公开(公告)号：JP2006304707A

公开(公告)日：2006-11-09

申请号：JP2005132379

申请日：2005-04-28

Applicant: Sony Corp ,  ソニー株式会社

Inventor： NEGISHI MAKIKO ,  MAMINE TAKAYOSHI

IPC: C12Q1/68 ,  C12N15/09 ,  G01N33/53 ,  G01N33/566

Abstract: PROBLEM TO BE SOLVED: To provide a bioassay method for preferentially advancing normal hybridization between a probe nucleic acid chain and a target nucleic acid chain to enhance the accuracy of hybridization detection.
SOLUTION: This bioassay method is characterized by adding a substance interacting with a nucleic acid chain, such as an intercalator, to a reaction region in a system containing in a mixed state a normal target nucleic acid chain having a base sequence or base sequence portion capable of forming a perfect complementary chain together with a probe nucleic acid chain existing in the reaction region formed on a substrate or the like and an error target nucleic acid chain having a base sequence or base sequence portion capable of forming a mismatch base pair together with the above-mentioned probe nucleic acid chain, thereby more preferentially advancing normal hybridization between the probe nucleic acid chain and the target nucleic acid chain than miss hybridization between the probe nucleic acid chain and the error nucleic acid chain to improve the hybridization detection.
COPYRIGHT: (C)2007,JPO&INPIT

Abstract translation: 要解决的问题：提供优先推进探针核酸链和靶核酸链之间的正常杂交的生物测定方法，以提高杂交检测的准确性。 解决方案：该生物测定方法的特征在于将与核酸链相互作用的物质（例如嵌入剂）加入含有混合状态的系统中的反应区域，所述正常靶核酸链具有碱基序列或碱基 能够与形成在底物等上的反应区域中存在的探针核酸链一起形成完全互补链的序列部分和具有能够形成错配碱基对的碱基序列或碱基序列部分的错误靶核酸链 与上述探针核酸链一起，从而更优先地推进探针核酸链和靶核酸链之间的正常杂交，而不是探针核酸链和错误核酸链之间的错过杂交，以改进杂交检测。 版权所有（C）2007，JPO＆INPIT

公开(公告)号：JP2006003148A

公开(公告)日：2006-01-05

申请号：JP2004178104

申请日：2004-06-16

Applicant: Sony Corp ,  ソニー株式会社

Inventor： TAKEDA MINORU ,  FURUKI MOTOHIRO ,  DATE TATSUMI ,  AKI YUICHI ,  TAKASHIMIZU TORU ,  MAMINE TAKAYOSHI

IPC: G01N33/53 ,  C12M1/00 ,  C12M1/34 ,  C12N15/09 ,  G01N37/00

Abstract: PROBLEM TO BE SOLVED: To speed up interaction in a channel part that is a field of the interaction between substances. SOLUTION: This detection part has: a reservoir part R storing or holding a medium M; the channel part C that is the field between the substances progressing inside the medium sent from the reservoir part R, communicating with the reservoir part R; an outside air communication part A communicating with the channel part C; and counter electrodes E 1 -E 2 oppositely disposed such that the counter electrodes E 1 -E 2 face the channel part C, capable of applying an electric field to the medium M inside the channel part C. COPYRIGHT: (C)2006,JPO&NCIPI

Abstract translation: 要解决的问题：加速作为物质之间相互作用领域的通道部分的相互作用。

解决方案：该检测部具有：存储或保持介质M的储存部R; 通道部分C是从与储存部分R连通的从储存器部分R发送的介质内的物质之间的场; 与通道部C连通的外部空气连通部A; 和相对设置的相对电极E -E 2 使得相对电极E -E 2 面向 通道部分C，能够向通道部分C内部的介质M施加电场。C.版权所有（C）2006，JPO＆NCIPI

公开(公告)号：JP2005117917A

公开(公告)日：2005-05-12

申请号：JP2003354283

申请日：2003-10-14

Applicant: Sony Corp ,  ソニー株式会社

Inventor： MAMINE TAKAYOSHI ,  YUBI HIROSHI

IPC: C12M1/00 ,  C12Q1/68

Abstract: PROBLEM TO BE SOLVED: To detect the base sequence of a DNA at a high speed. SOLUTION: A DNA sequencer 10 is equipped with a solution reserving part 12 for reserving a solution 13 in which an ssDNA (a single-stranded DNA) 1 is suspended, a cylindrical part 15 formed in the interior of the solution reserving part 12 and composed of, e.g. a silicon nanowire, a pair of sensors 21 and 22 formed in the vicinity of an opening of the cylindrical part 15 and a pair of electrodes 25 and 26 for applying an AC electric field into the solution 13. In the DNA sequencer 10, the AC electric field is applied into the solution 13 to extend the DNA 1 and make the DNA 1 migrate into the silicon nanowire by electrophoresis. An electric change is detected with the sensors 21 and 22 when the DNA 1 passes through the interior of the silicon nanowire. The base sequence of the DNA 1 is detected on the basis of the change. COPYRIGHT: (C)2005,JPO&NCIPI

