公开(公告)号：US5084246A

公开(公告)日：1992-01-28

申请号：US923906

申请日：1986-10-28

Applicant: George Lyman ,  Anthony Labriola

Inventor： George Lyman ,  Anthony Labriola

IPC: B01L3/00 ,  G01N21/03

CPC classification number: B01L3/50855 ,  B01L3/50255 ,  B01L3/5085 ,  G01N21/03 ,  G01N2021/0357 ,  G01N2201/0446 ,  Y10S435/809 ,  Y10S436/809 ,  Y10T436/25375

Abstract: A multi-well test plate which includes a base and a plurality of well strips. The well strips are removable from the base and the individual wells of each strip are separable. The wells in each strip are joined by T-shaped connecting members which hold the wells in a flat linear array when the well strips are either held in or removed from the base and the T-shaped members are readily severable to permit easy separation of individual wells.

Abstract translation: 一种多井测试板，其包括基座和多个井条。 井条可从基座移除，每个条带的各个井可分离。 每个条带中的孔由T形连接构件连接，当井条被保持在基座中或从基座移除时，T形连接构件将井保持在平坦的线性阵列中，并且T形构件易于分离以允许容易地分离个体 井。

公开(公告)号：US4741619A

公开(公告)日：1988-05-03

申请号：US47037

申请日：1987-05-05

Applicant: Gillian M. K. Humphries ,  Viola T. Kung

Inventor： Gillian M. K. Humphries ,  Viola T. Kung

IPC: B01L3/00 ,  B29C59/14 ,  G01N21/03

CPC classification number: B01L3/5085 ,  B29C59/14 ,  G01N21/03 ,  G01N2201/0446

Abstract: A microtiter plate prepared from a hydrophobic plastic is rendered more hydrophilic by treatment of the surfaces of the wells of the microplate prior to use. The resulting plates are particularly useful in vertical beam photometry.

公开(公告)号：US4710031A

公开(公告)日：1987-12-01

申请号：US761199

申请日：1985-07-31

Applicant: John E. Kelly ,  Don C. Jones ,  Frederick R. Tuck ,  William A. Zimmermann ,  Kenneth R. Clark

Inventor： John E. Kelly ,  Don C. Jones ,  Frederick R. Tuck ,  William A. Zimmermann ,  Kenneth R. Clark

IPC: G01N21/29 ,  G01N21/00

CPC classification number: G01N21/253 ,  G01N21/29 ,  G01N2201/0446 ,  G01N2201/06126

Abstract: A microtiter plate reader is disclosed which allows visual examination of the contents of the wells of a microtiter plate having an array of wells. The microtiter plate reader includes supporting means for supporting the microtiter plate with the wells opening generally upwardly and a light emitting surface is adapted to extend under a microtiter plate so held. Regions of reduced light emission on the light emitting surface are arranged in an array corresponding in relative position to a selected portion of the array of microtiter plate wells. Locator means are provided for locating the microtiter plate with respect to the array of regions of reduced light emission to allow selective alignment of the wells with the dark regions, providing a dark background to the bottom of the wells which are then illuminated indirectly. The wells can be illuminated directly when the dark regions are out of alignment with the wells.

Abstract translation: 公开了一种微量滴定板读数器，其允许目视检查具有孔阵列的微量滴定板的孔的内容物。 微量滴定板读取器包括用于支撑微量滴定板的支撑装置，其中孔通常向上开口，并且发光表面适于在如此保持的微量滴定板下方延伸。 在发光表面上减少发光的区域以对应于微量滴定板孔阵列的选定部分的相对位置的阵列排列。 提供了定位装置，用于相对于减少发光的区域阵列来定位微量滴定板，以允许孔与暗区域的选择性对准，从而为井的底部间接提供了深色背景。 当暗区与井不对齐时，井可以直接照明。

公开(公告)号：US4501970A

公开(公告)日：1985-02-26

申请号：US433825

申请日：1982-10-12

Applicant: Keith E. Nelson

Inventor： Keith E. Nelson

IPC: G01N21/64

CPC classification number: G01N21/6452 ,  G01N2021/6476 ,  G01N2201/0446

Abstract: A sensitive frontal approach fluorometer which is suitable for measuring the fluorescence of samples in open top microtest wells and which has an optical system for (a) directing an exciting light downwardly into the well's open top to fluorescently excite the sample and (b) detecting the sample's emitted light which passes upwardly through the well's open top.

Abstract translation: 一种灵敏的正面进场荧光计，适合于测量开顶微测井中样品的荧光，并且具有光学系统，用于（a）将激发光向下引导到井的开口顶部以荧光激发样品，以及（b）检测 样品发出的光向上通过井的开放顶部。

公开(公告)号：US4498782A

公开(公告)日：1985-02-12

申请号：US268167

申请日：1981-05-29

Applicant: David R. Proctor ,  Terry L. Foster

Inventor： David R. Proctor ,  Terry L. Foster

IPC: G01N21/25 ,  G01N21/27

CPC classification number: G01N21/253 ,  G01N21/251 ,  G01N2201/0446 ,  G01N2201/08

Abstract: The disclosure is of an assembly for determining light transmissiveness of a fluid. The assembly comprises a light source, means of holding the fluid in a light-shielded path of the light generated and a means for receiving and measuring the attenuated light passed through the fluid sample. The assembly is simple and highly portable. As such it is convenient for use with little training in places such as a physician's office, for measuring substances in immunoassay reaction mixtures.

