公开(公告)号：US20060247154A1

公开(公告)日：2006-11-02

申请号：US11363373

申请日：2006-02-24

Applicant: Stephen Palmieri ,  Dylan Bulseco

Inventor： Stephen Palmieri ,  Dylan Bulseco

IPC: A61K38/16 ,  C07K14/42

CPC classification number: C07K14/42 ,  G01N33/542

Abstract: A novel method for purifying various lectins is disclosed. More specifically a novel method for purifying Concanavalin A is set forth. Methods of expressing purifying and characterizing a mutant Concanavalin A, and sensors including the foregoing are also disclosed.

Abstract translation: 公开了一种用于纯化各种凝集素的新方法。 更具体地提出了一种用于纯化伴刀豆球蛋白A的新方法。 还公开了表达纯化和表征突变型伴刀豆球蛋白A的方法，以及包括前述物质的传感器。

公开(公告)号：US20070291254A1

公开(公告)日：2007-12-20

申请号：US11834168

申请日：2007-08-06

Applicant: David Wolf ,  Dylan Bulseco

Inventor： David Wolf ,  Dylan Bulseco

IPC: G01N21/00

CPC classification number: G01N21/6428 ,  G01J3/4406 ,  G01J3/457 ,  G01N21/645 ,  G01N33/582 ,  G01N2021/6417 ,  G01N2021/6482 ,  G01N2021/6484

Abstract: Disclosed is a portable fluorescence correlation spectroscopy instrument that includes an excitation source, at least one of a light focusing element positioned to receive light emitted by the excitation source, a detector for detecting light, the detector positioned to receive light emitted by a sample excited by the excitation source, and a correlator coupled to the detector, the correlator for processing data received at the detector and providing data including autocorrelation data, crosscorrelation data, or a combination thereof.

Abstract translation: 公开了一种便携式荧光相关光谱仪器，其包括激发源，定位成接收由激发源发射的光的光聚焦元件中的至少一个，用于检测光的检测器，被定位成接收由由 激励源和耦合到检测器的相关器，相关器，用于处理在检测器处接收的数据，并提供包括自相关数据，互相关数据或其组合的数据。

公开(公告)号：US20070207498A1

公开(公告)日：2007-09-06

申请号：US11513003

申请日：2006-08-30

Applicant: Stephen Palmieri ,  Dylan Bulseco ,  David Matzinger

Inventor： Stephen Palmieri ,  Dylan Bulseco ,  David Matzinger

IPC: G01N33/53 ,  C07K14/42

CPC classification number: G01N33/542 ,  C07K14/42

Abstract: Embodiments of the invention provide for compositions comprising purified polypeptides such as purified Concanavalin A (ConA) mutants. In addition, embodiments provide for polypeptides and nucleic acids encoding those polypeptides, such as mutant ConA with reduced dimer-dimer interactions compared to wild type ConA. Some embodiments also provide for sensors comprising the polypeptides disclosed herein. The embodiments also provide an improved method of producing recombinant mutant ConA.

Abstract translation: 本发明的实施方案提供包含纯化多肽的组合物，例如纯化的伴刀豆球蛋白A（ConA）突变体。 此外，实施方案提供编码那些多肽的多肽和核酸，例如与野生型ConA相比，具有降低的二聚体 - 二聚体相互作用的突变型ConA。 一些实施方案还提供包含本文公开的多肽的传感器。 实施方案还提供了重组突变型ConA的改良方法。