Abstract translation: 要解决的问题：高速检测DNA的碱基序列。 解决方案：DNA测序仪10配备有用于保留其中悬浮有ssDNA（单链DNA）1的溶液13的溶液保留部分12，形成在溶液保留部分的内部的圆柱形部分15 12，由...组成 硅纳米线，形成在圆筒部15的开口附近的一对传感器21和22以及用于将AC电场施加到溶液13中的一对电极25和26.在DNA定序器10中，AC 将电场施加到溶液13中以延伸DNA 1，并使DNA 1通过电泳迁移到硅纳米线中。 当DNA 1穿过硅纳米线的内部时，传感器21和22检测到电变化。 基于变化检测DNA 1的碱基序列。 版权所有（C）2005，JPO＆NCIPI

公开(公告)号：JP2005030784A

公开(公告)日：2005-02-03

申请号：JP2003193065

申请日：2003-07-07

Applicant: SONY CORP

Inventor： TAKEDA MINORU ,  MAMINE TAKAYOSHI ,  NAKAO ISAMU ,  FURUKI MOTOHIRO ,  YAMAMOTO MASANOBU

IPC: G01N33/53 ,  C12M1/00 ,  C12M1/34 ,  C12N15/09 ,  C12Q1/68 ,  G01N35/02 ,  G01N35/04 ,  G01N35/10 ,  G01N37/00

Abstract: PROBLEM TO BE SOLVED: To perform hybridization at high speed. SOLUTION: As for a substrate 1 for bioassay, the main surface similar to an optical disk such as a CD has an annular flat shape. The substrate 1 is driven rotatively on a center hole 2. The substrate 1 has the surface 1a on which a plurality of wells 8 are formed, which are fields for a hybridization reaction between a probe DNA and a sample DNA. A transparent electrode film 4 is formed on the lower layer of the wells 8 on the substrate 1. At the hybridization time, an external electrode 18 is brought close from the upper surface 1a side of the substrate 1, and an alternating-current power is applied between the transparent electrode film 4 and the external electrode 18, to thereby apply an alternating-current field in the vertical direction to the substrate 1. An application method of the field to the transparent electrode film 4 is performed from a chucking mechanism through the center hole. COPYRIGHT: (C)2005,JPO&NCIPI

公开(公告)号：JP2004125706A

公开(公告)日：2004-04-22

申请号：JP2002292861

申请日：2002-10-04

Applicant: Sony Corp ,  ソニー株式会社

Inventor： MAMINE TAKAYOSHI ,  KINOSHITA TAKASHI

IPC: C12M1/00 ,  C12N15/09 ,  C12Q1/68 ,  G01N27/00 ,  G01N33/53 ,  G01N37/00 ,  G01Q10/00 ,  G01Q60/24 ,  G01Q80/00 ,  G01N13/16

CPC classification number: G01N29/036 ,  B82Y5/00 ,  C12Q1/6816 ,  G01N2291/0257 ,  C12Q2565/60

Abstract: PROBLEM TO BE SOLVED: To detect the hybridization and the intereaction between objects with a fixed amount.
SOLUTION: In a cell detector 2, surface processing is applied to an end surface 13a of a cantilever 13, and nucleotide tether D for detection can be fixed thereto. A positive electrode 11 and a negative electrode 12 generate an electric field in a reactive region 10. A target nucleotide tether T deposited from a nozzle 3 moves to the end surface 13a while being extended. Hybridization of the detected nucleotide tether D and the target nucleotide tether T causes the mass of the cantilever 13 to increase, and the number of fixed vibrations decreases. The change in the fixed vibrations is measured by applying an AC voltage to the cantilever 13. In this way, the hybridization can be detected and the number of the target nucleotide tether T being hybridized is detected.
COPYRIGHT: (C)2004,JPO

公开(公告)号：JP2004045376A

公开(公告)日：2004-02-12

申请号：JP2003105704

申请日：2003-04-09

Applicant: Sony Corp ,  ソニー株式会社

Inventor： MAMINE TAKAYOSHI

IPC: G01N33/53 ,  C12M1/00 ,  G01N33/566 ,  G01N37/00

Abstract: PROBLEM TO BE SOLVED: To provide a substrate for bioassay having high accuracy of a hybridization reaction.
SOLUTION: This substrate 1(4) for bioassay is equipped with at least a detection surface 21(51) to which surface processing is applied, capable of immobilizing a terminal portion of a nucleotide chain D for detection on a disc-shaped substrate capable of optically reading record information, positive and negative electrodes 22(52) for forming an electric field for elongating the nucleotide chain D for detection in the immobilized state on the detection surface 21(51), and a reaction area 23(53) which is a field for the hybridization reaction between the nucleotide chain D for detection and a target nucleotide chain T.
COPYRIGHT: (C)2004,JPO

