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    HEATING ELEMENT FOR A ROTATING MULTIPLEX FLUORESCENCE DETECTION DEVICE
    81.
    发明申请
    HEATING ELEMENT FOR A ROTATING MULTIPLEX FLUORESCENCE DETECTION DEVICE 审中-公开
    旋转多重荧光检测装置的加热元件

    公开(公告)号：WO2007005077A1

    公开(公告)日：2007-01-11

    申请号：PCT/US2006/010819

    申请日：2006-03-24

    Applicant: 3M INNOVATIVE PROPERTIES COMPANY

    Inventor： BEDINGHAM, William D., ,  LUDOWISE, Peter D., ,  ROBOLE, Barry W.,

    IPC: B01L7/00

    CPC classification number: G01N21/645 ,  B01L3/5027 ,  B01L7/52 ,  B01L7/54 ,  B01L2200/147 ,  B01L2300/0806 ,  B01L2300/1805 ,  B01L2300/1844 ,  B01L2300/1872 ,  G01N21/07 ,  G01N21/6428 ,  G01N2021/6419 ,  G01N2021/6421 ,  G01N2201/04 ,  G01N2201/0407 ,  G01N2201/08 ,  G01N2201/10 ,  G01N2201/101

    Abstract: Techniques are described for the detection of multiple target species in real-time PCR (polymerase chain reaction). For example, a system comprises a data acquisition device and a detection device coupled to the data acquisition device. The detection device includes a rotating disk having a plurality of process chambers having a plurality of species that emit fluorescent light at different wavelengths. The device further includes a plurality of removable optical modules that are optically configured to excite the species and capture fluorescent light emitted by the species at different wavelengths. A fiber optic bundle coupled to the plurality of removable optical modules conveys the fluorescent light from the optical modules to a single detector. The device further includes a heating element for heating one or more process chambers on the disk. In addition, the device may control the flow of fluid in the disk by locating and selectively opening valves separating chambers by heating the valves with a laser.

    Abstract translation: 描述了用于在实时PCR（聚合酶链式反应）中检测多种靶物种的技术。 例如，系统包括数据采集装置和耦合到数据采集装置的检测装置。 检测装置包括具有多个处理室的旋转盘，该多个处理室具有发射不同波长的荧光的多种物质。 该装置还包括多个可移除的光学模块，其被光学配置为激发物种并捕获由不同波长的物质发射的荧光。 耦合到多个可移除光学模块的光纤束将来自光学模块的荧光传送到单个检测器。 该装置还包括用于加热盘上的一个或多个处理室的加热元件。 此外，该装置可以通过利用激光加热阀来定位和选择性地打开阀分离腔来控制盘中的流体流动。

    PORTABLE AND CARTRIDGE-BASED SURFACE PLASMON RESONANCE SENSING SYSTEMS
    82.
    发明申请
    PORTABLE AND CARTRIDGE-BASED SURFACE PLASMON RESONANCE SENSING SYSTEMS 审中-公开
    基于便携式和基于盒式的表面质谱共振感应系统

    公开(公告)号：WO2006113177A2

    公开(公告)日：2006-10-26

    申请号：PCT/US2006/013080

    申请日：2006-04-06

    Applicant: UNIVERSITY OF WASHINGTON ,  CHINOWSKY, Timothy, M. ,  SOELBERG, Scott, D. ,  KAUFFMAN, Peter, C. ,  FURLONG, Clement, E. ,  TRITZ, Jared ,  GROW, Michael, S. ,  NAIMUSHIN, Alexei, N. ,  YEE, Sinclair, S.

    Inventor： CHINOWSKY, Timothy, M. ,  SOELBERG, Scott, D. ,  KAUFFMAN, Peter, C. ,  FURLONG, Clement, E. ,  TRITZ, Jared ,  GROW, Michael, S. ,  NAIMUSHIN, Alexei, N. ,  YEE, Sinclair, S.

    IPC: G01N21/55

    CPC classification number: G01N21/553 ,  G01N2201/04

    Abstract: This specification discloses various improvements in the field of SPR sensing systems. One improvement relates to a portable SPR sensing system, e.g., a system contained within a suitcase that can be hand-carried to a monitoring site. Another improvement relates to a portable, cartridge-based SPR sensing system. In this system, selected portions of the system's electrical and fluidics systems are allocated between a base unit and a removable/disposable cartridge. Other improvements relate to methods or protocols for operating an SPR sensing system. Such methods provide for the elimination of false positives and increased sensitivity, e.g., by using secondary antibodies with specificity for different target epitopes and by sensor element redundancy. In addition, protocols are provided for the detection of small molecules. Such protocols may employ a competition type assay where the presence of the analyte inhibits the binding of antibodies to surface immobilized analyte, or a displacement assay, where antibodies bound to the analyte on the sensor surface are displaced by free analyte.

    Abstract translation: 本说明书公开了SPR传感系统领域的各种改进。 一个改进涉及便携式SPR感测系统，例如，可以手提携带到监控地点的手提箱内的系统。 另一项改进涉及一种便携式，基于盒式SPR感测系统。 在该系统中，系统的电和流体系统的选定部分被分配在基本单元和可移动/一次性盒之间。 其他改进涉及用于操作SPR感测系统的方法或协议。 这样的方法提供消除假阳性和提高灵敏度，例如通过使用对不同靶标表位具有特异性的二级抗体以及通过传感器元件冗余。 此外，提供了用于检测小分子的方案。 这样的方案可以采用竞争型测定法，其中分析物的存在抑制抗体与表面固定化分析物的结合，或位移测定法，其中与传感器表面上的分析物结合的抗体被游离分析物置换。

    Methods and systems for pure dye instrument normalization
    85.
    发明授权
    Methods and systems for pure dye instrument normalization 有权

    公开(公告)号：US09809849B2

    公开(公告)日：2017-11-07

    申请号：US15016713

    申请日：2016-02-05

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Jeffrey Marks

    IPC: G01N21/64 ,  C12Q1/68 ,  G01N21/27

    CPC classification number: C12Q1/686 ,  G01N21/274 ,  G01N21/6428 ,  G01N21/6452 ,  G01N2021/6421 ,  G01N2201/04 ,  G01N2201/068 ,  G01N2201/12746

    Abstract: The present teachings relate to a method and system for normalizing spectra across multiple instruments. In an embodiment of the present invention, the method comprises at least one reference instrument and a test instrument. Each instrument comprises at least one excitation filter and at least one emission filter arranged in pairs. Each instrument further comprises a pure dye plate comprising a plurality of wells. Each well contains a plurality of dyes where each dye comprises a fluorescent component. Fluorescent spectra are obtained from each instrument for each dye across multiple filter combinations to contribute to a pure dye matrix Mref for the reference instrument and pure dye matrix M for the test instrument. The pure dye spectra can then be multiplied by correction factors for each filter pair to result in corrected pure dye spectra, then normalized and the multicomponenting data can be extracted.

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