中科微点-全球专利检索

  1. Login
  2. Sign Up

Search Results

16 records (took 0.035 seconds)

    AUTOMATED SAMPLE-TO-MICROARRAY SYSTEM
    3.
    发明申请
    AUTOMATED SAMPLE-TO-MICROARRAY SYSTEM 审中-公开
    自动样本到微型系统

    公开(公告)号：WO2007142692A3

    公开(公告)日：2009-05-07

    申请号：PCT/US2006060867

    申请日：2006-11-14

    Applicant: US GOV SEC NAVY ,  LIGLER FRANCES S ,  STENGER DAVID A ,  ERICKSON JEFF ,  ARCHER MARIE

    Inventor： LIGLER FRANCES S ,  STENGER DAVID A ,  ERICKSON JEFF ,  ARCHER MARIE

    IPC: C12Q1/68 ,  C07H21/04 ,  C12P19/34

    CPC classification number: B01L3/5027 ,  B01L7/52 ,  B01L2200/028 ,  B01L2200/04 ,  B01L2200/0631 ,  B01L2200/10 ,  B01L2200/16 ,  B01L2300/0636 ,  B01L2300/0681 ,  B01L2300/0816 ,  B01L2300/1827 ,  B01L2400/0421 ,  B01L2400/0439 ,  B01L2400/0487 ,  C12Q2565/629 ,  G01N2035/00158

    Abstract: An apparatus having within or as part of a housing; a sample port; a microarray port; a lysis module; a purification module for containing a solid phase for binding of oligonucleotides; a thermocycling module for containing a polymerase chain reaction; a fragmentation module; and a microarray module for holding a microarray and a liquid in contact with the microarray. The apparatus is configured to be coupled to a device for: pumping a liquid through, in order, the lysis, purification, thermocycling, fragmentation, and microarray modules; sonicating any contents of the lysis module; thermocycling the thermocycling module to perform the polymerase chain reaction; heating the fragmentation module to fragment any oligonucleotides contained therein; circulating a fluid over the surface of the microarray; and performing one or more washing or staining steps on the microarray.

    Abstract translation: 具有在壳体内或作为壳​​体的一部分的装置; 样品端口 一个微阵列港口 裂解模块; 用于含有寡核苷酸结合的固相的纯化模块; 用于含有聚合酶链式反应的热循环模块; 碎片模块; 以及用于保持微阵列和与微阵列接触的液体的微阵列模块。 该装置被配置为耦合到装置，用于：按顺序泵送液体裂解，纯化，热循环，碎裂和微阵列模块; 超声处理裂解模块的任何内容物; 热循环模块进行聚合酶链反应; 加热片段化模块以使其中所含的任何寡核苷酸片段化; 使流体在微阵列的表面上循环; 并在微阵列上进行一个或多个洗涤或染色步骤。

    COMPUTER-IMPLEMENTED BIOLOGICAL SEQUENCE IDENTIFIER SYSTEM AND METHOD
    4.
    发明申请
    COMPUTER-IMPLEMENTED BIOLOGICAL SEQUENCE IDENTIFIER SYSTEM AND METHOD 审中-公开
    计算机实现生物学序列识别系统和方法

    公开(公告)号：WO2006138182A2

    公开(公告)日：2006-12-28

    申请号：PCT/US2006/022622

    申请日：2006-06-09

    Applicant: THE GOVERN. OF THE UNITED STATES OF AMERICA, AS REPRESENTED BY THE SECRETARY OF THE NAVY ,  MALANOSKI, Anthony, P. ,  LIN, Baochuan ,  SCHNUR, Joel, M. ,  STENGER, David, A.

    Inventor： MALANOSKI, Anthony, P. ,  LIN, Baochuan ,  SCHNUR, Joel, M. ,  STENGER, David, A.

    IPC: C12Q1/70 ,  C12Q1/68 ,  C12M1/34

    CPC classification number: G06F19/22 ,  G06F19/18 ,  G06F19/20 ,  Y10S707/99936

    Abstract: A method of: submitting reference sequences to a taxonomic database to produce taxonomic results; and reporting a taxonomic identification based on the taxonomic results. The reference sequences are the output of genetic database queries that return a score for each reference sequence. A method for processing a biological sequence obtained from an assay by: converting base calls located in a predetermined list of positions within the biological sequence to N; and determining the ratio of single nucleotide polymorphisms in the biological sequence relative to a reference sequence. Each entry in the predetermined list of positions represents the capability of a substance hybridizing to a microarray used to generate the biological sequence. The substance is not the nucleic acid of a target pathogen.

