公开(公告)号：WO2010144683A3

公开(公告)日：2011-02-03

申请号：PCT/US2010038141

申请日：2010-06-10

Applicant: MICRONICS INC ,  BATTRELL C FREDERICK ,  HOEKSTRA DENISE MAXINE ,  HAAB JOAN ,  WILLIFORD JOHN R ,  GATES SABRINA N ,  LEBER ELIZABETH R ,  SPRAGUE ISAAC

Inventor： BATTRELL C FREDERICK ,  HOEKSTRA DENISE MAXINE ,  HAAB JOAN ,  WILLIFORD JOHN R ,  GATES SABRINA N ,  LEBER ELIZABETH R ,  SPRAGUE ISAAC

IPC: C12Q1/68

CPC classification number: C12Q1/686 ,  C12Q1/6806 ,  C12Q2527/125

Abstract: Manufacturing methods and compositions are described for production of self-contained microfluidic cartridge devices with on-board reagents for molecular biological testing. Sensitive reagents are stored in dry form without lyophilization or freezing, and reconstituted at the point of use with either a biological sample or a sample eluate at the point of use. Manufacturing methods include sheet and roll fabrication processes where the reagents are printed in place and sealed within individual microfluidic cartridges before gel vitrification.

Abstract translation: 描述制造方法和组合物用于生产具有用于分子生物学测试的载体试剂的自含微流控盒装置。 敏感试剂以干燥形式储存而不进行冻干或冷冻，并在使用时与生物样品或样品洗脱液在使用时重构。 制造方法包括片和卷制造方法，其中将试剂印刷在适当位置并在凝胶玻璃化之前在单个微流体盒内密封。

公开(公告)号：WO2010040103A1

公开(公告)日：2010-04-08

申请号：PCT/US2009/059444

申请日：2009-10-02

Applicant: MICRONICS, INC. ,  BATTRELL, C., Frederick ,  WIERZBICKI, Diane ,  CLEMMENS, John ,  CAPODANNO, Jason ,  WILLIFORD, John, R. ,  ELMUFDI, Carolina ,  SPRAGUE, Isaac

Inventor： BATTRELL, C., Frederick ,  WIERZBICKI, Diane ,  CLEMMENS, John ,  CAPODANNO, Jason ,  WILLIFORD, John, R. ,  ELMUFDI, Carolina ,  SPRAGUE, Isaac

IPC: B01L3/00 ,  G01N33/80

CPC classification number: G01N33/53 ,  B01L3/502715 ,  B01L3/50273 ,  B01L3/502738 ,  B01L3/502746 ,  B01L3/502761 ,  B01L3/502776 ,  B01L2200/0636 ,  B01L2300/0627 ,  B01L2300/0816 ,  B01L2300/0867 ,  B01L2300/0887 ,  B01L2300/161 ,  G01N33/5304 ,  G01N33/80

Abstract: Microfluidic cartridges for agglutination reactions are provided. The cartridges include a microfluidic reaction channel with at least two intake channels (1,2), one for an antigen-containing fluid and the other for an antibody-containing fluid, conjoined to a reaction channel (7) modified by incorporation of a downstream flow control channel (10). At low Reynolds Number, the two input streams layer one on top of the other in the reaction channel and form a flowing, unmixed horizontally-stratified laminar fluid diffusion (HLFD) interface for an extended duration of reaction. Surprisingly, the design, surface properties, and flow regime of microfluidic circuits of the present invention potentiate detection of antibody mediated agglutination at the stratified interface. Antigen: antibody reactions involving agglutination potentiated by these devices are useful in blood typing, in crossmatching for blood transfusion, and in immunodiagnostic agglutination assays, for example.

