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1.发明授权Method for increasing clinical specificity when detecting tumors and their precursor stages by simultaneously measuring at least two different molecular markers 失效通过同时测量至少两种不同的分子标记物来检测肿瘤及其前体阶段时增加临床特异性的方法公开(公告)号：US07452727B2
公开(公告)日：2008-11-18
申请号：US10023501
申请日：2001-12-17
Applicant: Guido Hennig , Ralph Wirtz , Kerstin Bohmann , Birge Schopper
Inventor： Guido Hennig , Ralph Wirtz , Kerstin Bohmann , Birge Schopper
CPC classification number: G01N33/574 , A61B5/0059 , A61B5/415 , G01N1/31 , G01N15/1459 , G01N15/1463 , G01N21/6428 , G01N21/6458 , G01N2001/2873 , G01N2015/1006 , G01N2035/00356 , Y10S436/813 , Y10T436/13
Abstract: The present invention relates to an automatable method for the improved diagnosis of pathologically altered cells by simultaneously staining at least two different molecular markers, which exhibit a change in gene expression which is disease-associated, in a cell or constituent regions of a tissue sample by means of using combinations of antibodies and accrediting signal intensities.
Abstract translation: 本发明涉及一种可自动化方法，用于通过在组织样品的细胞或组成区域中同时染色至少两种不同的分子标记物，其在组织样品的细胞或组成区域中表现出与疾病相关的基因表达的变化，从而改进病理改变的细胞的诊断 使用抗体组合和认证信号强度的手段。
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公开(公告)号：US07713624B2
公开(公告)日：2010-05-11
申请号：US10554765
申请日：2004-04-29
Applicant: Christiane Meyer , Markus Haase , Werner Hoheisel , Kerstin Bohmann
Inventor： Christiane Meyer , Markus Haase , Werner Hoheisel , Kerstin Bohmann
CPC classification number: C09K11/7777 , C09K11/02 , G01N21/6428 , G01N2021/6432 , Y10S977/773 , Y10S977/834 , Y10S977/89 , Y10S977/896 , Y10S977/90 , Y10T428/25 , Y10T428/256 , Y10T428/2991
Abstract: Luminescent inorganic nanoparticles comprising: (a) a core made from a first metal salt or oxide being surrounded by (b) a shell made from a second metal salt or oxide being luminescent and having non-semiconductor properties. These nanoparticles can be advantageously used in (fluorescence) resonance energy transfer ((F)RET)-based bioassays in view of their higher (F)RET efficiency.
Abstract translation: 发光无机纳米颗粒包括：（a）由第一金属盐或氧化物制成的芯被（b）由发光的第二金属盐或氧化物制成并具有非半导体性质的壳包围。 考虑到它们的更高（F）RET效率，这些纳米颗粒可以有利地用于（荧光）共振能量转移（（F）RET）的生物测定中。
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公开(公告)号：US20070087195A1
公开(公告)日：2007-04-19
申请号：US10554765
申请日：2004-04-29
Applicant: Christiane Meyer , Markus Haase , Werner Hoheisel , Kerstin Bohmann
Inventor： Christiane Meyer , Markus Haase , Werner Hoheisel , Kerstin Bohmann
CPC classification number: C09K11/7777 , C09K11/02 , G01N21/6428 , G01N2021/6432 , Y10S977/773 , Y10S977/834 , Y10S977/89 , Y10S977/896 , Y10S977/90 , Y10T428/25 , Y10T428/256 , Y10T428/2991
Abstract: The present invention relates to luminescent inorganic nanoparticles comprising (a) a core made from a first metal salt or oxide being surrounded by (b) a shell made from a second metal salt or oxide being luminescent and having non-semiconductor properties. These particles can be advantageously used in (fluorescence) resonance energy transfer ((F)RET)-based bioassays in view of their higher (F)RET efficiency.
