公开(公告)号：US20240209046A1

公开(公告)日：2024-06-27

申请号：US18453221

申请日：2023-08-21

Applicant: Korea Institute of Ceramic Engineering and Technology ,  ToolBio. Co

Inventor： Sung Hyun Kim ,  Jin Ho Bang ,  Mi Hyun Choi ,  Seho Park

IPC: C07K14/47 ,  C07K1/30 ,  C07K14/475 ,  C07K14/485 ,  C07K14/50 ,  C07K14/51 ,  C07K14/605 ,  C07K14/745 ,  C07K14/78 ,  C12N9/08 ,  C12N9/50 ,  C12N15/70 ,  C12P21/02

CPC classification number: C07K14/4728 ,  C07K1/303 ,  C07K14/475 ,  C07K14/485 ,  C07K14/50 ,  C07K14/51 ,  C07K14/605 ,  C07K14/745 ,  C07K14/78 ,  C12N9/0065 ,  C12N9/506 ,  C12N15/70 ,  C12P21/02 ,  C12Y111/01007 ,  C12Y304/22044 ,  C07K2319/20

Abstract: The present disclosure relates to a method for expressing, water-solubilizing, and purifying protein by using a calsequestrin-tag (CSQ-tag). Also provided are: a recombinant expression vector containing a CSQ-tag and a target protein; a host cell transformed using the recombinant expression vector; and a method for expressing and purifying a target protein by using a CSQ tag.