公开(公告)号：US06331438B1

公开(公告)日：2001-12-18

申请号：US09448499

申请日：1999-11-24

Applicant: Jonathan W. Aylott ,  Zoe Chen-Esterlit ,  Jon H. Friedl ,  Raoul Kopelman ,  Vadim N. Savvateev ,  Joseph Shinar

Inventor： Jonathan W. Aylott ,  Zoe Chen-Esterlit ,  Jon H. Friedl ,  Raoul Kopelman ,  Vadim N. Savvateev ,  Joseph Shinar

IPC: G01N2164

CPC classification number: G01N21/66 ,  G01N21/7703 ,  G01N2201/0628

Abstract: Optical sensor, probe and array devices for detecting chemical biological, and physical analytes. The devices include an analyte-sensitive layer optically coupled to a thin film electroluminescent layer which activates the analyte-sensitive layer to provide an optical response. The optical response varies depending upon the presence of an analyte and is detected by a photodetector and analyzed to determine the properties of the analyte.

Abstract translation: 用于检测化学生物学和物理分析物的光学传感器，探针和阵列装置。 这些装置包括光学耦合到薄膜电致发光层的分析物敏感层，其激活分析物敏感层以提供光学响应。 光学响应根据分析物的存在而变化，并由光电检测器检测并分析以确定分析物的性质。

公开(公告)号：US11733168B2

公开(公告)日：2023-08-22

申请号：US17621630

申请日：2020-06-08

Applicant: SENTRONIC GMBH—GESELLSCHAFT FÜR OPTISCHE MEßSYSTEME

Inventor： Matthias Lau

IPC: G01N21/77 ,  C12M1/00 ,  C12M1/34 ,  G01N21/552 ,  A61B5/00 ,  G01N21/78

CPC classification number: G01N21/77 ,  C12M23/28 ,  C12M41/00 ,  C12M41/12 ,  C12M41/44 ,  G01N21/553 ,  A61B5/68 ,  A61B5/6803 ,  A61B5/6861 ,  G01N21/78 ,  G01N2021/7773 ,  G01N2021/7786 ,  G01N2201/0628

Abstract: The invention relates to a sensor module (1) for multiparametric analysis of a medium (105) and to the uses thereof. The sensor module (1) according to the invention is characterised by a combination of photonic and non-photonic measurement principles with parameter-sensitive coatings (103) on a substrate (100). A plurality of properties of a medium (105) can be detected over wide parameter ranges, wherein the most suitable method can be used for the corresponding parameter, at least for example with regard to the accuracy, the long-term stability, the resolution, the reproducibility, the energy consumption, the manufacturing costs, the necessary space requirements.

公开(公告)号：US09273353B2

公开(公告)日：2016-03-01

申请号：US14730989

申请日：2015-06-04

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Michael R. Gambini ,  John G. Atwood ,  Anthony L. Cerrone ,  Edward J. Lakatos ,  Eugene F. Young

IPC: C12M1/34 ,  C12M3/00 ,  C12Q1/68 ,  B01L7/00 ,  G01N21/27 ,  G01N21/64 ,  B01L3/00

CPC classification number: G01N21/6428 ,  B01L3/5085 ,  B01L7/52 ,  B01L2200/10 ,  B01L2300/0654 ,  B01L2300/0663 ,  B01L2300/1811 ,  C12Q1/686 ,  C12Q2545/101 ,  G01N21/274 ,  G01N21/645 ,  G01N21/6452 ,  G01N2021/6419 ,  G01N2021/6421 ,  G01N2021/6439 ,  G01N2021/6441 ,  G01N2021/6463 ,  G01N2021/6482 ,  G01N2201/0245 ,  G01N2201/06113 ,  G01N2201/0628 ,  G01N2201/0635 ,  G01N2201/0636 ,  G01N2201/068 ,  C12Q2563/107

Abstract: An optical instrument monitors PCR replication of DNA in a reaction apparatus having a temperature cycled block with vials of reaction ingredients including dye that fluoresces in presence of double-stranded DNA. A beam splitter passes an excitation beam to the vials to fluoresce the dye. An emission beam from the dye is passed by the beam splitter to a CCD detector from which a processor computes DNA concentration. A reference strip with a plurality of reference emitters emit reference beams of different intensity, from which the processor selects an optimum emitter for compensating for drift. Exposure time is automatically adjusted for keeping within optimum dynamic ranges of the CCD and processor. A module of the beam splitter and associated optical filters is associated with selected dye, and is replaceable for different dyes.