Abstract translation: 提供用于凝集反应的微流控盒。 药筒包括具有至少两个进气通道（1,2）的微流体反应通道，一个用于含抗原的流体，另一个用于含抗体的流体，结合到通过并入下游的反应通道（7） 流量控制通道（10）。 在低雷诺数下，两个输入流在反应通道中层叠在另一个之上，并形成流动的，未混合的水平分层的层状流体扩散（HLFD）界面，持续延长的反应时间。 令人惊奇的是，本发明的微流体回路的设计，表面性质和流动状态增强了在分层界面处的抗体介导的凝集的检测。 抗原：涉及通过这些装置增强的凝集的抗体反应可用于血型，输血交配和免疫诊断凝集试验。

公开(公告)号：WO2011094577A2

公开(公告)日：2011-08-04

申请号：PCT/US2011/022973

申请日：2011-01-28

Applicant: MICRONICS, INC. ,  BATTRELL, C., Frederick ,  SPRAGUE, Isaac ,  BRAGD, Matthew, Scott ,  CAPODANNO, Jason

Inventor： BATTRELL, C., Frederick ,  SPRAGUE, Isaac ,  BRAGD, Matthew, Scott ,  CAPODANNO, Jason

IPC: B01L3/00

CPC classification number: B01L3/50273 ,  B01L3/52 ,  B01L7/52 ,  B01L7/525 ,  B01L2200/0684 ,  B01L2200/14 ,  B01L2200/16 ,  B01L2300/044 ,  B01L2300/0672 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/087 ,  B01L2300/0887 ,  B01L2300/123 ,  B01L2400/0481 ,  B01L2400/0487 ,  B01L2400/0638 ,  G01N21/03 ,  G01N21/0332 ,  G01N21/17 ,  G01N21/64 ,  G01N21/6428 ,  Y10T137/0318

Abstract: A microfluidic cartridge and methods for performing a diagnostic, molecular or biochemical assay thereon, where all dried and/or liquid reagents necessary for the assay are contained in the cartridge and the assay requires only the addition of sample. Pneumohydraulic features, chamber and diaphragm technologies are introduced for overcoming the problems of bubble interference and reagent washout during operation of a microfluidic cartridge. The cartridges are inserted into a host instrument for performance of an assay and the cartridge is supplied as a consumable.

Abstract translation: 微流体筒和用于在其上进行诊断，分子或生物化学测定的方法，其中测定所需的全部干燥和/或液体试剂包含在筒中，并且测定仅需要添加样品 。 为了克服微流体滤芯操作期间气泡干扰和试剂冲洗的问题，引入了气液压特征，腔室和隔膜技术。 将墨盒插入主机仪器进行化验，并将墨盒作为消耗品提供。

公开(公告)号：WO2010120786A1

公开(公告)日：2010-10-21

申请号：PCT/US2010/030901

申请日：2010-04-13

Applicant: MICRONICS, INC. ,  SPRAGUE, Isaac ,  EMSWILER, John, E. ,  BATTRELL, C., Frederick ,  HAAB, Joan ,  PENNELL, Sean, M. ,  KAY, Justin, L. ,  MILLER, Zane, B. ,  DAIBER, Troy, D.

Inventor： SPRAGUE, Isaac ,  EMSWILER, John, E. ,  BATTRELL, C., Frederick ,  HAAB, Joan ,  PENNELL, Sean, M. ,  KAY, Justin, L. ,  MILLER, Zane, B. ,  DAIBER, Troy, D.

IPC: B01L3/00 ,  G01N33/52

CPC classification number: B01L3/502 ,  B01L3/5025 ,  B01L2300/0864 ,  B01L2400/049 ,  G01N33/52 ,  Y10T436/25

Abstract: A microfluidic cartridge including on-board dry reagents and microfluidic circuitry for determining a clinical analyte or analytes from a few microliters of liquid sample; with docking interface for use in a host workstation, the workstation including a pneumatic fluid controller and spectrophotometer for monitoring analytical reactions in the cartridge.