    Abstract translation: 一种方法：将参考序列提交给分类数据库以产生分类结果; 并根据分类结果报告分类鉴定。 参考序列是返回每个参考序列得分的遗传数据库查询的输出。 一种用于处理通过以下方式获得的生物学序列的方法：将位于所述生物学序列内的预定位置列表中的碱基呼叫转化为N; 并确定生物序列中单核苷酸多态性相对于参考序列的比例。 预定位置列表中的每个条目表示与用于产生生物序列的微阵列杂交的物质的能力。 该物质不是目标病原体的核酸。

    MULTIPLEXED POLYMERASE CHAIN REACTION FOR GENETIC SEQUENCE ANALYSIS
    6.
    发明申请
    MULTIPLEXED POLYMERASE CHAIN REACTION FOR GENETIC SEQUENCE ANALYSIS 审中-公开
    用于遗传序列分析的多重聚合酶链反应

    公开(公告)号：WO2006138183A3

    公开(公告)日：2007-05-31

    申请号：PCT/US2006022623

    申请日：2006-06-09

    Applicant: US GOV SEC NAVY ,  LIN BAOCHUAN ,  BLANEY KATE M ,  MALANOSKI ANTHONY P ,  SCHNUR JOEL M ,  STENGER DAVID A

    Inventor： LIN BAOCHUAN ,  BLANEY KATE M ,  MALANOSKI ANTHONY P ,  SCHNUR JOEL M ,  STENGER DAVID A

    IPC: C12Q1/68 ,  C12P19/34

    CPC classification number: C12Q1/689 ,  C12Q2600/16

    Abstract: A PCR method involving: providing a biological sample suspected of containing one or more pathogen nucleic acids; adding a plurality of PCR primers corresponding to genes found in the pathogens; and performing a polymerase chain reaction on the sample to amplify a subset of the nucleic acids that correspond to the genes. The primers include at least one primer pair for each pathogen, and the primers contain a tail sequence that is not homologous any pathogen DNA or to any background DNA in the sample. The concentration of at least one primer in the polymerase chain reaction is no more than about 100 nM.

    Abstract translation: 一种PCR方法，包括：提供怀疑含有一种或多种病原体核酸的生物样品; 添加对应于病原体中发现的基因的多个PCR引物; 并对样品进行聚合酶链反应以扩增与该基因相对应的核酸的子集。 引物包括每个病原体的至少一个引物对，并且引物含有与样品中任何病原体DNA或任何背景DNA不同源的尾部序列。 聚合酶链反应中至少一个引物的浓度不超过约100nM。

    SOLID PHASE FOR CAPTURE OF NUCLEIC ACIDS
    8.
    发明申请
    SOLID PHASE FOR CAPTURE OF NUCLEIC ACIDS 审中-公开
    固体捕获核酸的相

    公开(公告)号：WO2009015002A3

    公开(公告)日：2009-03-26

    申请号：PCT/US2008070419

    申请日：2008-07-18

    Applicant: US GOV SEC NAVY ,  ARCHER MARIE J ,  LIN BAOCHUAN ,  STENGER DAVID A

    Inventor： ARCHER MARIE J ,  LIN BAOCHUAN ,  STENGER DAVID A

    IPC: C40B50/18

    CPC classification number: C12N15/1006 ,  C07B2200/11 ,  C12N15/1013 ,  C12Q1/68 ,  C40B30/04 ,  C40B40/08 ,  C40B50/18

    Abstract: A method of: providing a solid surface having a dendrimer molecule bound thereto and a single-stranded probe nucleic acid immobilized to the dendrimer; contacting the solid surface with a sample suspected or known to contain a double-stranded complimentary target nucleic acid; denaturing the target nucleic acids at thermal conditions and in a salt concentration sufficient to denature the target nucleic acids to produce denatured nucleic acids; and cooling the sample to allow hybridization of the denatured nucleic acids to the probe nucleic acids. An article having: one or more paramagnetic microbeads; a dendrimer molecule bound to the beads; and a probe nucleic acid immobilized to the dendrimer.

    Abstract translation: 一种方法：提供具有结合到其上的树枝状大分子分子的固体表面和固定在树状聚合物上的单链探针核酸; 使所述固体表面与疑似或已知含有双链互补靶核酸的样品接触; 在热条件下和在足以使目标核酸变性以产生变性核酸的盐浓度下使目标核酸变性; 并冷却样品以允许变性核酸与探针核酸杂交。 一种具有：一个或多个顺磁性微珠的物品; 与珠结合的树枝状大分子; 和固定在树状聚合物上的探针核酸。

    SOLID PHASE FOR CAPTURE OF NUCLEIC ACIDS
    9.
    发明申请
    SOLID PHASE FOR CAPTURE OF NUCLEIC ACIDS 审中-公开
    用于捕获核酸的固相

    公开(公告)号：WO2009015002A2

    公开(公告)日：2009-01-29

    申请号：PCT/US2008/070419

    申请日：2008-07-18

    Applicant: THE GOVERNMENT OF THE UNITED STATES OF AMERICA, as represented by THE SECRETARY OF THE NAVY ,  ARCHER, Marie, J. ,  LIN, Baochuan ,  STENGER, David, A

    Inventor： ARCHER, Marie, J. ,  LIN, Baochuan ,  STENGER, David, A

    IPC: C40B50/18 ,  C40B40/08

    CPC classification number: C12N15/1006 ,  C07B2200/11 ,  C12N15/1013 ,  C12Q1/68 ,  C40B30/04 ,  C40B40/08 ,  C40B50/18

    Abstract: A method of: providing a solid surface having a dendrimer molecule bound thereto and a single-stranded probe nucleic acid immobilized to the dendrimer; contacting the solid surface with a sample suspected or known to contain a double-stranded complimentary target nucleic acid; denaturing the target nucleic acids at thermal conditions and in a salt concentration sufficient to denature the target nucleic acids to produce denatured nucleic acids; and cooling the sample to allow hybridization of the denatured nucleic acids to the probe nucleic acids. An article having: one or more paramagnetic microbeads; a dendrimer molecule bound to the beads; and a probe nucleic acid immobilized to the dendrimer.

