公开(公告)号：WO2007150047A1

公开(公告)日：2007-12-27

申请号：PCT/US2007/071938

申请日：2007-06-22

Applicant: MARTEK BIOSCIENCES CORPORATION ,  SUBRAMANIAN, Srinivasan ,  CONNOLLY, Brian, J. ,  HENDRICKSON, William, A.

Inventor： SUBRAMANIAN, Srinivasan ,  CONNOLLY, Brian, J. ,  HENDRICKSON, William, A.

IPC: A61K9/48

CPC classification number: A61K9/4891

Abstract: Products comprising labile compounds, such as polyunsaturated fatty acids, and having first and second encapsulates are disclosed. A first encapsulant can be a spray dried coating and the second encapsulant can be a prill coating. Methods of making the same are provided.

Abstract translation: 公开了包含不稳定化合物的产品，例如多不饱和脂肪酸，并且具有第一和第二封装物。 第一密封剂可以是喷雾干燥的涂层，第二密封剂可以是颗粒涂层。 提供了制备方法。

公开(公告)号：WO2007106905A2

公开(公告)日：2007-09-20

申请号：PCT/US2007/064106

申请日：2007-03-15

Applicant: MARTEK BIOSCIENCES CORPORATION ,  SEMBIOSYS GENETICS INC. ,  METZ, James, G. ,  KUNER, Jerry, M. ,  LIPPMEIER, James, Casey ,  MOLONEY, Maurice, Martin ,  NYKIFORUK, Cory, Lee

Inventor： METZ, James, G. ,  KUNER, Jerry, M. ,  LIPPMEIER, James, Casey ,  MOLONEY, Maurice, Martin ,  NYKIFORUK, Cory, Lee

IPC: A01H1/00 ,  C12N9/04 ,  C12N15/82 ,  C12N5/04

CPC classification number: C12N9/1029 ,  C12N9/93 ,  C12N15/8247 ,  C12P7/6427 ,  C12P7/6472 ,  Y02P20/52

Abstract: Disclosed are novel acyl-CoA synthetases and novel acyltransferases, nucleic acid molecules encoding the same, recombinant nucleic acid molecules and recombinant host cells comprising such nucleic acid molecules, genetically modified organisms (microorganisms and plants) comprising the same, and methods of making and using the same. Also disclosed are genetically modified organisms (e.g., plants, microorganisms) that have been genetically modified to express a PKS-like system for the production of PUFAs (a PUFA PKS system or PUFA synthase), wherein the organisms have been modified to express an acyl-CoA synthetase, to express an acyl transferase, to delete or inactivate a fatty acid synthase (FAS) expressed by the organism, to reduce competition for malonyl CoA with the PUFA synthase or to increase the level of malonyl CoA in the plant or plant cell, and in one aspect, to inhibit KASII or KASIII. Additional modifications, and methods to make and use such organisms, in addition to PUFAs and oils obtained from such organisms, are disclosed, alone with various products including such PUFAs and oils.

Abstract translation: 公开了新型酰基辅酶A合成酶和新型酰基转移酶，编码该酰基转移酶的核酸分子，重组核酸分子和包含该核酸分子的重组宿主细胞，包含该核酸分子的转基因生物（微生物和植物），以及制备和使用 一样。 还公开了经遗传修饰以表达用于生产PUFA（PUFA PKS系统或PUFA合酶）的PKS样系统的遗传修饰生物体（例如，植物，微生物），其中生物体被修饰以表达酰基 -CoA合成酶，以表达酰基转移酶，以删除或失活由生物表达的脂肪酸合成酶（FAS），以减少与PUFA合酶的丙二酰辅酶A竞争或增加植物或植物细胞中丙二酰辅酶A的水平 ，并且在一个方面，抑制KASII或KASIII。 除了PUFA和从这些生物获得的油之外，还公开了另外的修饰和制造和使用这些生物体的方法，其中包括各种产品，包括这种PUFA和油。

公开(公告)号：WO2007106903A2

公开(公告)日：2007-09-20

申请号：PCT/US2007/064104

申请日：2007-03-15

Applicant: MARTEK BIOSCIENCES CORPORATION ,  METZ, James G. ,  KUNER, Jerry M. ,  LIPPMEIER, James Casey

