公开(公告)号：WO2012033842A2

公开(公告)日：2012-03-15

申请号：PCT/US2011/050704

申请日：2011-09-07

Applicant: PRESIDENT AND FELLOWS OF HARVARD COLLEGE ,  HAYWORTH, Kenneth, Jeffrey ,  SCHALEK, Richard ,  TAPIA, Juan, Carlos ,  KASTHURI, Narayanan ,  LICHTMAN, Jeff

Inventor： HAYWORTH, Kenneth, Jeffrey ,  SCHALEK, Richard ,  TAPIA, Juan, Carlos ,  KASTHURI, Narayanan ,  LICHTMAN, Jeff

IPC: G01N35/00 ,  G01N1/06 ,  G01N33/48

CPC classification number: G01N1/06 ,  A61B16/00 ,  G01N35/00009 ,  G01N2001/065 ,  G01N2001/066

Abstract: Methods, apparatuses and systems for facilitating automated or semi- automated collection of tissue samples cut by a microtome. In one example, a collection apparatus may be moved back and forth between respective positions at which the collection apparatus is operatively coupled to a microtome so as to collect cut tissue samples, or routine access to the microtome is provided. Relatively easy movement and positioning of the collection apparatus is facilitated, while at the same time ensuring structural stability and appropriate alignment and/or isolation between the collection apparatus and the microtome. A fluid reservoir receives samples cut by the microtome, and the collection apparatus may collect samples via a conveyor-like substrate disposed near/in the reservoir. A linear movement of the substrate may be controlled based on a cutting rate of the microtome, and the fluid level in the reservoir may be automatically maintained to facilitate effective sample collection.

Abstract translation: 用于促进由切片机切割的组织样品的自动或半自动收集的方法，装置和系统。 在一个示例中，收集装置可以在收集装置可操作地耦合到切片机的相应位置之间来回移动，以便收集切割的组织样本，或者提供对切片机的常规访问。 促进收集装置的相对容易的移动和定位，同时确保收集装置和切片机之间的结构稳定性和适当的对准和/或隔离。 流体储存器接收由切片机切割的样品，并且收集装置可以经由设置在储存器附近/处的储存器中的输送器状基板收集样品。 可以基于切片机的切割速度来控制基底的线性移动，并且可以自动维持储存器中的液体水平以促进有效的样品收集。

公开(公告)号：WO2008066846A3

公开(公告)日：2008-12-11

申请号：PCT/US2007024507

申请日：2007-11-28

Applicant: HARVARD COLLEGE ,  HAYWORTH KENNETH ,  KASTHURI NARAYANAN ,  LICHTMAN JEFF ,  HARTWIEG ERIKA

Inventor： HAYWORTH KENNETH ,  KASTHURI NARAYANAN ,  LICHTMAN JEFF ,  HARTWIEG ERIKA

IPC: G01N1/06

CPC classification number: G01N1/06 ,  G01N1/312 ,  G01N2001/066 ,  G01N2001/362 ,  H01J2237/201 ,  H01J2237/202 ,  H01J2237/26 ,  Y10T83/04 ,  Y10T83/141

Abstract: Methods and apparatus for providing and processing serial tissue sections. In one example, an "automatic tape collecting lathe ultramicrotome" (ATLUM) slices a block of tissue sample having various geometries into a continuous ribbon of thin tissue, or multiple thin tissue sections, and disposes the sliced thin tissue on an appropriate substrate to facilitate subsequent imaging of the sliced thin tissue. Closed-loop control of section thickness of the sliced thin tissue sections or ribbons is implemented to produce thinner sliced tissue sections or ribbons and tightly regulate thickness. Thin tissue sections or ribbons may be particularly processed/prepared to facilitate imaging with a scanning electron microscope (SEM). Collected thin tissue sections or ribbons may be used to create UltraThin Section Libraries (UTSLs) that allow for fully automated, time-efficient imaging in the SEM to faciliate expansive tissue studies.

Abstract translation: 用于提供和处理系列组织切片的方法和设备。 在一个示例中，“自动带收集车床超微切片机”（ATLUM）将具有各种几何形状的组织样本块切成薄组织或多个薄组织切片的连续带，并将切成薄片的组织放置在适当的基底上以促进 随后对切片的薄组织进行成像。 实施切片薄组织切片或条带的切片厚度的闭环控制以产生更薄的切片组织切片或条带并严格调节厚度。 可以特别处理/制备薄组织切片或带以促进用扫描电子显微镜（SEM）成像。 收集的薄组织切片或色带可用于创建UltraThin部分文库（UTSLs），以便在SEM中实现全自动，省时的成像，以促进膨胀组织研究。

公开(公告)号：WO1997040983A1

公开(公告)日：1997-11-06

申请号：PCT/US1997007319

申请日：1997-04-30

Applicant: BURNS, Marshall ,  HAYWORTH, Kenneth, J. ,  MCGINNIS, Kim, F.

