公开(公告)号：WO9813478A3

公开(公告)日：1998-08-20

申请号：PCT/EP9704923

申请日：1997-09-04

Applicant: MOGEN INT ,  STUIVER MAARTEN HENDRIK ,  CUSTERS JEROME HUBERTUS HENRIC ,  SELA BUURLAGE MARIANNE BEATRIX ,  MELCHERS LEO SJOERD ,  DEVENTER TROOST JOHANNA PIETER ,  LAGEWEG WESSEL ,  PONSTEIN ANNE SILENE

Inventor： STUIVER MAARTEN HENDRIK ,  CUSTERS JEROME HUBERTUS HENRIC ,  SELA-BUURLAGE MARIANNE BEATRIX ,  MELCHERS LEO SJOERD ,  VAN DEVENTER-TROOST JOHANNA PI ,  LAGEWEG WESSEL ,  PONSTEIN ANNE SILENE

IPC: A01H5/00 ,  C07K14/415 ,  C07K16/40 ,  C12N5/10 ,  C12N9/00 ,  C12N9/02 ,  C12N9/04 ,  C12N15/09 ,  C12N15/62 ,  C12N15/82 ,  C12Q1/68

CPC classification number: C12N9/0004 ,  A01N65/12 ,  C07K14/415 ,  C12N9/0006 ,  C12N15/8282

Abstract: The present invention provides an isolated protein obtainable from a plant source which has antifungal activity, specifically anti-Phytophthora activity and/or anti-Pythium activity and a molecular weight of about 55-65 kDa as judged by SDS PAGE-electrophoresis, an isolated DNA sequence comprising an open reading frame capable of encoding a protein according to the invention, preferably characterised in that it comprises an open reading frame which is capable of encoding a protein depicted in SEQ ID NO. 16, SEQ ID NO. 57, SEQ ID NO. 70, SEQ ID NO. 72 or SEQ ID NO. 74 or muteins thereof, and DNA capable of hybridising therewith under stringent conditions. The invention further comprises plants incorporating chimeric DNA capable of encoding a protein according to the invention, and wherein the protein is expressed. Also shown is the carbohydrate and preferably hexose oxidating activity of said protein. Also methods are provided for combating fungi, especially Phytophthora and Pythium species, using a protein or a host cell capable of producing the protein.

公开(公告)号：WO1998013478A2

公开(公告)日：1998-04-02

申请号：PCT/EP1997004923

申请日：1997-09-04

Applicant: MOGEN INTERNATIONAL N.V. ,  STUIVER, Maarten, Hendrik ,  CUSTERS, Jerôme, Hubertus, Henricus, Victor ,  SELA-BUURLAGE, Marianne, Beatrix ,  MELCHERS, Leo, Sjoerd ,  VAN DEVENTER-TROOST, Johanna, Pieternella, Els ,  LAGEWEG, Wessel ,  PONSTEIN, Anne, Silene

Inventor： MOGEN INTERNATIONAL N.V.

IPC: C12N09/00

CPC classification number: C12N9/0004 ,  A01N65/12 ,  C07K14/415 ,  C12N9/0006 ,  C12N15/8282

Abstract: The present invention provides an isolated protein obtainable from a plant source which has antifungal activity, specifically anti-Phytophthora activity and/or anti-Pythium activity and a molecular weight of about 55-65 kDa as judged by SDS PAGE-electrophoresis, an isolated DNA sequence comprising an open reading frame capable of encoding a protein according to the invention, preferably characterised in that it comprises an open reading frame which is capable of encoding a protein depicted in SEQ ID NO. 16, SEQ ID NO. 57, SEQ ID NO. 70, SEQ ID NO. 72 or SEQ ID NO. 74 or muteins thereof, and DNA capable of hybridising therewith under stringent conditions. The invention further comprises plants incorporating chimeric DNA capable of encoding a protein according to the invention, and wherein the protein is expressed. Also shown is the carbohydrate and preferably hexose oxidating activity of said protein. Also methods are provided for combating fungi, especially Phytophthora and Pythium species, using a protein or a host cell capable of producing the protein.