Abstract translation: 本发明涉及发光无机纳米颗粒，其包含（a）由第一金属盐或氧化物制成的芯，被（b）由发光的第二金属盐或氧化物制成的壳体包围，并具有非半导体性质。 鉴于它们的（F）RET效率更高，这些颗粒可以有利地用于（荧光）共振能量转移（（F）RET）的生物测定中。
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公开(公告)号：US07344895B2
公开(公告)日：2008-03-18
申请号：US10540168
申请日：2003-12-06
Applicant: Burkhard Köhler , Kerstin Bohmann , Werner Hoheisel , Markus Haase , Stefan Haubold , Christiane Meyer , Thorsten Heidelberg
Inventor： Burkhard Köhler , Kerstin Bohmann , Werner Hoheisel , Markus Haase , Stefan Haubold , Christiane Meyer , Thorsten Heidelberg
IPC: G01N33/543 , G01N37/00 , G01N33/24
CPC classification number: C09K11/7797 , C07F9/4009 , C09K11/7708 , C09K11/7712 , C09K11/7723 , C09K11/7725 , C09K11/7733 , C09K11/7734 , C09K11/7736 , C09K11/7737 , C09K11/7739 , C09K11/774 , C09K11/7741 , C09K11/7776 , C09K11/778 , C09K11/7792 , C09K11/7794 , Y10S977/81 , Y10S977/811 , Y10T436/13
Abstract: A process for synthesizing nanoparticles, in particular metal salt nanoparticles. To the synthesis mixture is added a modifying reagent which binds, by means of a first functional group, to the nanoparticle surface and which carries a second functional group for binding to molecules which are specifically selected in dependence on the subsequent use of the nanoparticles. This dispenses with a postsynthetic, separate, application-specific modification step. A new substance class, the pentaalkyl iminobis(methylenephosphono)carboxylates, are particularly suitable for this purpose. These modifying reagents permit the nanoparticles to grow in a specifically controlled manner and, at the same time, modify the surface of the growing nanoparticles, (in-situ) during the synthesis, such that the particles can be very readily dissolved in a large number of solvents and carry functional groups for coupling on molecules, resulting in the particles having, immediately after having been synthesized, a certain all-round usability.
Abstract translation: 一种合成纳米颗粒的方法，特别是金属盐纳米颗粒。 向合成混合物中加入一种修饰试剂，其通过第一官能团结合到纳米颗粒表面，并且其携带第二官能团以结合分子，其依赖于随后使用纳米颗粒而被特别选择。 这不需要一个合成后的，独立的应用程序特定的修改步骤。 新物质类，五烷基亚氨基双（亚甲基膦酰基）羧酸酯特别适合于此目的。 这些改性试剂允许纳米颗粒以特定控制的方式生长，并且同时在合成期间（原位）修饰生长的纳米颗粒的表面，使得颗粒可以非常容易地溶解在大量中 的溶剂并携带用于在分子上偶联的官能团，导致颗粒在合成后立即具有一定的全面可用性。
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Patent Agency Ranking
5.发明申请公开(公告)号：US20110092691A1
公开(公告)日：2011-04-21
申请号：US12997903
申请日：2009-06-18
Applicant: Heike Euting , Guido Hennig , Kerstin Bohmann
Inventor： Heike Euting , Guido Hennig , Kerstin Bohmann
CPC classification number: C12N15/1013
Abstract: The invention relates to a method for filtering nucleic acids, to a kit for carrying out the method according to the invention and to a novel use of magnetic particles for filtering a biological sample. The method according to the invention comprises the following steps: a) the sample is held in an aqueous solution; b) lysing of the sample; c) separation of cellular debris; and d) the nucleic acids are isolated from the solution, steps (a) to (c) taking place under non-chaotropic conditions.
