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公开(公告)号：KR1020020022286A
公开(公告)日：2002-03-27
申请号：KR1020000054999
申请日：2000-09-19
Applicant: (주)바이오니아
IPC: B01D39/16
CPC classification number: B01D39/1623 , B01D39/1615 , B01D39/2017 , B01D2239/0464 , B01D2239/065 , B01D2239/10 , C12N15/1017
Abstract: PURPOSE: A multi-layer filter for separating and purifying nucleic acid is provided in which a plurality of filter layers having different pore sizes are combined in a lamella structure so as to used in separating and purifying the nucleic acid from biological samples. CONSTITUTION: The multi-layer filter is characterized in that synthetic resin filter layers(2-9) having an average pore diameter of 5 to 40 microns are arranged as first to eighth layers on a hydrophobic filter layer(1) having an average pore diameter of 1 to 10 microns, wherein the hydrophobic filter layer(1) is a hydrophobic synthetic resin filter layer or a hydrophobic surface treated glass filter layer, the synthetic resin filter layer(1) is made of polyolefin resin such as polyethylene resin, the glass filter layer is covered with dimethyl dichloride silane, diethyl dichloride silane, or a mixture thereof, and the multi-layer filter further comprises a paper filter layer having a thickness of 1 to 2 mm and an average pore diameter of 5 to 25 microns which can be formed on the hydrophobic filter layer(1) or interposed between the synthetic resin filter layers(2-9), and which is manufactured by impregnating a paper filter with a buffer solution containing sodium acetate or potassium acetate and having a pH of 3 to 7, and drying the buffer solution impregnated paper filter.
Abstract translation: 目的：提供用于分离和纯化核酸的多层过滤器，其中具有不同孔径的多个过滤层以片层结构组合以便用于从生物样品中分离和纯化核酸。 构成：多层过滤器的特征在于平均孔径为5至40微米的合成树脂过滤层（2-9）被布置成具有平均孔径的疏水性过滤层（1）上的第一至第八层 为1〜10微米，其中疏水性过滤层（1）为疏水性合成树脂过滤层或疏水性表面处理玻璃过滤层，合成树脂过滤层（1）为聚乙烯树脂等聚烯烃树脂，玻璃 过滤层被二氯甲烷硅烷，二氯化二氯硅烷或其混合物覆盖，多层过滤器还包括厚度为1-2mm，平均孔径为5至25微米的纸过滤层，其可以 形成在疏水性过滤层（1）上或插入在合成树脂过滤层（2-9）之间，通过用含有乙酸钠或钾的缓冲溶液浸渍纸过滤器 乙酸盐，pH为3〜7，干燥缓冲溶液浸渍纸过滤器。
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公开(公告)号：KR1020010090338A
公开(公告)日：2001-10-18
申请号：KR1020000015205
申请日：2000-03-24
Applicant: (주)바이오니아
IPC: C12M1/00
Abstract: PURPOSE: A device for micro-arraying a biological sample automatically is provided to manufacture microchips, on which the bio-molecules are micro-arrayed, simply in large quantities by spotting the biological sample as the high density and micro-arraying it. CONSTITUTION: The device for micro-arraying the biological sample automatically includes a capillary tube, plates(2), a rack(10), a withdrawing hook(13), a fixed board(19), a vacuum pad(20) and a cover opening and shutting instrument(14). The capillary tube takes the biological sample in the plates(2) and drips it on the board. The plates(2) are laid up and down and on the direction of the circumference. The rack(10) is operated possible to be rotated and to be inserted and removed. The withdrawing hook(13) withdraws the plates(2) from the rack(10) in the horizontal direction. The vacuum pad(20) is installed on the fixed board(19) to fix the board. The cover opening and shutting instrument(14) opens the cover of the plates(2).
Abstract translation: 目的：提供一种用于自动微阵列生物样品的装置，用于制造微芯片，生物分子在其上进行微阵列，通过将生物样品点样作为高密度和微阵列，可以大量量产。 构成：用于微生物样品的微阵列的装置自动包括毛细管，板（2），齿条（10），抽出钩（13），固定板（19），真空垫（20）和 盖打开和关闭仪器（14）。 毛细管将生物样品置于板（2）中，并将其滴在板上。 板（2）在圆周方向上下放置。 机架（10）可以旋转并被插入和移除。 取出钩（13）在水平方向上从支架（10）中退出板（2）。 真空垫（20）安装在固定板（19）上以固定板。 盖打开和关闭仪器（14）打开板（2）的盖。
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公开(公告)号：KR1020010106068A
公开(公告)日：2001-11-29
申请号：KR1020000049805
申请日：2000-08-26
Applicant: (주)바이오니아
IPC: C12N1/20
Abstract: New sulfonyloxadiazolones have the formula (I), in which A stands for hydrogen, sulphur, -SO-, -SO 2 - or a group (a), in which R 3 stands for hydrogen or alkyl; R 1 stands for optionally substituted cycloalkyl, cycloalkenyl or aryl; and R 2 stands for optionally substituted heterocyclyl. Also disclosed is a process for preparing these substances and their use as microbicides for the protection of plants and materials.