Inventor： METZ, James G. ,  KUNER, Jerry M. ,  LIPPMEIER, James Casey

IPC: A01H1/00 ,  C12N9/04 ,  C12N15/82 ,  C12N5/04

CPC classification number: C12N9/1029 ,  C12N9/93 ,  C12N15/8247 ,  C12P7/6427 ,  C12P7/6472 ,  Y02P20/52

Abstract: Disclosed are novel acyl-CoA synthetases and novel acyltransferases, nucleic acid molecules encoding the same, recombinant nucleic acid molecules and recombinant host cells comprising such nucleic acid molecules, genetically modified organisms (microorganisms and plants) comprising the same, and methods of making and using the same. Also disclosed are genetically modified organisms (e.g., plants, microorganisms) that have been genetically modified to express a PKS-like system for the production of PUFAs (a PUFA PKS system or PUFA synthase), wherein the organisms have been modified to express an acyl-CoA synthetase, to express an acyl transferase, to delete or inactivate a fatty acid synthase (FAS) expressed by the organism, to reduce competition for malonyl CoA with the PUFA synthase or to increase the level of malonyl CoA in the organism, and in one aspect, to inhibit KASII or KASIII. Additional modifications, and methods to make and use such organisms, in addition to PUFAs and oils obtained from such organisms, are disclosed, alone with various products including such PUFAs and oils.

Abstract translation: 公开了新型酰基辅酶A合成酶和新型酰基转移酶，编码该酰基转移酶的核酸分子，重组核酸分子和包含该核酸分子的重组宿主细胞，包含该核酸分子的转基因生物（微生物和植物），以及制备和使用 一样。 还公开了经遗传修饰以表达用于生产PUFA（PUFA PKS系统或PUFA合酶）的PKS样系统的遗传修饰生物体（例如，植物，微生物），其中生物体被修饰以表达酰基 -CoA合成酶，以表达酰基转移酶，以删除或失活由生物体表达的脂肪酸合成酶（FAS），以减少与PUFA合酶的丙二酰辅酶A的竞争或增加生物体中丙二酰辅酶A的水平，以及 一方面抑制KASII或KASIII。 除了PUFA和从这些生物获得的油之外，还公开了另外的修饰和制造和使用这些生物体的方法，其中包括各种产品，包括这种PUFA和油。

公开(公告)号：WO2006124598A2

公开(公告)日：2006-11-23

申请号：PCT/US2006/018445

申请日：2006-05-12

Applicant: MARTEK BIOSCIENCES CORPORATION ,  FICHTALI, Jaouad ,  NEEDHAM, Micah, Hazzy ,  LINSERT, Henry Jr.

Inventor： FICHTALI, Jaouad ,  NEEDHAM, Micah, Hazzy ,  LINSERT, Henry Jr.

IPC: A61K47/00 ,  A23J3/34 ,  C12P1/00

CPC classification number: C12P1/00 ,  A23J3/347 ,  A23L33/12 ,  A23L33/135 ,  A23L33/18 ,  A23V2002/00 ,  A61K8/361 ,  A61K8/97 ,  A61Q19/00 ,  C12P7/6427 ,  A23V2250/1882

Abstract: The present invention includes a palatable, stable composition comprising a biomass hydrolysate emulsion for incorporation, into, or used as, nutritional products, cosmetic products or pharmaceutical products. Preferred sources for biomass are microbial sources, plant sources and animal sources. The present invention also provides methods for making such compositions, specifically, a method for producing a product comprising a nutrient, particularly a long chain polyunsaturated fatty acid, comprising hydrolyzing a biomass comprising the nutrient and emulsifying the hydrolyzed biomass. Such compositions and methods are useful, for example, for increasing intake of nutrients such as omega-3 long chain polyunsaturated fatty acids having 18 or more carbons.

Abstract translation: 本发明包括一种可口的稳定的组合物，其包含用于掺入或用作营养产品，化妆品或药物产品的生物质水解产物乳液。 生物质的优选来源是微生物来源，植物来源和动物来源。 本发明还提供了制备这种组合物的方法，具体地说，涉及生产包含营养素，特别是长链多不饱和脂肪酸的产品的方法，包括水解包含营养物质的生物质并乳化水解的生物质。 这样的组合物和方法可用于例如增加摄入营养物质，例如具有18个或更多个碳的ω-3长链多不饱和脂肪酸。