公开(公告)号：US20150204787A1

公开(公告)日：2015-07-23

申请号：US14404338

申请日：2013-05-30

Applicant: BOARD OF TRUSTEES OF MICHIGAN STATE UNIVERSITY

Inventor： David Kramer ,  Jeffrey Cruz ,  Christopher Hall ,  William Kent Kovac ,  Robert Zegarac

IPC: G01N21/64

CPC classification number: G01N21/6486 ,  G01N21/6408 ,  G01N21/6456 ,  G01N33/0098 ,  G01N33/025 ,  G01N2021/635 ,  G01N2021/8466 ,  G01N2201/0221 ,  G01N2201/0627 ,  G01N2201/0628

Abstract: Chlorophyll fluorescence may be studied in response to a variety of environmental cues or conditions by growing phototrophic organisms under actinic illumination. Such illumination may be punctuated or disrupted to gain information about the photosynthetic properties or performance of the phototrophic organism. Instruments or devices for carrying out the method are also described.

Abstract translation: 可以通过在光化照射下生长光养生物来响应各种环境线索或条件来研究叶绿素荧光。 这种照明可能被打断或破坏以获得关于光合生物的光合性质或性能的信息。 还描述了用于执行该方法的仪器或装置。

公开(公告)号：US08885160B2

公开(公告)日：2014-11-11

申请号：US12866078

申请日：2009-02-25

Applicant: Dominik Rabus ,  Michael Winkler ,  Christian Oberndorfer

Inventor： Dominik Rabus ,  Michael Winkler ,  Christian Oberndorfer

IPC: G01J3/00 ,  G01J3/28 ,  G01J3/02 ,  G01N21/25 ,  G01J3/04 ,  G01N21/53

CPC classification number: G01N21/255 ,  G01J3/02 ,  G01J3/021 ,  G01J3/0237 ,  G01J3/0256 ,  G01J3/0259 ,  G01J3/0291 ,  G01J3/04 ,  G01N21/532 ,  G01N2201/0628

Abstract: The compact microspectrometer for fluid media has, in a fixed spatial coordination in a housing, a light source, a fluid channel, a reflective diffraction grating, and a detector. The optical measuring path starting from the light source passes through the fluid channel and impinges on the diffraction grating. The spectral light components reflected by the diffraction grating impinge on the detector.

Abstract translation: 用于流体介质的紧凑型微光谱仪在外壳中具有固定的空间配合，光源，流体通道，反射衍射光栅和检测器。 从光源开始的光学测量路径通过流体通道并撞击在衍射光栅上。 由衍射光栅反射的光谱光分量撞击检测器。

公开(公告)号：US08809040B2

公开(公告)日：2014-08-19

申请号：US13333788

申请日：2011-12-21

Applicant: Howard G. King ,  Steven J. Boege ,  Eugene F. Young ,  Mark F. Oldham

Inventor： Howard G. King ,  Steven J. Boege ,  Eugene F. Young ,  Mark F. Oldham

IPC: C12M1/34 ,  C12M3/00 ,  G01N21/64

CPC classification number: G01N21/6428 ,  B01L7/52 ,  B01L2300/1805 ,  G01N21/6452 ,  G01N2021/6419 ,  G01N2021/6421 ,  G01N2021/6441 ,  G01N2201/062 ,  G01N2201/0627 ,  G01N2201/0628 ,  Y10S435/808

Abstract: An apparatus and method are provided for differentiating multiple detectable signals by excitation wavelength. The apparatus can include a light source that can emit respective excitation light wavelengths or wavelength ranges towards a sample in a sample retaining region, for example, in a well. The sample can contain two or more detectable markers, for example, fluorescent dyes, each of which can be capable of generating increased detectable emissions when excited in the presence of a target component. The detectable markers can have excitation wavelength ranges and/or emission wavelength ranges that overlap with the ranges of the other detectable markers. A detector can be arranged for detecting an emission wavelength or wavelength range emitted from a first marker within the overlapping wavelength range of at least one of the other markers.