Abstract translation: 新的磺酰氧基二唑类具有式（I），其中A代表氢，硫，-SO-，-SO 2 - 或基团（a），其中R 3代表氢或烷基; R 1表示任选取代的环烷基，环烯基或芳基; 且R 2表示任选取代的杂环基。 还公开了制备这些物质及其作为杀微生物剂用于保护植物和材料的用途的方法。
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Patent Agency Ranking
公开(公告)号：KR100404236B1
公开(公告)日：2003-11-01
申请号：KR1020000049805
申请日：2000-08-26
Applicant: (주)바이오니아
IPC: C12N1/20
Abstract: New sulfonyloxadiazolones have the formula (I), in which A stands for hydrogen, sulphur, -SO-, -SO 2 - or a group (a), in which R 3 stands for hydrogen or alkyl; R 1 stands for optionally substituted cycloalkyl, cycloalkenyl or aryl; and R 2 stands for optionally substituted heterocyclyl. Also disclosed is a process for preparing these substances and their use as microbicides for the protection of plants and materials.
Abstract translation: 新的磺酰基恶二唑酮具有式（I），其中A代表氢，硫，-SO-，-SO 2 - 或基团（a），其中R 3代表氢或烷基; R 1代表任选取代的环烷基，环烯基或芳基; 并且R 2代表任选取代的杂环基。 还公开了制备这些物质及其作为杀微生物剂用于保护植物和材料的用途的方法。
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公开(公告)号：KR100404235B1
公开(公告)日：2003-11-01
申请号：KR1020000054999
申请日：2000-09-19
Applicant: (주)바이오니아
IPC: B01D39/16
Abstract: PROBLEM TO BE SOLVED: To provide a multilayer filter for nucleic acid separation and refining, which has high efficiency, selectivity and strength. SOLUTION: This multilayer filter consists of a porous synthetic resin layer(s), a glass layer(s) and a paper layer(s) and such plural multilayer filters provided are generally classified into two kinds of multilayer filters, that is,: one kind of the multilayer filters are trapping filters each of which is capable of preventing any permeation of a whole cell-fragmented material solution containing nucleic acid from being caused when filtration is performed under ordinary pressure, and when filtration is performed under reduced pressure, allowing only a nucleic acid-containing solution to permeate through and trapping the cell residue; and the other kind of the multilayer filters are binding filters each of which is capable of adsorbing plasmid DNA in the presence of a chaotropic salt and desorbing the adsorbed plasmid DNA when an eluting buffer solution is added.
Abstract translation: 待解决的技术问题：提供一种高效，高选择性，高强度的核酸分离精制用多层过滤器。 该多层过滤器由多孔合成树脂层，玻璃层和纸层组成，并且所提供的这种多层过滤器通常分为两种多层过滤器，即：一个 多层过滤器的种类是各自能够防止在常压下过滤时引起含有核酸的全细胞片段化材料溶液的任何渗透，并且当在减压下进行过滤时，仅允许 含有核酸的溶液以渗透并捕获细胞残留物; 而另一种多层过滤器是结合过滤器，它们中的每一个都能够在离液序列高的盐存在下吸附质粒DNA，并且当加入洗脱缓冲溶液时能够解吸吸附的质粒DNA。
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公开(公告)号：KR1020030023812A
公开(公告)日：2003-03-20
申请号：KR1020010056919
申请日：2001-09-14
Applicant: (주)바이오니아
IPC: C07H1/06
Abstract: PURPOSE: A multi-layered filter for separation and purification of nucleic acid is provided, thereby cell cultivation, cell lysis and filtration processes can be continuously carried out in a vessel. CONSTITUTION: The multi-layered filter for separation and purification of nucleic acid comprises a first lowest filter layer having average pore diameter of 1 to 10 μm and 2nd to 8th synthetic filter layers having average pore diameter of 5 to 40 μm, wherein the average pore diameter of the filter layers is 1 to 5 μm; the filter layer is hydrophobic synthetic resin, hydrophobic-treated glass or paper filter layer; the synthetic filter layer is made of polyolefin resin; the polyolefin resin is polyethylene resin; a filter layer, which is dipped in a buffer solution of pH 3 to 7 containing sodium acetate or potassium acetate and has the average pore diameter of 5 to 25 μm, may be further added on the 8th synthetic filter layer.
