公开(公告)号：JP2008295301A

公开(公告)日：2008-12-11

申请号：JP2005265164

申请日：2005-09-13

Applicant: Fuaruko Bio Syst:Kk ,  Hideki Ishikawa ,  Matsuura Nariaki ,  松浦 成昭 ,  株式会社ファルコバイオシステムズ ,  秀樹 石川

Inventor： ISHIKAWA HIDEKI

IPC: C12Q1/68

CPC classification number: C12Q1/6886 ,  C12Q2600/158 ,  G01N33/57484 ,  G01N2333/4703

Abstract: PROBLEM TO BE SOLVED: To provide a means capable of adequately analyzing a tumorigenic risk utilizing a biological index. SOLUTION: A method for analyzing the tumorigenic risk includes the steps which include (a) a step of measuring expression levels of two or more indicator genes selected from a cell growth-promoting gene cluster, a cell growth-inhibiting gene cluster, an apoptosis-promoting gene cluster, an apoptosis-inhibiting gene cluster, a cell differentiation-promoting gene cluster and a cell differentiation-inhibiting gene cluster in a test tissue collected from a non-tumorous part of a specimen; (b) a step of measuring the expression level of a housekeeping gene in the same tissue; (c) a step of correcting the measured values for the expression level of each of the indicator genes, obtained in step (a) with the measured values for the expression level of the housekeeping gene obtained in step (b) and employing the corrected values as a tumorigenic index related to each of the indicator genes; (d) a step of comparing the tumorigenic index of each of the indicator genes, obtained in step (c) with the preset standard value and evaluating the tumorigenic index; and (e) a step of combining two or more evaluations for each of the indicator genes obtained in step (d). COPYRIGHT: (C)2009,JPO&INPIT

Abstract translation: 要解决的问题：提供能够利用生物学指标充分分析致瘤风险的手段。 解决方案：用于分析致瘤风险的方法包括以下步骤：（a）测量选自细胞生长促进基因簇，细胞生长抑制基因簇的两个或更多个指示基因的表达水平的步骤， 在从样本的非肿瘤部分收集的测试组织中的凋亡促进基因簇，细胞凋亡抑制基因簇，细胞分化促进基因簇和细胞分化抑制基因簇; （b）测量同一组织中持家基因的表达水平的步骤; （c）使用步骤（b）中获得的管家基因的表达水平的测量值，将步骤（a）中获得的每个指示基因的表达水平的测定值校正，并采用校正值 作为与每个指标基因相关的致瘤指数; （d）将步骤（c）中获得的每个指示基因的致瘤指数与预设标准值进行比较并评价致瘤指数的步骤; 和（e）对步骤（d）中获得的每个指示基因组合两个或多个评估的步骤。 版权所有（C）2009，JPO＆INPIT

公开(公告)号：JP2004344108A

公开(公告)日：2004-12-09

申请号：JP2003146772

申请日：2003-05-23

Applicant: Arkray Inc ,  Fuaruko Bio Syst:Kk ,  アークレイ株式会社 ,  株式会社ファルコバイオシステムズ

Inventor： KAMATA TATSUO ,  IZUMISAWA YUJI ,  UEDA KOZO ,  MORISAWA TAE

IPC: G01N33/48 ,  C12N15/09 ,  C12Q1/04 ,  C12Q1/68 ,  G01N33/50

CPC classification number: Y02A50/451

Abstract: PROBLEM TO BE SOLVED: To simply realize a range of currents such as pretreatment, bacteriolysis and amplification without using a centrifugal separator after re-dispersing precipitate of acid-fast bacteria after NALC-NaOH treatment and a specific reagent in a short time in a pretreatment for lysing and extracting genes in the acid-fast bacteria.
SOLUTION: The method for pre-treating the acid-fast bacteria comprises carrying out NALC-NaOH treatment of a specimen in which the content of the acid-fast bacterium is suspected and directly passing the treated material of the specimen through a lysing means without carrying out centrifugal separation treatment. The treated material of the specimen may be passed through the lysing means after dispersing the treated material of the specimen by only sterilized distilled water.
COPYRIGHT: (C)2005,JPO&NCIPI

