公开(公告)号：EP0724730A1

公开(公告)日：1996-08-07

申请号：EP94930281.0

申请日：1994-10-20

Applicant: Cambridge Imaging Limited ,  PACKARD INSTRUMENT COMPANY

Inventor： RUSHBROOKE, John, Gordon ,  HOOPER, Claire, Elizabeth ,  NEALE, William, Wray ,  ANSORGE, Richard, Eric

IPC: G01T1

CPC classification number: G01T1/28 ,  G01T1/2928

Abstract: A method of detecting the presence and position of radio isotope material in a sample is described comprising the steps of: exposing the sample to a phosphor, repetitively imaging the phosphor onto an image intensified CCD camera, scanning the camera CCD array and interrogating the charge pattern thereon following each exposure, performing measurements on the data signals obtained thereby to identify clusters of data values from adjacent regions of the array caused by light emitted by the image intensifier onto those regions and comparing the measurements with threshold values and parameter values so as to distinguish clusters resulting from light emitted as a result of radioactive decay events energising the phosphor, from other light producing events, computing the centroid of each cluster of data values identified as a radioactive decay event with reference to the camera array and storing the centroid coordinates in a memory together with the centroid coordinates of any other radioactive decay produced clusters identified during the same interrogation. Coordinates from each of a succession of interrogations of the same sample are stored in an accumulation store, which can be read out to give a list of recorded events and/or used to produce a display of the events superimposed on an outline of the sample. The samples are carried on fibre optic plates in conctact with the photocathode and event coordinates are stored in memory as they are detected, so that the dispaly is updated as the events occur. Low noise bi-alkali material is used for the photocathode and the CCD camera is operated in inverted mode to reduce noise.

公开(公告)号：EP0691549A2

公开(公告)日：1996-01-10

申请号：EP95201510.5

申请日：1995-06-08

Applicant: Packard Instrument Company B.V.

Inventor： Thomson, James ,  Bösel, Herbert Manfred ,  Ter Wiel, Jan ,  Kremer, Gerhard Heinrich ,  Van Lune, Harry

IPC: G01T1/204 ,  B01J13/02 ,  C09K11/06

CPC classification number: G01T1/204

Abstract: A scintillation counting system utilizing scintillator capsules for the detection of radioactive substances in a sample for analysis include a liquid scintillator core encapsulated within a shell. The liquid scintillator comprises a base component or components and one or more fluors. The base component preferably includes one or more aromatic solvents. The fluors useful in the liquid or gel scintillator include any of the organic fluors well-known in the scintillation counting art. The liquid or gel scintillator can also include a wavelength shifter (i.e. secondary fluors). Useful wavelength shifters are also well known in the scintillation counting art. Additionally, a other materials can be added to the scintillator capsules to modify the characteristics of the scintillator capsules, and the scintillator capsules can be used in a variety of scintillation counting applications.

Abstract translation: 利用闪烁体胶囊检测样品中放射性物质进行分析的闪烁计数系统包括封装在壳内的液体闪烁体核。 液体闪烁体包含基础组分或一种或多种荧光。 基础组分优选包括一种或多种芳族溶剂。 在液体或凝胶闪烁体中有用的荧光剂包括在闪烁计数领域中众所周知的任何有机荧光。 液体或凝胶闪烁体还可以包括波长移位器（即次级荧光）。 有用的波长移位器在闪烁计数技术中也是众所周知的。 此外，可以将其它材料加入到闪烁体胶囊中以改变闪烁体胶囊的特性，并且闪烁体胶囊可用于各种闪烁计数应用。 $$

公开(公告)号：EP0645187A3

公开(公告)日：1995-10-25

申请号：EP94111503.2

申请日：1994-07-22

Applicant: PACKARD INSTRUMENT COMPANY, INC.

Inventor： Kolb, Alfred J. ,  Manns, Roy L. ,  Neumann, Kenneth E.

