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    Magnetocaloric effect of Mn-Fe-P-Si-B-V alloy and use thereof
    3.
    发明专利
    Magnetocaloric effect of Mn-Fe-P-Si-B-V alloy and use thereof 未知

    公开(公告)号：NL2022331B1

    公开(公告)日：2020-08-19

    申请号：NL2022331

    申请日：2018-12-31

    Applicant: UNIV DELFT TECH ,  STICHTING TECHNISCHE WETENSCHAPPEN

    Inventor： EKKEHARD HUBERTUS BRÜCK ,  JAIWEI LAI ,  NIELS HARMEN VAN DIJK

    IPC: H01F1/01 ,  H01F41/00

    Abstract: The invention provides an alloy comprising eg. manganese, iron, vanadium, phosphor and silicon. The invention also provides an apparatus comprising a magnetic field generator, a heat sink, the thermo element, a heat source, and a control system, wherein in a controlling mode the control system is configured to select between (i) a first configuration wherein the magnetic field generator generates a magnetic field, the thermo element is eXposed to the magnetic field, and heat from the thermo element is transferred to the heat sink, and (ii) a second configuration, wherein the thermo element is not exposed to the magnetic field, and heat from a heat source is transferred to the thermo element.

    Thermostable Cas9 nucleases
    4.
    发明专利
    Thermostable Cas9 nucleases 未知

    公开(公告)号：AU2017377136A1

    公开(公告)日：2019-07-11

    申请号：AU2017377136

    申请日：2017-08-16

    Applicant: STICHTING TECHNISCHE WETENSCHAPPEN ,  UNIV WAGENINGEN

    Inventor： VAN DER OOST JOHN ,  VAN KRANENBURG RICHARD ,  BOSMA ELLEKE FENNA ,  MOUGIAKOS IOANNIS ,  MOHANRAJU PRARTHANA

    IPC: C12N9/22 ,  C12N15/113

    Abstract: The present invention relates to the field of genetic engineering and more particularly to nucleic acid editing and genome modification. The present invention provides an isolated Cas protein or polypeptide fragment thereof having an amino acid sequence of SEQ ID NO: 1 or a sequence of at least 77% identity therewith. The Cas protein or polypeptide is capable of binding, cleaving, marking or modifying a double stranded target polynucleotide at a temperature in the range 20°C and 100°C inclusive. The invention further provides isolated nucleic acid molecules encoding said Cas9 nucleases, expression vectors and host cells. The invention also provides PAM sequences recognized by the Cas protein or polypeptide, The Cas9 nucleases disclosed herein provide novel tools for genetic engineering in general, in particular at elevated temperatures.

    Thermostable Cas9 nucleases
    5.
    发明专利
    Thermostable Cas9 nucleases 未知

    公开(公告)号：AU2017377135A1

    公开(公告)日：2019-07-11

    申请号：AU2017377135

    申请日：2017-08-16

    Applicant: STICHTING TECHNISCHE WETENSCHAPPEN ,  UNIV WAGENINGEN

    Inventor： VAN DER OOST JOHN ,  VAN KRANENBURG RICHARD ,  BOSMA ELLEKE FENNA ,  MOUGIAKOS IOANNIS ,  MOHANRAJU PRARTHANA

    IPC: C12N9/22 ,  C12N15/113

    Abstract: Thermostable Cas9 nucleases. The present invention relates to the field of genetic engineering and more particularly to nucleic acid editing and genome modification. The present invention provides an isolated Cas protein or polypeptide fragment thereof having an amino acid sequence of SEQ ID NO: 1 or a sequence of at least 77% identity therewith. The Cas protein or polypeptide is capable of binding, cleaving, marking or modifying a double stranded target polynucleotide at a temperature in the range 20°C and 100°C inclusive. The invention further provides isolated nucleic acid molecules encoding said Cas9 nucleases, expression vectors and host cells. The invention also provides PAM sequences recognized by the Cas protein or polypeptide. The Cas9 nucleases disclosed herein provide novel tools for genetic engineering in general, in particular at elevated temperatures and are of particular value in the genetic manipulation of thermophilic organisms; particularly microorganisms.

