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    通过使用染料化合物与乙烯基醚组合标记生物分子的方法

    公开(公告)号:KR1020130017111A

    公开(公告)日:2013-02-20

    申请号:KR1020110079332

    申请日:2011-08-10

    Abstract: PURPOSE: A method for labeling a biomolecule using a dye compound with a vinyl sulfone group is provided to effectively handle a dye. CONSTITUTION: A method for labeling a biomolecule comprises: a step of dissolving a dye containing a vinyl sulfone group in a first solvent and preparing a dye solution; a step of dissolving the biomolecule in a second solvent and preparing a biomolecule solution; a step of mixing the dye solution and the biomolecule solution and reacting the mixture solution; and a step of collecting solvents from the mixture solution and re-dissolving, filtering and purifying the remains. The first solvent is water or a solvent diluted with water.

    Abstract translation: 目的:提供使用具有乙烯基砜基团的染料化合物标记生物分子的方法以有效地处理染料。 构成:标记生物分子的方法包括:将含有乙烯基砜基的染料溶解在第一溶剂中并制备染料溶液的步骤; 将生物分子溶解在第二溶剂中并制备生物分子溶液的步骤; 混合染料溶液和生物分子溶液并使混合溶液反应的步骤; 以及从混合溶液中收集溶剂并重新溶解,过滤和净化残留物的步骤。 第一种溶剂是水或用水稀释的溶剂。

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