10-08

    Applicant: Applera Corporation

    Inventor： Sagatelyan, Dmitry M. ,  Slettnes, Tor

    IPC: G01N21/64 ,  G01J1/42 ,  H04N5/235 ,  H04N3/15

    CPC classification number: G01N21/274 ,  G01J1/42 ,  G01N21/645 ,  G01N2201/1241 ,  H03F3/08 ,  H04N5/235 ,  H04N5/2353 ,  H04N5/2355 ,  H04N5/243 ,  H04N5/35581

    Abstract: A photo-detector (122) generated signal (125) is measured as a sample set (192) comprising a long signal and a short signal. The short signal is scaled to the value of the long signal if the long signal exceeds a dynamic range (131) associated with the photo-detector (122). In one embodiment, the short signal is obtained during a short time interval that is at the approximate middle of a long time interval such that the short and long intervals share a common median time value (194). Given such symmetry, an approximately linear signal (190) yields a proportionality parameter between the long and short signals thereby allowing the short signal to be scaled. The proportionality parameter facilitates determination of an integration independent component of the photo-detector signal that should be removed from the measured long and short signals before scaling. A plurality of sample sets (260) can also be processed such that each sample set overlaps with its neighboring sample set, thereby increasing the effective number of sample sets.

    Abstract translation: 测量光检测器（122）产生的信号（125）作为包括长信号和短信号的采样组（192）。 如果长信号超过与光检测器（122）相关联的动态范围（131），则短信号被缩放到长信号的值。 在一个实施例中，在短时间间隔内获得短信号，该短时间间隔在长时间间隔的大致中间处，使得短间隔和长间隔共享公共中值时间值（194）。 给定这样的对称性，近似线性信号（190）产生长信号和短信号之间的比例参数，从而允许缩短短信号。 比例参数有助于确定光电探测器信号的积分独立分量，该分量应在缩放之前从被测量的长信号和短信号中去除。 还可以处理多个样本集（260），使得每个样本集合与其相邻样本集重叠，从而增加样本集合的有效数量。

    Method to avoid signals-saturation in the quantification of polynucleotide microarrays, a device, a computer program and data therefor
    73.
    发明公开
    Method to avoid signals-saturation in the quantification of polynucleotide microarrays, a device, a computer program and data therefor 审中-公开
    方法，装置，和用于避免微阵列的多核苷酸定量信号饱和度的计算机程序

    公开(公告)号：EP1394716A1

    公开(公告)日：2004-03-03

    申请号：EP02447151.8

    申请日：2002-08-07

    Applicant: Université Libre de Bruxelles-Hôpital Erasme

    Inventor： Venet, David ,  Maenhaut, Carine Estelle ,  Bersini, Hugues

    IPC: G06F19/00

    CPC classification number: G01N21/64 ,  G01N2201/1241 ,  G06F19/20 ,  G06F19/24

    Abstract: The invention relates to a method for avoiding signal-saturation in the quantification of polynucleotide microarrays, a device, a computer program and data therefor. The invention quantitatively measures an optical property of a set of samples, said optical property relating to the concentration of the macromolecule to be determined, using an optical detector at two or more different detector gains. The invention uses an algorithm comprising 1) an equation which relates the real intensity to detector gain and measured intensity and 2) minimisation of said equation, and 3) two data matrices which are iteratively modified so as to exclude samples which are saturated, as judged by their predicted intensity. Each iteration of the algorithm improves the prediction and the matrices are concomitantly modified so as to re-include or exclude samples which are saturated. The invention discloses initialisation and normalisation procedures.

    Abstract translation: 本发明涉及一种用于在多核苷酸微阵列，装置，计算机程序和数据为此的量化避免信号饱和的方法。 对本发明的一组样品的光学特性定量地测量，所述光学性质与大分子的浓度是确定的开采，利用在光检测器在两个或多个不同的检测器的增益。 算法，其包含1）到等式其涉及真正的强度检测器的增益和测量的强度和2）所述等式的最小化，以及本发明使用3），其被迭代修改，以便排除哪些是饱和的样品，来判断两个数据矩阵 他们用强度预测。 该算法的每次迭代改进了预测和矩阵伴随修改以重新包括或排除哪些是饱和的样品。 本发明光盘松散初始化和归一化过程。

    A method of preselecting flashlamp voltages for assays
    74.
    发明公开
    A method of preselecting flashlamp voltages for assays 有权
    预先选择闪光灯电压进行检测的方法

    公开(公告)号：EP0979993A2

    公开(公告)日：2000-02-16

    申请号：EP99306364.3

    申请日：1999-08-12

    Applicant: Ortho-Clinical Diagnostics, Inc.

    Inventor： Chiapperi, Joseph M. ,  Josephson, Donald M. ,  MacDonald, Stuart G.