Abstract translation: 本发明涉及一种过滤核酸的方法，用于实施本发明方法的试剂盒以及用于过滤生物样品的磁性颗粒的新用途。 根据本发明的方法包括以下步骤：a）将样品保持在水溶液中; b）裂解样品; c）分离细胞碎片; 和d）从溶液中分离核酸，步骤（a）至（c）在非离液性条件下进行。
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公开(公告)号：US07410810B2
公开(公告)日：2008-08-12
申请号：US10494390
申请日：2002-11-04
Applicant: Kerstin Bohmann , Werner Hoheisel , Burkhard Köhler , Ingmar Dorn
Inventor： Kerstin Bohmann , Werner Hoheisel , Burkhard Köhler , Ingmar Dorn
IPC: G01N33/551
CPC classification number: B82Y30/00 , B01J2219/005 , B01J2219/00576 , B01J2219/00585 , B01J2219/00596 , B01J2219/00722 , B01J2219/00725 , C12Q1/6818 , C40B40/06 , C40B40/10 , G01N33/542 , C12Q2563/155 , C12Q2545/101
Abstract: The invention relates to an assay based on resonance energy transfer (RET), comprising a first molecule grope A, which is marked with at least one energy donor, and at least one second molecule group B which is marked with at least one energy acceptor, the donor comprising a molecule or particle which can be energetically excited by an external radiation source and which is fluorescence enabled and the acceptor comprising a molecule or particle which can be excited by energy transfer via the donor with partial or complete quenching of the donor fluorescence, and the donor and/or acceptor comprise luminescing inorganic dope nanoparticles having an expansion of ≦50 nanometers, emitting electromagnetic radiation with stokes or anti-stokes scattering after energetic excitation.
Abstract translation: 本发明涉及基于共振能量转移（RET）的测定法，其包括用至少一个能量给体标记的第一分子曲线A和至少一个第二分子组B，其被至少一个能量受体标记， 所述供体包含能够被外部辐射源能量激发并且是荧光使能的分子或颗粒，并且所述受体包含分子或颗粒，所述分子或颗粒可以通过供体荧光的部分或完全猝灭通过供体通过能量转移而被激发， 供体和/或受体包括具有<= 50纳米的扩张的发射无机掺杂纳米颗粒，在激发激发后发射具有斯托克斯或反斯托克斯散射的电磁辐射。
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公开(公告)号：US20060063155A1
公开(公告)日：2006-03-23
申请号：US10540168
申请日：2003-12-06
Applicant: Burkhard Kohler , Kerstin Bohmann , Werner Hoheisel , Markus Haase , Stefan Haubold , Christiane Meyer , Thorsten Heidelberg
Inventor： Burkhard Kohler , Kerstin Bohmann , Werner Hoheisel , Markus Haase , Stefan Haubold , Christiane Meyer , Thorsten Heidelberg
IPC: C12Q1/68 , C01F17/00 , G01N33/551
CPC classification number: C09K11/7797 , C07F9/4009 , C09K11/7708 , C09K11/7712 , C09K11/7723 , C09K11/7725 , C09K11/7733 , C09K11/7734 , C09K11/7736 , C09K11/7737 , C09K11/7739 , C09K11/774 , C09K11/7741 , C09K11/7776 , C09K11/778 , C09K11/7792 , C09K11/7794 , Y10S977/81 , Y10S977/811 , Y10T436/13
Abstract: A process for synthesizing nanoparticles, in particular metal salt nanoparticles. To the synthesis mixture is added a modifying reagent which binds, by means of a first functional group, to the nanoparticle surface and which carries a second functional group for binding to molecules which are specifically selected in dependence on the subsequent use of the nanoparticles. This dispenses with a postsynthetic, separate, application-specific modification step. A new substance class, the pentaalkyl iminobis(methylenephosphono) carboxylates, are particularly suitable for this purpose. These modifying reagents permit the nanoparticles to grow in a specifically controlled manner and, at the same time, modify the surface of the growing nanoparticles, (in-situ) during the synthesis, such that the particles can be very readily dissolved in a large number of solvents and carry functional groups for coupling on molecules, resulting in the particles having, immediately after having been synthesized, a certain all-round usability.
