公开(公告)号：WO2010144683A3

公开(公告)日：2011-02-03

申请号：PCT/US2010038141

申请日：2010-06-10

Applicant: MICRONICS INC ,  BATTRELL C FREDERICK ,  HOEKSTRA DENISE MAXINE ,  HAAB JOAN ,  WILLIFORD JOHN R ,  GATES SABRINA N ,  LEBER ELIZABETH R ,  SPRAGUE ISAAC

Inventor： BATTRELL C FREDERICK ,  HOEKSTRA DENISE MAXINE ,  HAAB JOAN ,  WILLIFORD JOHN R ,  GATES SABRINA N ,  LEBER ELIZABETH R ,  SPRAGUE ISAAC

IPC: C12Q1/68

CPC classification number: C12Q1/686 ,  C12Q1/6806 ,  C12Q2527/125

Abstract: Manufacturing methods and compositions are described for production of self-contained microfluidic cartridge devices with on-board reagents for molecular biological testing. Sensitive reagents are stored in dry form without lyophilization or freezing, and reconstituted at the point of use with either a biological sample or a sample eluate at the point of use. Manufacturing methods include sheet and roll fabrication processes where the reagents are printed in place and sealed within individual microfluidic cartridges before gel vitrification.

Abstract translation: 描述制造方法和组合物用于生产具有用于分子生物学测试的载体试剂的自含微流控盒装置。 敏感试剂以干燥形式储存而不进行冻干或冷冻，并在使用时与生物样品或样品洗脱液在使用时重构。 制造方法包括片和卷制造方法，其中将试剂印刷在适当位置并在凝胶玻璃化之前在单个微流体盒内密封。

公开(公告)号：WO2010144682A1

公开(公告)日：2010-12-16

申请号：PCT/US2010/038140

申请日：2010-06-10

Applicant: MICRONICS, INC. ,  BATTRELL, C., Frederick ,  HOEKSTRA, Denise Maxine ,  HAAB, Joan ,  WILLIFORD, John R.

Inventor： BATTRELL, C., Frederick ,  HOEKSTRA, Denise Maxine ,  HAAB, Joan ,  WILLIFORD, John R.

IPC: C12N9/96 ,  C12N9/12 ,  C12N11/00 ,  C12Q1/68 ,  B01L3/00

CPC classification number: C12N9/96 ,  B01L3/5027 ,  B01L2200/16 ,  C12N9/1252

Abstract: Formulations for dry storage of PCR reagents are described. These formulations find use in manufacture of self-contained microfluidic card devices for PCR clinical testing in which the reagents are reconstituted at the point of testing. In these cards, TAQ polymerase is stored "on-board" in vitrified dry form without lyophilization or freezing, and is reconstituted by either the sample or a sample eluate during the assay.

Abstract translation: 描述了PCR试剂的干燥储存制剂。 这些配方可用于制造用于PCR临床测试的自给自足的微流控卡装置，其中试剂在测试点重构。 在这些卡中，TAQ聚合酶以玻璃化干燥形式储存在板上而不进行冻干或冷冻，并且在测定期间由样品或样品洗脱液重构。

公开(公告)号：WO2008147382A1

公开(公告)日：2008-12-04

申请号：PCT/US2007020810

申请日：2007-09-27

Applicant: MICRONICS INC ,  BATTRELL C FREDERICK ,  GERDES JOHN ,  BREIDFORD WAYNE L ,  CAPODANNO JASON ,  MORDUE STEPHEN ,  CLEMMENS JOHN ,  HOEKSTRA DENISE MAXINE ,  LANCASTER CHRISTY A ,  WILLIFORD JOHN R ,  MALONEY PATRICK ,  HAAB JOAN

Inventor： BATTRELL C FREDERICK ,  GERDES JOHN ,  BREIDFORD WAYNE L ,  CAPODANNO JASON ,  MORDUE STEPHEN ,  CLEMMENS JOHN ,  HOEKSTRA DENISE MAXINE ,  LANCASTER CHRISTY A ,  WILLIFORD JOHN R ,  MALONEY PATRICK ,  HAAB JOAN

