Abstract:
Method and apparatus for generating centrifugal forces, including a plate rotatable about an axis, and at least one holding means mounted on the plate for rotation therewith, the holding means being adapted to receive an article to be subjected to centrifugal force and being rotatable relative to the plate member to permit the direction of centrifugal force acting on the article to be changed.
Abstract:
A shell-type centrifuge rotor (10) has a sample container support sleeve (30) extending through the cavity (24C) in a plate (24). The sleeve (30) has at least two slots (30C, 30D) which define at least one resilient flange (30F) pivotally deflectable about a pivot axis (30P) to hold the sleeve (30) in a fixed relationship with respect to the plate (24). In one embodiment, both slots (30C, 30D) extend axially along the sleeve (30). In an alternate embodiment, a first one of the slots (31) extends axially along the sleeve (30') and the second one of the slots (32) extends circumferentially along the sleeve (30'). In a modification to the alternate embodiment, a second circumferential slot (32') is formed in the sleeve (30") in a generally parallel relationship to the first circumferentially extending slot (32).
Abstract:
An improved method for predicting success of tumor therapies which relies on estimating the lowest effective concentration of a therapeutic agent that results in a majority of tumor cell kill in vitro is described. The method includes an assay and apparatus for simultaneously analyzing a series of dilutions of a therapeutic agent using 90 % cell kill as an index to effective cytotoxic concentration of the agent.
Abstract:
The present invention relates to an applied load accepting band (12, 12') for a centrifuge rotor (10) that is configured such that, while rotating, the applied loads on the band (12, 12') are balanced by tension in the band (12, 12'), so that during rotation the band (12, 12') is subjected to only a tensile force.
Abstract:
A swinging bucket rotor (R1) constructed of composite material having a central rotor body (B1) is disclosed. This central rotor body (B1) includes strap retaining surfaces (30, 31, 32). Wound endless composite fiber straps (S1, S2, S3, S4) having central portions (14) are attached to the respective top and bottom of the central rotor body (B1) and loop portions (16) remote from the central rotor body (B1) for mounting the swinging buckets (K1). These wound endless straps (S1, S2, S3, S4) attach centrally to the central rotor body (B1) at the strap retaining surfaces (30, 31, 32) and define bucket retaining loops (16) symmetrically spaced from the spin axis (20) of the rotor (R1). Each bucket retaining loop (16) holds spaced apart bushings (22) for receiving a trunion shaft (24). Paired trunions (24) on either side of a central sample tube receiving ring (26) are provided. The trunions (24) fit to the composite loop portions (16) of the endless composite material straps (S1, S2, S3, S4).
Abstract:
A fixed-angle centrifuge rotor (10) fabricated from fiber-reinforced composite material (11) including a composite rotor plate (12), composite tube holders (20), and a hub (16) to attach the rotor plate (12) to a centrifuge. The rotor plate (12) has counterbored through holes (18) with each counterbore defining an annular step (22). The tube holders (20) are cylindrical in shape and are mounted to the rotor plate (12) in each of the counterbored through holes (18). Each tube holder (20) has an circumferential flange that mates with and is bonded to the annular step (22) in a counterbore of the rotor plate (12).
Abstract:
A fixed-angle centrifuge rotor (10, 30, 61, 90) fabricated from fiber-reinforced composite material is disclosed that includes means for reinforcing radially outer portions (23, 78) of the cell holes (18, 58, 66, 76) in a direction transverse to the laminated layers (13, 70) of the rotor core (12, 32, 64, 92). In one embodiment, the reinforcement means is a material wound over the periphery of the rotor core (32, 92). In another embodiment, the reinforcement means is a material bonded into each of the cell holes (66). In a third embodiment, the reinforcement means is a formed region (74) of the laminated layers (70) to orient the fibers therein obliquely to the rotor axis (14).
Abstract:
An improved rotor suspension for a centrifuge (C) having a central stator (S) with a rotor (RC) is disclosed. The stator (S) is provided with a bore (114) coaxial to the axis of rotation of the rotor (RC). The rotor (RM) of the centrifuge motor has a quill shaft support member (118) extending into and rotating within the bore (114) of the stator (S). A quill shaft (Q) attaches at the bottom (120) of the quill shaft support member (118) and extends upward above the rotor (RM) of the centrifuge motor to support a fastening hub (H) for the centrifuge rotor (RC). The rotor (RC) of the centrifuge is detached from the rotor (RM) of the centrifuge motor so as to define a spatial interval between the supported spinning rotor (RC) and the rotor (RM) of the motor driving the centrifuge. The center of gravity of the centrifuge rotor (RC) is well below the hub (H) so that support of the rotor (RC) is stable. Rotor imbalances are absorbed by quill shaft flexure.