Abstract:
Photometer having a variable wavelength comprising at least one light source, a monochromator having an adjustable wavelength, a sample position and a detector which detects the substantially monochromatic radiation obtained from the light source via the monochromator and the sample position. Furthermore, the photometer comprises a rotatable disc located in the light path and provided with angle position indication means by means of which it is possible to determine from the instant values of the detector signal the segment of the disc that has affected the radiation giving rise to the instant values in question.
Abstract:
Method for a solid phase synthesis of a linear combination of amino acid residues, linked via peptide bonds, starting with an amino acid residue covalently linked to a support and protected by an N-alpha-amino protecting group, comprising the following steps: (a) removing the N-alpha-amino protecting group to obtain an N-alpha-amino group, (b) adding an amino acid residue protected by an N-alpha-amino protecting group, via a peptide bond, to the N-alpha-amino group obtained in step (a) by use of a reactive protected amino acid derivative and, where necessary, a catalyst, (c) repeating steps (a) and (b) until the said liner combination has been obtained. According to the invention the said reactive protected amino acid derivative has the acyl group used to form the peptide bond activated as a pentafluorophenyl ester.
Abstract:
Procédé de synthèse en phase solide d'une combinaison linéaire de résidus d'acides aminés, liés par des liaisons peptides, commençant par un résidu d'acide aminé lié de manière covalente à un support et protégé par un groupe protecteur N-alpha-amino, consistant à a) éliminer le groupe protecteur N-alpha-amino pour obtenir un groupe N-alpha-amino; b) ajouter un résidu d'acide aminé protégé par un groupe protecteur N-alpha-amino, via une liaison peptide, au groupe N-alpha-amino obtenu dans l'étage a), en utilisant un dérivé d'acide aminé réactif protégé et, si nécessaire, un catalyseur; c) répéter les étapes a) et b) jusqu'à obtention de ladite combinaison linéaire. Selon l'invention, dans le dérivé d'acide aminé réactif protégé, le groupe acyle utilisé pour former la liaison peptide est activé comme un ester de pentafluorophényle.
Abstract:
Photometer having a variable wavelength comprising at least one light source, a monochromator having an adjustable wavelength, a sample position and a detector which detects the substantially monochromatic radiation obtained from the light source via the monochromator and the sample position. Furthermore, the photometer comprises a rotatable disc located in the light path and provided with angle position indication means by means of which it is possible to determine from the instant values of the detector signal the segment of the disc that has affected the radiation giving rise to the instant values in question.