公开(公告)号：WO1997030166A1

公开(公告)日：1997-08-21

申请号：PCT/NL1997000066

申请日：1997-02-14

Applicant: CENTRUM VOOR PLANTENVEREDELINGS- EN REPRODUKTIEONDERZOEK (CPRO-DLO) ,  KATHOLIEKE UNIVERSITEIT NIJMEGEN ,  OLDENHOF, Margryt, Teatske ,  CUSTERS, Johannes, Bernardus, Maria ,  SCHRAUWEN, Jan, Antonius, Maria ,  VAN LOOKEREN CAMPAGNE, Michiel, Maurits ,  WULLEMS, George, Joseph ,  DONS, Johannes, Jacobus, Maria

Inventor： CENTRUM VOOR PLANTENVEREDELINGS- EN REPRODUKTIEONDERZOEK (CPRO-DLO) ,  KATHOLIEKE UNIVERSITEIT NIJMEGEN

IPC: C12N15/82

CPC classification number: C12N15/8222 ,  C07K14/415 ,  C12N15/8231 ,  C12N15/8289

Abstract: The present invention is related to isolated purified DNA sequences from the promoter region of a microspore-specific gene of tobacco. Said sequences can act as microspore-specific promoters and can play a role in the expression of genes in microspores. The invention also relates to chimeric genes suitable for transforming plants comprising the present microspore-specific promoter. Further, the invention relates to plants transformed with said chimeric genes.

Abstract translation: 本发明涉及来自烟草小孢子特异性基因的启动子区域的分离的纯化DNA序列。 所述序列可以作为小孢子特异性启动子，并且可以在小孢子中表达基因中发挥作用。 本发明还涉及适于转化包含本发明的小孢子特异性启动子的植物的嵌合基因。 此外，本发明涉及用所述嵌合基因转化的植物。

公开(公告)号：WO9950423A3

公开(公告)日：1999-12-16

申请号：PCT/EP9902176

申请日：1999-03-25

Applicant: MOGEN INT ,  UNIV LEIDEN ,  UNIV NIJMEGEN ,  LINTHORST HUBERTUS J M ,  VERPOORTE ROBERT ,  VERBERNE MARIA CATHARINA ,  MORENO PAULO R H ,  TEGELEN LEONARDUS JOHANNES PET ,  WULLEMS GEORGE JOSEPH ,  CROES ANTON FELIX ,  STUIVER MAARTEN HENDRIK ,  CUSTERS JEROME HUBERTINA HENRI ,  SIMONS LAMBERTUS HENRICUS ,  MELCHERS LEO SJOERD ,  BOL JOHN FERDINAND

Inventor： LINTHORST HUBERTUS JOSEPHUS MA ,  VERPOORTE ROBERT ,  VERBERNE MARIA CATHARINA ,  MORENO PAULO R H ,  VAN TEGELEN LEONARDUS JOHANNES ,  WULLEMS GEORGE JOSEPH ,  CROES ANTON FELIX ,  STUIVER MAARTEN HENDRIK ,  CUSTERS JEROME HUBERTINA HENRI ,  SIMONS LAMBERTUS HENRICUS ,  MELCHERS LEO SJOERD ,  BOL JOHN FERDINAND

IPC: A01H5/00 ,  C12N1/21 ,  C12N5/10 ,  C12N9/88 ,  C12N9/90 ,  C12N15/09 ,  C12N15/61 ,  C12N15/82 ,  C12N15/84

CPC classification number: C12N9/90 ,  C12N9/88 ,  C12N15/8239 ,  C12N15/8282 ,  C12N15/8283

Abstract: This invention describes a method to induce pathogen resistance in plants, characterized in that plants are transformed with an expression cassette harboring a gene coding for an isochorismate synthase. More specifically, this method is characterized in that the gene coding for isochorismate synthase is selected from a group consisting of entC, orfA, pchA and ICS, this last gene preferably the ICS gene from Catharantus roseus. Another embodiment of the invention is a method according to the method described above, characterized in that plants are additionally transformed with an expression cassette harboring a gene coding for an isochorismate pyruvate lyase, preferably on the same expression cassette as the gene coding for isochorismate synthase. The gene coding for isochorismate pyruvate lyase is preferably selected from the group consisting of orfD and pchB. A further aspect of the invention is a protein having isochorismate synthase activity which is isolated from Catharantus roseus. Still a further aspect of the invention is the nucleotide sequence comprising the 5' regulatory region which is naturally found to regulate the expression of the ICS gene in Catharantus roseus.

Abstract translation: 本发明描述了一种在植物中诱导病原体抗性的方法，其特征在于用含有等位基因合成酶基因的表达盒转化植物。 更具体地说，该方法的特征在于编码等分子合成酶的基因选自由entC，orfA，pchA和ICS组成的组，最后一个基因优选来自玫瑰花（Catharantus roseus）的ICS基因。 本发明的另一个实施方案是根据上述方法的方法，其特征在于用含有编码异激酶丙酮酸裂合酶的基因的表达盒另外转化植物，优选与编码等位基因合成酶的基因位于相同的表达盒上。 编码异激酶丙酮酸裂合酶的基因优选选自orfD和pchB。 本发明的另一方面是具有等鞭毛状合成酶活性的蛋白质，其从玫瑰红蔷薇中分离。 本发明的另一方面是包含天然存在的调节Cusarantus roseus中ICS基因表达的5'调节区的核苷酸序列。