公开(公告)号：EP1431745B1

公开(公告)日：2006-04-19

申请号：EP03027367.6

申请日：2003-11-27

Applicant: Becton Dickinson and Company

Inventor： Bishop, James, E. ,  Dalal, Sunil S. ,  Lock, Michael D. ,  Gong, Zhenxiang

IPC: G01N15/14 ,  G01N21/64

CPC classification number: G01N21/6428 ,  G01N15/1459 ,  G01N2021/6441 ,  G01N2201/1241

公开(公告)号：EP1431745A1

公开(公告)日：2004-06-23

申请号：EP03027367.6

申请日：2003-11-27

Applicant: Becton Dickinson and Company

Inventor： Bishop, James, E. ,  Dalal, Sunil S. ,  Lock, Michael D. ,  Gong, Zhenxiang

IPC: G01N15/14 ,  G01N21/64

CPC classification number: G01N21/6428 ,  G01N15/1459 ,  G01N2021/6441 ,  G01N2201/1241

Abstract: An instrument for analysing a multiplicity of fluorescent dyes is disclosed, said instrument comprising a multiplicity of photodetectors, each having an adjustable gain, and a stored representation of pairwise functional relationships between measured fluorescence and photodetector gain for each of said photodetectors and for each of said fluorescent dyes. Preferably, the instrument is a flow cytometer. Furthermore, methods are disclosed for calculating compensation/quantitation for arbitrary sets of photodetector gain settings which do not require the analysis of additional setup samples.

Abstract translation: 仪成就分析荧光染料的多重性圆盘游离缺失，所述器械包括光电检测器的多个，每个在可调增益具有，和测得的荧光和光检测器的增益为每个所述光电检测器之间，并且对每个所述的成对的功能关系的一个存储的表示 荧光染料。 优选地，该仪器是流式细胞仪。 进一步，方法是游离缺失盘计算补偿/定量任意套不需要额外的样本设置的分析光检测器的增益设置。