Abstract translation: 本公开是用于确定流体的透光性的组件。 该组件包括一个光源，用于将流体保持在所产生的光的光屏蔽路径中，以及用于接收和测量通过流体样品的衰减光的装置。 组装简单，高度便携。 因此，在诸如医生办公室等用于测量免疫测定反应混合物中的物质方面的使用方便。

公开(公告)号：US4310763A

公开(公告)日：1982-01-12

申请号：US84662

申请日：1979-10-15

Applicant: John Shields

Inventor： John Shields

IPC: G01N21/05 ,  G01J1/24 ,  G01N1/10 ,  G01N1/36 ,  G01N21/01 ,  G01N21/11 ,  G01N21/25 ,  G01N21/35 ,  G01N21/49 ,  G01N33/04 ,  G01N21/26

CPC classification number: G01N21/251 ,  G01N33/04 ,  G01J1/24 ,  G01N2201/0446 ,  G01N2201/127 ,  Y10S250/91

Abstract: Electro-optical apparatus for measurement of fat, protein, lactose and water or solids in milk wherein a milk sample is pumped by a homogenizer into an optical measurement cell. The specimen in the cell is then irradiated with reference and measurement beams at differing wavelengths for fat, protein, lactose and water respectively, and signals are stored indicative of uncorrected concentrations. A scaling and correction circuit includes cross-correction circuitry for compensating the effects on each reading caused by the other constituents. The signals so corrected are then provided in percentage by weight or weight over volume on suitable digital displays.

Abstract translation: 用于测量乳中脂肪，蛋白质，乳糖和水或固体的电光装置，其中将牛奶样品通过均化器泵送到光学测量单元中。 然后分别用脂肪，蛋白质，乳糖和水分别以不同波长的参考和测量光束照射细胞中的样品，并且存储指示未校正浓度的信号。 缩放和校正电路包括用于补偿由其他成分引起的对每次读取的影响的交叉校正电路。 然后如此校正的信号以合适的数字显示器上的体积重量或重量的百分比提供。

公开(公告)号：WO2016123157A9

公开(公告)日：2016-10-20

申请号：PCT/US2016015002

申请日：2016-01-26

Applicant: CALIFORNIA INST OF TECHN

Inventor： KIM JINHO ,  YANG CHANGHUEI

IPC: G01N21/62 ,  C12M1/34

CPC classification number: G02B21/365 ,  G01N21/6452 ,  G01N21/6456 ,  G01N21/6458 ,  G01N2021/6478 ,  G01N2201/0446 ,  G02B21/0076 ,  G02B21/16 ,  H04N5/2258

Abstract: An imaging device for bright field Fourier ptychographic imaging and fluorescence imaging comprises a first fluorescence illumination source configured to provide excitation light of a first range of wavelengths to a transparent well; an optical system having a pair of objectives positioned oppositely; and an image sensor configured to receive light propagated through the optical system from the transparent well. The image sensor is configured to acquire a sequence of uniquely illuminated intensity measurements of light passing through a sample in the well based on sequential illumination at different illumination angles from a variable illumination source, and further configured to acquire a first fluorescence image of the sample based on light emitted by the sample in response to excitation light of the first range of wavelengths.

Abstract translation: 一种用于明场傅立叶谱图成像和荧光成像的成像装置，包括：第一荧光照明源，被配置为向第一透明阱提供第一波长范围的激发光; 具有相对定位的一对物镜的光学系统; 以及图像传感器，被配置为从所述透明孔接收通过所述光学系统传播的光。 图像传感器被配置为基于来自可变照明源的不同照明角度的顺序照明来获取穿过井中的样本的光的唯一照明强度测量序列，并且还被配置为获取基于样本的第一荧光图像 对由样品响应于第一波长范围的激发光而发射的光。

公开(公告)号：WO2016162874A1

公开(公告)日：2016-10-13

申请号：PCT/IL2016/050377

申请日：2016-04-08

Applicant: BACTUSENSE TECHNOLOGIES LTD.

Inventor： DU-NOUR, Ofer

IPC: G01N21/47

CPC classification number: G01N21/4788 ,  A61B5/7257 ,  G01D5/266 ,  G01N21/4738 ,  G01N2021/4726 ,  G01N2021/4797 ,  G01N2201/0446 ,  G02B21/06 ,  G02B21/362

Abstract: Systems for the monitoring of bacterial levels in samples, using spectral analysis of the light diffracted from a substrate with an ordered array of pores having diameters enabling the targets to enter them. The trapping pore array is cyclically illuminated by light of different wavelengths, and the light diffracted from the pore array is imaged by a 2-dimensional detector array, with one pixel, or a small group of pixels receiving light from each associated pore. The temporal sequence of frames provides a series of images, each from the reflection of a different wavelength. A time sequenced readout of the signal from the pixel or pixels associated with each pore region, provides a spectral plot of the reflected light from that pore region. Spectral analysis of the light intensity from this series of different wavelength enables the effective optical thickness (EOT) of each pore to be extracted.