IPC: B32B31/00

CPC classification number: B32B38/185 ,  B22F3/005 ,  B22F2998/00 ,  B29C64/141 ,  B29C64/40 ,  B31D5/00 ,  B32B2038/1891 ,  B33Y10/00 ,  Y10T156/1064 ,  Y10T156/1075 ,  Y10T156/1082 ,  Y10T156/1105 ,  Y10T156/13 ,  Y10T156/1322 ,  Y10T156/1761 ,  Y10T156/1906 ,  B22F3/008

Abstract: A method and apparatus automatically fabricates a three-dimensional object (10) from individual layers of fabrication material (11) having a predetermined configuration. Successive layers are stacked in a predetermined sequence and affixed together to form the object (10). The fabrication material is carried on a substrate (13) to a stacker (80). At the stacker (80) the layers are stacked together, with each layer being successively affixed to the stack (66) of previously affixed layers, and with the substrate (13) removed from each layer after it is affixed.

Abstract translation: 方法和装置从具有预定构造的制造材料（11）的各层自动制造三维物体（10）。 连续层以预定顺序堆叠并固定在一起以形成物体（10）。 制造材料承载在基板（13）上至堆垛机（80）上。 在堆垛机（80）处，层叠在一起，每层依次固定到预先固定的层的叠层（66）上，并且在粘贴基板（13）之后，每层除去。

公开(公告)号：WO2012033842A3

公开(公告)日：2012-06-14

申请号：PCT/US2011050704

申请日：2011-09-07

Applicant: HARVARD COLLEGE ,  HAYWORTH KENNETH JEFFREY ,  SCHALEK RICHARD ,  TAPIA JUAN CARLOS ,  KASTHURI NARAYANAN ,  LICHTMAN JEFF

Inventor： HAYWORTH KENNETH JEFFREY ,  SCHALEK RICHARD ,  TAPIA JUAN CARLOS ,  KASTHURI NARAYANAN ,  LICHTMAN JEFF

IPC: G01N35/00 ,  G01N1/06 ,  G01N33/48

CPC classification number: G01N1/06 ,  A61B16/00 ,  G01N35/00009 ,  G01N2001/065 ,  G01N2001/066

Abstract: Methods, apparatuses and systems for facilitating automated or semi- automated collection of tissue samples cut by a microtome. In one example, a collection apparatus may be moved back and forth between respective positions at which the collection apparatus is operatively coupled to a microtome so as to collect cut tissue samples, or routine access to the microtome is provided. Relatively easy movement and positioning of the collection apparatus is facilitated, while at the same time ensuring structural stability and appropriate alignment and/or isolation between the collection apparatus and the microtome. A fluid reservoir receives samples cut by the microtome, and the collection apparatus may collect samples via a conveyor-like substrate disposed near/in the reservoir. A linear movement of the substrate may be controlled based on a cutting rate of the microtome, and the fluid level in the reservoir may be automatically maintained to facilitate effective sample collection.

Abstract translation: 用于促进由切片机切割的组织样品的自动或半自动收集的方法，设备和系统。 在一个示例中，收集装置可以在收集装置可操作地连接到切片机的相应位置之间来回移动，以收集切割的组织样本，或者提供对切片机的常规访问。 便于收集装置的相对容易的移动和定位，同时确保收集装置和切片机之间的结构稳定性和适当的对准和/或隔离。 流体储存器接收由切片机切割的样本，并且收集设备可以经由设置在储存器附近/中的传送带状基底来收集样本。 可以基于切片机的切割速率来控制基板的线性移动，并且可以自动保持储存器中的液位以促进有效的样品收集。

公开(公告)号：WO2008066846A2

公开(公告)日：2008-06-05

申请号：PCT/US2007/024507

申请日：2007-11-28

Applicant: PRESIDENT AND FELLOWS OF HARVARD COLLEGE ,  HAYWORTH, Kenneth ,  KASTHURI, Narayanan ,  LICHTMAN, Jeff ,  HARTWIEG, Erika

