公开(公告)号：WO2006045041A2

公开(公告)日：2006-04-27

申请号：PCT/US2005/037880

申请日：2005-10-18

Applicant: THE BOARD OF TRUSTEES OF THE LELAND STANFORD JUNIOR UNIVERSITY ,  SPORMANN, Alfred, M. ,  THORMANN, Kai, M. ,  SAVILLE, Renee, M.

Inventor： SPORMANN, Alfred, M. ,  THORMANN, Kai, M. ,  SAVILLE, Renee, M.

IPC: C12M3/06

CPC classification number: C12Q1/18

Abstract: The formation and maintenance of microbial biofilms is shown to be dependent on signaling pathways mediated by cyclic di-GMP. In the absence of such signaling, microbes detach from a biofilm, and thereby become more readily treatable with conventional antibiotics. Chemical or biological means that interfere with cyclic-di-GMP signaling induce biofilm dissolution, providing for a new class of antibiotics. In one embodiment of the invention, the biofilm inhibitor is an analog of cyclic-di-GMP, which competitively or non-competitively blocks signaling. In another embodiment of the invention, the biofilm inhibitor is a genetic sequence that interferes with cyclic-di-GMP synthesis or signaling.

Abstract translation: 微生物生物膜的形成和维持显示依赖于由环状二-GMP介导的信号传导途径。 在没有这种信号的情况下，微生物与生物膜分离，从而变得更容易用常规抗生素治疗。 化学或生物意味着干扰环状二GMP信号传导诱导生物膜溶解，提供一类新型抗生素。 在本发明的一个实施方案中，生物膜抑制剂是循环二-GMP的类似物，其竞争性或非竞争性阻断信号传导。 在本发明的另一个实施方案中，生物膜抑制剂是干扰环状二-GMP合成或信号传导的遗传序列。

公开(公告)号：WO2014205146A1

公开(公告)日：2014-12-24

申请号：PCT/US2014/043053

申请日：2014-06-18

Applicant: CALYSTA, INC. ,  SAVILLE, Renee, M. ,  LEE, Sungwon ,  REGITSKY, Drew, D. ,  RESNICK, Sol, M.

Inventor： SAVILLE, Renee, M. ,  LEE, Sungwon ,  REGITSKY, Drew, D. ,  RESNICK, Sol, M. ,  SILVERMAN, Joshua

IPC: C12N9/04 ,  C12P7/56

CPC classification number: C12N9/0006 ,  C07B59/001 ,  C07B2200/05 ,  C07C59/08 ,  C12N15/74 ,  C12P7/56 ,  C12Y101/01027

Abstract: The present disclosure provides non-naturally occurring c1 microorganisms useful for the production of lactate and related compositions, as well as methods for the biologically production of lactate. In specific embodiments, the present disclosure provides non-naturally occurring methanotrophic bacteria which are useful for producing lactate from c1 substrates.

Abstract translation: 本公开提供了可用于生产乳酸盐和相关组合物的非天然存在的c1微生物以及用于生物地生产乳酸盐的方法。 在具体实施方案中，本公开提供可用于从c1底物产生乳酸盐的非天然存在的甲烷营养细菌。

公开(公告)号：WO2006045041A3

公开(公告)日：2007-04-26

申请号：PCT/US2005037880

申请日：2005-10-18

Applicant: UNIV LELAND STANFORD JUNIOR ,  SPORMANN ALFRED M ,  THORMANN KAI M ,  SAVILLE RENEE M

Inventor： SPORMANN ALFRED M ,  THORMANN KAI M ,  SAVILLE RENEE M

IPC: C12Q1/18 ,  A61K31/7064 ,  A61K31/7068 ,  A61K31/7072 ,  A61K31/7076 ,  A61K31/708 ,  A61K31/7105 ,  A61K31/711

CPC classification number: C12Q1/18

Abstract: The formation and maintenance of microbial biofilms is shown to be dependent on signaling pathways mediated by cyclic di-GMP. In the absence of such signaling, microbes detach from a biofilm, and thereby become more readily treatable with conventional antibiotics. Chemical or biological means that interfere with cyclic-di-GMP signaling induce biofilm dissolution, providing for a new class of antibiotics. In one embodiment of the invention, the biofilm inhibitor is an analog of cyclic-di-GMP, which competitively or non-competitively blocks signaling. In another embodiment of the invention, the biofilm inhibitor is a genetic sequence that interferes with cyclic-di-GMP synthesis or signaling.

Abstract translation: 显示微生物生物膜的形成和维持依赖于由环状二-GMP介导的信号通路。 在没有这种信号传导的情况下，微生物与生物膜分离，从而变得更容易用常规抗生素治疗。 化学或生物意味着干扰环状二GMP信号传导诱导生物膜溶解，提供一类新型抗生素。 在本发明的一个实施方案中，生物膜抑制剂是循环二-GMP的类似物，其竞争性地或非竞争性地阻断信号传导。 在本发明的另一个实施方案中，生物膜抑制剂是干扰环状二-GMP合成或信号传导的遗传序列。