Abstract:
@ A blood processing sealless centrifuge has a sterile disposable tube assembly comprising a plastic lumen tube (3) encased adjacent its ends by spaced plastic reinforcing tubes (5, 8) having remote clamp ends (18, 19) and spaced thrust bearings (7, 10). The thrust bearings fit laterally into slots in a tube rotor (not shown) which rotates in the same direction as, but at half the speed of, the centrifuge rotor. The low-mass central portion of the lumen tube is unsupported except from the bearings and in operation it flies in a smooth bend. The lumen tube is easily placed in position with its bearings in the slots of the tube rotor, by side-entry, without dismantling the tube assembly and the bearings are subsequently held in place by beam strength and compression of the flexed reinforcing tubes and by centrifugal force.
Abstract:
Method and apparatus for generating centrifugal forces, including a plate rotatable about an axis, and at least one holding means mounted on the plate for rotation therewith, the holding means being adapted to receive an article to be subjected to centrifugal force and being rotatable relative to the plate member to permit the direction of centrifugal force acting on the article to be changed.
Abstract:
The invention provides a rotor head assembly (40) adapted for detachable mounting upon an upstanding rotatable electromagnet (18) of a decanting centrifuge (10), said rotor head assembly comprising an annular support member (46) and a plurality of magnetically-attractable open-topped tube carriers (43) pivotally suspended from said support member; each tube carrier including a tube-holding member (50) and a magnet-contacting member (51); said tube-holding member and magnet-contacting member each having an upper end portion independently and pivotally suspended from said annular support member and being adjustably connected to each other at their lower ends for selectively adjusting the maximum angle therebetween for controlling the discharge of fluid from a centrifuge tube supported by said tube-holding member during a decanting operation.
Abstract:
A rotor for a centrifuge is provided with a peripheral groove having confronting slots communicating therewith. A retaining member is disposed so as to overlie each of the slots by a predetermined radial distance. The radial dimension of each slot is greater than the sum of the radial distance by which the retaining member overlies the slot and the radial thickness dimension of a tube carrier receivable within the slots. Thus, the tube carrier is movable within the slots from a first, radially inner, position to a second radially outer, position in response to centrifugal force such that when in the second position movement of the tube carrier in a direction parallel to the axis of rotation is prohibited.
Abstract:
A cell washing apparatus having a pivotally movable sample tube holder provided with a radially inwardly directed arm having a grasping hook thereon. The hook engages a retaining surface disposed radially inwardly of the pivot point of the holder to restrain radial outward movement of the sample tube holder.
Abstract:
@ A fluid processing apparatus and method which utilizes centrifugation to separate constituent components of e.g. blood, in a flexible bag (8). The less dense separated components e.g., supernatant, is expressed from the bag (8) by centrifugal force acting on a plate (15) adjacent the bag and the more dense component (e.g. RBC's), remains. The bag (8) is oriented at a double angle in the centrifuge rotor (28) so that less dense component accumulates at one location (A) and more dense at a second location (C) diagonally opposite the first location, thus facilitating removal of the less dense component to a further bag (2) and washing of the more dense component in the bag (8) by wash solution expressed from a still further flexible bag (20) by centrifugal force acting on a plate (17) adjacent the bag.
Abstract:
Sedimentation field flow fractionation apparatus having a rotor-mounted fractionation column, has detectors positioned to observe the fluids from the column while still in the rotor. Designs include parallel reference and fractionation columns having their outflows compared in a rotor-mounted fluid flow density balance. Optionally weired channel columns provide a free fluid surface for more efficient resolution. Horizontal rotational axes are preferred. Rotor-mounted fluid flow density balances are also provided which are suitable for detecting liquid density changes in the other applications, such as liquid chromatography.
Abstract:
A system is disclosed for treating blood, with the objective of reducing the functioning population of a blood constituent. According to the method, blood is passed through an ultraviolet radiation field in the presence of a dissolved photoactive agent capable of forming photo- adducts with receptors on the constituent to effect covalent bonding between the photoactive agent and the same. If desired, the blood to be treated may be separated to provide a blood fraction rich in the constituent sought to be reacted and low in any blood component which absorbs UV radiation, as for example by continuous centrifuging, which fraction is then irradiated with UV radiation in the presence of the dissolved photoactive chemical agent.
Abstract:
A self-balancing contrifuge has a rotor (102) driven by a shaft (56) journalled in bearings (54, 114) supported by upper and lower flexible bearing mounts (120,48). The horizontally flexible bearing mounting planes thus provided give a greater degree of freedom for the axis of rotation (R) of the rotor to move into a coincident relationship with the angular momentum vector of the rotor as it changes with dynamic imbalance thereby to compensate for any imbalance which may occur in the centrifuge rotor during centrifuging. The centrifuge is particularly intended for use in processing blood.