Inventor： HAYWORTH, Kenneth ,  KASTHURI, Narayanan ,  LICHTMAN, Jeff ,  HARTWIEG, Erika

IPC: G01N1/06

CPC classification number: G01N1/06 ,  G01N1/312 ,  G01N2001/066 ,  G01N2001/362 ,  H01J2237/201 ,  H01J2237/202 ,  H01J2237/26 ,  Y10T83/04 ,  Y10T83/141

Abstract: Methods and apparatus for providing and processing serial tissue sections. In one example, an "automatic tape collecting lathe ultramicrotome" (ATLUM) slices a block of tissue sample having various geometries into a continuous ribbon of thin tissue, or multiple thin tissue sections, and disposes the sliced thin tissue on an appropriate substrate to facilitate subsequent imaging of the sliced thin tissue. Closed-loop control of section thickness of the sliced thin tissue sections or ribbons is implemented to produce thinner sliced tissue sections or ribbons and tightly regulate thickness. Thin tissue sections or ribbons may be particularly processed/prepared to facilitate imaging with a scanning electron microscope (SEM). Collected thin tissue sections or ribbons may be used to create UltraThin Section Libraries (UTSLs) that allow for fully automated, time-efficient imaging in the SEM to faciliate expansive tissue studies.

Abstract translation: 用于提供和处理连续组织切片的方法和装置。 在一个示例中，“自动收集车床超薄切片机”（ATLUM）将具有各种几何形状的组织样品块切割成薄组织或多个薄组织切片的连续带，并将切片的薄组织置于合适的基底上以便于 切片薄组织的随后成像。 实施切片的薄组织切片或带的切片厚度的闭环控制以产生更薄的切片的组织切片或丝带并且紧密地调节厚度。 薄组织切片或带可以特别加工/制备，以便于用扫描电子显微镜（SEM）进行成像。 收集的薄组织切片或条带可用于创建UltraThin Section Libraries（UTSL），允许在SEM中进​​行全自动化，时间有效的成像，以促进膨胀性组织研究。

公开(公告)号：WO2008066846A8

公开(公告)日：2008-08-07

申请号：PCT/US2007024507

申请日：2007-11-28

Applicant: HARVARD COLLEGE ,  HAYWORTH KENNETH ,  KASTHURI NARAYANAN ,  LICHTMAN JEFF ,  HARTWIEG ERIKA

Inventor： HAYWORTH KENNETH ,  KASTHURI NARAYANAN ,  LICHTMAN JEFF ,  HARTWIEG ERIKA

IPC: G01N1/06

CPC classification number: G01N1/06 ,  G01N1/312 ,  G01N2001/066 ,  G01N2001/362 ,  H01J2237/201 ,  H01J2237/202 ,  H01J2237/26 ,  Y10T83/04 ,  Y10T83/141

Abstract: Methods and apparatus for providing and processing serial tissue sections. In one example, an "automatic tape collecting lathe ultramicrotome" (ATLUM) slices a block of tissue sample having various geometries into a continuous ribbon of thin tissue, or multiple thin tissue sections, and disposes the sliced thin tissue on an appropriate substrate to facilitate subsequent imaging of the sliced thin tissue. Closed-loop control of section thickness of the sliced thin tissue sections or ribbons is implemented to produce thinner sliced tissue sections or ribbons and tightly regulate thickness. Thin tissue sections or ribbons may be particularly processed/prepared to facilitate imaging with a scanning electron microscope (SEM). Collected thin tissue sections or ribbons may be used to create UltraThin Section Libraries (UTSLs) that allow for fully automated, time-efficient imaging in the SEM to faciliate expansive tissue studies.

Abstract translation: 用于提供和处理连续组织切片的方法和装置。 在一个示例中，“自动收集车床超薄切片机”（ATLUM）将具有各种几何形状的组织样品块切割成薄组织或多个薄组织切片的连续带，并将切片的薄组织置于合适的基底上以便于 切片薄组织的随后成像。 实施切片的薄组织切片或带的切片厚度的闭环控制以产生更薄的切片的组织切片或丝带并且紧密地调节厚度。 薄组织切片或带可以特别加工/制备，以便于用扫描电子显微镜（SEM）进行成像。 收集的薄组织切片或条带可用于创建UltraThin Section Libraries（UTSL），允许在SEM中进​​行全自动化，时间有效的成像，以促进膨胀性组织研究。