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公开(公告)号：WO2004069865A3
公开(公告)日：2005-02-10
申请号：PCT/NL2004000083
申请日：2004-02-06
Applicant: UNIV LEIDEN , STICHTING BINAIR VECTOR SYSTEE , HOOYKAAS PAUL JAN JACOB , VAN DER ZAAL ENGELBERTUS JACOB
IPC: C07K14/415 , C12N15/29 , C12N15/82
CPC classification number: C12N15/8287 , C07K14/415 , C12N15/8262
Abstract: The invention a method to modulate plant growth or developmental processes in a plant or plant cell, more in particular organogenesis and/or embryogenesis, through the provision of a plant protein which can act a transcriptional regulator of said processes. The invention further provides a plant and propagating material thereof which contains in its genome nucleotide sequence encoding a protein of the invention, said protein characterised in that it comprises the amino acid sequence PRGRPPGSKNK.
Abstract translation: 本发明通过提供可以作用于所述方法的转录调节剂的植物蛋白质来调节植物或植物细胞中的植物生长或发育过程，更特别是器官发生和/或胚胎发生的方法。 本发明还提供了一种植物及其繁殖材料，其在其基因组中含有编码本发明蛋白质的核苷酸序列，所述蛋白质的特征在于其包含氨基酸序列PRGRPPGSKNK。
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公开(公告)号：WO2004069865A8
公开(公告)日：2005-03-31
申请号：PCT/NL2004000083
申请日：2004-02-06
Applicant: UNIV LEIDEN , STICHTING BINAIR VECTOR SYSTEE , HOOYKAAS PAUL JAN JACOB , VAN DER ZAAL ENGELBERTUS JACOB
IPC: C07K14/415 , C12N15/29 , C12N15/82
CPC classification number: C12N15/8287 , C07K14/415 , C12N15/8262
Abstract: The invention a method to modulate plant growth or developmental processes in a plant or plant cell, more in particular organogenesis and/or embryogenesis, through the provision of a plant protein which can act a transcriptional regulator of said processes. The invention further provides a plant and propagating material thereof which contains in its genome nucleotide sequence encoding a protein of the invention, said protein characterised in that it comprises the amino acid sequence PRGRPPGSKNK.
Abstract translation: 本发明通过提供可作用所述过程的转录调节因子的植物蛋白来调节植物或植物细胞中的植物生长或发育过程，更具体而言是器官发生和/或胚胎发生的方法。 本发明进一步提供了在其基因组中含有编码本发明蛋白质的核苷酸序列的植物和繁殖材料，所述蛋白质的特征在于它包含氨基酸序列PRGRPPGSKNK。
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公开(公告)号：CA2409236A1
公开(公告)日：2001-11-29
申请号：CA2409236
申请日：2001-05-21
Applicant: STICHTING TECH WETENSCHAPP , UNIV LEIDEN , STICHTING BINAIR VECTOR SYSTEE
Abstract: A method of effecting a change in a cell using a transfer system which is contacted with the cell to be changed. The transfer system comprises a fusion protein which is transferred into the cell using the VirB/VirD4 transfer system. According to the invention, a fusion protein BA is introduced into the cell to be changed comprising i) as a first part A, an oligopeptide comprising the C-terminal amino acids 1-20 of VirF, VirD2, VirE2, VirE3, VirD5 or MobA, or an analogue thereof and ii) as a second part B, a polypeptide capable of effecting a cell-changing activity in the cell to be changed, wherein the polypeptide is attached with the C-terminal end thereof linked with the N-terminal end of the first part.
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公开(公告)号：WO02052026A8
公开(公告)日：2003-11-27
申请号：PCT/NL0100936
申请日：2001-12-21
Applicant: UNIV LEIDEN , STICHTING BINAIR VECTOR SYSTEE , HOOYKAAS PAUL JAN JACOB , VAN ATTIKUM HAICO , BUNDOCK PAUL
Inventor： HOOYKAAS PAUL JAN JACOB , VAN ATTIKUM HAICO , BUNDOCK PAUL
CPC classification number: C12N15/8213 , C12N15/81 , C12N15/902 , C12N15/905
Abstract: The invention relate to the field of molecular biology and cell biology. It particularly relates to methods to direct integration of a nucleic acid of interest towards homologous recombination and uses thereof. The present invention discloses factors involved in integration of a nucleic acid by illegitimate recombination which provides a method to direct integration of a nucleic acid of interest to a pre-determined site, whereby said nucleic acid has homology at or around the said pre-determined site, in a eukaryote with a preference for non-homologous recombination comprising steering an integration pathway towards homologous recombination. Furthermore, the invention provides a method to direct integration of a nucleic acid of interest to a sub-telomeric and/or telomeric region in a eukaryote with a preference for non-homologous recombination.
