公开(公告)号：WO2007064191A1

公开(公告)日：2007-06-07

申请号：PCT/NL2005/000828

申请日：2005-12-02

Applicant: STICHTING KATHOLIEKE UNIVERSITEIT ,  DAAMEN, Wilhelmina, Francisca ,  GEUTJES, Petrus, Johannes ,  VAN KUPPEVELT, Antonius, Henricus, Minardus, Severus, Maria

Inventor： DAAMEN, Wilhelmina, Francisca ,  GEUTJES, Petrus, Johannes ,  VAN KUPPEVELT, Antonius, Henricus, Minardus, Severus, Maria

IPC: A61K9/16

CPC classification number: A61K9/1652 ,  A61K9/1658 ,  Y10T428/2984

Abstract: Described is a method for obtaining hollow particles, having a particle wall and a particle lumen, the particle having dimensions of between 1 nm and 100 µm, from a mixture comprising a liquid medium comprising at least one colloid or solute, the method comprising freezing said mixture and lyophilising the obtained frozen mixture, characterised in that a volume of at least 0.1 µl of the mixture is subjected to a freezing step comprising: (a) (1) quench freezing the mixture resulting in a quench frozen mixture, and (2) incubating said quench frozen mixture at a temperature above the quench freezing temperature and below the melting point of the liquid medium, or b) (1) reducing the temperature of the mixture at a rate of 1 to 100 °C/minute to below the freezing temperature of the mixture and (2) incubating said frozen mixture at a temperature above the temperature of the mixture and below the melting point of the liquid medium. Further, hollow particles obtainable by the said method, compositions comprising said hollow particles and uses thereof are described.

Abstract translation: 描述了用于从包含液体介质的混合物获得具有颗粒壁和颗粒腔的中空颗粒的方法，所述颗粒的尺寸在1nm和100μm之间，所述液体介质包含至少 一种胶体或溶质，所述方法包括冷冻所述混合物并冻干所获得的冷冻混合物，其特征在于将至少0.1μl体积的混合物进行冷冻步骤，所述冷冻步骤包括：（a）（1） （2）将所述淬火冷冻混合物在高于骤冷冻温度且低于液体介质熔点的温度下孵育，或b）（1）以一定速率降低混合物的温度 1至100℃/分钟至低于混合物的冷冻温度，和（2）在高于混合物温度且低于液体介质熔点的温度下温育所述冷冻混合物。 此外，描述了通过所述方法获得的中空颗粒，包含所述中空颗粒的组合物及其用途。

公开(公告)号：WO2007064191A8

公开(公告)日：2007-09-27

申请号：PCT/NL2005000828

申请日：2005-12-02

Applicant: STICHTING KATHOLIEKE UNIV ,  DAAMEN WILHELMINA FRANCISCA ,  GEUTJES PETRUS JOHANNES ,  VAN KUPPEVELT ANTONIUS HENRICU

Inventor： DAAMEN WILHELMINA FRANCISCA ,  GEUTJES PETRUS JOHANNES ,  VAN KUPPEVELT ANTONIUS HENRICU

IPC: A61K9/16

CPC classification number: A61K9/1652 ,  A61K9/1658 ,  Y10T428/2984

Abstract: Described is a method for obtaining hollow particles, having a particle wall and a particle lumen, the particle having dimensions of between 1 nm and 100 µm, from a mixture comprising a liquid medium comprising at least one colloid or solute, the method comprising freezing said mixture and lyophilising the obtained frozen mixture, characterised in that a volume of at least 0.1 µl of the mixture is subjected to a freezing step comprising: (a) (1) quench freezing the mixture resulting in a quench frozen mixture, and (2) incubating said quench frozen mixture at a temperature above the quench freezing temperature and below the melting point of the liquid medium, or b) (1) reducing the temperature of the mixture at a rate of 1 to 100 °C/minute to below the freezing temperature of the mixture and (2) incubating said frozen mixture at a temperature above the temperature of the mixture and below the melting point of the liquid medium. Further, hollow particles obtainable by the said method, compositions comprising said hollow particles and uses thereof are described.

Abstract translation: 描述了一种从包含至少一种胶体或溶质的液体介质的混合物中获得具有颗粒壁和颗粒内腔，尺寸为1nm至100μm的颗粒的中空颗粒的方法，所述方法包括将所述 混合物并冻干所得的冷冻混合物，其特征在于至少0.1μl混合物的体积进行冷冻步骤，包括：（a）（1）骤冷冷冻混合物，得到骤冷的混合物，和（2） 在高于猝灭冷冻温度并低于液体培养基的熔点的温度下孵育所述骤冷冷冻混合物，或b）（1）以1至100℃/分钟的速率将混合物的温度降低至低于冰点 混合物的温度和（2）在高于混合物温度并低于液体介质的熔点的温度下孵育所述冷冻混合物。 此外，描述了可通过所述方法获得的中空颗粒，包含所述中空颗粒的组合物及其用途。