IPC: B01L3/00 ,  G01T1/204 ,  G01N21/76 ,  G01T7/02

CPC classification number: B01L3/50255 ,  G01T1/204 ,  G01T7/02 ,  Y10S435/809

Abstract: A microplate assembly (10) for use in analyzing samples captured on a filter medium (16) comprises a carrier plate (12), a holding tray (14), a collimator (18), and a cover film (20). These elements are generally rectangular in shape and are sized to stack on top of one another. The holding tray (14) is positioned within the carrier plate (12), the collimator (18) and filter medium (16) are positioned within the holding tray (14) with the filter medium (16) positioned beneath the collimator (18), and the cover film (20) is sealed over the collimator (18). To prepare samples in the microplate assembly (10) for analysis, the samples are captured on the filter medium (16) and the filter medium (16) is placed in the holding tray (14). After adding scintillation cocktail or luminescent substrate to the filter medium (16), the collimator (18) is placed over the holding tray (14) with the filter medium (16) positioned between the collimator (18) and the holding tray (14) and the samples disposed in the sample wells. The carrier plate (12), the holding tray (14), the filter medium (16), and the collimator (18) are provided with complementary keyed corners (22,34,19,46) to facilitate alignment of these elements relative to one another. The wells of the collimator (18) include respective lower rims (54) protruding into the filter medium (16) to minimize crosstalk through the filter medium (16). The cover film (20) seals the microplate assembly (10) so that the samples are prepared for analysis.

公开(公告)号：EP0425767B1

公开(公告)日：1994-03-30

申请号：EP90114090.5

申请日：1990-07-23

Applicant: Packard Instrument Company, Inc.

Inventor： Van Cauter, Gustaaf C. ,  Osten, Donald E. ,  Tomisek, John D.

IPC: G01T1/29

CPC classification number: G01T1/204 ,  G01T1/2914 ,  G01T7/08

公开(公告)号：EP0571661A1

公开(公告)日：1993-12-01

申请号：EP92119631.7

申请日：1992-11-17

Applicant: Packard Instrument Company, Inc.

Inventor： Manns, Roy L. ,  Kolb, Alfred J. ,  Effertz, Bernard S.

IPC: B01L3/00 ,  G01N21/03

CPC classification number: B01L3/5085 ,  B29C45/16 ,  G01N21/03 ,  G01N2021/0346

Abstract: A microplate (10) forms a multiplicity of sample wells for holding samples to be assayed by light emissions or light transmission. The plate (10) comprises an upper plate (11) forming the side walls (13) of the sample wells, the side walls (13) being opaque so that light cannot be transmitted between adjacent wells through the side walls, (13) and a lower plate (12) forming the bottom walls (14) of the sample wells, the bottom walls (14) being transparent to allow the transmission of light therethrough. Bands of opaque material are provided within the lower plate (12) and surrounding each well to block the transmission of light between adjacent wells through the lower plate (12).

Abstract translation: 微板（10）形成多个样品孔，用于保持通过光发射或光透射测定的样品。 板（10）包括形成样品孔的侧壁（13）的上板（11），侧壁（13）是不透明的，使得光不能通过侧壁（13）和 形成样品井的底壁（14）的下板（12），底壁（14）是透明的，以允许光透过。 不透明材料的带设置在下板（12）内并且围绕每个井以阻挡相邻井之间通过下板（12）的透射。

公开(公告)号：EP0502123A4

公开(公告)日：1992-11-04

申请号：EP91901232

申请日：1990-11-14

Applicant: PACKARD INSTRUMENT COMPANY, INC.

Inventor： VAN CAUTER, GUSTAAF, C. ,  OSTEN, DONALD, E. ,  NEUMANN, KENNETH, E.

IPC: G01N33/542 ,  G01N33/552 ,  G01N33/60 ,  G01N33/573 ,  C12Q1/68

CPC classification number: G01N33/60 ,  G01N33/542 ,  G01N33/552

Abstract: A new support body (10) for use in a scintillation proximity radioimmunoassay consisting of a scintillation material. The support body (10) of the present invention permits extremely sensitive and specific assays for a broad range of biological and non-biological substances. The support body (10) of the present invention having a ligand (16) coupled to its surface which is capable of selectively biochemically binding to a reactant of interest (11) resulting in a complex (18).