Abstract translation: 本发明涉及分子生物学和细胞生物学领域。 它特别涉及将感兴趣的核酸直接整合到同源重组及其用途的方法。 本发明公开了涉及通过非法重组整合核酸的因素，其提供了将感兴趣的核酸直接整合到预定位点的方法，由此所述核酸在所述预定位点处或周围具有同源性 ，在优先选择非同源重组的真核生物中，包括转向同源重组的整合途径。 此外，本发明提供了一种直接将目的核酸整合到真核生物中的亚端粒和/或端粒区域并优先选择非同源重组的方法。
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Patent Agency Ranking
公开(公告)号：WO02052026A3
公开(公告)日：2002-09-06
申请号：PCT/NL0100936
申请日：2001-12-21
Applicant: UNIV LEIDEN , STICHTING BINAIR VECTOR SYSTEE , HOOYKAAS PAUL JAN JACOB , VAN ATTIKUM HAICO , BUNDOCK PAUL
Inventor： HOOYKAAS PAUL JAN JACOB , VAN ATTIKUM HAICO , BUNDOCK PAUL
CPC classification number: C12N15/8213 , C12N15/81 , C12N15/902 , C12N15/905
Abstract: The invention relate to the field of molecular biology and cell biology. It particularly relates to methods to direct integration of a nucleic acid of interest towards homologous recombination and uses thereof. The present invention discloses factors involved in integration of a nucleic acid by illegitimate recombination which provides a method to direct integration of a nucleic acid of interest to a pre-determined site, whereby said nucleic acid has homology at or around the said pre-determined site, in a eukaryote with a preference for non-homologous recombination comprising steering an integration pathway towards homologous recombination. Furthermore, the invention provides a method to direct integration of a nucleic acid of interest to a sub-telomeric and/or telomeric region in a eukaryote with a preference for non-homologous recombination.
Abstract translation: 本发明涉及分子生物学和细胞生物学领域。 它特别涉及将感兴趣的核酸直接整合到同源重组及其用途的方法。 本发明公开了涉及通过非法重组整合核酸的因素，其提供了将感兴趣的核酸直接整合到预定位点的方法，由此所述核酸在所述预定位点处或周围具有同源性 ，在优先选择非同源重组的真核生物中，包括转向同源重组的整合途径。 此外，本发明提供了一种直接将目的核酸整合到真核生物中的亚端粒和/或端粒区域并优先选择非同源重组的方法。
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公开(公告)号：CA2515333A1
公开(公告)日：2004-08-19
申请号：CA2515333
申请日：2004-02-06
Applicant: UNIV LEIDEN , STICHTING BINAIR VECTOR SYSTEE
IPC: C07K14/415 , C12N15/29 , C12N15/82
Abstract: The invention a method to modulate plant growth or developmental processes in a plant or plant cell, more in particular organogenesis and/or embryogenesis, through the provision of a plant protein which can act a transcriptional regulator of said processes. The invention further provides a plant and propagating material thereof which contains in its genome nucleotide sequence encoding a protein of the invention, said protein characterised in that it comprises the amino acid sequence PRGRPXGSKNK, wherein X is A, P, L or R.
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公开(公告)号：CA2432717A1
公开(公告)日：2002-07-04
申请号：CA2432717
申请日：2001-12-21
Applicant: UNIV LEIDEN , STICHTING BINAIR VECTOR SYSTEE
Inventor： VAN ATTIKUM HAICO , HOOYKAAS PAUL JAN JACOB , BUNDOCK PAUL
Abstract: The invention relate to the field of molecular biology and cell biology. It particularly relates to methods to direct integration of a nucleic acid of interest towards homologous recombination and uses thereof. The present invention discloses factors involved in integration of a nucleic acid by illegitimate recombination which provides a method to direct integration of a nucleic acid of interest to a pre-determined site, whereby said nucleic acid has homology at or around the said pre-determined site, in a eukaryote with a preference for non-homologous recombination comprising steering an integration pathway towards homologous recombination. Furthermore, the invention provides a method to direct integration of a nucleic acid of interest to a sub- telomeric and/or telomeric region in a eukaryote with a preference for non- homologous recombination.
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公开(公告)号：AU4127101A
公开(公告)日：2001-09-12
申请号：AU4127101
申请日：2001-03-01
Applicant: UNIV LEIDEN , STICHTING BINAIR VECTOR SYSTEE
Inventor： HOOYKAAS PAUL JAN JACOB , ESCUDERO JESUS
Abstract: The invention relates to the transfer of genetic material to eukaryotic cells by means of a process resembling conjugation, in particular by a system partially based on Agrobacterium tumefaciens-like transfer systems. In particular transfer of genetic material into plant cells is disclosed using mobilisable, but non-conjugative plasmids by means of an Agrobacterium virulence system. The invention provides a method for transferring genetic material which is not a typical T-DNA surrounded by Agrobacterium T-borders from an Agrobacterium virulence system to a eukaryotic host cell, comprising providing said genetic material on a mobilisable plasmid, capable of forming a relaxosome, bringing said mobilisable plasmid in an Agrobacterium having at least the activity of the transfer genes of Agrobacterium not present on said mobilisable plasmid, whereby the necessary gene products providing the same or similar activity as a functional VirB operon are also present and cocultivating said agrobacterium with said eukaryotic host cell.
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公开(公告)号：CA2401909A1
公开(公告)日：2001-09-07
申请号：CA2401909
申请日：2001-03-01
Applicant: UNIV LEIDEN , STICHTING BINAIR VECTOR SYSTEE
Inventor： HOOYKAAS PAUL JAN JACOB , ESCUDERO JESUS
Abstract: The invention relates to the transfer of genetic material to eukaryotic cells by means of a process resembling conjugation, in particular by a system partially based on Agrobacterium tumefaciens-like transfer systems. In particular transfer of genetic material into plant cells is disclosed using mobilisable, but non-conjugative plasmids by means of an Agrobacterium virulence system. The invention provides a method for transferring genetic material which is not a typical T-DNA surrounded by Agrobacterium T-borders from an Agrobacterium virulence system to a eukaryotic host cell, comprising providing said genetic material on a mobilisable plasmid, capable of forming a relaxosome, bringing said mobilisable plasmid in an Agrobacterium having at least the activity of the transfer genes of Agrobacterium not present on said mobilisable plasmid, whereby the necessary gene products providing the same or similar activity as a functional VirB operon are also present and cocultivating said agrobacterium with said eukaryotic host cell.
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