    Abstract translation: 提供具有与其结合的树枝状聚合物分子和固定在树枝状聚合物上的单链探针核酸的固体表面的方法; 使固体表面与怀疑或已知含有双链互补靶核酸的样品接触; 在热条件和足以使靶核酸变性以产生变性核酸的盐浓度下变性靶核酸; 并冷却样品以允许变性的核酸与探针核酸杂交。 一种物品，具有：一个或多个顺磁性微珠; 与珠粒结合的树枝状聚合物分子; 和固定在树状聚合物上的探针核酸。

    DESIGN AND SELECTION OF GENETIC TARGETS FOR SEQUENCE RESOLVED ORGANISM DETECTION AND IDENTIFICATION
    10.
    发明申请
    DESIGN AND SELECTION OF GENETIC TARGETS FOR SEQUENCE RESOLVED ORGANISM DETECTION AND IDENTIFICATION 审中-公开
    遗传靶向序列分析有机体检测和鉴定的设计和选择

    公开(公告)号：WO2008024827A3

    公开(公告)日：2008-12-11

    申请号：PCT/US2007076499

    申请日：2007-08-22

    Applicant: US GOV SEC NAVY ,  MALANOSKI ANTHONY P ,  WANG ZHENG ,  LIN BAOCHUAN ,  STENGER DAVID A ,  SCHNUR JOEL M

    Inventor： MALANOSKI ANTHONY P ,  WANG ZHENG ,  LIN BAOCHUAN ,  STENGER DAVID A ,  SCHNUR JOEL M

    IPC: G01N33/48 ,  C12Q1/68

    CPC classification number: G06F19/22 ,  C12Q1/6834 ,  C12Q1/701 ,  C12Q2600/112 ,  C12Q2600/158 ,  G06F19/20

    Abstract: A computer-implemented method as follows. Providing a list of target sequences associated with one or more organisms in a list of organisms. Providing a list of candidate prototype sequences suspected of hybridizing to one or more of the target sequences. Generating a collection of probes corresponding to each candidate prototype sequence, each collection of probes having a set of probes for every subsequence having a predetermined, fixed subsequence length of the corresponding candidate prototype sequence. The sets consist of the corresponding subsequence and every variation of the corresponding subsequence formed by varying a center nucleotide of the corresponding subsequence. Generating a set of fragments corresponding to each target sequence, each set of fragments having every fragment having a predetermined, fixed fragment length of the corresponding target sequence. Calculating the binding free energy of each fragment with a perfect complimentary sequence of the fragment. If any binding free energy is above a predetermined, fixed threshold, the fragment is extended one nucleotide at a time until the binding free energy is below the threshold or the fragment is the same length as the probe, generating a set of extended fragments. Determining which extended fragments are perfect matches to any of the probes. Assembling a base call sequence corresponding to each candidate prototype sequence. The base call sequence has a base call corresponding to the center nucleotide of each probe of the corresponding prototype sequence that is a perfect match to any extended fragment, but for which the other members of the set of probes containing the perfect match probe are not perfect matches to any extended fragment and a non-base call in all other circumstances.

    Abstract translation: 计算机实现的方法如下。 提供与生物体列表中的一个或多个生物体相关联的目标序列的列表。 提供怀疑与一个或多个靶序列杂交的候选原型序列的列表。 生成对应于每个候选原型序列的探针集合，每个探针集合具有针对每个子序列具有相应候选原型序列的预定的固定子序列长度的一组探针。 该组包括相应的子序列和通过改变相应子序列的中心核苷酸形成的相应子序列的每个变化。 生成对应于每个靶序列的一组片段，每组片段具有每个片段具有相应靶序列的预定的固定片段长度。 用完美的片段互补序列计算每个片段的结合自由能。 如果任何结合自由能高于预定的固定阈值，则该片段一次延伸一个核苷酸直到结合自由能低于阈值或该片段与探针长度相同，产生一组扩增片段。 确定哪些扩展片段与任何探针完美匹配。 组装与每个候选原型序列对应的基本调用序列。 碱基调用序列具有对应于相应原型序列的每个探针的中心核苷酸的碱基调用，该碱基调用与任何扩展片段完美匹配，但是包含完美匹配探针的该组探针的其他成员不完美 在所有其他情况下匹配任何扩展片段和非基础调用。

Patent Agency Ranking