公开(公告)号：JP2002139758A

公开(公告)日：2002-05-17

申请号：JP2000332580

申请日：2000-10-31

Applicant: SONY CORP ,  NAT INST OF ADV IND & TECHNOL

Inventor： HANE HIROKI ,  MAMINE TAKAYOSHI ,  KANEDA YUJI ,  OGAKI HIDEAKI ,  TOYOKAWA HIROYUKI ,  MISUMI TOMOHISA

IPC: G21K5/02 ,  G02F2/02 ,  G03F7/20 ,  H01L21/027 ,  H01S3/30

Abstract: PROBLEM TO BE SOLVED: To provide a device for shortening light wavelength in which the variation of wavelength of light does not occur even if energy of an electron beam from an electron beam source is varied, or/also, a shortened wavelength of light can be easily changed. SOLUTION: This device is provided with an electron beam source 10, a laser beam source 20, and a vacuum chamber 30, electrons in an electron beam which are emitted from the electron beam source 10 and moving at relativistic speed is collided with photons in a laser beam emitted from the laser beam source 20 in the vacuum chamber 30, energy of electrons is given to photons of the laser beam. A device for shortening light wavelength to shorten scattered light wavelength is provided with angle varying means 21, 22 for changing a collision angle of the laser beam and the electron beam before collision of electrons and photons, in order to change wavelength of scattered light.

公开(公告)号：JP2000294523A

公开(公告)日：2000-10-20

申请号：JP9497399

申请日：1999-04-01

Applicant: SONY CORP

Inventor： MAMINE TAKAYOSHI ,  MATSUZAWA NOBUYUKI ,  KISHII NORIYUKI

IPC: H01L21/302 ,  G03F7/20 ,  H01L21/027

Abstract: PROBLEM TO BE SOLVED: To sufficiently form precise patterns by an ablation by a method wherein lights irradiated on a wafer surface collide with electrons in electron beams and photons of lights of a longer wavelength, and electronic energy is caused by inverse Compton scattering lights given to the above photons. SOLUTION: In semiconductor manufacturing apparatus, a material of an exposure part is removed by utilizing a gassing reaction (ablation) of a material by optical irradiation to form patterns on a processing face. Here, as a light source, electron beams collide with laser beams, and shortwave long lights generated due to inverse Compton scattering effects are used. From the light sources of the shortwave long lights, lights are incident on reflecting means composed of at least a pair of concave mirrors, and electron beams 4 are incident on, preferably, laser beams 3 which are focused and reciprocally reflected by the reflecting means. The electron beams 4 are incident thereon, whereby lights 3 collide with the electron beams 4 at high density in a scattering region 5, thereby obtaining scattering lights 6 (for example, X rays).

公开(公告)号：JPH11211899A

公开(公告)日：1999-08-06

申请号：JP7364998

申请日：1998-03-23

Applicant: SONY CORP

Inventor： MAMINE TAKAYOSHI

IPC: H01L21/027 ,  G03F7/20 ,  G21K5/02 ,  H05G2/00

Abstract: PROBLEM TO BE SOLVED: To let collide an electronic beam to light in high density at many times to increase the collecting quantity of scattered light by making light generated by a light emitting means incident on a reflecting means, and when the light is converged and repeatedly reflected, making the electronic beam incident toward the converged and repeatedly reflected light from an electronic beam generating means. SOLUTION: Laser beams L are emitted from a laser beam source 2, and made incident between a pair of concave mirrors M10, M20. The laser beams L repeat convergence and reflection between the concave mirrors M10, M20 according to the radius of curvature. Next an electronic beam is emitted from an electronic beam generating part 3 and made collide with the laser beams L. The electronic beam e collides with the converged and repeatedly reflected laser beams L at very high frequency. Because the laser beams L are converged and repeatedly reflected between the concave mirrors M10, M20, the phonton density of the convergent part is highly raised, and by making the electronic beam incident thereon, very much anti-Compton scattering is caused in the scattering territory S. Therefore, the scattered light can be generated in a large collecting quantity.

公开(公告)号：JPH10326928A

公开(公告)日：1998-12-08

申请号：JP13480697

申请日：1997-05-26

Applicant: AGENCY IND SCIENCE TECHN ,  SONY CORP ,  OGAKI HIDEAKI

Inventor： OGAKI HIDEAKI ,  MAMINE TAKAYOSHI

IPC: G21K1/00 ,  H01S3/0959

Abstract: PROBLEM TO BE SOLVED: To provide a light wavelength shortening device, capable of obtaining more than 10 light quanta of which wavelength is shortened due to a collision between an electron beam and a laser beam and easily obtaining an electromagnetic wave (ultraviolet rays, X-rays, etc.) having required wavelengths. SOLUTION: This light wavelength shortening device is provided with an electron beam source, a laser light source 20 and an optical resonator 22 and constituted so that the light quanta in a laser beam projected from the light source 20 and propagated into the resonator 22 and electrons in an electron beam projected from the electron beam source and acting at a relativistic speed are mutually collided plural times, and energy included in the electrons are applied to quanta in the laser beam to shorten the wavelength of the scattered light.