公开(公告)号：JP2012135290A

公开(公告)日：2012-07-19

申请号：JP2010291409

申请日：2010-12-28

Applicant: Fuaruko Bio Syst:Kk ,  株式会社ファルコバイオシステムズ

Inventor： FURUI YOSUKE ,  MARUSE HIDEAKI ,  FUKUI TAKASHI ,  HANAZAWA KISABURO

IPC: C12Q1/68 ,  C12N15/09

Abstract: PROBLEM TO BE SOLVED: To provide a method of detecting K-ras gene mutations, wherein blood can be used as inspection material in place of cancer tissue.SOLUTION: The method of detecting K-ras gene mutations includes a process for extracting circulating DNA from a blood sample that is selected from the group consisting of a subject's blood, plasma, and serum and a process for reacting the extracted circulating DNA with a PNA probe containing a forward primer, a reverse primer, and the codon 12 and the codon 13 of K-ras to perform a PCR reaction to detect whether the codon 12 and the codon 13 of K-ras have mutated.

Abstract translation: 待解决的问题：提供检测K-ras基因突变的方法，其中可以使用血液作为检查材料代替癌组织。 解决方案：检测K-ras基因突变的方法包括从选自受试者的血液，血浆和血清的血液样品中提取循环DNA的方法和使提取的循环DNA反应的方法 使用含有正向引物，反向引物以及K-ras的密码子12和密码子13的PNA探针进行PCR反应以检测K-ras的密码子12和密码子13是否已突变。 版权所有（C）2012，JPO＆INPIT

公开(公告)号：JP2005027567A

公开(公告)日：2005-02-03

申请号：JP2003271153

申请日：2003-07-04

Applicant: Fuaruko Bio Syst:Kk ,  株式会社ファルコバイオシステムズ

Inventor： HATTORI HIROYOSHI ,  ODA SHINYA ,  MAEHARA YOSHIHIKO

IPC: C12N15/09 ,  C12Q1/68

Abstract: PROBLEM TO BE SOLVED: To provide a quick and simple method for quantifying mixed chimera in transplanting hematopoietic stem cells in high sensitivity and quantifying capability.
SOLUTION: The method for quantifying the mixed chimera comprises making a quantitative analysis of DNA in the mixture of respective DNA amplified products for a plurality of specimens using one or more sets of primer sequences for amplifying a microsatellite region and a plurality of fluorescent marks.
COPYRIGHT: (C)2005,JPO&NCIPI

Abstract translation: 要解决的问题：提供一种快速简单的定量混合嵌合体的方法，以高灵敏度和量化能力移植造血干细胞。 解决方案：定量混合嵌合体的方法包括使用一组或多组用于扩增微卫星区的引物序列和多个荧光的多个样品对各DNA扩增产物的混合物中的DNA进行定量分析 分数。 版权所有（C）2005，JPO＆NCIPI

公开(公告)号：JP2005082558A

公开(公告)日：2005-03-31

申请号：JP2003318752

申请日：2003-09-10

Applicant: Fuaruko Bio Syst:Kk ,  株式会社ファルコバイオシステムズ

Inventor： SATO MAKOTO ,  OZAKI SHUNJI ,  KOMOTO MASAIKU ,  SETO SHOSUKE

IPC: G01N33/569 ,  C07K16/08 ,  C12N5/10 ,  C12P21/08 ,  G01N33/577

Abstract: PROBLEM TO BE SOLVED: To provide a monoclonal or polyclonal antibody against a synthetic peptide, and a high sensitivity detection method of SRSV (small round structured virus, norovirus) infection by using this antibody. SOLUTION: The monoclonal or polyclonal antibody against a synthetic peptide comprises a sequence: Cys Asp Thr Asn Asn Asp Phe Gln Thr Gly Gln Asn Thr Lys Phe. A detection reagent, the detection method, and a detection kit for an antigen or an antigenetic fragment of SRSV comprise this antibody, and a hybridoma for producing the monoclonal antibody is disclosed. COPYRIGHT: (C)2005,JPO&NCIPI

Abstract translation: 要解决的问题：提供针对合成肽的单克隆抗体或多克隆抗体，以及通过使用该抗体的SRSV（小圆结构病毒，诺如病毒）感染的高灵敏度检测方法。 解决方案：针对合成肽的单克隆或多克隆抗体包含序列：Cys Asp Thr Asn Asn Asp Phe Gln Thr Gly Gln Asn Thr Lys Phe。 SRSV的抗原或抗原性片段的检测试剂，检测方法和检测试剂盒包含该抗体，公开了用于制备单克隆抗体的杂交瘤。 版权所有（C）2005，JPO＆NCIPI