Abstract translation: 一种合成纳米颗粒的方法，特别是金属盐纳米颗粒。 向合成混合物中加入一种修饰试剂，其通过第一官能团结合到纳米颗粒表面，并且其携带第二官能团以结合分子，其依赖于随后使用纳米颗粒而被特别选择。 这不需要一个合成后的，独立的应用程序特定的修改步骤。 新物质类，五烷基亚氨基双（亚甲基膦酰基）羧酸酯特别适合于此目的。 这些改性试剂允许纳米颗粒以特定控制的方式生长，并且同时在合成期间（原位）修饰生长的纳米颗粒的表面，使得颗粒可以非常容易地溶解在大量中 的溶剂并携带用于在分子上偶联的官能团，导致颗粒在合成后立即具有一定的全面可用性。
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公开(公告)号：US20050064604A1
公开(公告)日：2005-03-24
申请号：US10494390
申请日：2002-11-04
Applicant: Kerstin Bohmann , Werner Hoheisel , Burkhard Kohler , Ingmar Dorn
Inventor： Kerstin Bohmann , Werner Hoheisel , Burkhard Kohler , Ingmar Dorn
IPC: G01N33/543 , B82B20060101 , B82B1/00 , C12Q1/34 , C12Q1/37 , C12Q1/42 , C12Q1/48 , C12Q1/68 , C40B40/06 , C40B40/10 , G01N21/64 , G01N21/78 , G01N33/52 , G01N33/53 , G01N33/542 , G01N33/553 , G01N33/566 , G01N33/58 , G01N33/60
CPC classification number: B82Y30/00 , B01J2219/005 , B01J2219/00576 , B01J2219/00585 , B01J2219/00596 , B01J2219/00722 , B01J2219/00725 , C12Q1/6818 , C40B40/06 , C40B40/10 , G01N33/542 , C12Q2563/155 , C12Q2545/101
Abstract: The invention relates to an assay based on resonance energy transfer (RET), comprising a first molecule grope A, which is marked with at least one energy donor, and at least one second molecule group B which is marked with at least one energy acceptor, the donor comprising a molecule or particle which can be energetically excited by an external radiation source and which is fluorescence enabled and the acceptor comprising a molecule or particle which can be excited by energy transfer via the donor with partial or complete quenching of the donor fluorescence, and the donor and/or acceptor comprise luminescing inorganic dope nanoparticles having an expansion of ≦50 nanometers, emitting electromagnetic radiation with stokes or anti-stokes scattering after energetic excitation.
Abstract translation: 本发明涉及基于共振能量转移（RET）的测定法，其包括用至少一个能量给体标记的第一分子曲线A和至少一个第二分子组B，其被至少一个能量受体标记， 所述供体包含能够被外部辐射源能量激发并且是荧光使能的分子或颗粒，并且所述受体包含分子或颗粒，所述分子或颗粒可以通过供体荧光的部分或完全猝灭通过供体通过能量转移而被激发， 供体和/或受体包括具有<= 50纳米的扩张的发射无机掺杂纳米颗粒，在激发激发后发射具有斯托克斯或反斯托克斯散射的电磁辐射。
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公开(公告)号：US08846897B2
公开(公告)日：2014-09-30
申请号：US12997903
申请日：2009-06-18
Applicant: Heike Euting , Guido Hennig , Kerstin Bohmann
Inventor： Heike Euting , Guido Hennig , Kerstin Bohmann
CPC classification number: C12N15/1013
Abstract: The invention relates to a method for filtering nucleic acids, to a kit for carrying out the method according to the invention and to a novel use of magnetic particles for filtering a biological sample. The method according to the invention comprises the following steps: a) the sample is held in an aqueous solution; b) lysing of the sample; c) separation of cellular debris; and d) the nucleic acids are isolated from the solution, steps (a) to (c) taking place under non-chaotropic conditions.
Abstract translation: 本发明涉及一种过滤核酸的方法，用于实施本发明方法的试剂盒以及用于过滤生物样品的磁性颗粒的新用途。 根据本发明的方法包括以下步骤：a）将样品保持在水溶液中; b）裂解样品; c）分离细胞碎片; 和d）从溶液中分离核酸，步骤（a）至（c）在非离液性条件下进行。
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