公开(公告)号：WO2006055965A2

公开(公告)日：2006-05-26

申请号：PCT/US2005/042462

申请日：2005-11-21

Applicant: MARTEK BIOSCIENCES CORPORATION ,  ARTERBURN, Linda, Mary ,  BARCLAY, William ,  DANGI, Bindi ,  FLATT, James ,  LEE, Jung ,  VAN ELSWYK, Mary

Inventor： ARTERBURN, Linda, Mary ,  BARCLAY, William ,  DANGI, Bindi ,  FLATT, James ,  LEE, Jung ,  VAN ELSWYK, Mary

IPC: C07C57/00

CPC classification number: C12P7/6472 ,  C07C59/42 ,  C07D303/38 ,  C12P7/6427

Abstract: Disclosed are novel oxylipins, referred to herein as docosanoids, that are derived from C22 polyunsaturated fatty acids, and method of making and using such oxylipins. Also disclosed is the use of docosapentaenoic acid (C22:5n-6) (DPAn-6), docosapentaenoic acid (C22:5n-3) (DPAn-3), and docosatetraenoic acid (DTAn-6: C22:4n-6) as substrates for the production of novel oxylipins, and to the oxylipins produced thereby. Also disclosed is the use of DPAn-6, DPAn-3, DTAn-6, and/or the oxylipins derived therefrom, and/or novel docosanoids derived from the structures of C22 fatty acids, in therapeutic and nutritional or cosmetic applications, and particularly as anti-inflammatory or anti-neurodegenerative compounds. The invention also relates to novel ways of producing long chain polyunsaturated acid (LCPUFA)-rich oils and compositions that contain enhanced and effective amounts of LCPUFA-derived oxylipins, and particularly, docosanoids.

Abstract translation: 公开了衍生自C22多不饱和脂肪酸的新型oxylipin，本文称为二十二烷酸，以及制备和使用这种oxylipin的方法。 还公开了二十二碳五烯酸（C22：5n-6）（DPAn-6），二十二碳五烯酸（C22：5n-3）（DPAn-3）和二十二碳四烯酸（DTAn-6：C22：4n-6） 作为生产新型oxylipins的底物，以及由此产生的oxylipin。 还公开了在治疗和营养或化妆品应用中使用DPAn-6，DPAn-3，DTAn-6和/或衍生自其的oxylipin，和/或衍生自C22脂肪酸结构的新型二十二烷酸，特别是 作为抗炎或抗神经变性化合物。 本发明还涉及生产长链多不饱和酸（LCPUFA）的油和组合物的新方法，其含有增强和有效量的LCPUFA衍生的草甘膦脂类，特别是二十二烷酸。

公开(公告)号：WO2005079367A2

公开(公告)日：2005-09-01

申请号：PCT/US2005/004611

申请日：2005-02-14

Applicant: MARTEK BIOSCIENCES CORPORATION ,  METZ, James, G. ,  WEAVER, Craig, A. ,  KUNER, Jerry, M.

Inventor： METZ, James, G. ,  WEAVER, Craig, A. ,  KUNER, Jerry, M.

IPC: A61K31/202 ,  C07H21/04 ,  C12N1/10 ,  C12N9/10 ,  C12N9/80 ,  C12P7/64 ,  C12Q1/68

CPC classification number: C12N9/1029

Abstract: Disclosed are a fatty acid synthase (FAS) from Schizochytrium, biologically active fragments and homologues thereof, a nucleic acid sequence encoding such FAS, fragments and homologues thereof, the gene encoding Schizochytrium FAS, host cells and organisms that recombinantly express the FAS, host cells and organisms in which the expression and/or activity of the endogenous FAS has been attenuated, and various methods for making and using any of these proteins, nucleic acid molecules, genes, host cells or organisms.

Abstract translation: 公开了来自裂殖壶菌的脂肪酸合成酶（FAS），其生物活性片段及其同源物，编码这种FAS的核酸序列，其片段和同系物，编码裂殖酵母FAS的基因，重组表达FAS的宿主细胞和生物体宿主细胞 和其中内源性FAS的表达和/或活性已被减弱的生物体，以及用于制备和使用这些蛋白质，核酸分子，基因，宿主细胞或生物体中的任何一种的各种方法。

公开(公告)号：WO2005072477A3

公开(公告)日：2005-08-11

申请号：PCT/US2005/003614

申请日：2005-01-26

Applicant: MARTEK BIOSCIENCES CORPORATION ,  ABRIL, Jesus, Ruben

Inventor： ABRIL, Jesus, Ruben

IPC: C07C1/00

Abstract: Methods are disclosed for extracting and separating polar lipids, including phospholipids, from materials containing oil, polar lipids, protein, ash, and/or carbohydrate, such as egg yolks and other phospholipid-containing materials as depicted in Figure (1). In particular, methods for extracting phospholipids from phospholipid-containing materials through the use of an aliphatic alcohol and control of temperature are disclosed. Using these methods, phospholipids in the aqueous liquid fraction will be efficiently separated and will precipitate readily, and can be subjected to separation for improved purity.

