公开(公告)号：WO2005072121A2

公开(公告)日：2005-08-11

申请号：PCT/US2005/001061

申请日：2005-01-12

Applicant: BIO-RAD LABORATORIES, INC. ,  OLECH, Lee

Inventor： OLECH, Lee

IPC: C07C279/12 ,  G01N27/447

CPC classification number: G01N27/44795 ,  C07C279/12 ,  G01N27/44747

Abstract: Carboxylic acid-substituted polyalkylene polyamines in which amine nitrogen atoms on the polyamine backbone structure are replaced by guanidine groups provide a pH range extending into high pH values. These substances are useful as carrier ampholytes in isoelectric focusing.

Abstract translation: 羧胺取代的聚亚烷基多胺，其中多胺主链结构上的胺氮原子被胍基团取代，提供了延伸到高pH值的pH范围。 这些物质可用作等电聚焦中的载体两性电解质。

公开(公告)号：WO2010027995A1

公开(公告)日：2010-03-11

申请号：PCT/US2009/055657

申请日：2009-09-02

Applicant: BIO-RAD LABORATORIES, INC. ,  ROWELL, Craig ,  PANATTONI, Cory ,  BERKELMAN, Thomas, R. ,  CATER, Sean ,  PETERSEN, Shane ,  OLECH, Lee ,  YANG, Xuemei

Inventor： ROWELL, Craig ,  PANATTONI, Cory ,  BERKELMAN, Thomas, R. ,  CATER, Sean ,  PETERSEN, Shane ,  OLECH, Lee ,  YANG, Xuemei

IPC: G01N33/558 ,  G01N33/559 ,  G01N33/561

CPC classification number: G01N27/44747 ,  B01D57/02 ,  C08F220/56 ,  C08F222/385

Abstract: Polyacrylamide gels that offer high resolution in protein separations and are more stable relative to hydrolysis than conventional polyacrylamide gels that rely on Tris or Tris-Bis as buffering agents are made by incorporating triethanolamine in place of most or all of the Tris or Tris-Bis.

Abstract translation: 聚丙烯酰胺凝胶在蛋白质分离中提供高分辨率并且相对于水解比常规聚丙烯酰胺凝胶更稳定，其依赖于Tris或Tris-Bis作为缓冲剂，通过掺入三乙醇胺代替大部分或全部Tris或Tris-Bis制备。

公开(公告)号：WO2005072121A3

公开(公告)日：2006-04-27

申请号：PCT/US2005001061

申请日：2005-01-12

Applicant: BIO RAD LABORATORIES ,  OLECH LEE

Inventor： OLECH LEE

IPC: C07C279/12 ,  G01N27/447 ,  G01N27/26 ,  C07C279/00

CPC classification number: G01N27/44795 ,  C07C279/12 ,  G01N27/44747

Abstract: Carboxylic acid-substituted polyalkylene polyamines in which amine nitrogen atoms on the polyamine backbone structure are replaced by guanidine groups provide a pH range extending into high pH values. These substances are useful as carrier ampholytes in isoelectric focusing.

Abstract translation: 羧胺取代的聚亚烷基多胺，其中多胺主链结构上的胺氮原子被胍基团取代，提供了延伸到高pH值的pH范围。 这些物质可用作等电聚焦中的载体两性电解质。

公开(公告)号：WO2005024041A3

公开(公告)日：2005-08-04

申请号：PCT/US2004029018

申请日：2004-09-02

Applicant: BIO RAD LABORATORIES ,  OLECH LEE

Inventor： OLECH LEE

IPC: C07K14/47 ,  C12Q20060101 ,  G01N33/00 ,  G01N33/20 ,  G01N33/68

CPC classification number: G01N33/6848 ,  Y10T436/142222

Abstract: Comparative studies of proteins from two or more cell populations are performed by alkylating the cysteine thiol groups of the proteins of all populations with vinyl pyridines in which the pyridine ring in at least one of the populations, in addition to the vinyl substituent, bears one or more alkyl substituents, the various populations thus differing in the alkyl substituents on the pyridine ring. Proteins of one population are thus alkylated with vinyl pyridine itself and those of another population with an alkyl-substituted vinyl pyridine, or both with alkyl-substituted vinyl pyridines using different alkyl groups or a different number of alkyl groups. The mass differential afforded by the difference in alkyl substitution affords a means of pairing individual proteins of one population with those of another while differentiating between the two, using analytical techniques disclosed in the prior art for isotope-coded affinity tagging.

Abstract translation: 来自两个或多个细胞群体的蛋白质的比较研究是通过用乙烯基吡啶烷基化所有群体的蛋白质的半胱氨酸硫醇基团进行的，其中至少一个种群中的吡啶环除了乙烯基取代基之外，还含有一个或 更多的烷基取代基，因此各种群体在吡啶环上的烷基取代基不同。 因此，一个群体的蛋白质由乙烯基吡啶本身和另一个群体与烷基取代的乙烯基吡啶烷基化，或两者使用不同烷基或不同数目的烷基烷基取代的乙烯基吡啶二烷基化。 通过烷基取代的差异提供的质量差异提供了使用现有技术中公开的用于同位素编码的亲和标记的分析技术，将一个群体的单个蛋白质与另一个群体的单个蛋白质与两者之间的差异进行分化的方法。

公开(公告)号：WO2005024041A2

公开(公告)日：2005-03-17

申请号：PCT/US2004/029018

申请日：2004-09-02

Applicant: BIO-RAD LABORATORIES, INC. ,  OLECH, Lee

Inventor： OLECH, Lee

IPC: C12Q

CPC classification number: G01N33/6848 ,  Y10T436/142222

Abstract: Comparative studies of proteins from two or more cell populations are performed by alkylating the cysteine thiol groups of the proteins of all populations with vinyl pyridines in which the pyridine ring in at least one of the populations, in addition to the vinyl substituent, bears one or more alkyl substituents, the various populations thus differing in the alkyl substituents on the pyridine ring. Proteins of one population are thus alkylated with vinyl pyridine itself and those of another population with an alkyl-substituted vinyl pyridine, or both with alkyl-substituted vinyl pyridines using different alkyl groups or a different number of alkyl groups. The mass differential afforded by the difference in alkyl substitution affords a means of pairing individual proteins of one population with those of another while differentiating between the two, using analytical techniques disclosed in the prior art for isotope-coded affinity tagging.

Abstract translation: 来自两个或更多个细胞群体的蛋白质的比较研究通过用乙烯基吡啶将所有群体的蛋白质的半胱氨酸巯基烷基化来进行，其中至少一个群体中的吡啶环 乙烯基取代基带有一个或多个烷基取代基，因此吡啶环上烷基取代基的各种群因此不同。 因此，一个群体的蛋白质用乙烯基吡啶本身烷基化，另一群体用烷基取代的乙烯基吡啶烷基化，或两个烷基取代的乙烯基吡啶用不同的烷基或不同数量的烷基烷基化。 由烷基取代差异所提供的质量差异提供了一种使一个种群的个体蛋白与另一种种群的个体蛋白配对的方法，同时使用现有技术中公开的用于同位素编码的亲和标签标记的分析技术区分二者之间的配对。