公开(公告)号：WO2012071275A2

公开(公告)日：2012-05-31

申请号：PCT/US2011/061425

申请日：2011-11-18

Applicant: BIO-RAD LABORATORIES, INC. ,  ARCHER, Robert M. ,  HSIUNG, Frank ,  PATT, Paul

Inventor： ARCHER, Robert M. ,  HSIUNG, Frank ,  PATT, Paul

IPC: C12Q1/00

CPC classification number: G01N33/5094 ,  G01N15/0606 ,  G01N15/1463 ,  G01N2015/0288 ,  G01N2015/149

Abstract: Adherent cells bearing characteristics that are detectable only in the adherent state can be sorted on the basis of these characteristics independently of their adherent state, by applying a transformable label to the entire population of cells, both those bearing the characteristics of interest and those not, in their adherent state and identifying the locations of the cells of interest on the adherent surface. The cells of interest, or all cells other than those of interest, are then selectively treated to transform the labels and achieve differentiation between the cells of interest and the remaining cells. All cells are then released from the adherent state and sorted in the same manner as non-adherent cells but on the basis of whether the labels are transformed or not transformed.

Abstract translation: 具有仅在粘附状态下可检测的特征的粘附细胞可以根据这些特征独立于其粘附状态进行分类，通过将可变形标记应用于整个细胞群体，具有感兴趣特征的那些， 在其粘附状态下并且识别感兴趣的细胞在贴壁表面上的位置。 然后选择性地处理感兴趣的细胞或所有不同于感兴趣的细胞的细胞，以转化标记并实现感兴趣的细胞和剩余的细胞之间的分化。 然后将所有细胞从粘附状态释放并以与非贴壁细胞相同的方式进行分选，但是基于标记是否被转化或未转化。

公开(公告)号：WO2012071275A3

公开(公告)日：2012-07-26

申请号：PCT/US2011061425

申请日：2011-11-18

Applicant: BIO RAD LABORATORIES ,  ARCHER ROBERT M ,  HSIUNG FRANK ,  PATT PAUL

Inventor： ARCHER ROBERT M ,  HSIUNG FRANK ,  PATT PAUL

IPC: C12N5/00 ,  C12N5/02 ,  C12N5/071 ,  G01J1/56 ,  G01J3/30 ,  G01N23/00

CPC classification number: G01N33/5094 ,  G01N15/0606 ,  G01N15/1463 ,  G01N2015/0288 ,  G01N2015/149

Abstract: Adherent cells bearing characteristics that are detectable only in the adherent state can be sorted on the basis of these characteristics independently of their adherent state, by applying a transformable label to the entire population of cells, both those bearing the characteristics of interest and those not, in their adherent state and identifying the locations of the cells of interest on the adherent surface. The cells of interest, or all cells other than those of interest, are then selectively treated to transform the labels and achieve differentiation between the cells of interest and the remaining cells. All cells are then released from the adherent state and sorted in the same manner as non-adherent cells but on the basis of whether the labels are transformed or not transformed.

Abstract translation: 通过对整个细胞群施加可转化的标记（无论是具有感兴趣特征还是未感兴趣特征的细胞），可以根据这些特征独立于它们的粘附状态对具有仅可检测到粘附状态的特征的粘附细胞进行分类， 处于其粘附状态并识别粘附表面上感兴趣细胞的位置。 然后选择性处理感兴趣的细胞或感兴趣的细胞以外的所有细胞以转化标记并实现感兴趣的细胞和剩余细胞之间的区分。 然后从贴壁状态释放所有细胞，并按照与非贴壁细胞相同的方式分类，但基于标记是否被转化或未转化。