Abstract translation: 一种微流控柱，包括板上干试剂和微流体电路，用于确定临床分析物或几微升液体样品的分析物; 具有用于主机工作站的对接界面，工作站包括气动流体控制器和分光光度计，用于监测盒中的分析反应。

公开(公告)号：WO2011094577A3

公开(公告)日：2011-11-03

申请号：PCT/US2011022973

申请日：2011-01-28

Applicant: MICRONICS INC ,  BATTRELL C FREDERICK ,  SPRAGUE ISAAC ,  BRAGD MATTHEW SCOTT ,  CAPODANNO JASON

Inventor： BATTRELL C FREDERICK ,  SPRAGUE ISAAC ,  BRAGD MATTHEW SCOTT ,  CAPODANNO JASON

IPC: B01L3/00

CPC classification number: B01L3/50273 ,  B01L3/52 ,  B01L7/52 ,  B01L7/525 ,  B01L2200/0684 ,  B01L2200/14 ,  B01L2200/16 ,  B01L2300/044 ,  B01L2300/0672 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/087 ,  B01L2300/0887 ,  B01L2300/123 ,  B01L2400/0481 ,  B01L2400/0487 ,  B01L2400/0638 ,  G01N21/03 ,  G01N21/0332 ,  G01N21/17 ,  G01N21/64 ,  G01N21/6428 ,  Y10T137/0318

Abstract: A microfluidic cartridge and methods for performing a diagnostic, molecular or biochemical assay thereon, where all dried and/or liquid reagents necessary for the assay are contained in the cartridge and the assay requires only the addition of sample. Pneumohydraulic features, chamber and diaphragm technologies are introduced for overcoming the problems of bubble interference and reagent washout during operation of a microfluidic cartridge. The cartridges are inserted into a host instrument for performance of an assay and the cartridge is supplied as a consumable.

Abstract translation: 微流控筒和用于在其上进行诊断，分子或生物化学测定的方法，其中所述测定所需的所有干燥和/或液体试剂都包含在所述盒中，并且所述测定仅需添加样品。 引入气动液压特性，腔室和隔膜技术，以克服在微流控筒操作期间气泡干扰和试剂冲洗的问题。 将盒式磁盘插入到主仪器中以进行测定，并且将盒作为消耗品提供。

公开(公告)号：WO2010144683A2

公开(公告)日：2010-12-16

申请号：PCT/US2010/038141

申请日：2010-06-10

Applicant: MICRONICS, INC. ,  BATTRELL, C., Frederick ,  HOEKSTRA, Denise Maxine ,  HAAB, Joan ,  WILLIFORD, John R. ,  GATES, Sabrina N. ,  LEBER, Elizabeth R. ,  SPRAGUE, Isaac

Inventor： BATTRELL, C., Frederick ,  HOEKSTRA, Denise Maxine ,  HAAB, Joan ,  WILLIFORD, John R. ,  GATES, Sabrina N. ,  LEBER, Elizabeth R. ,  SPRAGUE, Isaac

IPC: C12Q1/68

CPC classification number: C12Q1/686 ,  C12Q1/6806 ,  C12Q2527/125

Abstract: Manufacturing methods and compositions are described for production of self-contained microfluidic cartridge devices with on-board reagents for molecular biological testing. Sensitive reagents are stored in dry form without lyophilization or freezing, and reconstituted at the point of use with either a biological sample or a sample eluate at the point of use. Manufacturing methods include sheet and roll fabrication processes where the reagents are printed in place and sealed within individual microfluidic cartridges before gel vitrification.

Abstract translation: 描述制造方法和组合物用于生产具有用于分子生物学测试的载体试剂的自含微流控盒装置。 敏感试剂以干燥形式储存而不进行冻干或冷冻，并在使用时与生物样品或样品洗脱液在使用时重构。 制造方法包括片和卷制造方法，其中将试剂印刷在适当位置并在凝胶玻璃化之前在单个微流体盒内密封。