公开(公告)号：EP0425767A1

公开(公告)日：1991-05-08

申请号：EP90114090.5

申请日：1990-07-23

Applicant: Packard Instrument Company, Inc.

Inventor： Van Cauter, Gustaaf C. ,  Osten, Donald E. ,  Tomisek, John D.

IPC: G01T1/29

CPC classification number: G01T1/204 ,  G01T1/2914 ,  G01T7/08

Abstract: A system for in-situ measurement of the radioactivity of multiple samples while the samples are in a multiple-well sample tray, with a scintillator in each sample-containing well for producing light pulses in response to radiation from the respective samples. Multiple photomultiplier tubes are positioned adjacent to the sample wells containing the scintillator for simultaneously measuring the radioactivity of multiple samples with only a single photomultiplier tube sensing the scintillations from each well and converting the sensed scintillations into corresponding electrical pulses. The electrical pulses from each photomultiplier tube are processed to discriminate between pulses attributable to sample events within the wells and pulses attributable to non-­sample events such as photomultiplier tube noise. The discrimination is effected by determining whether a selected number of electrical pulses occurs within a prescribed time interval, the occurrence of the selected number of pulses within the prescribed time interval signifying a sample event. Only the electrical pulses attributable to sample events are supplied to a pulse analyzer.

Abstract translation: 一种用于在多个样品的放射性的现场测量，而样品在多阱​​样品盘，具有良好用于响应于辐射从样品respectivement产生光脉冲的每个样品含有在闪烁体系统。 多个光电倍增管被定位成邻近所述样品井含用于从每个同时测量多个样品的放射活性仅具有单个光电倍增管感测火花和灰孔和将所述感测到的火花和灰为相应的电脉冲的闪烁体。 从各光电倍增管的电脉冲处理，以区分之间的脉冲属性能够在井内采样的事件和属性的脉冲能够非样品事件：例如光电倍增管的噪声。 鉴别是通过确定性采矿实现无论所选数量的电脉冲的一个规定的时间间隔内发生，在规定的时间间隔标志着一个示例事件内的脉冲的选定数目的发生。 只有电脉冲属性能够采样事件被提供给一个脉冲分析仪。

公开(公告)号：EP0202185B1

公开(公告)日：1990-09-05

申请号：EP86630051.0

申请日：1986-04-03

Applicant: Packard Instrument Company, Inc.

Inventor： van Cauter, Gustav C. ,  Everett, Leroy J. ,  DeFilippis, Stanley J.

IPC: G01T1/204

CPC classification number: G01T1/2045 ,  G01T1/204

公开(公告)号：EP0199665B1

公开(公告)日：1990-07-18

申请号：EP86630052.8

申请日：1986-04-03

Applicant: Packard Instrument Company, Inc.

Inventor： Jones, Donald K. ,  Tomisek, John David ,  Young, Harry Major

IPC: G01T1/204

CPC classification number: G01T1/204

公开(公告)号：EP0202185A1

公开(公告)日：1986-11-20

申请号：EP86630051.0

申请日：1986-04-03

Applicant: Packard Instrument Company, Inc.

Inventor： van Cauter, Gustav C. ,  Everett, Leroy J. ,  DeFilippis, Stanley J.

IPC: G01T1/204

CPC classification number: G01T1/2045 ,  G01T1/204

Abstract: Apparatus and a regionless method of ascertaining the activity of each radionuclide in a dual-label sample are disclosed. A set of correlation curves is generated utilizing quench standards for both a low energy radionuclide and a high energy radionuclide. A test sample was then counted and the quench-indicating parameters for the test sample are compared with the quench-indicating parameters of the correlation curves to determine the appropriate contribution of each radionuclide to the overall energy spectra of the test sample. From this comparison the overall count rate for the test sample may be allocated between a low energy radionuclide and a high energy radionuclide. By dividing the count rates by efficiencies determined from another set of correlation curves the actual disintegration per minute (DPM) rate for each radionuclide may be ascertained.