Abstract translation: 本发明提供从植物来源获得的分离的蛋白质，其具有抗真菌活性，特别是抗疫苗活性和/或抗 - 腐霉活性，并且通过SDS PAGE-电泳判断为约55-65kDa的分子量，分离的DNA 其包含能够编码本发明的蛋白质的开放阅读框，其特征在于，其包含能够编码SEQ ID NO：1所示蛋白质的开放阅读框。 16，SEQ ID NO： 57，SEQ ID NO： 70，SEQ ID NO： 72或SEQ ID NO。 74或突变蛋白，以及能够在严格条件下与其杂交的DNA。 本发明还包括含有能够编码本发明的蛋白质的嵌合DNA的植物，并且其中所述蛋白质被表达。 还显示了所述蛋白质的碳水化合物和优选己糖氧化活性。 还提供了使用能够产生蛋白质的蛋白质或宿主细胞来对抗真菌，特别是疫霉属（Phytophthora）和腐霉属（Pythium）物种的方法。

公开(公告)号：WO1995021929A1

公开(公告)日：1995-08-17

申请号：PCT/EP1995000488

申请日：1995-02-09

Applicant: MOGEN INTERNATIONAL N.V. ,  MELCHERS, Leo, Sjoerd ,  PONSTEIN, Anne, Silene ,  KROON-SWART, Saskia ,  VAN DEVENTER-TROOST, Johanna, Pieternella, Els ,  OHL, Stephan, Andreas ,  BRES-VLOEMANS, Alexandra, Aleida ,  LOGEMANN, Jürgen ,  SELA-BUURLAGE, Marianne, Beatrix

Inventor： MOGEN INTERNATIONAL N.V.

IPC: C12N15/82

CPC classification number: A01N65/38 ,  A01N65/00 ,  A01N65/385 ,  C07K14/415 ,  C12N15/8282 ,  A01N2300/00

Abstract: The present invention provides an isolated protein obtainable from a plant source which has anti-Phytophthora activity and a molecular weight of about 60 +/- 5 kDa as judged by SDS PAGE-electrophoresis, an isolated DNA sequence comprising an open reading frame capable of encoding a protein according to the invention, preferably characterized in that it comprises an open reading frame which is capable of encoding a protein as represented by amino acids 1 to 540 of SEQ ID NO: 6, or the precursor of said protein, and DNA capable of hybridising therewith under stringent conditions. The invention further comprises plants incorporating chimeric DNA capable of encoding a protein according to the invention, and wherein the protein is expressed. Also methods are provided for combatting fungi, especially Phytophthora infestans, using a protein or a host cell capable of producing the protein.

Abstract translation: 本发明提供了可从植物来源获得的分离蛋白质，其通过SDS PAGE-电泳判断具有抗疫病毒活性和约60 +/- 5kDa的分子量，分离的DNA序列包含能够编码的开放阅读框 根据本发明的蛋白质，其特征在于其包含能够编码由SEQ ID NO：6的氨基酸1至540表示的蛋白质或所述蛋白质的前体的蛋白质的开放阅读框，以及能够 在严格条件下与其杂交。 本发明还包括含有能够编码本发明的蛋白质的嵌合DNA的植物，并且其中所述蛋白质被表达。 还提供了使用能够产生蛋白质的蛋白质或宿主细胞来对抗真菌，特别是致病疫霉的方法。

公开(公告)号：WO1995005467A3

公开(公告)日：1995-02-23

申请号：PCT/EP1994002761

申请日：1994-08-17

Applicant: MOGEN INTERNATIONAL N.V. ,  RIJKSUNIVERSITEIT TE LEIDEN ,  APOTHEKER-DE GROOT, Marion ,  BOL, John, Ferdinand ,  CORNELISSEN, Bernardus, Johannes, Clemens ,  LINTHORST, Hubertus, Josephus, Maria ,  MELCHERS, Leo, Sjoerd ,  PONSTEIN, Anne, Silene ,  SELA-BUURLAGE, Marianne, Beatrix

Inventor： MOGEN INTERNATIONAL N.V. ,  RIJKSUNIVERSITEIT TE LEIDEN

IPC: C12N15/56

公开(公告)号：WO1994008009A1

公开(公告)日：1994-04-14

申请号：PCT/EP1993002790

申请日：1993-10-05

Applicant: MOGEN INTERNATIONAL N.V. ,  MELCHERS, Leo, Sjoerd ,  SELA-BUURLAGE, Marianne, Beatrix ,  BRES-VLOEMANS, Alexandra, Aleida ,  PONSTEIN, Anne, Silene ,  APOTHEKER, Marion ,  CORNELISSEN, Bernardus, Johannes, Clemens

Inventor： MOGEN INTERNATIONAL N.V.

IPC: C12N15/29

CPC classification number: C07K14/415 ,  C12N9/244 ,  C12N9/2442 ,  C12N15/8282 ,  C12Y302/01006 ,  C12Y302/01014

Abstract: A new category of antifungal chitin binding proteins (antifungal CBPs) is provided with very low chitinase activity (10 % or less than that of the class-I chitinases from tobacco). Also substantially pure DNA sequences encoding antifungal CBP and plant expressible antifungal CBP genes are provided for the obtention of transgenic plants producing antifungal CBP. Plants expressing an antifungal CBP gene, optionally in combination with a plant expressible glucanase gene, show reduced susceptibility to fungi.