Abstract translation: 目的：提供用于分离和纯化核酸的多层过滤器，从而可以在容器中连续进行细胞培养，细胞裂解和过滤过程。 构成：用于分离和纯化核酸的多层过滤器包括平均孔径为1至10μm的第一最低过滤层和平均孔径为5至40μm的第2至第8合成过滤层，其中平均孔 过滤层的直径为1〜5μm; 过滤层为疏水性合成树脂，疏水处理玻璃或纸过滤层; 合成过滤层由聚烯烃树脂制成; 聚烯烃树脂是聚乙烯树脂; 可以在第八合成过滤层上进一步添加浸渍在含有乙酸钠或乙酸钾的平均孔径为5〜25μm的pH3〜7的缓冲溶液中的过滤层。
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9.发明公开아밀라제의 활성을 억제할 수 있는 옥수수 추출물, 이를함유하는 비만증 또는 당뇨병 치료 또는 예방용 의약적조성물 및 식품 첨가물 그리고 그들의 제조방법 无效玉米提取物抑制活性的药物，药物组合物和食品添加剂，用于治疗和预防肥胖或糖尿病及其生产
公开(公告)号：KR1020030025200A
公开(公告)日：2003-03-28
申请号：KR1020020056818
申请日：2002-09-18
Applicant: (주)바이오니아
IPC: A61K36/899 , A61P3/04 , A61P3/10
CPC classification number: A61K36/899 , A23L33/105
Abstract: PURPOSE: A process of preparing a corn extract by extracting a corn kernel or corn husk in war, a polar organic solvent or a mixture thereof and then removing the extraction solvent from the extract is provided. Therefore, a composition containing the corn extract is effectively used as a pharmaceutical composition for the prophylaxis and treatment of obesity or diabetics and a food additive for regulating blood sugar levels. CONSTITUTION: A corn kernel or corn husk is extracted at 0 to 100deg.C in a polar organic solvent such as C1-4 lower alcohol, the extract is dissolved or suspended in water, an aqueous solution or aqueous suspension of the extract is brought into contact with a polar organic solvent or nonpolar organic solvent to disperse the extract component. Thereafter, the polar organic solvent solution or aqueous solution thereof is recovered, the polar organic solvent or water is removed therefrom to produce a partially purified corn extract.
Abstract translation: 目的：提供在玉米籽粒或玉米壳中提取玉米提取物的方法，极性有机溶剂或其混合物，然后从提取物中除去萃取溶剂。 因此，含有玉米提取物的组合物有效地用作预防和治疗肥胖或糖尿病的药物组合物和调节血糖水平的食品添加剂。 构成：在极性有机溶剂如C 1-4低级醇中，在0至100℃下提取玉米粒或玉米壳，将提取物溶解或悬浮于水中，将提取物的水溶液或水悬浮液 与极性有机溶剂或非极性有机溶剂接触以分散提取物组分。 此后，回收极性有机溶剂溶液或其水溶液，除去极性有机溶剂或水，得到部分纯化的玉米提取物。
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公开(公告)号：KR1020020085495A
公开(公告)日：2002-11-16
申请号：KR1020010025016
申请日：2001-05-08
Applicant: (주)바이오니아
IPC: C12Q1/68
Abstract: PURPOSE: A spotting pin for a microarray of biological samples is provided, to inhibit the damage of a board and the end of the pin due to their contact, and significantly increasing the amount of biological samples sucked. CONSTITUTION: The hollow typed spotting pin for a microarray of biological samples comprises a capillary part forming a tapered end and a reservoir part having larger inner diameter than the capillary part and containing a sample solution, wherein the inner diameter ratio of the capillary part to the reservoir part is 1:0.033 to 1:0.2; the inner diameters of the capillary part and the reservoir part are 0.01 to 0.5 mm and 0.5 to 3 mm, respectively; and the spotting pin is made from glass, soda line, or borosilicate glass.
Abstract translation: 目的：提供用于生物样品微阵列的点针，以抑制由于接触引起的板的损伤和引脚的末端，并显着增加吸附的生物样品的量。 构成：用于生物样品的微阵列的中空型点样针包括形成锥形端的毛细管部分和具有比毛细管部分更大的内径的容纳部分，并且包含样品溶液，其中毛细管部分与 储层部分为1：0.033〜1：0.2; 毛细管部分和储存部分的内径分别为0.01至0.5mm和0.5至3mm; 点焊针由玻璃，苏打线或硼硅酸盐玻璃制成。
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