公开(公告)号：KR1020070118563A

公开(公告)日：2007-12-17

申请号：KR1020070108451

申请日：2007-10-26

Applicant: (주)셀트리온

Inventor： 김종묵 ,  홍혜진 ,  이현주 ,  소문경 ,  이수영 ,  조종문 ,  전복환 ,  홍승서 ,  조명삼

IPC: C12N15/85

CPC classification number: C12N15/85 ,  C12N2830/42 ,  C12N2830/46 ,  C12N2840/20

Abstract: An expression vector for animal cell is provided to increase expression efficiency of a foreign gene in an animal cell by introducing at least one copy of MAR(matrix attachment region) DNA sequences to the 3' terminal of a gene transcription termination region. An expression vector for animal cell comprises a operatively linked promoter, a polynucleotide encoding a cloning site or a foreign product, and a transcription termination region of which 3' terminal comprises at least two copy of human beta-globin gene MAR DNA sequences in which the MAR sequences are adjacent together or separated by a coding or non-coding sequence. The polynucleotide is LacZ(beta-galactosidase polypeptide), immunoglobulin, GCSF(granulocyte colony stimulating factor) or EPO(erythropoietin) and the animal cell is CHO(Chinese hamster ovary), BHK(baby hamster kidney), NSO(non-secreting mouse myeloma) or human cell.

Abstract translation: 提供用于动物细胞的表达载体，以通过将至少一个拷贝的MAR（基质附着区）DNA序列引入到基因转录终止区的3'末端来提高外源基因在动物细胞中的表达效率。 用于动物细胞的表达载体包括可操作地连接的启动子，编码克隆位点或外来产物的多核苷酸，以及转录终止区，其3'末端包含至少两个拷贝的人β-珠蛋白基因MAR DNA序列，其中 MAR序列相邻在一起或由编码或非编码序列分开。 多核苷酸是LacZ（β-半乳糖苷酶多肽），免疫球蛋白，GCSF（粒细胞集落刺激因子）或EPO（促红细胞生成素），动物细胞是CHO（中国仓鼠卵巢），BHK（小仓鼠肾），NSO（非分泌型小鼠 骨髓瘤）或人细胞。

公开(公告)号：KR101076602B1

公开(公告)日：2011-10-26

申请号：KR1020070108451

申请日：2007-10-26

Applicant: (주)셀트리온

Inventor： 김종묵 ,  홍혜진 ,  이현주 ,  소문경 ,  이수영 ,  조종문 ,  전복환 ,  홍승서 ,  조명삼

IPC: C12N15/85

CPC classification number: C12N15/85 ,  C12N2830/42 ,  C12N2830/46 ,  C12N2840/20

Abstract: 본발명은작동가능하게연결된프로모터, 클로닝부위또는외래산물을코딩하는폴리뉴클레오티드, 및전사종결인자를포함하는동물세포발현벡터로서, 상기전사종결인자의 3′말단에 1 카피이상의인간베타글로빈유전자 MAR (matrix attachment region) 서열을포함하는동물세포발현벡터및 그를이용하여외래유전자를발현하는방법을제공한다.