    IPC: G01N21/25 ,  G01J3/10

    CPC classification number: G01J3/10 ,  G01N21/274 ,  G01N2021/3174 ,  G01N2201/0694 ,  G01N2201/1241 ,  G01N2201/12776

    Abstract: A method of detecting an optical change in a series of test assays producing detectable results at varying efficiencies, the method comprising the steps of: a) selecting a test assay from the series, the selected assay having a known end-point photoresponse efficiency and a known filter center wavelength; b) providing a variable-intensity flash lamp illuminator comprising a lamp, a set of multiple filters with pre-selected center wavelengths assigned to particular assays, and a circuit for activating the lamp and comprising a capacitor, a power source, and a variable output voltage converter connected to the source and having its variable voltage output connected across the capacitor, the lamp and the filters providing a known level of system efficiency as a function of the center wavelength of the filter; c) providing a predetermined relationship of levels of illuminating intensities from the lamp as a function of photoresponse efficiencies of the assays and the system efficiencies, in which the photoresponse efficiencies of the assays are inversely proportional to the lamp intensities and the intensities are proportional to the square of the voltages applied to the lamp; d) selecting from the relationship a voltage applied to the lamp, and hence an intensity of the lamp, that corresponds to the known photoresponse efficiency of the assay selected in step (a) and its system efficiency based upon the filter center wavelength for the assay; and e) thereafter exposing the assay to the selected illuminating intensity, so that less intensity is used for assays having either higher photoresponse efficiencies or center wavelengths with a higher system efficiency, or both, than is used for worst-case efficiency assays.

    Abstract translation: 一种检测一系列测试测定中的光学变化的方法，该方法以不同的效率产生可检测结果，该方法包括以下步骤：a）从系列中选择测试测定，所选测定具有已知的终点光响应效率和 已知滤波器中心波长; b）提供可变强度闪光灯照明器，其包括灯，具有分配给特定测定的预选中心波长的一组多个滤光器，以及用于激活灯并包括电容器，电源和可变输出的电路 电压转换器连接到电源并且其可变电压输出连接在电容器两端，灯和滤波器提供作为滤波器的中心波长的函数的已知水平的系统效率; c）提供作为测定的光响应效率和系统效率的函数的来自灯的照明强度的预定关系，其中测定的光响应效率与灯强度成反比，并且强度与 施加于灯的电压的平方; d）从所述关系中选择施加到所述灯的电压，并且因此所述灯的强度，其对应于步骤（a）中选择的所述测定的已知光响应效率和基于所述测定的滤波器中心波长的其系统效率 ; 和e）此后使测定暴露于选择的照射强度，使得较少的强度用于具有比用于最坏情况效率测定的更高光响应效率或中心波长更高系统效率或两者的测定。

    Fluorimeter with intensity-staged excitation light and detection method
    75.
    发明公开
    Fluorimeter with intensity-staged excitation light and detection method 失效
    荧光计强度测定

    公开(公告)号：EP0886136A1

    公开(公告)日：1998-12-23

    申请号：EP98304689.7

    申请日：1998-06-15

    Applicant: Ortho-Clinical Diagnostics, Inc.

    Inventor： MacDonald, Stuart Gilmour

    IPC: G01N21/64 ,  G01J3/10

    CPC classification number: G01N21/64 ,  G01J3/10 ,  G01N21/645 ,  G01N2201/0696 ,  G01N2201/1241

    Abstract: A method and fluorimeter for flashing a target at several different levels for detection of fluorescence by a PMT without blinding the PMT at the highest level. Two lamps are provided each of which is powered to flash at two different levels that are staged in energy from the lowest of four to the highest of four levels, and a shutter is provided to close off the PMT from exposure when an acceptable, detectable level of fluorescence is detected by the PMT.

    Abstract translation: 一种用于在几个不同水平闪烁目标的方法和荧光计，用于通过PMT检测荧光，而不会使最高水平的PMT发生眩晕。 提供两个灯，每个灯被供电以两个不同的水平闪光，这两个不同的电平从4个电平的最低点到最高的4个电平进行，并且当可接受的可检测电平提供用于关闭PMT以防曝光的快门 的荧光被PMT检测到。

    SYSTEM AND METHODS FOR DYNAMIC RANGE EXTENSION USING VARIABLE LENGTH INTEGRATION TIME SAMPLING
    77.
    发明授权
    SYSTEM AND METHODS FOR DYNAMIC RANGE EXTENSION USING VARIABLE LENGTH INTEGRATION TIME SAMPLING 有权
    系统和方法用于扩展动态卷通过采样与积分时间可变长度USE

    公开(公告)号：EP1552279B1

    公开(公告)日：2008-07-23

    申请号：EP03770710.6

    申请日：2003-10-08

    Applicant: Applera Corporation

    Inventor： SAGATELYAN, Dmitry, M. ,  SLETTNES, Tor

    IPC: G01N21/64 ,  G01J1/42 ,  H04N3/15

    CPC classification number: G01N21/274 ,  G01J1/42 ,  G01N21/645 ,  G01N2201/1241 ,  H03F3/08 ,  H04N5/235 ,  H04N5/2353 ,  H04N5/2355 ,  H04N5/243 ,  H04N5/35581

