公开(公告)号：WO2010088245A1

公开(公告)日：2010-08-05

申请号：PCT/US2010/022188

申请日：2010-01-27

Applicant: BIO-RAD LABORATORIES, INC. ,  WALDBESER, David, E. ,  BHATT, Neeraj ,  MCDONALD, Kevin, A.

Inventor： WALDBESER, David, E. ,  BHATT, Neeraj ,  MCDONALD, Kevin, A.

IPC: H05B35/00

CPC classification number: G01N27/44721 ,  G01N27/44726 ,  G01N27/44782

Abstract: An adaptor is designed as an accessory to an ultraviolet transilluminator for the excitation of fluorescent molecules or labels in a planar array of biochemical samples such as a two-dimensional electrophoresis gel to enable the emissions resulting from the excitation to be detected and quantified. The adaptor is constructed to overlay the transilluminator and contains both a fluorescent dye that upon excitation by ultraviolet light emits light in the visible spectrum, and a conditioning substance that selects a portion of the wavelength band of the visible light produced by the fluorescent dye. The adaptor converts the ultraviolet light from the transilluminator to visible light while limiting the emissions reaching the detector to those that emanate from the sample. By the use of this adaptor, the transilluminator is adapted for use with samples labeled with dyes that are excitable by visible light and avoids exposure of the samples and the user to ultraviolet light.

Abstract translation: 适配器被设计为紫外透射仪的附件，用于激发生物化学样品（例如二维电泳凝胶）的平面阵列中的荧光分子或标记，以使得能够检测和量化激发产生的排放。 适配器被构造成覆盖透照器并且包含在紫外光激发时发射可见光谱中的光的荧光染料和选择由荧光染料产生的可见光的一部分波长带的调理物质。 适配器将来自透照射器的紫外光转换成可见光，同时将到达检测器的排放物限制为从样品发出的排放物。 通过使用该适配器，透射仪适用于可用可见光激发的染料标记的样品，并避免样品和使用者暴露于紫外线。

公开(公告)号：WO2008033625A3

公开(公告)日：2008-06-26

申请号：PCT/US2007075329

申请日：2007-08-07

Applicant: BIO RAD LABORATORIES ,  HILLENDAHL JAMES W ,  WALDBESER DAVID E

Inventor： HILLENDAHL JAMES W ,  WALDBESER DAVID E

IPC: G02B6/32 ,  G01N1/10 ,  G01N21/25 ,  G01N21/64

CPC classification number: G01N21/6452 ,  G01N2021/6421 ,  G01N2021/6471 ,  G01N2021/6484

Abstract: Biochemical assays that utilize excitation and light emission as labels for detection are enhanced by an illumination and detection system that supplies excitation light through an optical fiber that transmits excitation light from an excitation light source to the sample Emission light produced by the excitation is then collected by a lens and converted to a signal that is compiled by conventional softwar for analysis The optical fiber transfixes (passes through) the lens via a slot or other opening and is preferably offset from the center of the lens The optical fiber and the light collecting lens are arranged such that the direction of travel of the excitation light and the direction along which the emission light is collected are not coaxial, and preferably both are at an acute angle to the axis normal to the mouth of the receptacle.

Abstract translation: 利用激发和发光作为检测标签的生物化学测定通过照射和检测系统得到增强，所述照明和检测系统通过将激发光从激发光源传播到样品的光纤提供激发光，然后由激发产生的发射光由 透镜并转换为通过常规软件编译的信号用于分析光纤经由狭槽或其他开口经过透镜（穿过）透镜，并且优选地偏离透镜的中心。光纤和聚光透镜是 被布置为使得激发光的行进方向和发射光被收集的方向不同轴，并且优选地两者与垂直于容器的嘴的轴成锐角。

公开(公告)号：WO2008033625A2

公开(公告)日：2008-03-20

申请号：PCT/US2007/075329

申请日：2007-08-07

Applicant: BIO-RAD LABORATORIES, INC. ,  HILLENDAHL, James W. ,  WALDBESER, David E.

Inventor： HILLENDAHL, James W. ,  WALDBESER, David E.

IPC: G02B6/32

CPC classification number: G01N21/6452 ,  G01N2021/6421 ,  G01N2021/6471 ,  G01N2021/6484

Abstract: Biochemical assays of samples in receptacles, in gels, in blots, in arrays and the like, and that utilize excitation and light emission as labels for detection are enhanced by an illumination and detection system that supplies excitation light through an optical fiber that transmits excitation light from an excitation light source to the sample. Emission light produced by the excitation is then collected by a lens and converted to a signal that is compiled by conventional software for analysis. The optical fiber transfixes (passes through) the lens via a slot or other opening and is preferably offset from the center of the lens. The optical fiber and collecting lens can either be on the same side of the sample or on opposite sides, i.e., one above and the other below. The optical fiber and the light collecting lens are arranged such that the direction of travel of the excitation light and the direction along which the emission light is collected are not coaxial, and preferably both are at an acute angle to the axis normal to the mouth of the receptacle. Illumination systems are also disclosed in which a ultraviolet, visible, or near-infrared light source is optically coupled to an optical fiber.

Abstract translation: 通过照射和检测系统增强了在容器，凝胶，印迹，阵列等中的样品和利用激发和发光作为检测标签的样品的生物化学分析，其通过透射激发光的光纤提供激发光 从激发光源到样品。 由激发产生的发射光然后由透镜收集并转换成由常规软件编译的信号用于分析。 光纤经由狭槽或其他开口将透镜经过（透过）透镜，并且优选地偏离透镜的中心。 光纤和收集透镜可以在样品的相同侧或者在相对的两侧，即一个在另一个之上。 光纤和聚光透镜被布置成使得激发光的行进方向和发射光被收集的方向不同轴，并且优选地两者都与垂直于嘴的轴成锐角 容器 还公开了照明系统，其中紫外线，可见光或近红外光源光学耦合到光纤。