公开(公告)号：WO9505467A2

公开(公告)日：1995-02-23

申请号：PCT/EP9402761

申请日：1994-08-17

Applicant: MOGEN INT ,  UNIV LEIDEN ,  GROOT MARION DE ,  BOL JOHN FERDINAND ,  CORNELISSEN BERNARDUS JOHANNES ,  LINTHORST HUBERTUS J M ,  MELCHERS LEO SJOERD ,  PONSTEIN ANNE SILENE ,  SELA BUURLAGE MARIANNE BEATRIX

Inventor： APOTHEKER-DE GROOT MARION ,  BOL JOHN FERDINAND ,  CORNELISSEN BERNARDUS JOHANNES ,  LINTHORST HUBERTUS JOSEPHUS MA ,  MELCHERS LEO SJOERD ,  PONSTEIN ANNE SILENE ,  SELA-BUURLAGE MARIANNE BEATRIX

IPC: A01H5/00 ,  A01N65/00 ,  A01N65/36 ,  A01N65/38 ,  A01P3/00 ,  A61P31/10 ,  C07K14/415 ,  C12N1/21 ,  C12N5/10 ,  C12N9/24 ,  C12N15/09 ,  C12N15/56 ,  C12N15/82 ,  C12R1/01

CPC classification number: A01N65/385 ,  A01N65/00 ,  A01N65/38 ,  C12N9/2442 ,  C12N15/8282 ,  C12Y302/01014 ,  A01N2300/00

Abstract: The present invention provides a novel class of plant chitinases, which have endo-chitinase activity, antifungal activity and a molecular weight of about 40 to 43 kDa as judged by Sodium Dodecyl Sulphate Polyacrylamide electrophoreses. The invention further provides antifungal compositions comprising as active ingredient a chitinase according to the invention. According to a different aspect of the invention an isolated DNA sequence is provided which comprises an open reading frame encoding a novel chitinase. The invention also encompasses a plant expressible recombinant DNA sequence which comprises a DNA sequence encoding a chitinase according to the invention, as well as plant cells and plants which have such recombinant DNA incorporated into their genome.

Abstract translation: 本发明提供了一类新颖的植物几丁质酶，其具有内切几丁质酶活性，抗真菌活性和约40至43kDa的分子量，由十二烷基硫酸钠聚丙烯酰胺电泳鉴定。 本发明进一步提供了包含根据本发明的几丁质酶作为活性成分的抗真菌组合物。 根据本发明的不同方面，提供了分离的DNA序列，其包含编码新几丁质酶的开放阅读框。 本发明还包括植物可表达的重组DNA序列，其包含编码根据本发明的几丁质酶的DNA序列，以及将这种重组DNA并入其基因组中的植物细胞和植物。

公开(公告)号：WO9950423A3

公开(公告)日：1999-12-16

申请号：PCT/EP9902176

申请日：1999-03-25

Applicant: MOGEN INT ,  UNIV LEIDEN ,  UNIV NIJMEGEN ,  LINTHORST HUBERTUS J M ,  VERPOORTE ROBERT ,  VERBERNE MARIA CATHARINA ,  MORENO PAULO R H ,  TEGELEN LEONARDUS JOHANNES PET ,  WULLEMS GEORGE JOSEPH ,  CROES ANTON FELIX ,  STUIVER MAARTEN HENDRIK ,  CUSTERS JEROME HUBERTINA HENRI ,  SIMONS LAMBERTUS HENRICUS ,  MELCHERS LEO SJOERD ,  BOL JOHN FERDINAND

Inventor： LINTHORST HUBERTUS JOSEPHUS MA ,  VERPOORTE ROBERT ,  VERBERNE MARIA CATHARINA ,  MORENO PAULO R H ,  VAN TEGELEN LEONARDUS JOHANNES ,  WULLEMS GEORGE JOSEPH ,  CROES ANTON FELIX ,  STUIVER MAARTEN HENDRIK ,  CUSTERS JEROME HUBERTINA HENRI ,  SIMONS LAMBERTUS HENRICUS ,  MELCHERS LEO SJOERD ,  BOL JOHN FERDINAND

IPC: A01H5/00 ,  C12N1/21 ,  C12N5/10 ,  C12N9/88 ,  C12N9/90 ,  C12N15/09 ,  C12N15/61 ,  C12N15/82 ,  C12N15/84

CPC classification number: C12N9/90 ,  C12N9/88 ,  C12N15/8239 ,  C12N15/8282 ,  C12N15/8283

Abstract: This invention describes a method to induce pathogen resistance in plants, characterized in that plants are transformed with an expression cassette harboring a gene coding for an isochorismate synthase. More specifically, this method is characterized in that the gene coding for isochorismate synthase is selected from a group consisting of entC, orfA, pchA and ICS, this last gene preferably the ICS gene from Catharantus roseus. Another embodiment of the invention is a method according to the method described above, characterized in that plants are additionally transformed with an expression cassette harboring a gene coding for an isochorismate pyruvate lyase, preferably on the same expression cassette as the gene coding for isochorismate synthase. The gene coding for isochorismate pyruvate lyase is preferably selected from the group consisting of orfD and pchB. A further aspect of the invention is a protein having isochorismate synthase activity which is isolated from Catharantus roseus. Still a further aspect of the invention is the nucleotide sequence comprising the 5' regulatory region which is naturally found to regulate the expression of the ICS gene in Catharantus roseus.

Abstract translation: 本发明描述了一种在植物中诱导病原体抗性的方法，其特征在于用含有等位基因合成酶基因的表达盒转化植物。 更具体地说，该方法的特征在于编码等分子合成酶的基因选自由entC，orfA，pchA和ICS组成的组，最后一个基因优选来自玫瑰花（Catharantus roseus）的ICS基因。 本发明的另一个实施方案是根据上述方法的方法，其特征在于用含有编码异激酶丙酮酸裂合酶的基因的表达盒另外转化植物，优选与编码等位基因合成酶的基因位于相同的表达盒上。 编码异激酶丙酮酸裂合酶的基因优选选自orfD和pchB。 本发明的另一方面是具有等鞭毛状合成酶活性的蛋白质，其从玫瑰红蔷薇中分离。 本发明的另一方面是包含天然存在的调节Cusarantus roseus中ICS基因表达的5'调节区的核苷酸序列。