Abstract translation: 用于监测样品中细菌水平的系统，使用从基底衍射的光的光谱分析，其具有能够使靶进入其的直径的具有直径的有序排列的孔。 捕获孔阵列由不同波长的光循环照射，并且从孔阵列衍射的光由具有一个像素的二维检测器阵列或从每个相关孔接收光的一小组像素成像。 帧的时间序列提供了一系列图像，每个图像都来自不同波长的反射。 来自与每个孔区域相关联的像素或像素的信号的时间顺序读出提供来自该孔区域的反射光的光谱图。 从该系列不同波长的光强度的光谱分析使得能够提取每个孔的有效光学厚度（EOT）。

公开(公告)号：WO2011008299A3

公开(公告)日：2011-06-23

申请号：PCT/US2010002012

申请日：2010-07-16

Applicant: GOLD STANDARD DIAGNOSTICS CORP ,  VAN PRAET PETER

Inventor： VAN PRAET PETER

IPC: G01N9/24 ,  G01N21/17 ,  G01N35/00

CPC classification number: G01N21/00 ,  G01N21/253 ,  G01N2201/0446 ,  G01N2201/0453 ,  G01N2201/0627 ,  G01N2201/10

Abstract: Light emitting diodes (LEDs) are mounted in an array to an upper structure overlying a lower structure with a plurality of light detectors thereon. Each LED is configured to overlie a separate detector. Each LED emits light at a frequency relevant for measuring optical density of a specimen. LEDs having different frequencies are included within the LED array. A corresponding array of detectors is also provided, mounted to the lower structure. Spacing between adjacent LEDs and between adjacent detectors match a spacing between wells in a microtiter plate. Spacing between the lower structure and the upper structure supporting the LEDs is sufficient for the microtiterplate to fit between. Circuitry sequentially fires individual LEDs and gathers optical density data through the detectors for specimens in the wells of the microtiter plate. The structures are then moved to a next adjacent well position on the microtiter plate and the process repeated.

Abstract translation: 发光二极管（LED）以阵列的形式安装在覆盖下部结构的上部结构上，其上具有多个光检测器。 每个LED被配置为覆盖单独的检测器。 每个LED以与测量样品的光密度相关的频率发光。 具有不同频率的LED包括在LED阵列内。 还提供了相应的检测器阵列，安装到下部结构。 相邻LED之间和相邻检测器之间的间距与微量滴定板中的孔之间的间距相匹配。 支撑LED的下部结构和上部结构之间的间隔足以使微量滴定板配合。 电路依次点火各个LED，并通过微量滴定板孔中的标本的检测器收集光密度数据。 然后将结构移动到微量滴定板上的下一个相邻的孔位置，并重复该过程。

公开(公告)号：WO2006138261A3

公开(公告)日：2007-09-07

申请号：PCT/US2006022908

申请日：2006-06-12

Applicant: STRATAGENE CALIFORNIA ,  REID TAYLOR A ,  TAYLOR ROGER H ,  ZAJAC KENNETH J

Inventor： REID TAYLOR A ,  TAYLOR ROGER H ,  ZAJAC KENNETH J

IPC: G01N21/01

CPC classification number: G01J3/10 ,  G01J3/4406 ,  G01J2003/1213 ,  G01N21/645 ,  G01N21/6452 ,  G01N21/6486 ,  G01N2021/6484 ,  G01N2201/0446 ,  G01N2201/0612 ,  G01N2201/062

Abstract: A system and method for fluorescence excitation and detection having distinct optical paths is disclosed. A system for detecting fluorescence comprises a light source(40) that emits an excitation light (48) into an illumination tube (44); a plurality of collection optics (39) located around an aperture (47) in the illumination tube for collecting fluorescence; and a detector (53) for determining the amount of fluorescence. A method for detecting fluorescence comprises emitting an excitation light (48) from a light source (40) into an illumination tube (44); directing the excitation light to an excitation filter (62); illuminating a sample with the excitation light (48) to generate an emission light; and detecting the optical characteristics of the emission light using a plurality of collection optics (39) located around the illumination tube (44).

Abstract translation: 公开了具有不同光路的用于荧光激发和检测的系统和方法。 用于检测荧光的系统包括将激发光（48）发射到照明管（44）中的光源（40）; 位于所述照明管中用于收集荧光的孔（47）周围的多个收集光学器件（39） 和用于确定荧光量的检测器（53）。 检测荧光的方法包括将光源（40）的激发光（48）发射到照明管（44）中; 将激发光引导到激发过滤器（62）; 用激发光（48）照射样品以产生发射光; 以及使用位于所述照明管（44）周围的多个收集光学器件（39）来检测所述发射光的光学特性。