公开(公告)号：CA2409236C

公开(公告)日：2014-03-04

申请号：CA2409236

申请日：2001-05-21

Applicant: STICHTING TECH WETENSCHAPP ,  UNIV LEIDEN ,  STICHTING BINAIR VECTOR SYSTEM

Inventor： HOOYKAAS PAUL JAN JACOB ,  VERGUNST ANNETTE CAROLINE ,  SCHRAMMEIJER BARBARA

IPC: C12N15/62 ,  C07K14/195 ,  C07K19/00 ,  C12N5/10 ,  C12N9/00 ,  C12N15/63 ,  C12N15/82

Abstract: The invention relates to a method of effecting a change in a cell using a transfer system which is contacted with the cell to be changed. The transfer system comprises a fusion protein which is transferred into the cell using the VirB/VirD4 transfer system. According to the invention a fusion protein BA is introduced into the cell to be changed comprising i) as a first part A an oligopeptide comprising 3 adjacent amino acids identical to or corresponding to the amino acids of the C-termninal amino acids 1-40 of VirF, VirD2, VirE2, VirE3 or MobA, and ii) as a second part B a polypeptide capable of effecting a cell-changing activity in the cell to be changed, wherein the polypeptide is attached with the C-terminal end thereof with the N-terminal end of the first part.

公开(公告)号：AU6438901A

公开(公告)日：2001-12-03

申请号：AU6438901

申请日：2001-05-21

Applicant: STICHTING TECH WETENSCHAPP ,  UNIV LEIDEN ,  STICHTING BINAIR VECTOR SYSTEM

Inventor： HOOYKAAS PAUL JAN JACOB ,  VERGUNST ANNETTE CAROLINE ,  SCHRAMMEIJER BARBARA

IPC: C07K14/195 ,  C12N9/00 ,  C12N15/62 ,  C12N15/82

Abstract: A method of effecting a change in a cell using a transfer system which is contacted with the cell to be changed. The transfer system comprises a fusion protein which is transferred into the cell using the VirB/VirD4 transfer system. According to the invention, a fusion protein BA is introduced into the cell to be changed comprising i) as a first part A, an oligopeptide comprising the C-terminal amino acids 1-20 of VirF, VirD2, VirE2, VirE3, VirD5 or MobA, or an analogue thereof and ii) as a second part B, a polypeptide capable of effecting a cell-changing activity in the cell to be changed, wherein the polypeptide is attached with the C-terminal end thereof linked with the N-terminal end of the first part.

公开(公告)号：NL1015252C2

公开(公告)日：2001-11-20

申请号：NL1015252

申请日：2000-05-19

Applicant: STICHTING TECH WETENSCHAPP ,  UNIV LEIDEN ,  STICHTING BINAIR VECTOR SYSTEM

Inventor： HOOYKAAS PAUL JAN JACOB ,  VERGUNST ANNETTE CAROLINE ,  SCHRAMMEIJER BARABARA

IPC: C07K14/195 ,  C12N9/00 ,  C12N15/62 ,  C12N15/82

Abstract: A method of effecting a change in a cell using a transfer system which is contacted with the cell to be changed. The transfer system comprises a fusion protein which is transferred into the cell using the VirB/VirD4 transfer system. According to the invention, a fusion protein BA is introduced into the cell to be changed comprising i) as a first part A, an oligopeptide comprising the C-terminal amino acids 1-20 of VirF, VirD2, VirE2, VirE3, VirD5 or MobA, or an analogue thereof and ii) as a second part B, a polypeptide capable of effecting a cell-changing activity in the cell to be changed, wherein the polypeptide is attached with the C-terminal end thereof linked with the N-terminal end of the first part.