    THERMOSTABLE CAS9 NUCLEASES
    6.
    发明专利
    THERMOSTABLE CAS9 NUCLEASES 未知

    公开(公告)号：CA3046842A1

    公开(公告)日：2018-06-21

    申请号：CA3046842

    申请日：2017-12-14

    Applicant: UNIV WAGENINGEN ,  STICHTING TECHNISCHE WETENSCHAPPEN

    Inventor： VAN DER OOST JOHN ,  VAN KRANENBURG RICHARD ,  BOSMA ELLEKE FENNA ,  MOUGIAKOS IOANNIS ,  MOHANRAJU PRARTHANA

    IPC: C12N9/22 ,  C12N15/113 ,  C12N15/90

    Abstract: A polynucleotide encoding a ThermoCas9protein from Geobacillus thermodenitrificans and a constitutive promoter are used to engineer eukaryotic cells, e.g. fungi, yeast or algae, so that the ThermoCas9 endonuclease is integrated and expressed from the genome of the cell. Then, a second expression plasmid is used to transfect these ThermoCas9 expressing cells, the second plasmid containing an inducible promoter and a polynucleotide encoding a guide RNA. The guide RNA combines with the ThermoCas9 to provide the targeted endonuclease activity to cleave the cell DNA at a desired locus or gene of interest. A repair-oligo is also provided to the cell whereby following DNA cleavage, homologous recombination takes place in the cell with the repair-oligo so that either a deletion or substitution of nucleotides in the locus or gene of interest is achieved. Expression vectors and methods of using the vectors to achieve ThermoCas9 mediated gene editing are described whereby higher temperatures, e.g. greater than 30 °C, are used.

    THERMOSTABLE CAS9 NUCLEASES
    7.
    发明专利
    THERMOSTABLE CAS9 NUCLEASES 未知

    公开(公告)号：CA3046828A1

    公开(公告)日：2018-06-21

    申请号：CA3046828

    申请日：2017-08-16

    Applicant: UNIV WAGENINGEN ,  STICHTING TECHNISCHE WETENSCHAPPEN

    Inventor： VAN DER OOST JOHN ,  VAN KRANENBURG RICHARD ,  BOSMA ELLEKE FENNA ,  MOUGIAKOS IOANNIS ,  MOHANRAJU PRARTHANA

    IPC: C12N9/22 ,  C12N15/113

    Abstract: The present invention relates to the field of genetic engineering and more particularly to nucleic acid editing and genome modification. The present invention provides an isolated Cas protein or polypeptide fragment thereof having an amino acid sequence of SEQ ID NO: 1 or a sequence of at least 77% identity therewith. The Cas protein or polypeptide is capable of binding, cleaving, marking or modifying a double stranded target polynucleotide at a temperature in the range 20°C and 100°C inclusive. The invention further provides isolated nucleic acid molecules encoding said Cas9 nucleases, expression vectors and host cells. The invention also provides PAM sequences recognized by the Cas protein or polypeptide, The Cas9 nucleases disclosed herein provide novel tools for genetic engineering in general, in particular at elevated temperatures.

    THERMOSTABLE CAS9 NUCLEASES
    8.
    发明专利
    THERMOSTABLE CAS9 NUCLEASES 未知

    公开(公告)号：CA3046824A1

    公开(公告)日：2018-06-21

    申请号：CA3046824

    申请日：2016-12-14

    Applicant: UNIV WAGENINGEN ,  STICHTING TECHNISCHE WETENSCHAPPEN

    Inventor： VAN DER OOST JOHN ,  VAN KRANENBURG RICHARD ,  BOSMA ELLEKE FENNA ,  MOUGIAKOS IOANNIS

    IPC: C12N9/22 ,  C12N15/113

    Abstract: Thermostable Cas9 nucleases. The present invention relates to the field of genetic engineering and more particularly to nucleic acid editing and genome modification. The present invention provides an isolated Cas protein or polypeptide fragment thereof having an amino acid sequence of SEQ ID NO: 1 or a sequence of at least 77% identity therewith. The Cas protein or polypeptide is capable of binding, cleaving, marking or modifying a double stranded target polynucleotide at a temperature in the range 30°C and 100°C inclusive. The invention further provides isolated nucleic acid molecules encoding said Cas9 nucleases, expression vectors and host cells. The invention also provides PAM sequences recognized by the Cas protein or polypeptide, The Cas9 nucleases disclosed herein provide novel tools for genetic engineering at elevated temperatures and are of particular value in the genetic manipulation of thermophilic organisms; particularly microorganisms.

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