公开(公告)号：WO2005035775A1

公开(公告)日：2005-04-21

申请号：PCT/US2004/032383

申请日：2004-10-01

Applicant: MARTEK BIOSCIENCES CORPORATION

Inventor： BEHRENS, Paul, W. ,  THOMPSON, John, M. ,  APT, Kirk ,  PFEIFER, Joseph, W., III ,  WYNN, James, P. ,  LIPPMEIER, James, Casey ,  FICHTALI, Jaouad ,  HANSEN, Jon

IPC: C12P7/40

CPC classification number: C12P7/6427 ,  A61K36/02 ,  C11B1/10 ,  C12P7/6409 ,  C12P7/6472

Abstract: Methods for production of highly unsaturated fatty acids by marine microorganisms, including the heterotrophic marine dinoflagellate Crypthecodinium, using low levels of chloride ion are disclosed. Specifically, methods of increasing production of highly unsaturated fatty acids by marine microorganisms while growing in low chloride media by manipulating sodium ion and potassium ion levels. The invention also relates to methods of production of highly unsaturated fatty acids by marine organisms at low pH levels, and includes methods for generation of low pH tolerant strains.

Abstract translation: 公开了使用低水平氯离子的海洋微生物生产高度不饱和脂肪酸的方法，包括异养海洋鞭毛藻Crypthecodinium。 具体地说，通过操纵钠离子和钾离子水平，在低氯化物培养基中生长时，通过海洋微生物增加高度不饱和脂肪酸的生产的方法。 本发明还涉及在低pH水平下由海洋生物生产高度不饱和脂肪酸的方法，并且包括产生低pH耐受菌株的方法。

公开(公告)号：WO1996040106A2

公开(公告)日：1996-12-19

申请号：PCT/US1996008649

申请日：1996-06-03

Applicant: MARTEK BIOSCIENCES CORPORATION ,  KYLE, David, J. ,  LINSERT, Henry, Jr.

Inventor： MARTEK BIOSCIENCES CORPORATION

IPC: A61K31/20

CPC classification number: A61K35/68 ,  A61K31/202 ,  A61K36/02 ,  A61K36/06

Abstract: A method of treating a neurological disorder comprises administering to a person affected from such a disorder a microbial oil comprising DHA, a microbial oil comprising ARA or a combination of DHA and ARA oils in an amount sufficient to elevate the levels of circulating DHA and/or ARA in the person's blood to at least normal levels.

Abstract translation: 一种治疗神经障碍的方法包括向患有这种病症的人施用包含DHA的微生物油，包含ARA或DHA和ARA油的组合的微生物油，其量足以提高循环DHA和/或 ARA在人的血液中至少达到正常水平。

公开(公告)号：WO1995021850A1

公开(公告)日：1995-08-17

申请号：PCT/US1995001389

申请日：1995-02-08

Applicant: MARTEK BIOSCIENCES CORPORATION

Inventor： MARTEK BIOSCIENCES CORPORATION ,  CHEN, Hao ,  RADMER, Richard, J.

IPC: C07H03/06

CPC classification number: C07H3/04 ,  B01J2219/00702 ,  B01J2219/00731 ,  C07H3/06 ,  C07H5/06 ,  C07H15/04 ,  C40B40/12

Abstract: Methods are provided for generating highly diverse mixtures of compounds which may be screened for biological activities. Once the activity is found, the component of the mixture which is responsible for the activity can be isolated by fractionation and assay for the biological activity. Polyhydroxylated organic monomers and oligomers are used as starting materials for generating the libraries.

Abstract translation: 提供了用于产生可被筛选用于生物活性的高度多样化合物混合物的方法。 一旦发现活性，负责活性的混合物的组分可以通过分离和测定生物活性来分离。 多羟基化有机单体和低聚物用作生成文库的起始材料。