公开(公告)号：WO2011143044A3

公开(公告)日：2011-11-17

申请号：PCT/US2011/035346

申请日：2011-05-05

Applicant: IDEXX LABORATORIES, INC. ,  LIN, JuiMing ,  DEKA, Chiranjit

Inventor： LIN, JuiMing ,  DEKA, Chiranjit

IPC: G01N33/48

Abstract: A lytic reagent composition and methods for differentiation and enumeration of leukocytes are disclosed. The lytic reagent is useful for rapid and automated sample preparation methods in an automated hematology analyzer, and also for manual and slow sample preparation methods required for immunophenotyping cells in a conventional fluorescence flow cytometer. The reagent system includes a nonionic surfactant in a hypotonic solution, an inorganic buffer to maintain the pH in a range of from about 6 to about 10, and optionally a leukocyte stabilizer. The reagent system is used to lyse red blood cells and stabilize the leukocytes so that antibody binding sites on the leukocytes remain unaffected by the process and the blood samples may be labeled with antibodies either before or after the lysis of the red blood cells.

公开(公告)号：WO2011143044A2

公开(公告)日：2011-11-17

申请号：PCT/US2011035346

申请日：2011-05-05

Applicant: IDEXX LAB INC ,  LIN JUIMING ,  DEKA CHIRANJIT

Inventor： LIN JUIMING ,  DEKA CHIRANJIT

IPC: C12Q1/00

CPC classification number: G01N33/537 ,  G01N33/56972

Abstract: A lytic reagent composition and methods for differentiation and enumeration of leukocytes are disclosed. The lytic reagent is useful for rapid and automated sample preparation methods in an automated hematology analyzer, and also for manual and slow sample preparation methods required for immunophenotyping cells in a conventional fluorescence flow cytometer. The reagent system includes a nonionic surfactant in a hypotonic solution, an inorganic buffer to maintain the pH in a range of from about 6 to about 10, and optionally a leukocyte stabilizer. The reagent system is used to lyse red blood cells and stabilize the leukocytes so that antibody binding sites on the leukocytes remain unaffected by the process and the blood samples may be labeled with antibodies either before or after the lysis of the red blood cells.

Abstract translation: 公开了一种裂解试剂组合物和白细胞的分化和计数方法。 溶解试剂可用于自动血液分析仪中的快速和自动化样品制备方法，以及常规荧光流式细胞仪中免疫分型细胞所需的手动和慢速样品制备方法。 试剂系统包括在低渗溶液中的非离子表面活性剂，将pH保持在约6至约10范围内的无机缓冲剂和任选的白细胞稳定剂。 试剂系统用于裂解红细胞并稳定白细胞，使得白细胞上的抗体结合位点不受该过程的影响，血液样品可以在红细胞裂解之前或之后用抗体标记。