IPC: B01L3/00 ,  B01F13/00 ,  G01N33/00

CPC classification number: G01N33/5302 ,  B01F11/0071 ,  B01F13/0059 ,  B01L3/502715 ,  B01L7/52 ,  B01L2300/0636 ,  B01L2300/069 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2400/0481 ,  B01L2400/0487 ,  B01L2400/0638

Abstract: Combinations of microfluidic diagnostic testing modules (71, 72) for simultaneous evaluations of serological and molecular biological targets are provided, and include panel testing for both antibodies (or antigens) and nucleic acid targets in one single-use device. These improvements are directed to evaluating the overall progress and activity of a pathogenic process in real time, at the point of care, not merely the presence or absence of a particular diagnostic marker, which can often be incomplete or misleading.

Abstract translation: 提供了用于同时评估血清学和分子生物学靶标的微流体诊断测试模块（71,72）的组合，并且包括在一次性使用的装置中对抗体（或抗原）和核酸靶标的面板测试。 这些改进旨在评估致病过程的总体进展和活动，而不仅仅是特定诊断标记的存在或不存在，这通常是不完整的或具有误导性的。

公开(公告)号：WO2008147382A8

公开(公告)日：2008-12-04

申请号：PCT/US2007/020810

申请日：2007-09-27

Applicant: MICRONICS, INC. ,  BATTRELL, C., Frederick ,  GERDES, John ,  BREIDFORD, Wayne, L. ,  CAPODANNO, Jason ,  MORDUE, Stephen ,  CLEMMENS, John ,  HOEKSTRA, Denise Maxine ,  LANCASTER, Christy, A. ,  WILLIFORD, John, R. ,  MALONEY, Patrick ,  HAAB, Joan

Inventor： BATTRELL, C., Frederick ,  GERDES, John ,  BREIDFORD, Wayne, L. ,  CAPODANNO, Jason ,  MORDUE, Stephen ,  CLEMMENS, John ,  HOEKSTRA, Denise Maxine ,  LANCASTER, Christy, A. ,  WILLIFORD, John, R. ,  MALONEY, Patrick ,  HAAB, Joan

IPC: B01L3/00 ,  G01N33/00 ,  B01F13/00

Abstract: Combinations of microfluidic diagnostic testing modules (71, 72) for simultaneous evaluations of serological and molecular biological targets are provided, and include panel testing for both antibodies (or antigens) and nucleic acid targets in one single-use device. These improvements are directed to evaluating the overall progress and activity of a pathogenic process in real time, at the point of care, not merely the presence or absence of a particular diagnostic marker, which can often be incomplete or misleading.

公开(公告)号：WO2010120786A1

公开(公告)日：2010-10-21

申请号：PCT/US2010/030901

申请日：2010-04-13

Applicant: MICRONICS, INC. ,  SPRAGUE, Isaac ,  EMSWILER, John, E. ,  BATTRELL, C., Frederick ,  HAAB, Joan ,  PENNELL, Sean, M. ,  KAY, Justin, L. ,  MILLER, Zane, B. ,  DAIBER, Troy, D.

Inventor： SPRAGUE, Isaac ,  EMSWILER, John, E. ,  BATTRELL, C., Frederick ,  HAAB, Joan ,  PENNELL, Sean, M. ,  KAY, Justin, L. ,  MILLER, Zane, B. ,  DAIBER, Troy, D.

IPC: B01L3/00 ,  G01N33/52

CPC classification number: B01L3/502 ,  B01L3/5025 ,  B01L2300/0864 ,  B01L2400/049 ,  G01N33/52 ,  Y10T436/25

Abstract: A microfluidic cartridge including on-board dry reagents and microfluidic circuitry for determining a clinical analyte or analytes from a few microliters of liquid sample; with docking interface for use in a host workstation, the workstation including a pneumatic fluid controller and spectrophotometer for monitoring analytical reactions in the cartridge.