Abstract translation: 提供了一种新类型的抗真菌几丁质结合蛋白（抗真菌CBPs）具有非常低的几丁质酶活性（10％或更少于来自烟草的I类几丁质酶的活性）。 还提供了编码抗真菌CBP和植物可表达的抗真菌CBP基因的基本上纯的DNA序列用于获得产生抗真菌CBP的转基因植物。 表达抗真菌CBP基因的植物，任选地与植物可表达的葡聚糖酶基因组合显示降低的对真菌的敏感性。

公开(公告)号：WO9505467A2

公开(公告)日：1995-02-23

申请号：PCT/EP9402761

申请日：1994-08-17

Applicant: MOGEN INT ,  UNIV LEIDEN ,  GROOT MARION DE ,  BOL JOHN FERDINAND ,  CORNELISSEN BERNARDUS JOHANNES ,  LINTHORST HUBERTUS J M ,  MELCHERS LEO SJOERD ,  PONSTEIN ANNE SILENE ,  SELA BUURLAGE MARIANNE BEATRIX

Inventor： APOTHEKER-DE GROOT MARION ,  BOL JOHN FERDINAND ,  CORNELISSEN BERNARDUS JOHANNES ,  LINTHORST HUBERTUS JOSEPHUS MA ,  MELCHERS LEO SJOERD ,  PONSTEIN ANNE SILENE ,  SELA-BUURLAGE MARIANNE BEATRIX

IPC: A01H5/00 ,  A01N65/00 ,  A01N65/36 ,  A01N65/38 ,  A01P3/00 ,  A61P31/10 ,  C07K14/415 ,  C12N1/21 ,  C12N5/10 ,  C12N9/24 ,  C12N15/09 ,  C12N15/56 ,  C12N15/82 ,  C12R1/01

CPC classification number: A01N65/385 ,  A01N65/00 ,  A01N65/38 ,  C12N9/2442 ,  C12N15/8282 ,  C12Y302/01014 ,  A01N2300/00

Abstract: The present invention provides a novel class of plant chitinases, which have endo-chitinase activity, antifungal activity and a molecular weight of about 40 to 43 kDa as judged by Sodium Dodecyl Sulphate Polyacrylamide electrophoreses. The invention further provides antifungal compositions comprising as active ingredient a chitinase according to the invention. According to a different aspect of the invention an isolated DNA sequence is provided which comprises an open reading frame encoding a novel chitinase. The invention also encompasses a plant expressible recombinant DNA sequence which comprises a DNA sequence encoding a chitinase according to the invention, as well as plant cells and plants which have such recombinant DNA incorporated into their genome.

Abstract translation: 本发明提供了一类新颖的植物几丁质酶，其具有内切几丁质酶活性，抗真菌活性和约40至43kDa的分子量，由十二烷基硫酸钠聚丙烯酰胺电泳鉴定。 本发明进一步提供了包含根据本发明的几丁质酶作为活性成分的抗真菌组合物。 根据本发明的不同方面，提供了分离的DNA序列，其包含编码新几丁质酶的开放阅读框。 本发明还包括植物可表达的重组DNA序列，其包含编码根据本发明的几丁质酶的DNA序列，以及将这种重组DNA并入其基因组中的植物细胞和植物。

公开(公告)号：WO1991018984A2

公开(公告)日：1991-12-12

申请号：PCT/NL1991000089

申请日：1991-05-31

Applicant: MOGEN INTERNATIONAL N.V. ,  WOLOSHUK, Charles, Peter ,  MELCHERS, Leo, Sjoerd ,  CORNELISSEN, Bernardus, Johannes, Clemens ,  MEULENHOFF, Elisabeth, Josine, Sophie ,  SELA-BUURLAGE, Marianne, Beatrix ,  VAN DEN ELZEN, Petrus, Josephus, Maria

Inventor： MOGEN INTERNATIONAL N.V.

IPC: C12N15/29

CPC classification number: A01N65/20 ,  A01N65/00 ,  A01N65/08 ,  A01N65/10 ,  A01N65/38 ,  A61K38/00 ,  C07K14/415 ,  C12N15/8282 ,  A01N2300/00

Abstract: New methods are provided for the identification and purification of proteins from plants with an inhibitory effect on pathogens and/or pests, such as insects, nematodes, fungi, and bacteria, using a combination of non-denaturing protein fractionation techniques on leaf-extracts of plants which were treated to develop induced resistance and an antipathogenic assay. Also provided are antifungal proteins obtained by the said method, recombinant nucleotide sequences encoding the said proteins, as well as plants with reduced susceptibility to fungi due to production and desired targeting of the said proteins in the plant.