    Abstract: A photo-detector 122 generated signal 125 is measured as a sample set 192 comprising a long signal and a short signal. The short signal is scaled to the value of the long signal if the long signal exceeds a dynamic range 131 associated with the photo detector 122. In one embodiment, the short signal is obtained during a short time interval that is at the approximate middle of a long time interval such that the short and long intervals share a common median time value 194. Given such symmetry, an approximately linear signal 190 yields a proportionality parameter between the long and short signals thereby allowing the short signal to be scaled. The proportionality parameter facilitates determination of an integration independent component of the photo detector signal that should be removed from the measured long and short signals before scaling. A plurality of sample sets 260 can also be processed such that each sample set overlaps with its neighboring sample set, thereby increasing the effective number of sample sets.

    Instrument and methods for analysing a multiplicity of fluorescent dyes using a multiplicity of photodetectors having adjustable gains
    78.
    发明公开
    Instrument and methods for analysing a multiplicity of fluorescent dyes using a multiplicity of photodetectors having adjustable gains 有权
    利用光电检测器的多个设备和方法，用于荧光染料的多重分析可变增益

    公开(公告)号：EP1431745A1

    公开(公告)日：2004-06-23

    申请号：EP03027367.6

    申请日：2003-11-27

    Applicant: Becton Dickinson and Company

    Inventor： Bishop, James, E. ,  Dalal, Sunil S. ,  Lock, Michael D. ,  Gong, Zhenxiang

    IPC: G01N15/14 ,  G01N21/64

    CPC classification number: G01N21/6428 ,  G01N15/1459 ,  G01N2021/6441 ,  G01N2201/1241

    Abstract: An instrument for analysing a multiplicity of fluorescent dyes is disclosed, said instrument comprising a multiplicity of photodetectors, each having an adjustable gain, and a stored representation of pairwise functional relationships between measured fluorescence and photodetector gain for each of said photodetectors and for each of said fluorescent dyes. Preferably, the instrument is a flow cytometer. Furthermore, methods are disclosed for calculating compensation/quantitation for arbitrary sets of photodetector gain settings which do not require the analysis of additional setup samples.

    Abstract translation: 仪成就分析荧光染料的多重性圆盘游离缺失，所述器械包括光电检测器的多个，每个在可调增益具有，和测得的荧光和光检测器的增益为每个所述光电检测器之间，并且对每个所述的成对的功能关系的一个存储的表示 荧光染料。 优选地，该仪器是流式细胞仪。 进一步，方法是游离缺失盘计算补偿/定量任意套不需要额外的样本设置的分析光检测器的增益设置。

    A method of preselecting flashlamp voltages for assays
    79.
    发明公开
    A method of preselecting flashlamp voltages for assays 有权
    一种用于选择某些测定的电压为闪光灯方法

    公开(公告)号：EP0979993A3

    公开(公告)日：2000-03-29

    申请号：EP99306364.3

    申请日：1999-08-12

    Applicant: Ortho-Clinical Diagnostics, Inc.

    Inventor： Chiapperi, Joseph M. ,  Josephson, Donald M. ,  MacDonald, Stuart G.

    IPC: G01N21/25 ,  G01J3/10

    CPC classification number: G01J3/10 ,  G01N21/274 ,  G01N2021/3174 ,  G01N2201/0694 ,  G01N2201/1241 ,  G01N2201/12776

    Abstract: A method of detecting an optical change in a series of test assays producing detectable results at varying efficiencies, the method comprising the steps of: a) selecting a test assay from the series, the selected assay having a known end-point photoresponse efficiency and a known filter center wavelength; b) providing a variable-intensity flash lamp illuminator comprising a lamp, a set of multiple filters with pre-selected center wavelengths assigned to particular assays, and a circuit for activating the lamp and comprising a capacitor, a power source, and a variable output voltage converter connected to the source and having its variable voltage output connected across the capacitor, the lamp and the filters providing a known level of system efficiency as a function of the center wavelength of the filter; c) providing a predetermined relationship of levels of illuminating intensities from the lamp as a function of photoresponse efficiencies of the assays and the system efficiencies, in which the photoresponse efficiencies of the assays are inversely proportional to the lamp intensities and the intensities are proportional to the square of the voltages applied to the lamp; d) selecting from the relationship a voltage applied to the lamp, and hence an intensity of the lamp, that corresponds to the known photoresponse efficiency of the assay selected in step (a) and its system efficiency based upon the filter center wavelength for the assay; and e) thereafter exposing the assay to the selected illuminating intensity, so that less intensity is used for assays having either higher photoresponse efficiencies or center wavelengths with a higher system efficiency, or both, than is used for worst-case efficiency assays.

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