Abstract translation: 一种微流控柱，包括板上干试剂和微流体电路，用于确定临床分析物或几微升液体样品的分析物; 具有用于主机工作站的对接界面，工作站包括气动流体控制器和分光光度计，用于监测盒中的分析反应。

公开(公告)号：WO2009018473A1

公开(公告)日：2009-02-05

申请号：PCT/US2008/071810

申请日：2008-07-31

Applicant: MICRONICS, INC. ,  BATTRELL, C., Frederick ,  CAPODANNO, Jason ,  CLEMMENS, John ,  HAAB, Joan ,  GERDES, John

Inventor： BATTRELL, C., Frederick ,  CAPODANNO, Jason ,  CLEMMENS, John ,  HAAB, Joan ,  GERDES, John

IPC: G01N1/02

CPC classification number: G01N1/02 ,  A61B10/0045 ,  A61B10/0096 ,  B01L3/502715 ,  B01L3/5029 ,  B01L7/52 ,  B01L2200/027 ,  B01L2200/082 ,  B01L2200/10 ,  B01L2300/069 ,  B01L2300/0854 ,  B01L2300/087 ,  B01L2400/0415 ,  B01L2400/0487 ,  G01N2001/028

Abstract: Biohazard specimen collection containers are provided with an external disposable skin, that is stripped away and discarded after the biohazardous specimen is collected, thus reducing or eliminating objectionable or dangerous residues on the outside surfaces of the container. Further, we teach that the sample collection container with external disposable skin may also serve as an integrated microfluidic biosample processing and analytical device, thereby providing a single entry, disposable assay unit, kit and system for "world-to-result" clinical diagnostic testing. These integrated assay devices are provided with synergic, multiple safe-handling features for protecting healthcare workers who handle them. The modified collection containers and analytical devices find application, for example, in PCR detection of infectious organisms or pathogenic markers collected on a swab.

Abstract translation: 生物危害标本收集容器设置有外部一次性皮肤，其在收集生物危险样本之后被剥离并丢弃，从而减少或消除容器外表面上的令人反感或危险的残留物。 此外，我们教导，具有外部一次性皮肤的样品收集容器也可用作集成的微流体生物样品处理和分析装置，从而为“世界到结果”临床诊断测试提供单个入口，一次性测定单元，试剂盒和系统 。 这些集成测定装置具有协同的多重安全处理功能，用于保护处理它们的医护人员。 经修改的收集容器和分析装置可以应用于例如感​​染生物的PCR检测或在拭子上收集的病原性标记物。

公开(公告)号：WO2010144683A2

公开(公告)日：2010-12-16

申请号：PCT/US2010/038141

申请日：2010-06-10

Applicant: MICRONICS, INC. ,  BATTRELL, C., Frederick ,  HOEKSTRA, Denise Maxine ,  HAAB, Joan ,  WILLIFORD, John R. ,  GATES, Sabrina N. ,  LEBER, Elizabeth R. ,  SPRAGUE, Isaac

Inventor： BATTRELL, C., Frederick ,  HOEKSTRA, Denise Maxine ,  HAAB, Joan ,  WILLIFORD, John R. ,  GATES, Sabrina N. ,  LEBER, Elizabeth R. ,  SPRAGUE, Isaac

IPC: C12Q1/68

CPC classification number: C12Q1/686 ,  C12Q1/6806 ,  C12Q2527/125

Abstract: Manufacturing methods and compositions are described for production of self-contained microfluidic cartridge devices with on-board reagents for molecular biological testing. Sensitive reagents are stored in dry form without lyophilization or freezing, and reconstituted at the point of use with either a biological sample or a sample eluate at the point of use. Manufacturing methods include sheet and roll fabrication processes where the reagents are printed in place and sealed within individual microfluidic cartridges before gel vitrification.

Abstract translation: 描述制造方法和组合物用于生产具有用于分子生物学测试的载体试剂的自含微流控盒装置。 敏感试剂以干燥形式储存而不进行冻干或冷冻，并在使用时与生物样品或样品洗脱液在使用时重构。 制造方法包括片和卷制造方法，其中将试剂印刷在适当位置并在凝胶玻璃化之前在单个微流体盒内密封。