Abstract translation: 提供一种用于通过激发波长区分多个可检测信号的装置和方法。 该装置可以包括可以例如在一个井中的样品保留区域中的样品发射相应的激发光波长或波长范围的光源。 样品可以包含两种或更多种可检测的标记，例如荧光染料，其中当荧光染料在靶组分存在下被激发时，其能够产生增加的可检测的发射。 可检测标记物可以具有与其它可检测标记物的范围重叠的激发波长范围和/或发射波长范围。 可以设置检测器，用于检测在至少一个其他标记的重叠波长范围内从第一标记发射的发射波长或波长范围。

公开(公告)号：US20140045187A1

公开(公告)日：2014-02-13

申请号：US14054729

申请日：2013-10-15

Applicant: APPLIED BIOSYSTEMS, LLC

Inventor： Steven J. Boege ,  Howard G. King ,  Eugene F. Young ,  Johannes P. Sluis ,  Mark F. Oldham

IPC: G01N21/64

CPC classification number: G01N21/6486 ,  B01L7/52 ,  C12Q2545/101 ,  G01N21/6452 ,  G01N2021/6419 ,  G01N2021/6421 ,  G01N2021/6439 ,  G01N2021/6441 ,  G01N2021/6463 ,  G01N2021/6482 ,  G01N2201/0245 ,  G01N2201/0628 ,  G01N2201/0635 ,  Y10S435/808 ,  Y10S435/809

Abstract: An optical instrument is provided for simultaneously illuminating two or more spaced-apart reaction regions with excitation beams generated by a light source. The light source can include an area light array of light emitting diodes, one or more solid state lasers, one or more micro-wire lasers, or a combination thereof. According to various embodiments, a Fresnel lens can be disposed along a beam bath between the light source and the reaction regions. Methods of analysis using the optical instrument are also provided.

Abstract translation: 提供了一种光学仪器，用于同时用由光源产生的激发光束照射两个或更多个间隔开的反应区域。 光源可以包括发光二极管的区域光阵列，一个或多个固态激光器，一个或多个微线激光器，或其组合。 根据各种实施例，菲涅耳透镜可以沿着光源和反应区域之间的光束槽设置。 还提供了使用光学仪器的分析方法。

公开(公告)号：US08647578B2

公开(公告)日：2014-02-11

申请号：US13503275

申请日：2010-10-20

Applicant: Patrizia Melpignano

Inventor： Patrizia Melpignano

IPC: G01N21/00

CPC classification number: G01N33/54373 ,  B01L3/502715 ,  B01L2300/027 ,  B01L2300/0636 ,  B01L2300/0654 ,  B01L2300/0825 ,  B01L2300/0851 ,  G01N21/05 ,  G01N21/6454 ,  G01N21/6486 ,  G01N2021/0346 ,  G01N2021/056 ,  G01N2021/6439 ,  G01N2201/0628

Abstract: Device to detect at least an analyte, comprising a transparent substrate (2), having a first surface (3) with which a light source (7) is associated, and a second surface (4) on which a plurality of biological protein probes (12) are disposed, a layer (6) of polymer being interposed between said second surface (4) and said biological protein probes (12). A marker (fluorophore) is associated with said analyte, having determinate characteristics of fluorescence and/or phosphorescence correlated to the emission wavelength of the light source (7). Said light source (7) is suitable to emit a light radiation in a range of wavelengths equal to 400-550 nm, inside which range the absorption peak of said marker (fluorophore) used is comprised. The value of the distance (“s”) between the wavelength corresponding to the absorption peak of the marker (fluorophore) and the wavelength corresponding to the emission peak of fluorescence (phosphorescence) is comprised between 25 and 150 nm.

