公开(公告)号：WO2010039852A2

公开(公告)日：2010-04-08

申请号：PCT/US2009059059

申请日：2009-09-30

Applicant: ABBOTT LAB ,  HSIEH CHUNG-MING ,  KUTSKOVA YULIYA A ,  MEMMOTT JOHN E

Inventor： HSIEH CHUNG-MING ,  KUTSKOVA YULIYA A ,  MEMMOTT JOHN E

IPC: C12Q1/68 ,  C07H21/00 ,  C40B40/08 ,  C40B50/06

CPC classification number: C40B50/06 ,  C07K16/00 ,  C07K16/1018 ,  C07K16/241 ,  C07K16/244 ,  C07K2317/21 ,  C07K2317/622 ,  C12Q2563/185 ,  C40B40/08

Abstract: The present invention features improved in vitro RNA display libraries to allow reliable expression and selection of scFv antibody molecules from expression libraries. The scFv antibody libraries of the invention contain an optimized, shortened inter-domain linker that improves expression scFv antibody expression. The scFv antibody libraries also include short nucleic acid barcodes that allow for identification of individual library clones, libraries or subsets thereof. Primers for generating, amplifying and spectratyping the scFv antibody libraries of the invention are also provided.

Abstract translation: 本发明的特征在于改进的体外RNA显示文库以允许来自表达文库的scFv抗体分子的可靠表达和选择。 本发明的scFv抗体文库含有改善表达scFv抗体表达的优化的缩短的域间连接体。 scFv抗体文库还包括允许识别单个文库克隆，文库或其子集的短核酸条形码。 还提供了用于产生，扩增和分光本发明的scFv抗体文库的引物。

公开(公告)号：WO2008082651A3

公开(公告)日：2008-07-10

申请号：PCT/US2007/026482

申请日：2007-12-28

Applicant: ABBOTT LABORATORIES ,  HSIEH, Chung-ming ,  MCRAE, Bradford, L. ,  KUTSKOVA, Yuliya ,  MEMMOTT, John, E. ,  ROGUSKA, Michael ,  TOMLINSON, Ian ,  TUNA, Mihriban ,  GRANT, Steven ,  ENEVER, Carrie

Inventor： HSIEH, Chung-ming ,  MCRAE, Bradford, L. ,  KUTSKOVA, Yuliya ,  MEMMOTT, John, E. ,  ROGUSKA, Michael ,  TOMLINSON, Ian ,  TUNA, Mihriban ,  GRANT, Steven ,  ENEVER, Carrie

IPC: A61K39/00

Abstract: The invention provides an isolated, dual-specific antibody, or an antigen-binding portion thereof, which is specific for human IL-l α and human IL-I β. The dual specific antibodies of the invention also neutralize both human IL-I α and human IC-I β. The invention also provides domain antibodies (dAbs) specific for human IL-l α and human IL-l β.

公开(公告)号：WO2010102251A2

公开(公告)日：2010-09-10

申请号：PCT/US2010/026424

申请日：2010-03-05

Applicant: ABBOTT LABORATORIES ,  HSIEH, Chung-Ming ,  HUGUNIN, Margaret ,  MURTAZA, Anwar ,  McRAE, Bradford, L. ,  KUTSKOVA, Yuliya ,  MEMMOTT, John, E. ,  PEREZ, Jennifer, M. ,  ZHONG, Suju ,  TARCSA, Edit ,  CLABBERS, Anca ,  WALLACE, Craig ,  BRYANT, Shaughn, H. ,  LEDDY, Mary, R.

Inventor： HSIEH, Chung-Ming ,  HUGUNIN, Margaret ,  MURTAZA, Anwar ,  McRAE, Bradford, L. ,  KUTSKOVA, Yuliya ,  MEMMOTT, John, E. ,  PEREZ, Jennifer, M. ,  ZHONG, Suju ,  TARCSA, Edit ,  CLABBERS, Anca ,  WALLACE, Craig ,  BRYANT, Shaughn, H. ,  LEDDY, Mary, R.

IPC: A61K39/395

CPC classification number: C07K16/468 ,  A61K39/3955 ,  A61K2039/505 ,  A61K2039/507 ,  A61K2300/00 ,  C07K16/241 ,  C07K16/244 ,  C07K2317/21 ,  C07K2317/31 ,  C07K2317/50 ,  C07K2317/565 ,  C07K2317/64 ,  C07K2317/76 ,  C07K2317/92 ,  Y02A50/386 ,  Y02A50/41 ,  Y02A50/412 ,  Y02A50/466

Abstract: Proteins that bind IL- 17 and/or IL- 17F are described along with there use in composition and methods for treating, preventing, and diagnosing IL- 17 related diseases and for detecting IL-17 in cells, tissues, samples, and compositions.

Abstract translation: 与用于治疗，预防和诊断IL-17相关疾病和检测细胞，组织，样品和组合物中的IL-17的组合物和方法一起描述了结合IL-17和/或IL-17F的蛋白质。

公开(公告)号：WO2010039850A1

公开(公告)日：2010-04-08

申请号：PCT/US2009/059057

申请日：2009-09-30

Applicant: ABBOTT LABORATORIES ,  HSIEH, Chung-ming ,  KUTSKOVA, Yuliya, A. ,  MEMMOTT, John, E.

Inventor： HSIEH, Chung-ming ,  KUTSKOVA, Yuliya, A. ,  MEMMOTT, John, E.

