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    BACTERIAL LETHALITY TEST INDICATOR AND PROMPT RESPONSE SPECTROSCOPIC ANALYZER
    2.
    发明申请
    BACTERIAL LETHALITY TEST INDICATOR AND PROMPT RESPONSE SPECTROSCOPIC ANALYZER 审中-公开

    公开(公告)号：WO2005036128A3

    公开(公告)日：2005-04-21

    申请号：PCT/US2004/032436

    申请日：2004-10-01

    Applicant: SGM BIOTECH, INC. ,  GILLIS, John, R. ,  HICKEY, Kathleen, A. ,  HARTZE, Darlene ,  PETERSON, Douglas

    Inventor： GILLIS, John, R. ,  HICKEY, Kathleen, A. ,  HARTZE, Darlene ,  PETERSON, Douglas

    IPC: C12Q1/22

    Abstract: Multiple-component integral-structure dual-test sterility indicator (19), method of assembling physical components, selected spores (28), and chemical constituents in solution, within the indicator and arranged to enable dual-evaluations of bacterial-lethality of a dry-goods load, resulting from exposure to a selected saturated-­steam sterilization cycle. The chemical constituents are formulated to chemically produce reaction-product, which is quantitatively responsive to the combined effect of cycle steam temperature and time of exposure at that temperature, for providing a promptly-available spectroscopic evaluation of bacterial-lethality. Absorption of narrow-band selected visible-light wavelength by reaction-product, if any, of chemical-constituent solution, is used to quantitatively measure that combined-effect exposure. Subsequent to such prompt­-response evaluation of bacterial-lethality, a biological evaluation of bacterial-lethality, utilizing the confined contents of the same test indicator (19), free of risk of ambient contamination of any such contents, by evaluating pH change, if any, due to live spore-growth, or the absence thereof, following a predetermined spore incubation period, utilizing the exposed liquid constituent-solution for such spore-culturing evaluation.

    BACTERIAL LETHALITY TEST INDICATOR AND PROMPT RESPONSE SPECTROSCOPIC ANALYZER
    3.
    发明申请
    BACTERIAL LETHALITY TEST INDICATOR AND PROMPT RESPONSE SPECTROSCOPIC ANALYZER 审中-公开
    细菌色素测试指示剂和提示响应光谱分析仪

    公开(公告)号：WO2005036128A2

    公开(公告)日：2005-04-21

    申请号：PCT/US2004032436

    申请日：2004-10-01

    Applicant: SGM BIOTECH INC ,  GILLIS JOHN R ,  HICKEY KATHLEEN A ,  HARTZE DARLENE ,  PETERSON DOUGLAS

    Inventor： GILLIS JOHN R ,  HICKEY KATHLEEN A ,  HARTZE DARLENE ,  PETERSON DOUGLAS

    IPC: C12M1/34 ,  C12Q1/22 ,  G01N20060101 ,  G01N

    CPC classification number: C12Q1/22

    Abstract: Multiple-component integral-structure dual-test sterility indicator, method of assembling physical components, selected spores, and chemical constituents in solution, within the indicator and arranged to enable dual-evaluations of bacterial-lethality of a dry-goods load, resulting from exposure to a selected saturated-­steam sterilizing cycle. The chemical constituents are formulated to chemically produce reaction-product, which is quantitatively responsive to the combined effect of cycle steam temperature and time of exposure at that temperature, for providing a promptly-available spectroscopic evaluation of bacterial-lethality. Absorption of narrow-band selected visible-light wavelength by reaction-product, if any, of chemical-­constituent solution, is used to quantitatively measure that combined-effect exposure. Subsequent to such prompt­-response evaluation of bacterial-lethality, a biological evaluation of bacterial-lethality, utilizing the confined contents of the same test indicator, free of risk of ambient contamination of any such contents, by evaluating pH change, if any, due to live spore-growth, or the absence thereof, following a predetermined spore incubation period, utilizing the exposed liquid constituent-solution for such spore-culturing evaluation.

    Abstract translation: 多组分整体结构双重测试不育指示剂，组合物理成分的方法，选择的孢子和溶液中的化学成分在指示剂内，并布置成能够对干货物负载的细菌致死性进行双重评估， 暴露于选定的饱和蒸汽灭菌循环。 化学成分被配制成化学产生反应产物，其定量响应于在该温度下循环蒸汽温度和暴露时间的组合效应，以提供细菌致死率的及时可用的光谱评估。 使用化学成分溶液的反应产物（如果有的话）吸收窄带选择的可见光波长来定量测量该组合效应曝光。 在对细菌杀伤力进行这种快速反应评估之后，通过评估pH变化（如果有的话），利用相同测试指标的限制内容物，没有任何此类内容物的环境污染的风险，对细菌致死率进行生物学评估 在预定的孢子孵育期之后，利用暴露的液体成分溶液进行这样的孢子培养评价来活孢子生长或其不存在。

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