Abstract translation: 用于检测至少一种分析物的装置，包括具有与光源（7）相关联的第一表面（3）的透明基底（2），以及第二表面（4），多个生物蛋白探针（ 12），聚合物层（6）插入在所述第二表面（4）和所述生物蛋白质探针（12）之间。 标记（荧光团）与所述分析物相关联，具有与光源（7）的发射波长相关的荧光和/或磷光的确定特性。 所述光源（7）适于发射在等于400-550nm的波长范围内的光辐射，其中包括所使用的所述标记（荧光团）的吸收峰。 对应于标记（荧光团）的吸收峰的波长与对应于荧光（磷光）的发射峰的波长之间的距离（“s”）的值为25-150nm。

公开(公告)号：US08557566B2

公开(公告)日：2013-10-15

申请号：US11333483

申请日：2006-01-17

Applicant: Michael R. Gambini ,  Eugene F. Young ,  Edward J. Lakatos ,  Anthony L. Cerrone ,  Susan Atwood Stone ,  Judith K. Atwood

Inventor： Michael R. Gambini ,  John G. Atwood ,  Eugene F. Young ,  Edward J. Lakatos ,  Anthony L. Cerrone

IPC: C12M1/34 ,  C12M3/00

CPC classification number: G01N21/6428 ,  B01L3/5085 ,  B01L7/52 ,  B01L2200/10 ,  B01L2300/0654 ,  B01L2300/0663 ,  B01L2300/1811 ,  C12Q1/686 ,  C12Q2545/101 ,  G01N21/274 ,  G01N21/645 ,  G01N21/6452 ,  G01N2021/6419 ,  G01N2021/6421 ,  G01N2021/6439 ,  G01N2021/6441 ,  G01N2021/6463 ,  G01N2021/6482 ,  G01N2201/0245 ,  G01N2201/06113 ,  G01N2201/0628 ,  G01N2201/0635 ,  G01N2201/0636 ,  G01N2201/068 ,  C12Q2563/107

Abstract: An optical instrument monitors PCR replication of DNA in a reaction apparatus having a temperature cycled block with vials of reaction ingredients including dye that fluoresces in presence of double-stranded DNA. A beam splitter passes an excitation beam to the vials to fluoresce the dye. An emission beam from the dye is passed by the beam splitter to a CCD detector from which a processor computes DNA concentration. A reference strip with a plurality of reference emitters emit reference beams of different intensity, from which the processor selects an optimum emitter for compensating for drift. Exposure time is automatically adjusted for keeping within optimum dynamic ranges of the CCD and processor. A module of the beam splitter and associated optical filters is associated with selected dye, and is replaceable for different dyes.

公开(公告)号：US20120229891A1

公开(公告)日：2012-09-13

申请号：US13418097

申请日：2012-03-12

Applicant: Zhaowei Liu ,  Feifei Wei

Inventor： Zhaowei Liu ,  Feifei Wei

IPC: G02B13/14

CPC classification number: G02B5/008 ,  G01N21/553 ,  G01N21/6458 ,  G01N21/648 ,  G01N2201/062 ,  G01N2201/0628

Abstract: Plasmonic condensers for generating surface plasmon at an evanescent wave surface can include a substrate layer, a metal layer comprising the evanescent wave surface; and a media layer disposed between the metal layer and the substrate layer. The media layer can be active or passive and can include a source of radiation that interacts with the metal layer to create surface plasmons that are not substantially optically detectable as far field radiation until an interfering object is brought into proximity with the evanescent wave surface. When an interfering object such as a sample or specimen is brought into proximity with the evanescent wave surface, it causes coupling of at least some of the surface plasmons into propagating radiation detectable by an objective lens. Systems, methods, and the like are disclosed, as are features of a plasmonic meta-materials illuminator.

Abstract translation: 用于在ev逝波表面产生表面等离子体激元的等离子体冷凝器可以包括基底层，包含ev逝波面的金属层; 以及设置在金属层和基底层之间的介质层。 介质层可以是有源的或无源的，并且可以包括与金属层相互作用的辐射源，以产生表面等离子体，这些表面等离激元不是远场光辐射，直到干扰物体接近于ev逝波面。 当诸如样品或样品的干扰物体与ev逝波表面接近时，其使至少一些表面等离子体激元耦合到由物镜可检测的传播辐射。 公开了系统，方法等，以及等离子体激元间材料照明器的特征。