IPC: G01N33/53

CPC classification number: G01N33/54326 ,  C12N15/1041

Abstract: The present invention features improved methods of in vitro RNA display to allow reliable expression and selection of scFv antibody molecules from expression libraries. The improved methods, in part, involve the use of mildly reducing conditions, which favor of scFv intra-chain disulphide bond and thus correct folding of the scFv antibody molecules. Although particularly suited to expression and selection of scFv antibody molecules, the methods of the invention are also expedient for in vitro RNA display of all classes of protein.

Abstract translation: 本发明的特征在于体外RNA显示的改进方法，以允许来自表达文库的scFv抗体分子的可靠表达和选择。 改进的方法部分地涉及使用轻度还原条件，其有利于scFv链内二硫键，从而正确折叠scFv抗体分子。 尽管特别适合于scFv抗体分子的表达和选择，本发明的方法也适用于所有类别的蛋白质的体外RNA显示。

公开(公告)号：WO2008082651A2

公开(公告)日：2008-07-10

申请号：PCT/US2007026482

申请日：2007-12-28

Applicant: ABBOTT LAB ,  HSIEH CHUNG-MING ,  MCRAE BRADFORD L ,  KUTSKOVA YULIYA ,  MEMMOTT JOHN E ,  ROGUSKA MICHAEL ,  TOMLINSON IAN ,  TUNA MIHRIBAN ,  GRANT STEVEN ,  ENEVER CARRIE

Inventor： HSIEH CHUNG-MING ,  MCRAE BRADFORD L ,  KUTSKOVA YULIYA ,  MEMMOTT JOHN E ,  ROGUSKA MICHAEL ,  TOMLINSON IAN ,  TUNA MIHRIBAN ,  GRANT STEVEN ,  ENEVER CARRIE

IPC: C07K16/24

CPC classification number: C07K16/468 ,  A61K39/3955 ,  A61K45/06 ,  C07K16/245 ,  C07K2317/569 ,  C07K2317/92

Abstract: The invention provides an isolated, dual-specific antibody, or an antigen-binding portion thereof, which is specific for human IL-l a and human IL-I ß. The dual specific antibodies of the invention also neutralize both human IL-I a and human IC-I ß. The invention also provides domain antibodies (dAbs) specific for human IL-l a and human IL-l ß.

Abstract translation: 本发明提供了对人IL-1α和人IL-1β特异的分离的双重特异性抗体或其抗原结合部分。 本发明的双重特异性抗体还中和人IL-1α和人IC-1β。 本发明还提供对人IL-1α和人IL-1β特异性的结构域抗体（dAb）。

公开(公告)号：WO2010102251A3

公开(公告)日：2010-11-25

申请号：PCT/US2010026424

申请日：2010-03-05

Applicant: ABBOTT LAB ,  HSIEH CHUNG-MING ,  HUGUNIN MARGARET ,  MURTAZA ANWAR ,  MCRAE BRADFORD L ,  KUTSKOVA YULIYA ,  MEMMOTT JOHN E ,  PEREZ JENNIFER M ,  ZHONG SUJU ,  TARCSA EDIT ,  CLABBERS ANCA ,  WALLACE CRAIG ,  BRYANT SHAUGHN H ,  LEDDY MARY R

Inventor： HSIEH CHUNG-MING ,  HUGUNIN MARGARET ,  MURTAZA ANWAR ,  MCRAE BRADFORD L ,  KUTSKOVA YULIYA ,  MEMMOTT JOHN E ,  PEREZ JENNIFER M ,  ZHONG SUJU ,  TARCSA EDIT ,  CLABBERS ANCA ,  WALLACE CRAIG ,  BRYANT SHAUGHN H ,  LEDDY MARY R

IPC: A61K39/395

CPC classification number: C07K16/468 ,  A61K39/3955 ,  A61K2039/505 ,  A61K2039/507 ,  A61K2300/00 ,  C07K16/241 ,  C07K16/244 ,  C07K2317/21 ,  C07K2317/31 ,  C07K2317/50 ,  C07K2317/565 ,  C07K2317/64 ,  C07K2317/76 ,  C07K2317/92

Abstract: Proteins that bind IL- 17 and/or IL- 17F are described along with there use in composition and methods for treating, preventing, and diagnosing IL- 17 related diseases and for detecting IL-17 in cells, tissues, samples, and compositions.

Abstract translation: 描述了结合IL-17和/或IL-17F的蛋白以及其在用于治疗，预防和诊断IL-17相关疾病和检测细胞，组织，样品和组合物中的IL-17的组合物和方法中的应用。

公开(公告)号：WO2010039852A3

公开(公告)日：2010-04-08

申请号：PCT/US2009/059059

申请日：2009-09-30

Applicant: ABBOTT LABORATORIES ,  HSIEH, Chung-Ming ,  KUTSKOVA, Yuliya, A. ,  MEMMOTT, John, E.

Inventor： HSIEH, Chung-Ming ,  KUTSKOVA, Yuliya, A. ,  MEMMOTT, John, E.

IPC: C07H21/04 ,  C40B40/08

Abstract: The present invention features improved in vitro RNA display libraries to allow reliable expression and selection of scFv antibody molecules from expression libraries. The scFv antibody libraries of the invention contain an optimized, shortened inter-domain linker that improves expression scFv antibody expression. The scFv antibody libraries also include short nucleic acid barcodes that allow for identification of individual library clones, libraries or subsets thereof. Primers for generating, amplifying and spectratyping the scFv antibody libraries of the invention are also provided.