公开(公告)号：WO2006045041A3

公开(公告)日：2007-04-26

申请号：PCT/US2005037880

申请日：2005-10-18

Applicant: UNIV LELAND STANFORD JUNIOR ,  SPORMANN ALFRED M ,  THORMANN KAI M ,  SAVILLE RENEE M

Inventor： SPORMANN ALFRED M ,  THORMANN KAI M ,  SAVILLE RENEE M

IPC: C12Q1/18 ,  A61K31/7064 ,  A61K31/7068 ,  A61K31/7072 ,  A61K31/7076 ,  A61K31/708 ,  A61K31/7105 ,  A61K31/711

CPC classification number: C12Q1/18

Abstract: The formation and maintenance of microbial biofilms is shown to be dependent on signaling pathways mediated by cyclic di-GMP. In the absence of such signaling, microbes detach from a biofilm, and thereby become more readily treatable with conventional antibiotics. Chemical or biological means that interfere with cyclic-di-GMP signaling induce biofilm dissolution, providing for a new class of antibiotics. In one embodiment of the invention, the biofilm inhibitor is an analog of cyclic-di-GMP, which competitively or non-competitively blocks signaling. In another embodiment of the invention, the biofilm inhibitor is a genetic sequence that interferes with cyclic-di-GMP synthesis or signaling.

Abstract translation: 显示微生物生物膜的形成和维持依赖于由环状二-GMP介导的信号通路。 在没有这种信号传导的情况下，微生物与生物膜分离，从而变得更容易用常规抗生素治疗。 化学或生物意味着干扰环状二GMP信号传导诱导生物膜溶解，提供一类新型抗生素。 在本发明的一个实施方案中，生物膜抑制剂是循环二-GMP的类似物，其竞争性地或非竞争性地阻断信号传导。 在本发明的另一个实施方案中，生物膜抑制剂是干扰环状二-GMP合成或信号传导的遗传序列。

公开(公告)号：WO2006017576A3

公开(公告)日：2006-06-01

申请号：PCT/US2005027565

申请日：2005-08-02

Applicant: UNIV LELAND STANFORD JUNIOR ,  SPORMANN ALFRED M ,  MULLER JOCHEN A ,  ROSNER BETTINA M ,  VON ABENDROTH GREGORY ,  MESHULAM-SIMON GALIT ,  MCCARTY PERRY L

Inventor： SPORMANN ALFRED M ,  MULLER JOCHEN A ,  ROSNER BETTINA M ,  VON ABENDROTH GREGORY ,  MESHULAM-SIMON GALIT ,  MCCARTY PERRY L

IPC: C12N1/12 ,  A01N63/00

CPC classification number: C12N1/30 ,  B09C1/002 ,  C12N9/0004

Abstract: Compositions and methods are provided that relate to the bioremediation of chlorinated ethenes, particularly the bioremediation of vinyl chloride by Dehalococcoides-like organisms. An isolated strain of bacteria, Dehalococcoides sp. strain VS, that metabolizes vinyl chloride is provided; the genetic sequence of the enzyme responsible for vinyl chloride dehalogenation; methods of assessing the capability of endogenous organisms at an environmental site to metabolize vinyl chloride; and a method of using the strains of the invention for bioremediation.

Abstract translation: 提供了涉及氯化乙烯的生物修复，特别是类Dehalococcoides生物体对氯乙烯的生物修复的组合物和方法。 一种分离的细菌菌株Dehalococcoides sp。 提供代谢氯乙烯的菌株VS; 负责氯乙烯脱卤作用的酶的基因序列; 评估环境部位内源性有机体代谢氯乙烯能力的方法; 以及使用本发明的菌株进行生物修复的方法。

公开(公告)号：WO2006045041A2

公开(公告)日：2006-04-27

申请号：PCT/US2005/037880

申请日：2005-10-18

Applicant: THE BOARD OF TRUSTEES OF THE LELAND STANFORD JUNIOR UNIVERSITY ,  SPORMANN, Alfred, M. ,  THORMANN, Kai, M. ,  SAVILLE, Renee, M.

Inventor： SPORMANN, Alfred, M. ,  THORMANN, Kai, M. ,  SAVILLE, Renee, M.

IPC: C12M3/06

CPC classification number: C12Q1/18

Abstract: The formation and maintenance of microbial biofilms is shown to be dependent on signaling pathways mediated by cyclic di-GMP. In the absence of such signaling, microbes detach from a biofilm, and thereby become more readily treatable with conventional antibiotics. Chemical or biological means that interfere with cyclic-di-GMP signaling induce biofilm dissolution, providing for a new class of antibiotics. In one embodiment of the invention, the biofilm inhibitor is an analog of cyclic-di-GMP, which competitively or non-competitively blocks signaling. In another embodiment of the invention, the biofilm inhibitor is a genetic sequence that interferes with cyclic-di-GMP synthesis or signaling.

Abstract translation: 微生物生物膜的形成和维持显示依赖于由环状二-GMP介导的信号传导途径。 在没有这种信号的情况下，微生物与生物膜分离，从而变得更容易用常规抗生素治疗。 化学或生物意味着干扰环状二GMP信号传导诱导生物膜溶解，提供一类新型抗生素。 在本发明的一个实施方案中，生物膜抑制剂是循环二-GMP的类似物，其竞争性或非竞争性阻断信号传导。 在本发明的另一个实施方案中，生物膜抑制剂是干扰环状二-GMP合成或信号传导的遗传序列。

公开(公告)号：WO2006017576A2

公开(公告)日：2006-02-16

申请号：PCT/US2005/027565

申请日：2005-08-02

Applicant: THE BOARD OF TRUSTEES OF THE LELAND STANFORD JUNIOR UNIVERSITY ,  SPORMANN, Alfred M. ,  MULLER, Jochen A. ,  ROSNER, Bettina M. ,  VON ABENDROTH, Gregory ,  MESHULAM-SIMON, Galit ,  MCCARTY, Perry L.

Inventor： SPORMANN, Alfred M. ,  MULLER, Jochen A. ,  ROSNER, Bettina M. ,  VON ABENDROTH, Gregory ,  MESHULAM-SIMON, Galit ,  MCCARTY, Perry L.

IPC: C12N1/21

CPC classification number: C12N1/30 ,  B09C1/002 ,  C12N9/0004

Abstract: Compositions and methods are provided that relate to the bioremediation of chlorinated ethenes, particularly the bioremediation of vinyl chloride by Dehalococcoides -like organisms. An isolated strain of bacteria, Dehalococcoides sp. strain VS, that metabolizes vinyl chloride is provided; the genetic sequence of the enzyme responsible for vinyl chloride dehalogenation; methods of assessing the capability of endogenous organisms at an environmental site to metabolize vinyl chloride; and a method of using the strains of the invention for bioremediation.

Abstract translation: 提供了组合物和方法，其涉及氯代乙炔的生物修复，特别是由脱卤胆酸类生物体对氯乙烯的生物修复。 分离菌株，Dehalococcoides sp。 提供代谢氯乙烯的菌株VS; 负责氯乙烯脱卤的酶的基因序列; 评估环境部位内源生物体代谢氯乙烯能力的方法; 以及使用本发明的菌株进行生物修复的方法。